Maxime Dahan

Summary

Country: France

Publications

  1. ncbi request reprint Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking
    Maxime Dahan
    Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Supérieure and Université Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France
    Science 302:442-5. 2003
  2. ncbi request reprint From analog to digital: exploring cell dynamics with single quantum dots
    Maxime Dahan
    Laboratoire Kastler Brossel, Ecole Normale Superieure, 24 rue Lhomond, 75005, Paris, France
    Histochem Cell Biol 125:451-6. 2006
  3. doi request reprint Biochemistry. Zooming into live cells
    Fabien Pinaud
    Laboratoire Kastler Brossel, CNRS UMR8552 Physics and Biology Department, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, 46 rue d Ulm, 75005 Paris, France
    Science 320:187-8. 2008
  4. doi request reprint Quantitative nanoscopy of inhibitory synapses: counting gephyrin molecules and receptor binding sites
    Christian G Specht
    Biologie Cellulaire de la Synapse, Inserm U1024, Institute of Biology, Ecole Normale Supérieure ENS, 46 rue d Ulm, Paris 75005, France
    Neuron 79:308-21. 2013
  5. pmc Velocity, processivity, and individual steps of single myosin V molecules in live cells
    Paolo Pierobon
    Institut Curie, Centre de Recherche, Laboratoire Physico Chimie Curie, Centre National de la Recherche Scientifique, UMR 168, Université Pierre et Marie Curie Paris, Paris, France
    Biophys J 96:4268-75. 2009
  6. ncbi request reprint Tracking individual proteins in living cells using single quantum dot imaging
    Sebastien Courty
    Laboratoire Kastler Brossel, Ecole Normale Supérieure and Université Pierre et Marie Curie, Paris, France
    Methods Enzymol 414:211-28. 2006
  7. pmc Multiple association states between glycine receptors and gephyrin identified by SPT analysis
    Marie Virginie Ehrensperger
    Laboratoire Kastler Brossel, Centre National de la Recherche Scientifique UMR8552, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, 75005 Paris, France
    Biophys J 92:3706-18. 2007
  8. ncbi request reprint Single quantum dot tracking of membrane receptors
    Cedric Bouzigues
    Laboratoire Kastler Brossel, Physics Department, Ecole Normale Superieure, Paris, France
    Methods Mol Biol 374:81-91. 2007
  9. doi request reprint A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides
    Samuel Clarke
    Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie, Ecole Normale Superieure, Universite Pierre et Marie Curie PARIS6, 46 Rue d Ulm 75005 Paris, France
    Nanotechnology 22:175103. 2011
  10. ncbi request reprint Cytoskeleton regulation of glycine receptor number at synapses and diffusion in the plasma membrane
    Cécile Charrier
    Laboratoire de Biologie Cellulaire de la Synapse, Institut National de la Sante et de la Recherche Medicale, Unité 789, Ecole Normale Superieure, 75005 Paris, France
    J Neurosci 26:8502-11. 2006

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking
    Maxime Dahan
    Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Supérieure and Université Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France
    Science 302:442-5. 2003
    ..The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors...
  2. ncbi request reprint From analog to digital: exploring cell dynamics with single quantum dots
    Maxime Dahan
    Laboratoire Kastler Brossel, Ecole Normale Superieure, 24 rue Lhomond, 75005, Paris, France
    Histochem Cell Biol 125:451-6. 2006
    ..Single QD imaging readily offers a powerful tool to investigate the organization in cell membranes. Altogether QDs will contribute to more advanced biological imaging and enable new studies on the dynamics of cellular processes...
  3. doi request reprint Biochemistry. Zooming into live cells
    Fabien Pinaud
    Laboratoire Kastler Brossel, CNRS UMR8552 Physics and Biology Department, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, 46 rue d Ulm, 75005 Paris, France
    Science 320:187-8. 2008
  4. doi request reprint Quantitative nanoscopy of inhibitory synapses: counting gephyrin molecules and receptor binding sites
    Christian G Specht
    Biologie Cellulaire de la Synapse, Inserm U1024, Institute of Biology, Ecole Normale Supérieure ENS, 46 rue d Ulm, Paris 75005, France
    Neuron 79:308-21. 2013
    ..The competition of glycine and GABAA receptor complexes for synaptic binding sites highlights the potential of single-molecule imaging to quantify synaptic plasticity on the nanoscopic scale. ..
  5. pmc Velocity, processivity, and individual steps of single myosin V molecules in live cells
    Paolo Pierobon
    Institut Curie, Centre de Recherche, Laboratoire Physico Chimie Curie, Centre National de la Recherche Scientifique, UMR 168, Université Pierre et Marie Curie Paris, Paris, France
    Biophys J 96:4268-75. 2009
    ..Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells...
  6. ncbi request reprint Tracking individual proteins in living cells using single quantum dot imaging
    Sebastien Courty
    Laboratoire Kastler Brossel, Ecole Normale Supérieure and Université Pierre et Marie Curie, Paris, France
    Methods Enzymol 414:211-28. 2006
    ..This chapter presents methods used for tracking single biomolecules coupled to quantum dots in living cells from labeling procedures to the analysis of the quantum dot motion...
  7. pmc Multiple association states between glycine receptors and gephyrin identified by SPT analysis
    Marie Virginie Ehrensperger
    Laboratoire Kastler Brossel, Centre National de la Recherche Scientifique UMR8552, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, 75005 Paris, France
    Biophys J 92:3706-18. 2007
    ..Within clusters, we identified two subpopulations of GlyR with distinct degrees of stabilization between receptors and scaffolding proteins...
  8. ncbi request reprint Single quantum dot tracking of membrane receptors
    Cedric Bouzigues
    Laboratoire Kastler Brossel, Physics Department, Ecole Normale Superieure, Paris, France
    Methods Mol Biol 374:81-91. 2007
    ..Single QD tracking is nevertheless a general method, suitable to study many transmembrane proteins...
  9. doi request reprint A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides
    Samuel Clarke
    Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie, Ecole Normale Superieure, Universite Pierre et Marie Curie PARIS6, 46 Rue d Ulm 75005 Paris, France
    Nanotechnology 22:175103. 2011
    ..These findings showcase the versatility of the peptides for preparing compact NPs of various compositions and sizes, which are easily functionalized, and suitable for a broad range of biomedical applications...
  10. ncbi request reprint Cytoskeleton regulation of glycine receptor number at synapses and diffusion in the plasma membrane
    Cécile Charrier
    Laboratoire de Biologie Cellulaire de la Synapse, Institut National de la Sante et de la Recherche Medicale, Unité 789, Ecole Normale Superieure, 75005 Paris, France
    J Neurosci 26:8502-11. 2006
    ..Consequently, GlyR number at synapses may be rapidly modulated by the cytoskeleton through the regulation of lateral diffusion in the plasma membrane and of receptor stabilization at synapses...
  11. pmc Amplification and temporal filtering during gradient sensing by nerve growth cones probed with a microfluidic assay
    Mathieu Morel
    Laboratoire Kastler Brossel, Centre National de la Recherche Scientifique, Département de Physique and Institut de Biologie de l Ecole normale supérieure, Universite Pierre et Marie Curie, Paris, France
    Biophys J 103:1648-56. 2012
    ..With computational modeling, we determine that these systems-level properties arise at a molecular level from the saturable occupancy response and the lateral dynamics of GABA(A) receptors...
  12. doi request reprint Concentration landscape generators for shear free dynamic chemical stimulation
    Mathieu Morel
    Laboratoire Kastler Brossel, CNRS UMR8552, Département de Physique et Institut de Biologie, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, 46 rue d Ulm, 75005 Paris, France
    Lab Chip 12:1340-6. 2012
    ....
  13. pmc Asymmetric redistribution of GABA receptors during GABA gradient sensing by nerve growth cones analyzed by single quantum dot imaging
    Cedric Bouzigues
    Laboratoire Kastler Brossel, Centre National de la Recherche Scientifique, Physics Department, Ecole Normale Superieure, and Université Pierre et Marie Curie Paris 6, 24, rue Lhomond, 75005 Paris, France
    Proc Natl Acad Sci U S A 104:11251-6. 2007
    ..Altogether, our results reveal a microtubule-dependent polarized reorganization of chemoreceptors at the cell surface and suggest that this polarization serves as an amplification step in GABA gradient sensing by nerve GCs...
  14. doi request reprint Investigating axonal guidance with microdevice-based approaches
    Isabelle Dupin
    Universite de Bordeaux, Interdisciplinary Institute for Neuroscience, Unité Mixte de Recherche 5297, F 33000 Bordeaux, France, Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, Unité Mixte de Recherche 5297, F 33000 Bordeaux, France, and Laboratoire Physico Chimie, Institut Curie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 168, Universite Pierre et Marie Curie Paris 6, 75248 Paris Cedex 05, France
    J Neurosci 33:17647-55. 2013
    ....
  15. doi request reprint PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking
    Ignacio Izeddin
    Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie de l Ecole Normale Supérieure, Universite Pierre et Marie Curie PARIS6, 46 rue d Ulm 75230 Paris Cedex 05, France
    Opt Express 20:4957-67. 2012
    ....
  16. doi request reprint Microfluidic stickers for cell- and tissue-based assays in microchannels
    Mathieu Morel
    Laboratoire Kastler Brossel, CNRS UMR8552, Physics and Biology Department, Ecole Normale Supérieure and Université Pierre et Marie Curie Paris 6, France
    Lab Chip 9:1011-3. 2009
    ..We demonstrate the simplicity and efficiency of the method with HeLa cells, primary cultured neurons and Drosophila tissues...
  17. pmc Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe
    Ignacio Izeddin
    Laboratoire Kastler Brossel, CNRS UMR 8552, Department of Physics, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, Paris, France
    PLoS ONE 6:e15611. 2011
    ....
  18. pmc Targeting and imaging single biomolecules in living cells by complementation-activated light microscopy with split-fluorescent proteins
    Fabien Pinaud
    Laboratoire Kastler Brossel, Centre National de la Recherche Scientifique Unité de Recherche 8552, Physics Department and Institute of Biology, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, 75005 Paris, France
    Proc Natl Acad Sci U S A 108:E201-10. 2011
    ....
  19. doi request reprint Covalent monofunctionalization of peptide-coated quantum dots for single-molecule assays
    Samuel Clarke
    Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Biologie, Ecole Normale Superieure, Universite Pierre et Marie Curie PARIS6, 46 Rue d Ulm 75005 Paris, France
    Nano Lett 10:2147-54. 2010
    ..We precisely characterize these probes by ensemble and SM techniques and apply them to tracking individual proteins in living cells...
  20. doi request reprint A human mutation in Gabrg2 associated with generalized epilepsy alters the membrane dynamics of GABAA receptors
    Walid Bouthour
    Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Recherche en Santé 839, 75005 Paris, France
    Cereb Cortex 22:1542-53. 2012
    ..Alteration of the membrane diffusion of neurotransmitter receptors therefore represents a new mechanism in human epilepsy...
  21. doi request reprint Mapping the Energy and Diffusion Landscapes of Membrane Proteins at the Cell Surface Using High-Density Single-Molecule Imaging and Bayesian Inference: Application to the Multiscale Dynamics of Glycine Receptors in the Neuronal Membrane
    Jean Baptiste Masson
    Physics of Biological Systems, Pasteur Institute, Paris, France Centre National de la Recherche Scientifique UMR 3525, Paris, France Electronic address
    Biophys J 106:74-83. 2014
    ..Overall, our approach provides a powerful and comprehensive framework with which to analyze biochemical interactions in living cells and to decipher the multiscale dynamics of biomolecules in complex cellular environments. ..
  22. doi request reprint Intra-nuclear mobility and target search mechanisms of transcription factors: a single-molecule perspective on gene expression
    Davide Normanno
    Institut de Biologie de l Ecole Normale Supérieure IBENS, CNRS UMR 8197, Ecole Normale Superieure, 46, rue d Ulm, 75005 Paris, France
    Biochim Biophys Acta 1819:482-93. 2012
    ..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing...
  23. doi request reprint Real-time dynamics of RNA polymerase II clustering in live human cells
    Ibrahim I Cisse
    Functional Imaging of Transcription, CNRS UMR8197, Ecole Normale Superieure, Institut de Biologie de l ENS, IBENS, Paris, 75005 France
    Science 341:664-7. 2013
    ..Our results suggest that transient crowding of enzymes may aid in rate-limiting steps of gene regulation. ..
  24. doi request reprint Probing cellular events, one quantum dot at a time
    Fabien Pinaud
    Laboratoire Kastler Brossel, CNRS Unité de recherche 8552, Physics and Biology Department, Ecole Normale Superieure, Universite Pierre et Marie Curie, Paris 6, Paris, France
    Nat Methods 7:275-85. 2010
    ....
  25. doi request reprint Role of TI-VAMP and CD82 in EGFR cell-surface dynamics and signaling
    Lydia Danglot
    INSERM U950, Membrane Traffic in Neuronal and Epithelial Morphogenesis, Paris, F 75013, France
    J Cell Sci 123:723-35. 2010
    ..These results highlight the role of TI-VAMP in the secretory pathway of a tetraspanin, and support a model in which CD82 allows EGFR entry in microdomains that control its clathrin-dependent endocytosis and signaling...
  26. ncbi request reprint Tracking individual kinesin motors in living cells using single quantum-dot imaging
    Sebastien Courty
    Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Superieure, Universite Pierre et Marie Curie Paris 6, Paris, France
    Nano Lett 6:1491-5. 2006
    ....
  27. pmc Transient directed motions of GABA(A) receptors in growth cones detected by a speed correlation index
    Cedric Bouzigues
    Laboratoire Kastler Brossel, Ecole Normale Superieure, Centre National de la Recherche Scientifique UMR 8552, Paris, France
    Biophys J 92:654-60. 2007
    ..Our observations point to the possibility of a cytoskeleton-dependent redistribution of the sensing molecules in the membrane, which could play a role in the modulation of the cell response to external signals...
  28. ncbi request reprint Imaging the lateral diffusion of membrane molecules with quantum dots
    Hiroko Bannai
    INSERM U789, Biologie Cellulaire de la Synapse N and P, Ecole Normale Superieure Paris, 46, rue d Ulm 75005 Paris, France
    Nat Protoc 1:2628-34. 2006
    ..The experimental procedure described for neurons below takes about 45 min. This technique is applicable to various cultured cells...
  29. ncbi request reprint Single quantum dot tracking based on perceptual grouping using minimal paths in a spatiotemporal volume
    Stephane Bonneau
    CEREMADE, Universite Paris Dauphine, 75775 Paris Cedex 16, France
    IEEE Trans Image Process 14:1384-95. 2005
    ..While developed for tracking QDs, this method can, however, be used with any fluorescent probes...
  30. doi request reprint Interaction between water-soluble peptidic CdSe/ZnS nanocrystals and membranes: formation of hybrid vesicles and condensed lamellar phases
    Aurélien Dif
    Sciences Chimiques de Rennes, CNRS UMR 6226, Universite Rennes 1, 35042 Rennes, France
    J Am Chem Soc 130:8289-96. 2008
    ..In this structure, strong undulations of the bilayer maximize the electrostatic interaction between the QDs and the bilayers, as previously observed in the case of DNA polyelectrolytes interacting with small vesicles...