Agnes Ferroni

..Their efficiency is low, and there is still a need to look for other arguments of diagnosis such as search of possible portals of entry or specific serologies...

..50%] versus 27 [3.25%]; P < 0.0001) despite a higher contamination rate (20% versus 14.2%; P = 0.0017)...

..MALDI-TOF-MS is currently the fastest technique to accurately identify microorganisms grown in positive blood culture broths...

..We determined the relationship between the presence of P. aeruginosa mutator strains and cystic fibrosis clinical characteristics...

..Altogether, these data demonstrate that, in CF patients, MALDI-TOF-MS is a powerful tool for rapid identification of nonfermenting gram-negative bacilli...

..The diagnosis and prognosis of lung infections due to the emerging nontuberculous mycobacterium (NTM) Mycobacterium abscessus are difficult to establish in children with cystic fibrosis...

..We therefore recommend to open windows after physiotherapy and to implement a strong decontamination after showers...

..Real-time PCR technique is a sensitive and reliable technique, which can replace conventional PCR for clinical specimens with negative bacterial culture...

..No misidentification was noted. Our results, based on a very simple protocol, suggest that this system may represent a serious alternative for clinical laboratories to identify mycobacterial species...

..To study the bacterial aetiology of acute osteoarticular infections in children and to analyse the efficiency of culture methods...

..Broad-range 16S rDNA PCR has value in detecting bacterial agents responsible for culture-negative pleural empyema...

..The goal of this review was to update recent data concerning routine identification of microorganisms by MALDI-TOF in the clinical microbiology laboratory...

..Similar results were obtained using four different growth conditions. Extending this strategy to other groups of relevant pathogenic bacteria will allow rapid bacterial identification...

..Overall, compliance with the consensus recommendations was poor. This was partly due to the high turnover rate of antibiotic prescribers...

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..To compare cefoxitin and/or moxalactam 30 microg disc diffusion (DD) methods to detect methicillin resistance in coagulase-negative staphylococci (CoNS) using both high- and low-density (HD/LD) inoculum techniques...

..However, the risk of false-positive results remains quite high, and we strongly recommend establishment of uniform quality control procedures performed regularly...

..Here we report the first case of Streptococcus pneumoniae endophthalmitis diagnosed by polymerase chain reaction. Molecular biology should be considered as a new tool for the diagnosis of such sequestered sites as the eye...

..However, information is lacking about factors associated with prolonged MRSA colonization and MRSA transmission to household contacts...

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..The aim of this article is to review the evolution of therapeutic concepts of treatment of bone and joint infections in paediatrics for 40 years with the aid of main published clinical studies...

..This prolonged antibiotherapy must be monitored because of possible induction of bacterial resistance, nephrotoxicity and ototoxicity of aminosids and allergy to beta-lactams...

..In the late 1980s, the proportion of methicillin resistance among clinical strains of Staphylococcus aureus (MRSA) reached approximately 40% at AP-HP...

..To assess the probability of achieving ciprofloxacin pharmacodynamic targets with currently recommended dosages in order to investigate the risk of ciprofloxacin underexposure in children with cystic fibrosis...

..These cases emphasize the necessity of respecting basic hygiene rules and the danger involved in animal sacrifice without suitable professional structure...

..Aerosol maintenance treatment with an appropriate antibiotic in a high enough dosage can be recommended for patients with CF who are chronically infected with P. aeruginosa...

..2%) and erythromycin (4.5%), followed by serotypes 19F and 14. We confirm the predominance of serotype 19A among S. pneumoniae responsible for invasive and noninvasive diseases either in children or adults in France...

..Although antibiotic use may be considered as an adjunct to surgical intervention of CA, the appropriate use of preoperative antibiotics is essential and must be constantly reevaluated according to the bacterial epidemiology...

..The knowledge of the typability of the strains and the qualities of these various methods, particularly their discriminatory power, is essential to the accuracy of the laboratory analysis in the investigations of outbreaks...

..Arbitrarily primed PCR analysis showed a clonal strain. The repeated septic episodes were resolved with the catheter repair...

..In addition, a decline in IFNgamma values during treatment makes it possible for clinicians to monitor the effect of preventive or curative therapy...

..Campylobacter bacteremia is uncommon. The influence of underlying conditions and of the impact of antibiotics on infection outcome are not known...

..However, 10% of meals, all of which were salads, had total viable counts of bacteria above the recommended limits. This study confirms that it is essential to control time and temperature to ensure food quality and safety in hospitals...

..A B. cepacia strain genotypically identical to the blood isolates was recovered from the upper surface of capped rubber stoppers of bottles of a commercial lipid emulsion used for parenteral nutrition...

..Alterations were detected in an isolate with a penicillin MIC as low as 0.06 mg/L. These results confirm that the level of penicillin resistance in pneumococci reflects with sequential alterations of PBPs in clinical isolates...

..This combination was shown to be synergistic. It has minimal side effects in transplant recipients and appears to be an effective initial treatment for this severe infection...

..The genotypic methods, such as 16S DNA sequencing which allows identification of strains in routine practice, appears to have a small, but significant impact on the clinical management of CF patients...