Research Topics
| C RiekelSummaryAffiliation: European Synchrotron Radiation Facility Country: France Publications
| Collaborators
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Detail Information
Publications
Protein crystallography microdiffractionChristian Riekel
European Synchrotron Radiation Facility, BP 220, F 38043 Grenoble Cedex, France
Curr Opin Struct Biol 15:556-62. 2005..6 A. Progress in X-ray optical systems and instrumentation will enable the method to be extended to smaller beams and smaller crystal volumes...
Silk fiber assembly studied by synchrotron radiation SAXS/WAXS and Raman spectroscopyAnne Martel
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex, France
J Am Chem Soc 130:17070-4. 2008..The formation of crystalline beta-sheet domains within the fiber is confirmed by wide-angle X-ray scattering. The assembly process resembles the peptide condensation-ordering model proposed for amyloid cross-beta formation...
Aspects of X-ray diffraction on single spider fibersC Riekel
European Synchrotron Radiation Facility, Grenoble, France
Int J Biol Macromol 24:179-86. 1999..The azimuthal spread of the short-range order fraction was about 86 degrees fwhm...
Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granulesC Riekel
European Synchrotron Radiation Facility, BP 220, F 38043 Grenoble Cedex, France
J Synchrotron Radiat 17:743-50. 2010..By careful dose selection during data collection, raster scans with 500 nm step-resolution could be performed for granules immersed in water...
Influence of CO2 on the micro-structural properties of spider dragline silk: X-ray microdiffraction resultsChristian Riekel
European Synchrotron Radiation Facility, BP 220, 38043 Grenoble Cedex, France
Naturwissenschaften 91:30-3. 2004..Long CO(2) exposure reduces the axial alignment of nanofibrils, presumably due to a partial destruction of the amorphous network...
Recent synchrotron radiation microdiffraction experiments on polymer and biopolymer fibersC Riekel
European Synchrotron Radiation Facility, B P 220, 38043, Grenoble Cedex, France
Anal Bioanal Chem 376:594-601. 2003..The possibilities for in situ studies during stretching, extrusion, or indentation will be discussed...
Microstructural homogeneity of support silk spun by Eriophora fuliginea (C.L. Koch) determined by scanning X-ray microdiffractionC Riekel
European Synchrotron Radiation Facility, BP 220, 38043 Grenoble, France
Naturwissenschaften 88:67-72. 2001..A helical model is tentatively proposed to describe this morphology. The central thread has a homogeneous distribution of crystallinity along the macroscopic fibre axis...
Spider silk fibre extrusion: combined wide- and small-angle X-ray microdiffraction experimentsC Riekel
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex, France
Int J Biol Macromol 29:203-10. 2001..Under natural conditions it is the spider's tarsal claws which might serve to squeeze out the water to improve the mechanical properties of the thread during dragline production...
Thermal behavior of Bombyx mori silk: evolution of crystalline parameters, molecular structure, and mechanical propertiesA Martel
European Synchrotron Radiation Facility, Grenoble Cedex, France
Biomacromolecules 8:3548-56. 2007..It is proposed that this is due to the development of cross-linking in the short-range-order chain fraction...
In situ X-ray scattering studies of protein solution droplets drying on micro- and nanopatterned superhydrophobic PMMA surfacesAngelo Accardo
Center of Bio Nanotechnology and Engineering for Medicine, University Magna Græcia, Viale Europa, Catanzaro 88100, Italy
Langmuir 26:15057-64. 2010..Such surfaces represent nearly contact-free sample supports for studies of inorganic and organic solution droplets, which find applications in biochips...
Structural changes of casein micelles in a calcium gradient filmRonald Gebhardt
European Synchrotron Radiation Facility, BP 220, F 38043 Grenoble Cedex 09, France
Macromol Biosci 8:347-54. 2008..Increasing calcium concentration causes a decrease in casein micelle diameter while the size of casein mini-micelles increases and micellar calcium phosphate particles remain unchanged...
Extending the possibilities in phase space analysis of synchrotron radiation x-ray opticsClaudio Ferrero
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex, France
Appl Opt 47:E116-24. 2008..This approach has been implemented into a simple and easy-to-use program toolkit for optical calculations based on the Mathematica software package...
High-pressure potato starch granule gelatinization: synchrotron radiation micro-SAXS/WAXS using a diamond anvil cellR Gebhardt
European Synchrotron Radiation Facility, Grenoble Cedex, France
Biomacromolecules 8:2092-7. 2007..The onset of gelatinization is accompanied by (i) an increase in the average a axis and lamellar periodicity, (ii) the appearance of an equatorial SAXS streak, and (iii) additional short-range order peaks...
Na-cellulose formation in a single cotton fiber studied by synchrotron radiation microdiffractionJ Schoeck
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex, France
Biomacromolecules 8:602-10. 2007..A stitching technique was used to limit radiation damage. Subsequent exposure of the NaOH containing macrodrop to a stream of H2O or HCl microdrops converted part of the Na-cellulose I back into cellulose I...
Recent developments in micro-diffraction on protein crystalsChristian Riekel
European Synchrotron Radiation Facility, BP 220, F-38043 Grenoble, France
J Synchrotron Radiat 11:4-6. 2004..The development of a dedicated microgoniometer in the frame of an EMBL/ESRF collaboration has revealed the potential of micro-diffraction for protein crystallography and is a step in the quest for user friendly instrumentation...
Characterization of the boundary zone of a cast protein drop: fibroin beta-sheet and nanofibril formationRonald Gebhardt
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex, France
Langmuir 25:6307-11. 2009..MicroRaman spectroscopy shows the formation of beta-sheet secondary structure in the boundary zone, which is attributed to a shearing effect due to the retraction of the drop boundary upon evaporation...
In situ muGISAXS: II. Thaumatin crystal growth kineticRonald Gebhardt
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex, France
Biophys J 99:1262-7. 2010....
Amyloid fibril formation from sequences of a natural beta-structured fibrous protein, the adenovirus fiberKaterina Papanikolopoulou
Institut de Biologie Structurale, UMR 5075, CEA-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble, European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
J Biol Chem 280:2481-90. 2005..Structural analysis of these amyloid fibrils suggests that perpendicular stacking of beta-strand repeat units is an underlying common feature of amyloid formation...
Structural evolution of regenerated silk fibroin under shear: combined wide- and small-angle x-ray scattering experiments using synchrotron radiationManfred Rössle
European Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, D 22603 Hamburg, Germany
Biopolymers 74:316-27. 2004..This silk I type material shows up to two low-angle peaks suggesting the presence of water molecules that might be intercalated between hydrogen-bonded sheets...
Comparative architecture of silks, fibrous proteins and their encoding genes in insects and spidersCatherine L Craig
Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USA
Comp Biochem Physiol B Biochem Mol Biol 133:493-507. 2002..Differential regulation of gene expression and selective splicing of the mRNA transcript may allow rapid adaptation of silk functional properties to different physical environments...
Protein crystallography with a micrometre-sized synchrotron-radiation beamRouslan Moukhametzianov
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, England
Acta Crystallogr D Biol Crystallogr 64:158-66. 2008....
Fast intracrystalline hydration of beta-chitin revealed by combined microdrop generation and on-line synchrotron radiation microdiffractionManfred Rössle
European Synchrotron Radiation Facility, B P 220, F 38043 Grenoble Cedex France
Biomacromolecules 4:981-6. 2003..A disordered intermediate phase with variable d-spacing that could be due to a mixture of anhydrous and hydrated beta-chitin layers was also detected...
Atomic structures of amyloid cross-beta spines reveal varied steric zippersMichael R Sawaya
Howard Hughes Medical Institute, UCLA DOE Institute of Genomics and Proteomics, Los Angeles, California 90095 1570, USA
Nature 447:453-7. 2007..Structures of 13 of these microcrystals all reveal steric zippers, but with variations that expand the range of atomic architectures for amyloid-like fibrils and offer an atomic-level hypothesis for the basis of prion strains...
The mechanical properties of hydrated intermediate filaments: insights from hagfish slime threadsDouglas S Fudge
Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
Biophys J 85:2015-27. 2003..X-ray diffraction and congo-red staining indicate that post-yield deformation leads to an irreversible alpha-->beta conformational transition in IFs, which leads to plastic deformation, and may be used by cells as a mechanosensory cue...
Control of crystal size and lattice formation by starmaker in otolith biomineralizationChristian Sollner
Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35, 72076 Tubingen, Germany
Science 302:282-6. 2003..The expression pattern of starmaker, along with the presence of the protein on the growing otolith, suggest that the expression levels of starmaker control the shape of the otoliths...
Structural study of poly(L-lactic acid) spherulitesMassimo Gazzano
Department of Chemistry G Ciamician and ISOF CNR, University of Bologna, Via Selmi 2, 40126 Bologna, Italy
Biomacromolecules 5:553-8. 2004..Concomitantly, a gradual broadening of all X-ray reflections was observed...
Structure of the cross-beta spine of amyloid-like fibrilsRebecca Nelson
Howard Hughes Medical Institute, UCLA DOE Institute for Genomics and Proteomics, Box 951570, UCLA, Los Angeles, California 90095 1570, USA
Nature 435:773-8. 2005..The structure illuminates the stability of amyloid fibrils, their self-seeding characteristic and their tendency to form polymorphic structures...
Some introductory remarks on microbeam diffraction in nanobiosciencesOskar Paris
Department of Biomaterials, Max-Planck-Institute of Colloids and Interfaces, , D-14476 Potsdam-Golm, Germany
J Synchrotron Radiat 12:712. 2005
Morphological and structural aspects of the giant starch granules from Phajus grandifoliusHenri Chanzy
Centre de Recherches sur les Macromolecules Vegetales, ICMG CNRS, BP 53, F 38041 Grenoble Cedex 9, France
J Struct Biol 154:100-10. 2006..The occurrence of these morphological and structural features is tentatively correlated with the mode of growth of these granules...
The structural biology of protein aggregation diseases: Fundamental questions and some answersDavid Eisenberg
Howard Hughes Medical Institute, UCLA DOE Institute of Genomics and Proteomics, Los Angeles, California 90095 1570, USA
Acc Chem Res 39:568-75. 2006....
Mapping fibre orientation in complex-shaped biological systems with micrometre resolution by scanning X-ray microdiffractionRobin Seidel
Department of Biomaterials, Max Plank Institute of Colloids and Interfaces, Research Campus Golm, 14424 Potsdam, Germany
Micron 39:198-205. 2008..The resulting two-dimensional maps of the local fibre orientations - together with the complex shape of the flow sensing system - may be useful for a better understanding of the mechanical optimization of such tissues...
microGISAXS and protein nanotemplate crystallization: methods and instrumentationEugenia Pechkova
Fondazione Elba, Via delle Testuggini snc, I-00134 Rome, Italy
J Synchrotron Radiat 12:713-6. 2005..The nanotemplate experimental setup was used for drops containing growing lysozyme crystals at three different stages of growth...
