P Fach

Summary

Country: France

Publications

  1. pmc Detection by PCR-enzyme-linked immunosorbent assay of Clostridium botulinum in fish and environmental samples from a coastal area in northern France
    Patrick Fach
    Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments LERHQA, Agence Française de Sécurité Sanitaire des Aliments AFSSA, Unité ATB, 94700 Maisons Alfort, France
    Appl Environ Microbiol 68:5870-6. 2002
  2. ncbi request reprint Detection of genetically modified corn (Bt176) in spiked cow blood samples by polymerase chain reaction and immunoassay methods
    Laetitia Petit
    Agence Francaise de Securite Sanitaire des Aliments, Unité d Etude Moléculaire des Contaminants Biologiques Alimentaires EBA, 94700 Maisons Alfort, France
    J AOAC Int 88:654-64. 2005
  3. ncbi request reprint Comparison between a PCR-ELISA test and the vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains
    P Fach
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 90:809-18. 2001
  4. doi request reprint An innovative molecular detection tool for tracking and tracing Clostridium botulinum types A, B, E, F and other botulinum neurotoxin producing Clostridia based on the GeneDisc cycler
    P Fach
    French Food Safety Agency, Laboratory for Study and Research on Food Quality and Processes, 23 Av du Général De Gaulle, Fr 94706 Maisons Alfort, France
    Int J Food Microbiol 145:S145-51. 2011
  5. doi request reprint Development of real-time PCR tests for detecting botulinum neurotoxins A, B, E, F producing Clostridium botulinum, Clostridium baratii and Clostridium butyricum
    P Fach
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et les Procédés Agro alimentaires, Maisons Alfort, France
    J Appl Microbiol 107:465-73. 2009
  6. ncbi request reprint Comparison of different PCR tests for detecting Shiga toxin-producing Escherichia coli O157 and development of an ELISA-PCR assay for specific identification of the bacteria
    Patrick Fach
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, 1 5 rue de Belfort, 94700 Maisons Alfort, France
    J Microbiol Methods 55:383-92. 2003
  7. ncbi request reprint Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction
    S Perelle
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d études et de recherches sur la qualité des aliments et sur les procédés agroalimentaires, Unité EBA Etude moléculaire des contaminants biologiques alimentaires, Maisons Alfort, France
    J Appl Microbiol 98:1162-8. 2005
  8. doi request reprint Duplex real-time PCR detection of type III effector tccP and tccP2 genes in pathogenic Escherichia coli and prevalence in raw milk cheeses
    J Madic
    Anses, Maisons Alfort Laboratory for Food Safety, 23 Avenue du Général de Gaulle, Maisons Alfort, France
    Lett Appl Microbiol 52:538-45. 2011
  9. ncbi request reprint Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue
    S Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 94:587-94. 2003
  10. doi request reprint Screening of food raw materials for the presence of Shiga toxin-producing Escherichia coli O91:H21
    J Madic
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et les Procédés agroalimentaires, Maisons Alfort, France
    Lett Appl Microbiol 48:447-51. 2009

Collaborators

Detail Information

Publications31

  1. pmc Detection by PCR-enzyme-linked immunosorbent assay of Clostridium botulinum in fish and environmental samples from a coastal area in northern France
    Patrick Fach
    Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments LERHQA, Agence Française de Sécurité Sanitaire des Aliments AFSSA, Unité ATB, 94700 Maisons Alfort, France
    Appl Environ Microbiol 68:5870-6. 2002
    ..botulinum type E reported in many worldwide and northern European surveys. However, fish processing and fish preparation in France have not been identified as a significant hazard for human type B botulism...
  2. ncbi request reprint Detection of genetically modified corn (Bt176) in spiked cow blood samples by polymerase chain reaction and immunoassay methods
    Laetitia Petit
    Agence Francaise de Securite Sanitaire des Aliments, Unité d Etude Moléculaire des Contaminants Biologiques Alimentaires EBA, 94700 Maisons Alfort, France
    J AOAC Int 88:654-64. 2005
    ..Both methods detected a minimum spiking at 25 copies of Bt176 maize/mL in 10 mL whole blood samples. The sandwich ELISA kit used detected down to 1 ng transgenic Cry1Ab protein/mL spiked whole blood...
  3. ncbi request reprint Comparison between a PCR-ELISA test and the vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains
    P Fach
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 90:809-18. 2001
    ..This paper provides information on a PCR-ELISA method for detecting Shiga toxin-producing Escherichia coli (STEC), and on their prevalence in dairy products...
  4. doi request reprint An innovative molecular detection tool for tracking and tracing Clostridium botulinum types A, B, E, F and other botulinum neurotoxin producing Clostridia based on the GeneDisc cycler
    P Fach
    French Food Safety Agency, Laboratory for Study and Research on Food Quality and Processes, 23 Av du Général De Gaulle, Fr 94706 Maisons Alfort, France
    Int J Food Microbiol 145:S145-51. 2011
    ..The test is robust, has a low detection limit (c.a. 5 to 50 genome copies in the PCR reaction microwell) and is promising for monitoring BoNT-producing Clostridia in different kinds of samples including food and clinical samples...
  5. doi request reprint Development of real-time PCR tests for detecting botulinum neurotoxins A, B, E, F producing Clostridium botulinum, Clostridium baratii and Clostridium butyricum
    P Fach
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et les Procédés Agro alimentaires, Maisons Alfort, France
    J Appl Microbiol 107:465-73. 2009
    ..To develop real-time PCR assays for tracking and tracing clostridia responsible for human botulism...
  6. ncbi request reprint Comparison of different PCR tests for detecting Shiga toxin-producing Escherichia coli O157 and development of an ELISA-PCR assay for specific identification of the bacteria
    Patrick Fach
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, 1 5 rue de Belfort, 94700 Maisons Alfort, France
    J Microbiol Methods 55:383-92. 2003
    ..Adaptation of PCR into ELISA-PCR assay format facilitates specific and sensitive detection of PCR amplification products and constitutes a method of choice for screening STEC O157...
  7. ncbi request reprint Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction
    S Perelle
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d études et de recherches sur la qualité des aliments et sur les procédés agroalimentaires, Unité EBA Etude moléculaire des contaminants biologiques alimentaires, Maisons Alfort, France
    J Appl Microbiol 98:1162-8. 2005
    ..The aims of the study were to identify the specific genes of O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O103 and to provide the basis for a specific real-time PCR test for rapid detection of E. coli O103...
  8. doi request reprint Duplex real-time PCR detection of type III effector tccP and tccP2 genes in pathogenic Escherichia coli and prevalence in raw milk cheeses
    J Madic
    Anses, Maisons Alfort Laboratory for Food Safety, 23 Avenue du Général de Gaulle, Maisons Alfort, France
    Lett Appl Microbiol 52:538-45. 2011
    ....
  9. ncbi request reprint Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue
    S Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 94:587-94. 2003
    ..A DNA sequence, from Escherichia coli STEC O145, homologous to O-island 29 from STEC O157 is described, together with a real-time PCR assay for detecting it...
  10. doi request reprint Screening of food raw materials for the presence of Shiga toxin-producing Escherichia coli O91:H21
    J Madic
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et les Procédés agroalimentaires, Maisons Alfort, France
    Lett Appl Microbiol 48:447-51. 2009
    ..To provide information on the prevalence and detection, in foods, of Shiga toxin-producing Escherichia coli (STEC) O91:H21...
  11. ncbi request reprint Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus
    C Letertre
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 95:38-43. 2003
    ..This paper reports on a new putative enterotoxin SEU encoded by the enterotoxin gene cluster egc from Staphylococcus aureus and on a real-time polymerase chain reaction (PCR) assay for detecting the seu gene...
  12. doi request reprint Simplex and multiplex real-time PCR assays for the detection of flagellar (H-antigen) fliC alleles and intimin (eae) variants associated with enterohaemorrhagic Escherichia coli (EHEC) serotypes O26:H11, O103:H2, O111:H8, O145:H28 and O157:H7
    J Madic
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et sur les Procédés agroalimentaires AFSSA LERQAP, Maisons Alfort, France
    J Appl Microbiol 109:1696-705. 2010
    ..e. O26:H11, O103:H2, O111:H8, O145:H28 and O157:H7...
  13. ncbi request reprint Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test
    S Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 93:758-64. 2002
    ..The aims of the study were to characterize the O91 O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91...
  14. ncbi request reprint Development of a 5'-nuclease PCR assay for the identification of Escherichia coli strains expressing the flagellar antigen H21 and their detection in food after enrichment
    F Auvray
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et les Procédés agroalimentaires AFSSA LERQAP, Maisons Alfort, France
    J Appl Microbiol 104:899-905. 2008
    ..To develop a real-time PCR assay targeting the Escherichia coli flagellar antigen H21 for identification and surveillance of clinically important Shiga toxin-producing E. coli (STEC) serotypes classified in seropathotype C...
  15. ncbi request reprint A PCR test for detecting Escherichia coli O157:H7 based on the identification of the small inserted locus (SILO157)
    S Perelle
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, Maisons Alfort, France
    J Appl Microbiol 93:250-60. 2002
    ..This paper provides identification of a DNA sequence derived from Shiga toxin-producing Escherichia coli (STEC) O157:H7 and information on its utilization for detecting STEC O157 by PCR...
  16. ncbi request reprint Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination
    Laetitia Petit
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et sur les Procédés Agro alimentaires, Unité EBA, 23 Avenue du Général de Gaulle, 94700 Maisons Alfort, France
    J AOAC Int 90:1098-106. 2007
    ..Finally, analysis of SSR markers indicated that the contaminations were essentially due to cross-pollination in the seed production process...
  17. ncbi request reprint Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157
    Sylvie Perelle
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d études et de recherches sur la qualité des aliments et sur les procédés agroalimentaires, Unité EBA Etude moléculaire des contaminants biologiques alimentaires, Maisons Alfort, France
    Int J Food Microbiol 113:284-8. 2007
    ..This rapid screening approach for evaluating the potential presence of highly pathogenic serogroups of STEC in food raw materials should help to improve risk assessment of food poisoning outbreaks...
  18. doi request reprint Use of a robotic RNA purification protocol based on the NucliSens easyMAG for real-time RT-PCR detection of hepatitis A virus in bottled water
    Sylvie Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, 94706 Maisons Alfort cedex, France
    J Virol Methods 157:80-3. 2009
    ..The limit of detection (LOD) with the new rapid simplified NucliSens easyMAG approach was about 1PFU/1.5L against about 100PFU/1.5L using conventional sample treatments that included a concentration step using ultra-filtration...
  19. pmc Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): a new approach for molecular risk assessment of STEC isolates
    Marie Bugarel
    French Food Safety Agency, Food Quality and Agro alimentary Processes Research Laboratory, avenue du General de Gaulle, F 94706 Maisons Alfort, France
    Appl Environ Microbiol 76:203-11. 2010
    ..The presence of eae, ent/espL2, nleB, nleE, and nleH1-2 genes is a clear signature of STEC strains with high virulence for humans...
  20. doi request reprint Persistence of plant DNA sequences in the blood of dairy cows fed with genetically modified (Bt176) and conventional corn silage
    Y Bertheau
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et les Procédés Agro alimentaires, Maisons Alfort, France
    J Agric Food Chem 57:509-16. 2009
    ..Those results show that in order to meet the consumers' demand of animals fed with GM products there is currently no cost-effective analytical procedure to replace documentary traceability...
  21. ncbi request reprint Evaluation of the performance of LNA and MGB probes in 5'-nuclease PCR assays
    Capucine Letertre
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, 1 5, rue de Belfort, 94700 Maisons Alfort, France
    Mol Cell Probes 17:307-11. 2003
    ....
  22. ncbi request reprint A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter
    Sylvie Perelle
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d études et de recherches sur la qualité des aliments et sur les procédés agroalimentaires, Maisons Alfort 94700, France
    Mol Cell Probes 18:321-7. 2004
    ....
  23. ncbi request reprint A strategy based on 5' nuclease multiplex PCR to detect enterotoxin genes sea to sej of Staphylococcus aureus
    Capucine Letertre
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Unité Atelier de Biotechnologie, 1 5 rue de Belfort, Maisons Alfort 94700, France
    Mol Cell Probes 17:227-35. 2003
    ..The development of this method is an improvement that should facilitate epidemiological investigations of staphylococcal food poisoning outbreaks...
  24. ncbi request reprint Detection and genotyping by real-time PCR of the staphylococcal enterotoxin genes sea to sej
    Capucine Letertre
    Unité Atelier de Biotechnologie, Laboratoire d Etudes et de Recherches sur l Hygiène et la Qualité des Aliments, Agence Française de Sécurité Sanitaire des Aliments AFSSA, 1 5 rue de Belfort, 94700, Maisons Alfort, France
    Mol Cell Probes 17:139-47. 2003
    ..aureus. The work offers a very quick, reliable and specific alternative to conventional block cycler PCR assays to identify the enterotoxin profile of toxigenic S. aureus...
  25. pmc Diagnostic real-time PCR for detection of Salmonella in food
    Burkhard Malorny
    Federal Institute for Risk Assessment, Berlin, Germany
    Appl Environ Microbiol 70:7046-52. 2004
    ..This methodology can contribute to meeting the increasing demand of quality assurance laboratories for standard diagnostic methods. Studies are planned to assess the interlaboratory performance of this diagnostic PCR method...
  26. ncbi request reprint Detection by 5'-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world's most frequent clinical cases
    Sylvie Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d études et de recherches sur la qualité des aliments et sur les procédés agroalimentaires, Unité Etude moléculaire des contaminants biologiques alimentaires, Maisons Alfort, France
    Mol Cell Probes 18:185-92. 2004
    ..coli. This work provides sensitive and specific tests for the rapid, reliable detection of the main pathogenic E. coli O-serogroups of major public health concern...
  27. ncbi request reprint Prevalence of Clostridium botulinum in food raw materials used in REPFEDs manufactured in France
    Frédéric Carlin
    Institut National de la Recherche Agronomique INRA, UMR A408 Sécurité et Qualité des Produits d Origine Végétale, Site Agroparc, 84914 Avignon Cedex 9, France
    Int J Food Microbiol 91:141-5. 2004
    ..Of the 28 samples positive in PCR-ELISA, 15 were also positive in the mouse bioassay. The MPN count was between 1 and 3 C. botulinum/kg of food, which is similar to or in the lower range of values reported in the literature...
  28. ncbi request reprint Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples
    Sylvie Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et sur les Procédés Agroalimentaires LERQAP, 1 5 rue de Belfort, 94700 Maisons Alfort, France
    Mol Cell Probes 18:409-20. 2004
    ..The results on naturally contaminated samples demonstrated that despite certain inhibition problems, LC-PCR and PCR-ELISA assays were highly specific and sensitive, and provide a powerful tool for detection of Salmonella in food samples...
  29. pmc Making internal amplification control mandatory for diagnostic PCR
    Jeffrey Hoorfar
    J Clin Microbiol 41:5835. 2003
  30. ncbi request reprint Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method
    Burkhard Malorny
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    Int J Food Microbiol 89:241-9. 2003
    ..This work can contribute to the quality assurance of PCR-based diagnostic methods and is currently proposed as international standard document...
  31. ncbi request reprint Multicenter validation of PCR-based method for detection of Salmonella in chicken and pig samples
    Burkhard Malorny
    Federal Institute for Risk Assessment, National Salmonella Reference Laboratory, Diedersdorfer Weg, Berlin, Germany
    J AOAC Int 87:861-6. 2004
    ..3% concordance) for pig swab. Thus, the interlaboratory variation due to personnel, reagents, thermal cyclers, etc., did not affect the performance of the method, which will be proposed as part of a developing international PCR standard...