Carole Fruchart-Gaillard

Summary

Country: France

Publications

  1. pmc High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli
    Hervé Nozach
    CEA, iBiTec S, Service d Ingénierie Moléculaire des Protéines, CEA Saclay, Gif Sur Yvette F 91191, France
    Microb Cell Fact 12:37. 2013
  2. pmc Deacetylation of H4-K16Ac and heterochromatin assembly in senescence
    Kévin Contrepois
    CEA, iBiTecS, Service de Biologie Intégrative et de Génétique Moléculaire SBIGeM, F 91191, Gif sur Yvette, France
    Epigenetics Chromatin 5:15. 2012
  3. doi request reprint Mass spectrometric characterization of N- and O-glycans of plasma-derived coagulation factor VII
    François Fenaille
    Laboratoire Francais du Fractionnement et des Biotechnologies, 3 avenue des Tropiques, BP 305, Les Ulis, 91958, Courtaboeuf Cedex, France
    Glycoconj J 25:827-42. 2008
  4. doi request reprint Mass spectrometric investigation of molecular variability of grass pollen group 1 allergens
    François Fenaille
    CEA, iBitec S Service de Pharmacologie et d Immunoanalyse, Gif sur Yvette, France
    J Proteome Res 8:4014-27. 2009
  5. ncbi request reprint Site-specific N-glycan characterization of human complement factor H
    François Fenaille
    Laboratoire Francais du Fractionnement et des Biotechnologies, Développement Biopharmaceutique, 3 avenue des Tropiques, BP305 Les Ulis, 91958 Courtaboeuf cedex, France
    Glycobiology 17:932-44. 2007
  6. doi request reprint Rapid quantification of clostridial epsilon toxin in complex food and biological matrixes by immunopurification and ultraperformance liquid chromatography-tandem mass spectrometry
    Alexandre Seyer
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
    Anal Chem 84:5103-9. 2012
  7. doi request reprint Mass spectrometry protocol for the absolute quantification of a monoclonal antibody in serum with immunopurification
    François Becher
    Service de Pharmacologie et d immunoanalyse, CEA, Gif sur Yvette, France
    Methods Mol Biol 988:345-52. 2013
  8. doi request reprint MS-based approaches to unravel the molecular complexity of proprotein-derived biomarkers and support their quantification: the examples of B-type natriuretic peptide and apelin peptides
    Cédric Mesmin
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, F 91191 Gif sur Yvette, France
    Bioanalysis 4:2851-63. 2012
  9. doi request reprint Identification and characterization of apelin peptides in bovine colostrum and milk by liquid chromatography-mass spectrometry
    Cédric Mesmin
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, France
    J Proteome Res 10:5222-31. 2011
  10. doi request reprint Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Elodie Duriez
    CEA, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
    J Proteome Res 7:4154-63. 2008

Collaborators

  • Hervé Volland
  • Eric Ezan
  • Michel R Popoff
  • Gilles Clement
  • Nathalie Morel
  • Aurélie Lautrette
  • Hélène Manduzio
  • François Fenaille
  • François Becher
  • Cédric Mesmin
  • Kévin Contrepois
  • Mathieu Dubois
  • Jean Claude Tabet
  • Patricia Lamourette
  • Hervé Nozach
  • Alexandre Seyer
  • Jérôme Chenau
  • Carl Mann
  • Nathalie Khreich
  • Elodie Duriez
  • Catherine Groseil
  • Christine Ramon
  • Laurent Siret
  • Sandrine Riandé
  • Nicolas Bihoreau
  • Fabrice Beau
  • Natalie J Saez
  • Badreddine Douzi
  • Robert Thai
  • Vincent Dive
  • Denis Servent
  • Mireille Moutiez
  • Philippe Cuniasse
  • Renaud Vincentelli
  • Oscar Henrique Pereira Ramos
  • Muriel Gondry
  • Christophe Junot
  • Margaux Renvoisé
  • Cécile Féraudet-Tarisse
  • Régis Courbeyrette
  • Jean Yves Thuret
  • Pierre L Goossens
  • Pierre Yves Renard
  • Emmanuel Nony
  • Marie Noëlle Couret
  • Thierry Batard
  • Philippe Moingeon
  • Christophe Creminon
  • Guillaume Clavé
  • Henri Chabre
  • Martin Lechmann
  • Sami Chtourou
  • Didier Hilaire
  • Maxime Le Mignon

Detail Information

Publications17

  1. pmc High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli
    Hervé Nozach
    CEA, iBiTec S, Service d Ingénierie Moléculaire des Protéines, CEA Saclay, Gif Sur Yvette F 91191, France
    Microb Cell Fact 12:37. 2013
    ..Recombinant expression of such small proteins containing multiple disulfide bonds in the bacteria E. coli is considered difficult and general methods and protocols, particularly on a high throughput scale, are limited...
  2. pmc Deacetylation of H4-K16Ac and heterochromatin assembly in senescence
    Kévin Contrepois
    CEA, iBiTecS, Service de Biologie Intégrative et de Génétique Moléculaire SBIGeM, F 91191, Gif sur Yvette, France
    Epigenetics Chromatin 5:15. 2012
    ..abstract:..
  3. doi request reprint Mass spectrometric characterization of N- and O-glycans of plasma-derived coagulation factor VII
    François Fenaille
    Laboratoire Francais du Fractionnement et des Biotechnologies, 3 avenue des Tropiques, BP 305, Les Ulis, 91958, Courtaboeuf Cedex, France
    Glycoconj J 25:827-42. 2008
    ..These latter three O-glycans coexist in equal amounts in plasma-derived FVII...
  4. doi request reprint Mass spectrometric investigation of molecular variability of grass pollen group 1 allergens
    François Fenaille
    CEA, iBitec S Service de Pharmacologie et d Immunoanalyse, Gif sur Yvette, France
    J Proteome Res 8:4014-27. 2009
    ..Lastly, natural or process-induced modifications (deamidation, oxidation, glycation) were evidenced, which might constitute useful indicators of product degradation...
  5. ncbi request reprint Site-specific N-glycan characterization of human complement factor H
    François Fenaille
    Laboratoire Francais du Fractionnement et des Biotechnologies, Développement Biopharmaceutique, 3 avenue des Tropiques, BP305 Les Ulis, 91958 Courtaboeuf cedex, France
    Glycobiology 17:932-44. 2007
    ..Interestingly, the sialylation level along with the amount of triantennary structures decreases from the N- to the C-terminal side of the protein...
  6. doi request reprint Rapid quantification of clostridial epsilon toxin in complex food and biological matrixes by immunopurification and ultraperformance liquid chromatography-tandem mass spectrometry
    Alexandre Seyer
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
    Anal Chem 84:5103-9. 2012
    ..The assay sensitivity in milk and serum was estimated at 5 ng·mL(-1) for ETX, making this approach complementary to enzyme linked immunosorbent assay (ELISA) techniques...
  7. doi request reprint Mass spectrometry protocol for the absolute quantification of a monoclonal antibody in serum with immunopurification
    François Becher
    Service de Pharmacologie et d immunoanalyse, CEA, Gif sur Yvette, France
    Methods Mol Biol 988:345-52. 2013
    ..The limit of quantification of the assay was 20 ng/mL in serum...
  8. doi request reprint MS-based approaches to unravel the molecular complexity of proprotein-derived biomarkers and support their quantification: the examples of B-type natriuretic peptide and apelin peptides
    Cédric Mesmin
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, F 91191 Gif sur Yvette, France
    Bioanalysis 4:2851-63. 2012
    ..Two particular examples are discussed in detail: BNP-related peptides and some more putative biomarkers of heart failure, the apelin peptides...
  9. doi request reprint Identification and characterization of apelin peptides in bovine colostrum and milk by liquid chromatography-mass spectrometry
    Cédric Mesmin
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, France
    J Proteome Res 10:5222-31. 2011
    ..Taken together, our data demonstrate that the multiplicity and variability of apelin peptides are biologically relevant and change during milk maturation to reach a more constant composition in mature milk...
  10. doi request reprint Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Elodie Duriez
    CEA, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
    J Proteome Res 7:4154-63. 2008
    ..The present assay provides a new tool with a total analytical time of approximately 5 h, which is particularly relevant in the context of a bioterrorist incident...
  11. doi request reprint Liquid chromatography/tandem mass spectrometry assay for the absolute quantification of the expected circulating apelin peptides in human plasma
    Cédric Mesmin
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
    Rapid Commun Mass Spectrom 24:2875-84. 2010
    ..These results question the nature and/or the concentration of circulating apelin peptides as well as the specificity of the immunoassays that have hitherto been used for clinical applications...
  12. doi request reprint Evaluation of the LTQ-Orbitrap mass spectrometer for the analysis of polymerase chain reaction products
    Hélène Manduzio
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
    Rapid Commun Mass Spectrom 24:3501-9. 2010
    ..For example, a single (T ↔ A) switch (9 Da mass difference) was successfully identified in a 114-bp PCR product...
  13. doi request reprint Immunopurification and mass spectrometric quantification of the active form of a chimeric therapeutic antibody in human serum
    Mathieu Dubois
    CEA, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France, INRA, Laboratoire d Immuno Allergie Alimentaire, 91191 Gif sur Yvette, France
    Anal Chem 80:1737-45. 2008
    ..The 20 ng/mL lower limit of quantification was similar to that of ELISA methods. These results show that this mass spectrometric approach is a potential alternative for pharmacokinetic evaluation of mAbs during clinical development...
  14. doi request reprint Sensitive detection of Bacillus anthracis spores by immunocapture and liquid chromatography-tandem mass spectrometry
    Jérôme Chenau
    Service de Pharmacologie et d immunoanalyse, iBiTec S, CEA, Gif sur Yvette Cedex, France
    Anal Chem 83:8675-82. 2011
    ..This mass spectrometry approach is thus complementary to polymerase chain reaction (PCR) techniques...
  15. doi request reprint Ultra-high performance liquid chromatography-mass spectrometry for the fast profiling of histone post-translational modifications
    Kévin Contrepois
    CEA, iBiTecS, Service de Biologie Intégrative et Génétique Moléculaire SBIGeM, Gif sur Yvette, France
    J Proteome Res 9:5501-9. 2010
    ..Overall, our UHPLC-MS approach for histone profiling offers a sensitive and reproducible tool that will be of great value for exploring PTMs and variants and can readily be applied to clinical or pharmaceutical studies...
  16. doi request reprint A highly sensitive competitive enzyme immunoassay of broad specificity quantifying microcystins and nodularins in water samples
    Nathalie Khreich
    CEA, iBiTecS, Service de Pharmacologie et d immunoanalyse, Gif sur Yvette, F 91191, France
    Toxicon 53:551-9. 2009
    ..Indeed, in parallel analysis of these samples using HPLC, EIA shows a good relationship between both measurements while LC-MS/MS demonstrates the presence of different variants of MCs whose heterogeneity did not impair EIA measurement...
  17. doi request reprint Comparison of approaches for purifying and desalting polymerase chain reaction products prior to electrospray ionization mass spectrometry
    Hélène Manduzio
    Commissariat a l Energie Atomique, iBiTec S, Service de Pharmacologie et d immunoanalyse, Gif sur Yvette, France
    Anal Biochem 398:272-4. 2010
    ....