Genomes and Genes
- The dynamic synapseDaniel Choquet
Interdisciplinary Institute for Neurosciences, CNRS, UMR 5297, 146 rue Leo Saignat, 33077 Bordeaux, France IINS, University of Bordeaux, Bordeaux, France Electronic address
Neuron 80:691-703. 2013..We will discuss here the different conceptual and methodological advances that have led to a rethinking of the synapse as an organelle whose function is tightly linked to its dynamic organization. ..
- Fast AMPAR trafficking for a high-frequency synaptic transmissionDaniel Choquet
Laboratory of cell Physiology of the synapse, CNRS, UMR 5091, Bordeaux, France
Eur J Neurosci 32:250-60. 2010..I here review recent results that characterize the activity-dependent properties of AMPAR surface trafficking and their possible links to fast synaptic transmission...
- The role of receptor diffusion in the organization of the postsynaptic membraneDaniel Choquet
Physiologie Cellulaire de la Synapse, UMR 5091 CNRS Université de Bordeaux 2, Bordeaux, France
Nat Rev Neurosci 4:251-65. 2003
- Super-resolution imaging reveals that AMPA receptors inside synapses are dynamically organized in nanodomains regulated by PSD95Deepak Nair
University of Bordeaux, Interdisciplinary Institute for Neuroscience, and Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5297, F 33000 Bordeaux, France
J Neurosci 33:13204-24. 2013..Furthermore, our results indicate that glutamatergic synaptic transmission is controlled by the nanometer-scale regulation of the size of these highly concentrated nanodomains. ..
- Unified quantitative model of AMPA receptor trafficking at synapsesKatalin Czöndör
Interdisciplinary Institute for Neuroscience, University of Bordeaux, F 33000 Bordeaux, France
Proc Natl Acad Sci U S A 109:3522-7. 2012..Thus, by discriminating the relative contributions of AMPAR diffusion, trapping, and recycling events on spatial and temporal bases, this model provides unique insights on the dynamic regulation of synaptic strength...
- Endocytic trafficking and recycling maintain a pool of mobile surface AMPA receptors required for synaptic potentiationEnrica Maria Petrini
Laboratoire Physiologie Cellulaire de la Synapse, University of Bordeaux and CNRS, Bordeaux, France
Neuron 63:92-105. 2009..Thus, receptor recycling is crucial for maintaining a mobile population of surface AMPARs that can be delivered to synapses for increases in synaptic strength...
- Surface mobility of postsynaptic AMPARs tunes synaptic transmissionMartin Heine
CNRS, UMR 5091, Universite Bordeaux, Bordeaux, France
Science 320:201-5. 2008..Physiological regulation of postsynaptic receptor mobility affects the fidelity of synaptic transmission by shaping the frequency dependence of synaptic responses...
- Neurexin-neuroligin adhesions capture surface-diffusing AMPA receptors through PSD-95 scaffoldsMagali Mondin
Interdisciplinary Institute of Neurosciences, CNRS, UMR 5297, Universite de Bordeaux, 33077 Bordeaux, France
J Neurosci 31:13500-15. 2011..Together, these data reveal a mechanism by which membrane-diffusing AMPARs can be rapidly trapped at PSD-95 scaffolds assembled at nascent neurexin/neuroligin adhesions, in competition with existing synapses...
- Dynamic and specific interaction between synaptic NR2-NMDA receptor and PDZ proteinsLucie Bard
Laboratory for Cellular Physiology of the Synapse, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5091, 33077 Bordeaux, France
Proc Natl Acad Sci U S A 107:19561-6. 2010..These data reveal an unexpected role of the NR2 subunit divalent arrangement in providing specific anchoring within synapses, highlighting the need to study such dynamic interactions in native conditions...
- CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazinPatricio Opazo
CNRS UMR 5091, Cellular Physiology of the Synapse, Bordeaux, France
Neuron 67:239-52. 2010..Thus, NMDA-dependent Ca(2+) influx in the post-synapse triggers a CaMKII- and Stargazin-dependent decrease in AMPAR diffusional exchange at synapses that controls synaptic function...
- Activity-independent and subunit-specific recruitment of functional AMPA receptors at neurexin/neuroligin contactsMartin Heine
Centre National de la Recherche Scientifique UMR 5091, Physiologie Cellulaire de la Synapse, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
Proc Natl Acad Sci U S A 105:20947-52. 2008..Such rapid and activity-independent accumulation of functional AMPARs at neurexin-1beta-induced postsynapses points to a new role of AMPARs in synaptogenesis...
- Neurexin-1β binding to neuroligin-1 triggers the preferential recruitment of PSD-95 versus gephyrin through tyrosine phosphorylation of neuroligin-1Gregory Giannone
University Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F 33000 Bordeaux, France
Cell Rep 3:1996-2007. 2013..We propose that ligand-induced changes in the Nlg1 phosphotyrosine level control the balance between excitatory and inhibitory scaffold assembly during synapse formation and stabilization. ..
- Fast turnover of L1 adhesions in neuronal growth cones involving both surface diffusion and exo/endocytosis of L1 moleculesCaroline Dequidt
Unité Mixte de Recherche Centre National de la Recherche Scientifique 5091, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
Mol Biol Cell 18:3131-43. 2007..Thus, unlike for other molecules such as NrCAM or N-cadherin, diffusion/trapping and exo/endocytosis events cooperate to allow the fast renewal of L1 adhesions...
- Neurexin/neuroligin interaction kinetics characterized by counting single cell-surface attached quantum dotsEdouard Saint-Michel
Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique and University of Bordeaux, Bordeaux, France
Biophys J 97:480-9. 2009..As it stands, this simple technique should be applicable to many types of biological ligand/receptor pairs...
- The excitatory postsynaptic density is a size exclusion diffusion environmentMarianne L Renner
Physiologie Cellulaire de la Synapse, CNRS UMR 5091, Universite Bordeaux, Institut Francois Magendie, 146 rue Leo Saignat, 33077 Bordeaux Cedex, France
Neuropharmacology 56:30-6. 2009..The structure of the synaptic membrane is thus probably organized as a size exclusion matrix and this controls the rate of exchange of molecules with the extrasynaptic membrane...
- High-content super-resolution imaging of live cell by uPAINTGregory Giannone
Interdisciplinary Institute for Neuroscience, Universite de Bordeaux, Bordeaux, France
Methods Mol Biol 950:95-110. 2013..Interestingly, any membrane biomolecule that can be labeled with a fluorescent ligand can be studied, making uPAINT an extremely versatile method...
- A molecular clutch between the actin flow and N-cadherin adhesions drives growth cone migrationLucie Bard
Unité Mixte de Recherche 5091, Centre National de la Recherche Scientifique, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
J Neurosci 28:5879-90. 2008..Together, these data support a direct transmission of actin-based traction forces to N-cadherin adhesions, through catenin partners, driving growth cone advance and neurite extension...
- Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high densityGregory Giannone
Centre National de la Recherche Scientifique UMR 5091, Cellular Physiology of the Synapse, Bordeaux, France
Biophys J 99:1303-10. 2010..We show here that the unprecedented large statistics obtained by uPAINT on single cells reveal local diffusion properties of specific proteins, either in distinct membrane compartments of adherent cells or in neuronal synapses...
- Active surface transport of metabotropic glutamate receptors through binding to microtubules and actin flowArnauld Serge
Physiologie Cellulaire de la Synapse, CNRS UMR 5091, Institut Francois Magendie, rue Camille Saint Saens, 33077 Bordeaux, Cedex, France
J Cell Sci 116:5015-22. 2003..This process may play a role in receptor targeting and organization during synapse formation or during glutamate-mediated growth cone chemotaxis...
- Surface trafficking of neurotransmitter receptor: comparison between single-molecule/quantum dot strategiesLaurent Groc
Physiologie Cellulaire de la Synapse, Unité Mixte de Recherche 5091, Centre National de la Recherche Scientifique, Universite Bordeaux 2, 33077 Bordeaux, France
J Neurosci 27:12433-7. 2007
- Lengthening of the Stargazin Cytoplasmic Tail Increases Synaptic Transmission by Promoting Interaction to Deeper Domains of PSD-95Anne Sophie Hafner
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, 33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, 33000 Bordeaux, France
Neuron 86:475-89. 2015..Our study reveals that the concerted orientation of the stargazin C-tail and PSD-95 is a major determinant of synaptic strength. ..
- Probing the dynamics of protein-protein interactions at neuronal contacts by optical imagingOlivier Thoumine
CNRS UMR 5091, Institut Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
Chem Rev 108:1565-87. 2008
- CaMKII-dependent phosphorylation of GluK5 mediates plasticity of kainate receptorsMario Carta
Universite Bordeaux, Institut interdisciplinaire de neurosciences, UMR 5297, Bordeaux, France
EMBO J 32:496-510. 2013..We propose that CaMKII-dependent phosphorylation of GluK5 is responsible for synaptic depression by untrapping of KARs from the PSD and increased diffusion away from synaptic sites...
- The interaction between Stargazin and PSD-95 regulates AMPA receptor surface traffickingCecile Bats
Physiologie Cellulaire de la Synapse, UMR 5091 CNRS Institut François Magendie, Universite Bordeaux, Bordeaux 33077, France
Neuron 53:719-34. 2007..These results propose a model in which the Stargazin-PSD-95 interaction plays a key role to trap and transiently stabilize diffusing AMPARs in the postsynaptic density...
- Regulation of N-cadherin dynamics at neuronal contacts by ligand binding and cytoskeletal couplingOlivier Thoumine
CNRS, UMR 5091, Institut Magendie de Neurosciences, Universite Bordeaux 2, 33077 Bordeaux, France
Mol Biol Cell 17:862-75. 2006..Finally, spontaneous neuronal contacts enriched in N-cadherin exhibited similar turnover rates, suggesting that such dynamics of N-cadherin may represent an intrinsic mechanism underlying the plasticity of neuronal adhesions...
- Characterization of SynCAM surface trafficking using a SynCAM derived ligand with high homophilic binding affinityChristelle Breillat
UMR CNRS 5091, Institut Francois Magendie, Universite Bordeaux 2, 33077 Bordeaux, France
Biochem Biophys Res Commun 359:655-9. 2007..Both immobile and highly mobile SynCAM were found. Thus, SynCAM-Fc behaves as a high affinity ligand that can be used to study the function of SynCAM at the neuronal membrane...
- Weak effect of membrane diffusion on the rate of receptor accumulation at adhesive contactsOlivier Thoumine
CNRS 5091, Universite Bordeaux 2, Bordeaux, France
Biophys J 89:L40-2. 2005..The accumulation rate was rather insensitive to the type of receptor, suggesting that the long-range membrane diffusion of immunoglobin cell adhesion molecules is not a limiting step in the initiation of neuronal contacts...
- NMDA receptor surface trafficking and synaptic subunit composition are developmentally regulated by the extracellular matrix protein ReelinLaurent Groc
Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5091, 33077 Bordeaux, France
J Neurosci 27:10165-75. 2007..Our data reveal a new mode of control of synaptic NMDAR assembly at postnatal hippocampal synapses and an unprecedented role of ECM proteins in regulating glutamate receptor surface diffusion...
- Regulation of AMPA receptor surface diffusion by PSD-95 slotsPatricio Opazo
University of Bordeaux, Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, F 33000 Bordeaux, France
Curr Opin Neurobiol 22:453-60. 2012..We will overview the molecular basis of this critical interaction, its activity-dependent regulation and its potential contribution to LTP...
- A three-step model for the synaptic recruitment of AMPA receptorsPatricio Opazo
Physiologie Cellulaire de la Synapse, Universite Bordeaux, Bordeaux, France
Mol Cell Neurosci 46:1-8. 2011..We will describe how the different trafficking steps are regulated during synaptic plasticity or altered during neurodegenerative diseases such as Alzheimer's...
- Measurement and characteristics of neurotransmitter receptor surface trafficking (Review)Laurent Groc
Physiologie Cellulaire de la Synapse, Universite Bordeaux, Bordeaux, France
Mol Membr Biol 25:344-52. 2008..Because our knowledge on neurotransmitter receptor surface diffusion is fairly recent, open questions and experimental challenges facing the field are highlighted throughout the review...
- Real-time analysis and visualization for single-molecule based super-resolution microscopyAdel Kechkar
Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France
PLoS ONE 8:e62918. 2013..Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy...
- Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesionsOlivier Rossier
Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F 33000 Bordeaux, France
Nat Cell Biol 14:1057-67. 2012..Thus, differential transmission of actin motion to fibronectin occurs through specific integrins within FAs...
- Altered surface trafficking of presynaptic cannabinoid type 1 receptor in and out synaptic terminals parallels receptor desensitizationLenka Mikasova
Institut National de la Santé et de la Recherche Médicale U862 Laboratory Physiopathology of Synaptic Plasticity, Neurocentre Magendie, 146 rue Leo Saignat, 33077 Bordeaux, France
Proc Natl Acad Sci U S A 105:18596-601. 2008..The results suggest that decrease of mobility may be one of the core mechanisms underlying the desensitization of CB1R, the most abundant G protein-coupled receptor in the brain...
- PSD-95 expression controls L-DOPA dyskinesia through dopamine D1 receptor traffickingGregory Porras
Universite de Bordeaux, Institut des Maladies Neurodégénératives, Bordeaux, France
J Clin Invest 122:3977-89. 2012..These findings indicate that altering D1R trafficking via synapse-associated scaffolding proteins may be useful in the treatment of dyskinesia in Parkinson's patients...
- [Surface mobility of postsynaptic AMPARs tunes synaptic transmission]Daniel Choquet
UMR CNRS 5091, Universite de Bordeaux, 33077, Bordeaux, France
Med Sci (Paris) 24:548-50. 2008
- The stress hormone corticosterone conditions AMPAR surface trafficking and synaptic potentiationLaurent Groc
Physiologie Cellulaire de Synapse, UMR 5091 CNRS, Universite Bordeaux 2, Bordeaux 33077, France
Nat Neurosci 11:868-70. 2008..Furthermore, corticosterone potentiates the increase of synaptic surface GluR2 contents by a chemical long-term potentiation stimulus, revealing the influence that corticosterone has on AMPAR trafficking...
- [Optical control of neuronal network activity]Laurent Groc
Unité Physiologie Cellulaire de la Synapse, UMR 5091 CNRS, Universite Bordeaux 2, Bordeaux 33077, France
Med Sci (Paris) 23:585-7. 2007
- miR-92a regulates expression of synaptic GluA1-containing AMPA receptors during homeostatic scalingMathieu Letellier
1 University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France 2 CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France 3
Nat Neurosci 17:1040-2. 2014..Deleting the seed region in GluA1 or overexpressing miR-92a blocked homeostatic scaling, indicating that miR-92a regulates the translation and synaptic incorporation of new GluA1-containing AMPA receptors. ..
- Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusionAnaël Chazeau
Interdisciplinary Institute for Neuroscience, University Bordeaux UMR 5297, Bordeaux, France CNRS, Interdisciplinary Institute for NeuroScience UMR 5297, Bordeaux, France
EMBO J 33:2745-64. 2014..Thus, the specific localization of branched F-actin regulators in spines might be reorganized during spine morphological remodeling often associated with synaptic plasticity. ..
- NMDA receptor surface mobility depends on NR2A-2B subunitsLaurent Groc
Physiologie Cellulaire de la Synapse, Unité Mixte de Recherche UMR 5091, Centre National de la Recherche Scientifique CNRS, Universite Bordeaux 2, 33077 Bordeaux, France
Proc Natl Acad Sci U S A 103:18769-74. 2006..This suggests that the switch in synaptic NMDAR subtypes depends on the regulation of the receptor surface trafficking...
- Glutamate-induced AMPA receptor desensitization increases their mobility and modulates short-term plasticity through unbinding from StargazinAudrey Constals
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F 33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F 33000 Bordeaux, France
Neuron 85:787-803. 2015....
- Receptor activation and homer differentially control the lateral mobility of metabotropic glutamate receptor 5 in the neuronal membraneArnauld Serge
Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5091, Institut Francois Magendie, 33077 Bordeaux, France
J Neurosci 22:3910-20. 2002..Thus, exchange of receptors between dispersed and clustered states is fast and regulated during physiological processes. These properties may explain certain fast changes in receptor composition observed at postsynaptic densities...
- Synaptic structure and diffusion dynamics of synaptic receptorsAntoine Triller
Biologie Cellulaire de la Synapse N and P, INSERM U497, Ecole Normale Superieure, Paris, France
Biol Cell 95:465-76. 2003....
- On the stiffness of the natural actin filament decorated with alexa fluor tropomyosinRaffaella Adami
Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Via Borsari 46, 44100, Ferrara, Italy
Biophys Chem 104:469-76. 2003..Only two out of the 10 filaments tested were broken under these conditions, yield strength being 50.5 and 55 pN, respectively...
- Direct imaging of lateral movements of AMPA receptors inside synapsesCatherine Tardin
Centre de Physique Moléculaire Optique et Hertzienne CNRS UMR 5798 et Université Bordeaux 1, 351 Cours de la Liberation, 33405 Talence, France
EMBO J 22:4656-65. 2003..Altogether, our data show that rapid exchange of receptors between a synaptic and extra-synaptic localization occurs through regulation of receptor diffusion inside synapses...
- Recruitment of the kainate receptor subunit glutamate receptor 6 by cadherin/catenin complexesFrançoise Coussen
Centre National de la Recherche Scientifique Unite mixte de recherche 5091, Institut Francois Magendie, Paris 75005, France
J Neurosci 22:6426-36. 2002..These results suggest an important role for cadherin/catenin complexes in the stabilization of kainate receptors at the synaptic membrane during synapse formation and remodeling...
- Regulation of AMPA receptor lateral movementsAren J Borgdorff
CNRS UMR 5091, Universite de Bordeaux 2, Bordeaux 33077, France
Nature 417:649-53. 2002..We suggest that calcium influx prevents AMPA receptors from diffusing, and that lateral receptor diffusion to and from synaptic sites acts in the rapid and controlled regulation of receptor numbers at synapses...
- Trimers of the fibronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocationFrançoise Coussen
Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
J Cell Sci 115:2581-90. 2002..Beads containing monomer or dimer showed only brief binding and diffusive movements. We conclude that clusters of three integrin-binding ligands are necessary and sufficient for coupling to and translocating with the actin cytoskeleton...
- Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptorsLaurent Groc
UMR 5091 CNRS Université Bordeaux 2, Bordeaux 33077, France
Nat Neurosci 7:695-6. 2004..Differences in mobility were mainly detected for extrasynaptic AMPARs, suggesting that receptor diffusion between synaptic and extrasynaptic domains is involved in plasticity processes...
- NrCAM coupling to the cytoskeleton depends on multiple protein domains and partitioning into lipid raftsJulien Falk
Neurogenèse et Morphogenèse du Développement à l Adulte, UMR 6156 CNRS, Institut de Biologie du Developpement de Marseille, Marseille, France
Mol Biol Cell 15:4695-709. 2004....
- Nucleation and growth of cadherin adhesionsMireille Lambert
INSERM, U839, Paris, F 75005, France
Exp Cell Res 313:4025-40. 2007..Foci experienced collapse at the rear of cadherin adhesions. Based on these results, we present a model for the nucleation, directional growth and shrinkage of cadherin adhesions...
- Neuroscience: wrestling with SUMOFrançoise Coussen
Nature 447:271-2. 2007
- Extracellular interactions between GluR2 and N-cadherin in spine regulationLaura Saglietti
DTI Dulbecco Telethon Institute, CNR Institute of Neuroscience, Cellular and Molecular Pharmacology, Department of Pharmacology, University of Milan, Italy
Neuron 54:461-77. 2007..Our data indicate that in hippocampal neurons N-cadherin and GluR2 form a synaptic complex that stimulates presynaptic development and function as well as promoting dendritic spine formation...
- Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activityMichael D Ehlers
Department of Neurobiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
Neuron 54:447-60. 2007..Thus, local activity restricts GluR1 mobility on a submicron scale, defining an input-specific mechanism for regulating AMPA receptor composition and abundance...
- Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cellsDavid Lasne
Centre de Physique Moleculaire Optique et Hertzienne, CNRS UMR 5798 and Université Bordeaux 1, Talence Cedex, France
Biophys J 91:4598-604. 2006..Single Nanoparticle Photothermal Tracking has the unique potential to record arbitrary long trajectory of membrane proteins using nonfluorescent nanometer-sized labels...
- AMPA and NMDA glutamate receptor trafficking: multiple roads for reaching and leaving the synapseLaurent Groc
UMR 5091 CNRS Université de Bordeaux 2 Physiologie Cellulaire de la Synapse, Institut Francois Magendie, rue Camille Saint Saens, 33077 Bordeaux Cedex, France
Cell Tissue Res 326:423-38. 2006..We focus on new findings that shed light on the regulation of receptor cycling events and surface trafficking and the way that this might reshape our thinking about the specific regulation of receptor accumulation at synapses...
- Multiple routes for glutamate receptor trafficking: surface diffusion and membrane traffic cooperate to bring receptors to synapsesLaurent Cognet
Centre de Physique Moleculaire Optique et Hertzienne, CNRS UMR 5798 and Université Bordeaux 1, 351 Cours de la Liberation, 33405 Talence Cedex, France
Sci STKE 2006:pe13. 2006..Together, these approaches have demonstrated that glutamate receptors diffuse at high rates in the neuronal membrane and suggest a key role for surface diffusion in the regulation of receptor numbers at synapses...
- Dynamics of ligand-induced, Rac1-dependent anchoring of cadherins to the actin cytoskeletonMireille Lambert
Signalisation et Différenciation Cellulaires dans les Systèmes Nerveux et Musculaire, INSERM U440, Universite Paris 6, Institut du Fer a Moulin, 75005 Paris, France
J Cell Biol 157:469-79. 2002....
- Surface trafficking of receptors between synaptic and extrasynaptic membranes: and yet they do move!Antoine Triller
INSERM UR497, Ecole Normale Superieure, 46 rue d Ulm, Paris F75005, France
Trends Neurosci 28:133-9. 2005..This review is part of the TINS Synaptic Connectivity series...
- New concepts in synaptic biology derived from single-molecule imagingAntoine Triller
INSERM UR497, Ecole Normale Superieure, Biologie Cellulaire de la Synapse N and P, 46 rue d Ulm, 75005 Paris, France
Neuron 59:359-74. 2008..In this primer, we will review the different approaches used to track single molecules in live neurons, compare them to bulk measurements, and discuss the different concepts that have emerged from their application to synaptic biology...
- Robust single-molecule approach for counting autofluorescent proteinsLaurent Cognet
Universite Bordeaux, Centre de Physique Moleculaire Optique et Hertzienne, Centre National de la Recherche Scientifique, 351 Cours de la Liberation, 33405 Talence, France
J Biomed Opt 13:031216. 2008..We applied this methodology to the quantification of small purified citrine multimers...
- Novel diode-pumped infrared tunable laser system for multi-photon microscopyNelly Deguil
Amplitude Systemes, Domaine du Haut Carre, 33405 Talence, France
Microsc Res Tech 63:23-6. 2004..This source is easily tuned from 950 to 1200 nm, without any laser adjustment, and delivers sub-300 femtosecond pulses with a 10-nm spectral width...
- Effects of chemical modification, tropomyosin, and myosin subfragment 1 on the yield strength and critical concentration of F-actinRaffaella Adami
Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Via Borsari 46, 44100 Ferrara, Italy
Biochemistry 41:5907-12. 2002..45 to 40 MPa. Decoration of tetramethylrhodamine F-actin with both tropomyosin and myosin subfragment 1, at the 1:1 molar ratio with the actin monomer, produces filaments with an yield strength of 22.3 +/- 0.48 pN...