Winfried Weissenhorn

Summary

Affiliation: European Molecular Biology Laboratory
Country: France

Publications

  1. pmc Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p
    Andreas Bracher
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    EMBO J 21:6114-24. 2002
  2. ncbi request reprint Filovirus assembly and budding
    Bettina Hartlieb
    Institut fur Virologie, Robert Koch str 17, 35037 Marburg, Germany
    Virology 344:64-70. 2006
  3. ncbi request reprint ESCRT-I part II: forming the real ESCRT-I complex
    Winfried Weissenhorn
    Unit for Virus Host Cell Interaction, UMR 5233 University Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France
    Cell Host Microbe 2:1-2. 2007
  4. pmc A gp41 MPER-specific llama VHH requires a hydrophobic CDR3 for neutralization but not for antigen recognition
    David Lutje Hulsik
    Unit of Virus Host Cell Interactions UVHCI, UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS Pathog 9:e1003202. 2013
  5. pmc Crystal structure and size-dependent neutralization properties of HK20, a human monoclonal antibody binding to the highly conserved heptad repeat 1 of gp41
    Charles Sabin
    Unit of Virus Host Cell Interactions, UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS Pathog 6:e1001195. 2010
  6. pmc Crystal structure of the neutralizing Llama V(HH) D7 and its mode of HIV-1 gp120 interaction
    Andreas Hinz
    Unit of Virus Host Cell Interactions UVHCI, UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS ONE 5:e10482. 2010
  7. pmc Crystal structure of HIV-1 gp41 including both fusion peptide and membrane proximal external regions
    Victor Buzon
    Unit of Virus Host Cell Interactions UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS Pathog 6:e1000880. 2010
  8. doi request reprint A small switch has a large effect on autophagy
    Winfried Weissenhorn
    University Grenoble Alpes, 38000 Grenoble, France CNRS, UVHCI, 38000 Grenoble, France Unit of Virus Host Cell Interactions, University Grenoble Alpes EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France Electronic address
    Structure 22:1-2. 2014
  9. doi request reprint How to get out: ssRNA enveloped viruses and membrane fission
    Winfried Weissenhorn
    Unit of Virus Host Cell Interactions UVHCI, UMI 3265, Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Curr Opin Virol 3:159-67. 2013
  10. ncbi request reprint Structural basis of tetherin function
    Winfried Weissenhorn
    Unit of Virus Host Cell Interactions UVHCI UMI 3265 Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Curr HIV Res 10:298-306. 2012

Collaborators

Detail Information

Publications45

  1. pmc Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p
    Andreas Bracher
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    EMBO J 21:6114-24. 2002
    ..The observed Sly1p-Sed5p interaction mode therefore indicates how SM proteins can stay associated with the assembling fusion machinery in order to participate in late fusion steps...
  2. ncbi request reprint Filovirus assembly and budding
    Bettina Hartlieb
    Institut fur Virologie, Robert Koch str 17, 35037 Marburg, Germany
    Virology 344:64-70. 2006
    ..As a consequence, assembly and budding steps are defined to specific cellular compartments, and the recent progress in understanding how the different components are assembled into stable enveloped virus particles is reviewed...
  3. ncbi request reprint ESCRT-I part II: forming the real ESCRT-I complex
    Winfried Weissenhorn
    Unit for Virus Host Cell Interaction, UMR 5233 University Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France
    Cell Host Microbe 2:1-2. 2007
    ..shed new light on the structure and function of ESCRT-I. Both papers focus on a fourth ESCRT-I component that shapes ESCRT-I into an elongated structure and affects HIV-1 morphogenesis...
  4. pmc A gp41 MPER-specific llama VHH requires a hydrophobic CDR3 for neutralization but not for antigen recognition
    David Lutje Hulsik
    Unit of Virus Host Cell Interactions UVHCI, UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS Pathog 9:e1003202. 2013
    ..Thus bi-2H10 is an anti-MPER antibody generated by immunization that requires hydrophobic CDR3 determinants in addition to epitope recognition for neutralization similar to the mode of neutralization employed by mAbs 2F5 and 4E10...
  5. pmc Crystal structure and size-dependent neutralization properties of HK20, a human monoclonal antibody binding to the highly conserved heptad repeat 1 of gp41
    Charles Sabin
    Unit of Virus Host Cell Interactions, UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS Pathog 6:e1001195. 2010
    ....
  6. pmc Crystal structure of the neutralizing Llama V(HH) D7 and its mode of HIV-1 gp120 interaction
    Andreas Hinz
    Unit of Virus Host Cell Interactions UVHCI, UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS ONE 5:e10482. 2010
    ..Furthermore, our data confirm that the structural plasticity of gp120 can accommodate multiple modes of antibody binding within the CD4 binding site...
  7. pmc Crystal structure of HIV-1 gp41 including both fusion peptide and membrane proximal external regions
    Victor Buzon
    Unit of Virus Host Cell Interactions UMI 3265, Université Joseph Fourier EMBL CNRS, Grenoble, France
    PLoS Pathog 6:e1000880. 2010
    ....
  8. doi request reprint A small switch has a large effect on autophagy
    Winfried Weissenhorn
    University Grenoble Alpes, 38000 Grenoble, France CNRS, UVHCI, 38000 Grenoble, France Unit of Virus Host Cell Interactions, University Grenoble Alpes EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France Electronic address
    Structure 22:1-2. 2014
    ....
  9. doi request reprint How to get out: ssRNA enveloped viruses and membrane fission
    Winfried Weissenhorn
    Unit of Virus Host Cell Interactions UVHCI, UMI 3265, Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Curr Opin Virol 3:159-67. 2013
    ..We relate ESCRT function to budding of single stranded RNA viruses and discuss alternative ways to mediate membrane fission that may govern ESCRT-independent budding...
  10. ncbi request reprint Structural basis of tetherin function
    Winfried Weissenhorn
    Unit of Virus Host Cell Interactions UVHCI UMI 3265 Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Curr HIV Res 10:298-306. 2012
    ..Here we review recent advances in the understanding of the dynamic structural properties of tetherin that provide the basis to physically retain HIV-1 by bridging plasma and virion membranes after completion of budding...
  11. ncbi request reprint Virus membrane fusion
    Winfried Weissenhorn
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    FEBS Lett 581:2150-5. 2007
    ....
  12. pmc A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filaments
    Ricardo Pires
    Unit of Virus Host Cell Interactions UVHCI UMI 3265, Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, Cedex 9, France
    Structure 17:843-56. 2009
    ..Our data suggest that dimeric ALIX represents the active form that interacts with ESCRT-III CHMP4 polymers and functions as a scaffolding protein during membrane remodeling processes...
  13. ncbi request reprint Structural basis for budding by the ESCRT-III factor CHMP3
    Tadeusz Muzioł
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    Dev Cell 10:821-30. 2006
    ..Thus, the final steps in the budding process may include CHMP protein polymerization and lattice formation on membranes by employing different bilayer-recognizing surfaces, a function shared by all CHMP family members...
  14. pmc Structural basis of dynamic glycine receptor clustering by gephyrin
    Maria Sola
    European Molecular Biology Laboratory, Grenoble, France
    EMBO J 23:2510-9. 2004
    ....
  15. pmc Structural basis of HIV-1 tethering to membranes by the BST-2/tetherin ectodomain
    Andreas Hinz
    Unit of Virus Host Cell Interactions UVHCI UMI 3265 Université Joseph Fourier EMBL CNRS, 38042 Grenoble, France
    Cell Host Microbe 7:314-23. 2010
    ..We propose that the irregular coiled coil provides conformational flexibility, ensuring that BST-2/tetherin anchoring both in the plasma membrane and in the newly formed virus membrane is maintained during virus budding...
  16. pmc Structural basis for ESCRT-III CHMP3 recruitment of AMSH
    Julianna Solomons
    Unit of Virus Host Cell Interactions UVHCI UMI 3265 Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz 38042 Grenoble Cedex 9, France
    Structure 19:1149-59. 2011
    ..Our results indicate a tight coupling of ESCRT-III CHMP3 and AMSH functions and provide insight into the regulation of ESCRT-III...
  17. pmc Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei reveals conformational plasticity at its C-terminus
    Andreas Bracher
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    BMC Struct Biol 4:6. 2004
    ..The N-terminal Habc domain fulfills important regulatory functions while the coiled-coil motif, present in all SNAREs, is sufficient for SNARE complex formation, which is thought to drive membrane fusion...
  18. ncbi request reprint Isolation and crystallization of a unique size category of recombinant Rabies virus Nucleoprotein-RNA rings
    Aurélie A V Albertini
    Institut de Virologie Moléculaire et Structurale, FRE 2854 Université Joseph Fourier CNRS, IVMS, c o EMBL, BP 181, 38042 Grenoble Cedex 9, France
    J Struct Biol 158:129-33. 2007
    ..The best diffracting crystals, diffracting up to 6A, contained rings with 11 N-protomers plus an RNA molecule of 99 nucleotides. The diffraction limit was improved to 3.5A by air dehydration prior to flash freezing...
  19. ncbi request reprint Crystal structure of the rabies virus nucleoprotein-RNA complex
    Aurélie A V Albertini
    Institut de Virologie Moléculaire et Structurale, FRE 2854 Université Joseph Fourier CNRS, Boite Postale 181, 38042 Grenoble, France
    Science 313:360-3. 2006
    ....
  20. ncbi request reprint Trimeric membrane-anchored gp41 inhibits HIV membrane fusion
    Oliver Lenz
    European Molecular Biology Laboratory EMBL, 6, rue Jules Horowitz, 38042 Grenoble, France
    J Biol Chem 280:4095-101. 2005
    ..Our data suggest that liposome-anchored gp41ctm exerts its inhibitory action outside of the initial fusion contact site, and its implications for the fusion reaction are discussed...
  21. doi request reprint RNA induced polymerization of the Borna disease virus nucleoprotein
    Miriam Hock
    Unit of Virus Host Cell Interactions UVHCI UMI 3265 Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz 38042 Grenoble Cedex 9, France
    Virology 397:64-72. 2010
    ..Our data indicate that BDV N utilizes similar structural principles for N-RNA and N-P-RNA complex formation as observed for related negative strand RNA viruses...
  22. doi request reprint Characterization of a trimeric MPER containing HIV-1 gp41 antigen
    Andreas Hinz
    Unit for Virus Host Cell Interaction, UMI 3265 UJF EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Virology 390:221-7. 2009
    ..Thus trimerisation of MPER regions does not suffice to induce a potent neutralizing antibody response specific for conserved regions within gp41...
  23. doi request reprint Structure and function of ESCRT-III
    Suman Lata
    Unit for Virus Host Cell Interaction, UMR 5233 UJF EMBL CNRS, 6 rue Jules Horowitz 38042 Grenoble Cedex 9, France
    Biochem Soc Trans 37:156-60. 2009
    ..Progress towards the understanding of the structures and function of ESCRT-III, its activation, its regulation by accessory factors and its role in abscission of membrane enveloped structures in concert with VPS4 are discussed...
  24. pmc CC2D1A is a regulator of ESCRT-III CHMP4B
    Nicolas Martinelli
    Unit of Virus Host Cell Interactions UMI 3265, Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    J Mol Biol 419:75-88. 2012
    ..Thus, CC2D1A might act as a negative regulator of CHMP4B function...
  25. ncbi request reprint The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment
    Estela Pineda-Molina
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, 38042 Grenoble, France
    Traffic 7:1007-16. 2006
    ..Because both Alix and Vps28-CTD can directly recruit ESCRT-III proteins, ESCRT-III assembly coupled to Vps4 action may therefore constitute the minimal budding machinery for EIAV release...
  26. pmc Crystal structure of the C-terminal domain of Ebola virus VP30 reveals a role in transcription and nucleocapsid association
    Bettina Hartlieb
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, 38042 Grenoble, France
    Proc Natl Acad Sci U S A 104:624-9. 2007
    ....
  27. ncbi request reprint Oligomerization and polymerization of the filovirus matrix protein VP40
    Joanna Timmins
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    Virology 312:359-68. 2003
    ..Interestingly, Marburg virus VP40 rings reveal a high tendency to polymerize into rods composed of stacked rings. These results may suggest distinct roles for different oligomeric forms of VP40 in the filovirus life cycle...
  28. pmc Helical structures of ESCRT-III are disassembled by VPS4
    Suman Lata
    Unit for Virus Host Cell Interaction, UMR 5233 UJF Université Joseph Fourier EMBL European Molecular Biology Laboratory CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Science 321:1354-7. 2008
    ..Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4...
  29. ncbi request reprint Structural studies on the Ebola virus matrix protein VP40 indicate that matrix proteins of enveloped RNA viruses are analogues but not homologues
    Joanna Timmins
    European Molecular Biology Laboratory, Grenoble, France
    FEMS Microbiol Lett 233:179-86. 2004
    ..Here, we discuss the structural and functional aspects of teh filovirus matrix protein VP40 and compare them to other known matrix protein structures from vesicular stomatitis virus adn retroviral matrix protein...
  30. pmc ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding
    Grégory Effantin
    Unit of Virus Host Cell Interactions, UMI 3265, Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Cell Microbiol 15:213-26. 2013
    ..Our data thus indicate that the CHMP2A-CHMP3 polymer observed in vitro contributes to HIV-1 budding by assembling on CHMP4B polymers...
  31. pmc Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II
    Amy K Wernimont
    European Molecular Biology Laboratory, Grenoble, France
    BMC Struct Biol 4:10. 2004
    ..In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity...
  32. pmc Crenarchaeal CdvA forms double-helical filaments containing DNA and interacts with ESCRT-III-like CdvB
    Christine Moriscot
    CNRS EMBL UJF, Unit of Virus Host Cell Interactions UVHCI UMI 3265, Grenoble, France
    PLoS ONE 6:e21921. 2011
    ..Here, we report the biochemical and biophysical characterization of the three Cdv proteins from the hyperthermophilic archaeon Metallospherae sedula...
  33. ncbi request reprint The matrix protein VP40 from Ebola virus octamerizes into pore-like structures with specific RNA binding properties
    F Xavier Gomis-Ruth
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, 38042, Grenoble, France
    Structure 11:423-33. 2003
    ..SDS-resistant octameric VP40 is also found in Ebola virus-infected cells, which suggests that VP40 has an additional function in the life cycle of the virus besides promoting virus assembly and budding off the plasma membrane...
  34. pmc Oligomerization of type III secretion proteins PopB and PopD precedes pore formation in Pseudomonas
    Guy Schoehn
    Laboratoire de Virologie Molèculaire Structurale, Grenoble, France
    EMBO J 22:4957-67. 2003
    ..Thus, formation of metastable oligomers precedes membrane association and ring generation in the formation of the Pseudomonas translocon, a mechanism which may be similar for other pathogens that employ type III secretion systems...
  35. ncbi request reprint Crystal structure of the endophilin-A1 BAR domain
    Winfried Weissenhorn
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, 38042 Grenoble, France
    J Mol Biol 351:653-61. 2005
    ..The endophilin-1A BAR domain thus constitutes a new variant of a BAR domain, and it may link endophilin-1A BAR function to calcium regulation of endocytosis...
  36. ncbi request reprint X-ray structure of a neuronal complexin-SNARE complex from squid
    Andreas Bracher
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    J Biol Chem 277:26517-23. 2002
    ..Accordingly, Ca(2+) may initiate membrane fusion by acting directly or indirectly on complexin, thus allowing the conformational transitions of the trans SNARE complex that are thought to drive membrane fusion...
  37. pmc Structural basis for autoinhibition of ESCRT-III CHMP3
    Suman Lata
    Unit for Virus Host Cell Interaction, UMR 5233 UJF EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    J Mol Biol 378:818-27. 2008
    ..Thus, AMSH, including its MIT (microtubule interacting and transport) domain, interacts with ESCRT-III CHMP3 differently from reported Vps4 MIT domain-CHMP protein interactions...
  38. ncbi request reprint Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4
    Joanna Timmins
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    J Mol Biol 326:493-502. 2003
    ..These results suggest that hNedd4 and Tsg101 may play complimentary roles at a late stage of the assembly process, by recruiting cellular factors of two independent pathways to the site of budding at the plasma membrane...
  39. doi request reprint Cryo-electron microscopy three-dimensional structure of the jumbo phage ΦRSL1 infecting the phytopathogen Ralstonia solanacearum
    Grégory Effantin
    Unit of Virus Host Cell Interactions, UMI 3265 UJF EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex, France
    Structure 21:298-305. 2013
    ..Our structural analyses indicate that ϕRSL1 is an unusual member of the Myoviridae that employs conserved protein machines related to different phages and bacteria...
  40. ncbi request reprint Class I and class II viral fusion protein structures reveal similar principles in membrane fusion
    David J Schibli
    European Molecular Biology Laboratory, Grenoble, France
    Mol Membr Biol 21:361-71. 2004
    ..This review will focus on the structures of viral class I and class II fusion proteins and their similarities in facilitating membrane fusion...
  41. pmc Crystal structure of the GABA(A)-receptor-associated protein, GABARAP
    Vassiliy N Bavro
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, BP181, F 38042 Grenoble 9, France
    EMBO Rep 3:183-9. 2002
    ....
  42. ncbi request reprint Essential Ingredients for HIV-1 Budding
    Winfried Weissenhorn
    UVHCI UMI 3265 University Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France
    Cell Host Microbe 9:172-4. 2011
    ..Three papers now begin to demystify its mode of action by showing that HIV-1 requires only the transient recruitment of a surprisingly small subset of ESCRT-III components, whose membrane abscission function depends on VPS4 activity...
  43. pmc VP40 octamers are essential for Ebola virus replication
    Thomas Hoenen
    Institut fur Virologie, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 79:1898-905. 2005
    ..In contrast, no recombinant virus expressing the VP40 Arg134-to-Ala mutation could be recovered. These results suggest that RNA binding of VP40 and therefore octamer formation are essential for the Ebola virus life cycle...
  44. ncbi request reprint An all-atom model of the pore-like structure of hexameric VP40 from Ebola: structural insights into the monomer-hexamer transition
    Tam Luong Nguyen
    Target Structure Based Drug Discovery Group, Developmental Therapeutics Program, SAIC, National Cancer Institute, Frederick, MD 21702, USA
    J Struct Biol 151:30-40. 2005
    ....
  45. pmc Subviral particle release determinants of prototype foamy virus
    Annett Stange
    Institut fur Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Fetscherstr 74, 01307 Dresden, Germany
    J Virol 82:9858-69. 2008
    ..PFV therefore differs from hepatitis B virus in using the same cellular pathway for egress of both virions and SVPs...