Research Topics
Species | François FenailleSummaryCountry: France Publications
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Detail Information
Publications
Mass spectrometric characterization of N- and O-glycans of plasma-derived coagulation factor VIIFrançois Fenaille
Laboratoire Francais du Fractionnement et des Biotechnologies, 3 avenue des Tropiques, BP 305, Les Ulis, 91958, Courtaboeuf Cedex, France
Glycoconj J 25:827-42. 2008..These latter three O-glycans coexist in equal amounts in plasma-derived FVII...
Mass spectrometric investigation of molecular variability of grass pollen group 1 allergensFrançois Fenaille
CEA, iBitec S Service de Pharmacologie et d Immunoanalyse, Gif sur Yvette, France
J Proteome Res 8:4014-27. 2009..Lastly, natural or process-induced modifications (deamidation, oxidation, glycation) were evidenced, which might constitute useful indicators of product degradation...
Site-specific N-glycan characterization of human complement factor HFrançois Fenaille
Laboratoire Francais du Fractionnement et des Biotechnologies, Développement Biopharmaceutique, 3 avenue des Tropiques, BP305 Les Ulis, 91958 Courtaboeuf cedex, France
Glycobiology 17:932-44. 2007..Interestingly, the sialylation level along with the amount of triantennary structures decreases from the N- to the C-terminal side of the protein...
Rapid quantification of clostridial epsilon toxin in complex food and biological matrixes by immunopurification and ultraperformance liquid chromatography-tandem mass spectrometryAlexandre Seyer
CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
Anal Chem 84:5103-9. 2012..The assay sensitivity in milk and serum was estimated at 5 ng·mL(-1) for ETX, making this approach complementary to enzyme linked immunosorbent assay (ELISA) techniques...
Identification and characterization of apelin peptides in bovine colostrum and milk by liquid chromatography-mass spectrometryCédric Mesmin
CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, France
J Proteome Res 10:5222-31. 2011..Taken together, our data demonstrate that the multiplicity and variability of apelin peptides are biologically relevant and change during milk maturation to reach a more constant composition in mature milk...
Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometryElodie Duriez
CEA, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
J Proteome Res 7:4154-63. 2008..The present assay provides a new tool with a total analytical time of approximately 5 h, which is particularly relevant in the context of a bioterrorist incident...
Evaluation of the LTQ-Orbitrap mass spectrometer for the analysis of polymerase chain reaction productsHélène Manduzio
CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
Rapid Commun Mass Spectrom 24:3501-9. 2010..For example, a single (T ↔ A) switch (9 Da mass difference) was successfully identified in a 114-bp PCR product...
Liquid chromatography/tandem mass spectrometry assay for the absolute quantification of the expected circulating apelin peptides in human plasmaCédric Mesmin
CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France
Rapid Commun Mass Spectrom 24:2875-84. 2010..These results question the nature and/or the concentration of circulating apelin peptides as well as the specificity of the immunoassays that have hitherto been used for clinical applications...
MS-based approaches to unravel the molecular complexity of proprotein-derived biomarkers and support their quantification: the examples of B-type natriuretic peptide and apelin peptidesCédric Mesmin
CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, F 91191 Gif sur Yvette, France
Bioanalysis 4:2851-63. 2012..Two particular examples are discussed in detail: BNP-related peptides and some more putative biomarkers of heart failure, the apelin peptides...
Sensitive detection of Bacillus anthracis spores by immunocapture and liquid chromatography-tandem mass spectrometryJérôme Chenau
Service de Pharmacologie et d immunoanalyse, iBiTec S, CEA, Gif sur Yvette Cedex, France
Anal Chem 83:8675-82. 2011..This mass spectrometry approach is thus complementary to polymerase chain reaction (PCR) techniques...
Immunopurification and mass spectrometric quantification of the active form of a chimeric therapeutic antibody in human serumMathieu Dubois
CEA, Service de Pharmacologie et d immunoanalyse, 91191 Gif sur Yvette, France, INRA, Laboratoire d Immuno Allergie Alimentaire, 91191 Gif sur Yvette, France
Anal Chem 80:1737-45. 2008..The 20 ng/mL lower limit of quantification was similar to that of ELISA methods. These results show that this mass spectrometric approach is a potential alternative for pharmacokinetic evaluation of mAbs during clinical development...
Ultra-high performance liquid chromatography-mass spectrometry for the fast profiling of histone post-translational modificationsKévin Contrepois
CEA, iBiTecS, Service de Biologie Intégrative et Génétique Moléculaire SBIGeM, Gif sur Yvette, France
J Proteome Res 9:5501-9. 2010..Overall, our UHPLC-MS approach for histone profiling offers a sensitive and reproducible tool that will be of great value for exploring PTMs and variants and can readily be applied to clinical or pharmaceutical studies...
Deacetylation of H4-K16Ac and heterochromatin assembly in senescenceKévin Contrepois
CEA, iBiTecS, Service de Biologie Intégrative et de Génétique Moléculaire SBIGeM, F 91191, Gif sur Yvette, France
Epigenetics Chromatin 5:15. 2012..abstract:..
A highly sensitive competitive enzyme immunoassay of broad specificity quantifying microcystins and nodularins in water samplesNathalie Khreich
CEA, iBiTecS, Service de Pharmacologie et d immunoanalyse, Gif sur Yvette, F 91191, France
Toxicon 53:551-9. 2009..Indeed, in parallel analysis of these samples using HPLC, EIA shows a good relationship between both measurements while LC-MS/MS demonstrates the presence of different variants of MCs whose heterogeneity did not impair EIA measurement...
Comparison of approaches for purifying and desalting polymerase chain reaction products prior to electrospray ionization mass spectrometryHélène Manduzio
Commissariat a l Energie Atomique, iBiTec S, Service de Pharmacologie et d immunoanalyse, Gif sur Yvette, France
Anal Biochem 398:272-4. 2010....
