Daniel Choquet

Summary

Country: France

Publications

  1. ncbi request reprint The role of receptor diffusion in the organization of the postsynaptic membrane
    Daniel Choquet
    Physiologie Cellulaire de la Synapse, UMR 5091 CNRS Université de Bordeaux 2, Bordeaux, France
    Nat Rev Neurosci 4:251-65. 2003
  2. doi request reprint Fast AMPAR trafficking for a high-frequency synaptic transmission
    Daniel Choquet
    Laboratory of cell Physiology of the synapse, CNRS, UMR 5091, Bordeaux, France
    Eur J Neurosci 32:250-60. 2010
  3. pmc Surface mobility of postsynaptic AMPARs tunes synaptic transmission
    Martin Heine
    CNRS, UMR 5091, Universite Bordeaux, Bordeaux, France
    Science 320:201-5. 2008
  4. doi request reprint CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin
    Patricio Opazo
    CNRS UMR 5091, Cellular Physiology of the Synapse, Bordeaux, France
    Neuron 67:239-52. 2010
  5. pmc Endocytic trafficking and recycling maintain a pool of mobile surface AMPA receptors required for synaptic potentiation
    Enrica Maria Petrini
    Laboratoire Physiologie Cellulaire de la Synapse, University of Bordeaux and CNRS, Bordeaux, France
    Neuron 63:92-105. 2009
  6. pmc Activity-independent and subunit-specific recruitment of functional AMPA receptors at neurexin/neuroligin contacts
    Martin Heine
    Centre National de la Recherche Scientifique UMR 5091, Physiologie Cellulaire de la Synapse, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
    Proc Natl Acad Sci U S A 105:20947-52. 2008
  7. doi request reprint Neurexin-neuroligin adhesions capture surface-diffusing AMPA receptors through PSD-95 scaffolds
    Magali Mondin
    Interdisciplinary Institute of Neurosciences, CNRS, UMR 5297, Universite de Bordeaux, 33077 Bordeaux, France
    J Neurosci 31:13500-15. 2011
  8. doi request reprint The excitatory postsynaptic density is a size exclusion diffusion environment
    Marianne L Renner
    Physiologie Cellulaire de la Synapse, CNRS UMR 5091, Universite Bordeaux, Institut Francois Magendie, 146 rue Leo Saignat, 33077 Bordeaux Cedex, France
    Neuropharmacology 56:30-6. 2009
  9. pmc Neurexin/neuroligin interaction kinetics characterized by counting single cell-surface attached quantum dots
    Edouard Saint-Michel
    Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique and University of Bordeaux, Bordeaux, France
    Biophys J 97:480-9. 2009
  10. doi request reprint High-content super-resolution imaging of live cell by uPAINT
    Gregory Giannone
    Interdisciplinary Institute for Neuroscience, Universite de Bordeaux, Bordeaux, France
    Methods Mol Biol 950:95-110. 2013

Collaborators

Detail Information

Publications52

  1. ncbi request reprint The role of receptor diffusion in the organization of the postsynaptic membrane
    Daniel Choquet
    Physiologie Cellulaire de la Synapse, UMR 5091 CNRS Université de Bordeaux 2, Bordeaux, France
    Nat Rev Neurosci 4:251-65. 2003
  2. doi request reprint Fast AMPAR trafficking for a high-frequency synaptic transmission
    Daniel Choquet
    Laboratory of cell Physiology of the synapse, CNRS, UMR 5091, Bordeaux, France
    Eur J Neurosci 32:250-60. 2010
    ..I here review recent results that characterize the activity-dependent properties of AMPAR surface trafficking and their possible links to fast synaptic transmission...
  3. pmc Surface mobility of postsynaptic AMPARs tunes synaptic transmission
    Martin Heine
    CNRS, UMR 5091, Universite Bordeaux, Bordeaux, France
    Science 320:201-5. 2008
    ..Physiological regulation of postsynaptic receptor mobility affects the fidelity of synaptic transmission by shaping the frequency dependence of synaptic responses...
  4. doi request reprint CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin
    Patricio Opazo
    CNRS UMR 5091, Cellular Physiology of the Synapse, Bordeaux, France
    Neuron 67:239-52. 2010
    ..Thus, NMDA-dependent Ca(2+) influx in the post-synapse triggers a CaMKII- and Stargazin-dependent decrease in AMPAR diffusional exchange at synapses that controls synaptic function...
  5. pmc Endocytic trafficking and recycling maintain a pool of mobile surface AMPA receptors required for synaptic potentiation
    Enrica Maria Petrini
    Laboratoire Physiologie Cellulaire de la Synapse, University of Bordeaux and CNRS, Bordeaux, France
    Neuron 63:92-105. 2009
    ..Thus, receptor recycling is crucial for maintaining a mobile population of surface AMPARs that can be delivered to synapses for increases in synaptic strength...
  6. pmc Activity-independent and subunit-specific recruitment of functional AMPA receptors at neurexin/neuroligin contacts
    Martin Heine
    Centre National de la Recherche Scientifique UMR 5091, Physiologie Cellulaire de la Synapse, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
    Proc Natl Acad Sci U S A 105:20947-52. 2008
    ..Such rapid and activity-independent accumulation of functional AMPARs at neurexin-1beta-induced postsynapses points to a new role of AMPARs in synaptogenesis...
  7. doi request reprint Neurexin-neuroligin adhesions capture surface-diffusing AMPA receptors through PSD-95 scaffolds
    Magali Mondin
    Interdisciplinary Institute of Neurosciences, CNRS, UMR 5297, Universite de Bordeaux, 33077 Bordeaux, France
    J Neurosci 31:13500-15. 2011
    ..Together, these data reveal a mechanism by which membrane-diffusing AMPARs can be rapidly trapped at PSD-95 scaffolds assembled at nascent neurexin/neuroligin adhesions, in competition with existing synapses...
  8. doi request reprint The excitatory postsynaptic density is a size exclusion diffusion environment
    Marianne L Renner
    Physiologie Cellulaire de la Synapse, CNRS UMR 5091, Universite Bordeaux, Institut Francois Magendie, 146 rue Leo Saignat, 33077 Bordeaux Cedex, France
    Neuropharmacology 56:30-6. 2009
    ..The structure of the synaptic membrane is thus probably organized as a size exclusion matrix and this controls the rate of exchange of molecules with the extrasynaptic membrane...
  9. pmc Neurexin/neuroligin interaction kinetics characterized by counting single cell-surface attached quantum dots
    Edouard Saint-Michel
    Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique and University of Bordeaux, Bordeaux, France
    Biophys J 97:480-9. 2009
    ..As it stands, this simple technique should be applicable to many types of biological ligand/receptor pairs...
  10. doi request reprint High-content super-resolution imaging of live cell by uPAINT
    Gregory Giannone
    Interdisciplinary Institute for Neuroscience, Universite de Bordeaux, Bordeaux, France
    Methods Mol Biol 950:95-110. 2013
    ..Interestingly, any membrane biomolecule that can be labeled with a fluorescent ligand can be studied, making uPAINT an extremely versatile method...
  11. ncbi request reprint Active surface transport of metabotropic glutamate receptors through binding to microtubules and actin flow
    Arnauld Serge
    Physiologie Cellulaire de la Synapse, CNRS UMR 5091, Institut Francois Magendie, rue Camille Saint Saens, 33077 Bordeaux, Cedex, France
    J Cell Sci 116:5015-22. 2003
    ..This process may play a role in receptor targeting and organization during synapse formation or during glutamate-mediated growth cone chemotaxis...
  12. ncbi request reprint Surface trafficking of neurotransmitter receptor: comparison between single-molecule/quantum dot strategies
    Laurent Groc
    Physiologie Cellulaire de la Synapse, Unité Mixte de Recherche 5091, Centre National de la Recherche Scientifique, Universite Bordeaux 2, 33077 Bordeaux, France
    J Neurosci 27:12433-7. 2007
  13. pmc CaMKII-dependent phosphorylation of GluK5 mediates plasticity of kainate receptors
    Mario Carta
    Universite Bordeaux, Institut interdisciplinaire de neurosciences, UMR 5297, Bordeaux, France
    EMBO J 32:496-510. 2013
    ..We propose that CaMKII-dependent phosphorylation of GluK5 is responsible for synaptic depression by untrapping of KARs from the PSD and increased diffusion away from synaptic sites...
  14. ncbi request reprint The interaction between Stargazin and PSD-95 regulates AMPA receptor surface trafficking
    Cecile Bats
    Physiologie Cellulaire de la Synapse, UMR 5091 CNRS Institut François Magendie, Universite Bordeaux, Bordeaux 33077, France
    Neuron 53:719-34. 2007
    ..These results propose a model in which the Stargazin-PSD-95 interaction plays a key role to trap and transiently stabilize diffusing AMPARs in the postsynaptic density...
  15. pmc Regulation of N-cadherin dynamics at neuronal contacts by ligand binding and cytoskeletal coupling
    Olivier Thoumine
    CNRS, UMR 5091, Institut Magendie de Neurosciences, Universite Bordeaux 2, 33077 Bordeaux, France
    Mol Biol Cell 17:862-75. 2006
    ..Finally, spontaneous neuronal contacts enriched in N-cadherin exhibited similar turnover rates, suggesting that such dynamics of N-cadherin may represent an intrinsic mechanism underlying the plasticity of neuronal adhesions...
  16. ncbi request reprint NMDA receptor surface trafficking and synaptic subunit composition are developmentally regulated by the extracellular matrix protein Reelin
    Laurent Groc
    Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5091, 33077 Bordeaux, France
    J Neurosci 27:10165-75. 2007
    ..Our data reveal a new mode of control of synaptic NMDAR assembly at postnatal hippocampal synapses and an unprecedented role of ECM proteins in regulating glutamate receptor surface diffusion...
  17. pmc Weak effect of membrane diffusion on the rate of receptor accumulation at adhesive contacts
    Olivier Thoumine
    CNRS 5091, Universite Bordeaux 2, Bordeaux, France
    Biophys J 89:L40-2. 2005
    ..The accumulation rate was rather insensitive to the type of receptor, suggesting that the long-range membrane diffusion of immunoglobin cell adhesion molecules is not a limiting step in the initiation of neuronal contacts...
  18. pmc Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density
    Gregory Giannone
    Centre National de la Recherche Scientifique UMR 5091, Cellular Physiology of the Synapse, Bordeaux, France
    Biophys J 99:1303-10. 2010
    ..We show here that the unprecedented large statistics obtained by uPAINT on single cells reveal local diffusion properties of specific proteins, either in distinct membrane compartments of adherent cells or in neuronal synapses...
  19. doi request reprint Measurement and characteristics of neurotransmitter receptor surface trafficking (Review)
    Laurent Groc
    Physiologie Cellulaire de la Synapse, Universite Bordeaux, Bordeaux, France
    Mol Membr Biol 25:344-52. 2008
    ..Because our knowledge on neurotransmitter receptor surface diffusion is fairly recent, open questions and experimental challenges facing the field are highlighted throughout the review...
  20. pmc PSD-95 expression controls L-DOPA dyskinesia through dopamine D1 receptor trafficking
    Gregory Porras
    Universite de Bordeaux, Institut des Maladies Neurodégénératives, Bordeaux, France
    J Clin Invest 122:3977-89. 2012
    ..These findings indicate that altering D1R trafficking via synapse-associated scaffolding proteins may be useful in the treatment of dyskinesia in Parkinson's patients...
  21. doi request reprint [Surface mobility of postsynaptic AMPARs tunes synaptic transmission]
    Daniel Choquet
    UMR CNRS 5091, Universite de Bordeaux, 33077, Bordeaux, France
    Med Sci (Paris) 24:548-50. 2008
  22. doi request reprint A three-step model for the synaptic recruitment of AMPA receptors
    Patricio Opazo
    Physiologie Cellulaire de la Synapse, Universite Bordeaux, Bordeaux, France
    Mol Cell Neurosci 46:1-8. 2011
    ..We will describe how the different trafficking steps are regulated during synaptic plasticity or altered during neurodegenerative diseases such as Alzheimer's...
  23. doi request reprint The stress hormone corticosterone conditions AMPAR surface trafficking and synaptic potentiation
    Laurent Groc
    Physiologie Cellulaire de Synapse, UMR 5091 CNRS, Universite Bordeaux 2, Bordeaux 33077, France
    Nat Neurosci 11:868-70. 2008
    ..Furthermore, corticosterone potentiates the increase of synaptic surface GluR2 contents by a chemical long-term potentiation stimulus, revealing the influence that corticosterone has on AMPAR trafficking...
  24. doi request reprint Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging
    Olivier Thoumine
    CNRS UMR 5091, Institut Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
    Chem Rev 108:1565-87. 2008
  25. ncbi request reprint [Optical control of neuronal network activity]
    Laurent Groc
    Unité Physiologie Cellulaire de la Synapse, UMR 5091 CNRS, Universite Bordeaux 2, Bordeaux 33077, France
    Med Sci (Paris) 23:585-7. 2007
  26. pmc NMDA receptor surface mobility depends on NR2A-2B subunits
    Laurent Groc
    Physiologie Cellulaire de la Synapse, Unité Mixte de Recherche UMR 5091, Centre National de la Recherche Scientifique CNRS, Universite Bordeaux 2, 33077 Bordeaux, France
    Proc Natl Acad Sci U S A 103:18769-74. 2006
    ..This suggests that the switch in synaptic NMDAR subtypes depends on the regulation of the receptor surface trafficking...
  27. pmc Real-time analysis and visualization for single-molecule based super-resolution microscopy
    Adel Kechkar
    University of Bordeaux, Interdisciplinary Institute for Neuroscience, Bordeaux, France CNRS UMR 5297, Bordeaux, France
    PLoS ONE 8:e62918. 2013
    ..Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy...
  28. ncbi request reprint Receptor activation and homer differentially control the lateral mobility of metabotropic glutamate receptor 5 in the neuronal membrane
    Arnauld Serge
    Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5091, Institut Francois Magendie, 33077 Bordeaux, France
    J Neurosci 22:3910-20. 2002
    ..Thus, exchange of receptors between dispersed and clustered states is fast and regulated during physiological processes. These properties may explain certain fast changes in receptor composition observed at postsynaptic densities...
  29. pmc Fast turnover of L1 adhesions in neuronal growth cones involving both surface diffusion and exo/endocytosis of L1 molecules
    Caroline Dequidt
    Unité Mixte de Recherche Centre National de la Recherche Scientifique 5091, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
    Mol Biol Cell 18:3131-43. 2007
    ..Thus, unlike for other molecules such as NrCAM or N-cadherin, diffusion/trapping and exo/endocytosis events cooperate to allow the fast renewal of L1 adhesions...
  30. ncbi request reprint Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors
    Laurent Groc
    UMR 5091 CNRS Université Bordeaux 2, Bordeaux 33077, France
    Nat Neurosci 7:695-6. 2004
    ..Differences in mobility were mainly detected for extrasynaptic AMPARs, suggesting that receptor diffusion between synaptic and extrasynaptic domains is involved in plasticity processes...
  31. ncbi request reprint Synaptic structure and diffusion dynamics of synaptic receptors
    Antoine Triller
    Biologie Cellulaire de la Synapse N and P, INSERM U497, Ecole Normale Superieure, Paris, France
    Biol Cell 95:465-76. 2003
    ....
  32. pmc Direct imaging of lateral movements of AMPA receptors inside synapses
    Catherine Tardin
    Centre de Physique Moléculaire Optique et Hertzienne CNRS UMR 5798 et Université Bordeaux 1, 351 Cours de la Liberation, 33405 Talence, France
    EMBO J 22:4656-65. 2003
    ..Altogether, our data show that rapid exchange of receptors between a synaptic and extra-synaptic localization occurs through regulation of receptor diffusion inside synapses...
  33. ncbi request reprint On the stiffness of the natural actin filament decorated with alexa fluor tropomyosin
    Raffaella Adami
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Via Borsari 46, 44100, Ferrara, Italy
    Biophys Chem 104:469-76. 2003
    ..Only two out of the 10 filaments tested were broken under these conditions, yield strength being 50.5 and 55 pN, respectively...
  34. pmc Dynamics of ligand-induced, Rac1-dependent anchoring of cadherins to the actin cytoskeleton
    Mireille Lambert
    Signalisation et Différenciation Cellulaires dans les Systèmes Nerveux et Musculaire, INSERM U440, Universite Paris 6, Institut du Fer a Moulin, 75005 Paris, France
    J Cell Biol 157:469-79. 2002
    ....
  35. ncbi request reprint Recruitment of the kainate receptor subunit glutamate receptor 6 by cadherin/catenin complexes
    Françoise Coussen
    Centre National de la Recherche Scientifique Unite mixte de recherche 5091, Institut Francois Magendie, Paris 75005, France
    J Neurosci 22:6426-36. 2002
    ..These results suggest an important role for cadherin/catenin complexes in the stabilization of kainate receptors at the synaptic membrane during synapse formation and remodeling...
  36. ncbi request reprint Regulation of AMPA receptor lateral movements
    Aren J Borgdorff
    CNRS UMR 5091, Universite de Bordeaux 2, Bordeaux 33077, France
    Nature 417:649-53. 2002
    ..We suggest that calcium influx prevents AMPA receptors from diffusing, and that lateral receptor diffusion to and from synaptic sites acts in the rapid and controlled regulation of receptor numbers at synapses...
  37. ncbi request reprint Trimers of the fibronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation
    Françoise Coussen
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Sci 115:2581-90. 2002
    ..Beads containing monomer or dimer showed only brief binding and diffusive movements. We conclude that clusters of three integrin-binding ligands are necessary and sufficient for coupling to and translocating with the actin cytoskeleton...
  38. pmc NrCAM coupling to the cytoskeleton depends on multiple protein domains and partitioning into lipid rafts
    Julien Falk
    Neurogenèse et Morphogenèse du Développement à l Adulte, UMR 6156 CNRS, Institut de Biologie du Developpement de Marseille, Marseille, France
    Mol Biol Cell 15:4695-709. 2004
    ....
  39. ncbi request reprint Surface trafficking of receptors between synaptic and extrasynaptic membranes: and yet they do move!
    Antoine Triller
    INSERM UR497, Ecole Normale Superieure, 46 rue d Ulm, Paris F75005, France
    Trends Neurosci 28:133-9. 2005
    ..This review is part of the TINS Synaptic Connectivity series...
  40. ncbi request reprint Multiple routes for glutamate receptor trafficking: surface diffusion and membrane traffic cooperate to bring receptors to synapses
    Laurent Cognet
    Centre de Physique Moleculaire Optique et Hertzienne, CNRS UMR 5798 and Université Bordeaux 1, 351 Cours de la Liberation, 33405 Talence Cedex, France
    Sci STKE 2006:pe13. 2006
    ..Together, these approaches have demonstrated that glutamate receptors diffuse at high rates in the neuronal membrane and suggest a key role for surface diffusion in the regulation of receptor numbers at synapses...
  41. ncbi request reprint Nucleation and growth of cadherin adhesions
    Mireille Lambert
    INSERM, U839, Paris, F 75005, France
    Exp Cell Res 313:4025-40. 2007
    ..Foci experienced collapse at the rear of cadherin adhesions. Based on these results, we present a model for the nucleation, directional growth and shrinkage of cadherin adhesions...
  42. ncbi request reprint Neuroscience: wrestling with SUMO
    Françoise Coussen
    Nature 447:271-2. 2007
  43. ncbi request reprint Extracellular interactions between GluR2 and N-cadherin in spine regulation
    Laura Saglietti
    DTI Dulbecco Telethon Institute, CNR Institute of Neuroscience, Cellular and Molecular Pharmacology, Department of Pharmacology, University of Milan, Italy
    Neuron 54:461-77. 2007
    ..Our data indicate that in hippocampal neurons N-cadherin and GluR2 form a synaptic complex that stimulates presynaptic development and function as well as promoting dendritic spine formation...
  44. pmc Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity
    Michael D Ehlers
    Department of Neurobiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 54:447-60. 2007
    ..Thus, local activity restricts GluR1 mobility on a submicron scale, defining an input-specific mechanism for regulating AMPA receptor composition and abundance...
  45. ncbi request reprint Characterization of SynCAM surface trafficking using a SynCAM derived ligand with high homophilic binding affinity
    Christelle Breillat
    UMR CNRS 5091, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
    Biochem Biophys Res Commun 359:655-9. 2007
    ..Both immobile and highly mobile SynCAM were found. Thus, SynCAM-Fc behaves as a high affinity ligand that can be used to study the function of SynCAM at the neuronal membrane...
  46. pmc Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cells
    David Lasne
    Centre de Physique Moleculaire Optique et Hertzienne, CNRS UMR 5798 and Université Bordeaux 1, Talence Cedex, France
    Biophys J 91:4598-604. 2006
    ..Single Nanoparticle Photothermal Tracking has the unique potential to record arbitrary long trajectory of membrane proteins using nonfluorescent nanometer-sized labels...
  47. ncbi request reprint AMPA and NMDA glutamate receptor trafficking: multiple roads for reaching and leaving the synapse
    Laurent Groc
    UMR 5091 CNRS Université de Bordeaux 2 Physiologie Cellulaire de la Synapse, Institut Francois Magendie, rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    Cell Tissue Res 326:423-38. 2006
    ..We focus on new findings that shed light on the regulation of receptor cycling events and surface trafficking and the way that this might reshape our thinking about the specific regulation of receptor accumulation at synapses...
  48. doi request reprint A molecular clutch between the actin flow and N-cadherin adhesions drives growth cone migration
    Lucie Bard
    Unité Mixte de Recherche 5091, Centre National de la Recherche Scientifique, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
    J Neurosci 28:5879-90. 2008
    ..Together, these data support a direct transmission of actin-based traction forces to N-cadherin adhesions, through catenin partners, driving growth cone advance and neurite extension...
  49. ncbi request reprint Novel diode-pumped infrared tunable laser system for multi-photon microscopy
    Nelly Deguil
    Amplitude Systemes, Domaine du Haut Carre, 33405 Talence, France
    Microsc Res Tech 63:23-6. 2004
    ..This source is easily tuned from 950 to 1200 nm, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10-nm spectral width...
  50. doi request reprint Robust single-molecule approach for counting autofluorescent proteins
    Laurent Cognet
    Universite Bordeaux, Centre de Physique Moleculaire Optique et Hertzienne, Centre National de la Recherche Scientifique, 351 Cours de la Liberation, 33405 Talence, France
    J Biomed Opt 13:031216. 2008
    ..We applied this methodology to the quantification of small purified citrine multimers...
  51. ncbi request reprint Effects of chemical modification, tropomyosin, and myosin subfragment 1 on the yield strength and critical concentration of F-actin
    Raffaella Adami
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Via Borsari 46, 44100 Ferrara, Italy
    Biochemistry 41:5907-12. 2002
    ..45 to 40 MPa. Decoration of tetramethylrhodamine F-actin with both tropomyosin and myosin subfragment 1, at the 1:1 molar ratio with the actin monomer, produces filaments with an yield strength of 22.3 +/- 0.48 pN...
  52. doi request reprint New concepts in synaptic biology derived from single-molecule imaging
    Antoine Triller
    INSERM UR497, Ecole Normale Superieure, Biologie Cellulaire de la Synapse N and P, 46 rue d Ulm, 75005 Paris, France
    Neuron 59:359-74. 2008
    ..In this primer, we will review the different approaches used to track single molecules in live neurons, compare them to bulk measurements, and discuss the different concepts that have emerged from their application to synaptic biology...