Research Topics
| Delphine DebarreSummaryAffiliation: Ecole Polytechnique Country: France Publications
| Collaborators
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Detail Information
Publications
Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopyTomoko Watanabe
Department of Physiology Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
BMC Cell Biol 11:38. 2010..Harmonic generation microscopy (HGM) allows one to image LD without the use of exogenous labels. Adaptive optics can be used to correct aberrations that would otherwise degrade the quality and information content of images...
Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversityD Debarre
Laboratory for Optics and Biosciences, Ecole Polytechnique ParisTech, CNRS, and INSERM, Palaiseau, France
J Microsc 244:136-43. 2011..In order to validate our method, we compare our data to the results obtained with a Shack-Hartmann wavefront sensor, and show that comparable precision is achieved...- Image-based adaptive optics for two-photon microscopyDelphine Debarre
Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, United Kingdom
Opt Lett 34:2495-7. 2009..In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues... 
Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryosDelphine Debarre
Laboratory for Optics and Biosciences, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, Ecole Polytechnique, F 91128 Palaiseau, France
Opt Lett 29:2881-3. 2004..Velocimetric THG imaging provides a quantitative description of the dynamics of internal structures in unstained wild-type and mutant embryos...- Structure sensitivity in third-harmonic generation microscopyDelphine Debarre
Laboratory for Optics and Biosciences, Centre National de la Recherche Scientifique, Ecole Polytechnique, 91128 Palaiseau, France
Opt Lett 30:2134-6. 2005..We illustrate this point by focusing-based contrast modulation in THG images of Drosophila embryos... - Adaptive harmonic generation microscopy of mammalian embryosAlexander Jesacher
1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
Opt Lett 34:3154-6. 2009..Images of live mouse embryos show an improved signal level and resolution... - Multicolor two-photon tissue imaging by wavelength mixingPierre Mahou
Laboratory for Optics and Biosciences, Ecole Polytechnique, Centre National de la Recherche Scientifique UMR 7645 and INSERM U696, Palaiseau, France
Nat Methods 9:815-8. 2012..We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos... - Accuracy of correction in modal sensorless adaptive opticsAurélie Facomprez
Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, 91128 Palaiseau, France
Opt Express 20:2598-612. 2012..Finally, we provide guidelines for implementing an optimal correction algorithm depending on the experimental conditions... - Adaptive optics for structured illumination microscopyDelphine Debarre
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom
Opt Express 16:9290-305. 2008..Aberration corrected imaging is demonstrated using fixed fluorescent specimens. Images are further improved using differential aberration imaging for reduction of background fluorescence... - Imaging lipid bodies in cells and tissues using third-harmonic generation microscopyDelphine Debarre
Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, F 91128 Palaiseau, France
Nat Methods 3:47-53. 2006.... - Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz ratesEdward J Botcherby
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom
Proc Natl Acad Sci U S A 109:2919-24. 2012..We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level... - Dynamic aberration correction for multiharmonic microscopyNicolas Olivier
Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, F 91128 Palaiseau, France
Opt Lett 34:3145-7. 2009..We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue... - Cell lineage reconstruction of early zebrafish embryos using label-free nonlinear microscopyNicolas Olivier
Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, Palaiseau, France
Science 329:967-71. 2010..Progressive amplification of this process is the rule, contrasting with classical descriptions of abrupt changes in the system dynamics... - Advances in multiphoton microscopy for imaging embryosWilly Supatto
Laboratory for Optics and Bioscience, Ecole Polytechnique ParisTech, CNRS, and INSERM U696, Palaiseau, France
Curr Opin Genet Dev 21:538-48. 2011..We illustrate the potentials of these new imaging approaches with a selection of recent applications in developmental biology...