Christophe Leger

Summary

Affiliation: Centre National de la Recherche Scientifique
Country: France

Publications

  1. ncbi request reprint Inhibition and aerobic inactivation kinetics of Desulfovibrio fructosovorans NiFe hydrogenase studied by protein film voltammetry
    Christophe Leger
    Unite de Bioenergetique et Ingenierie des Proteines, Institut de Biologie Structurale et Microbiologie, CNRS UPR9036 et Université de Provence, 31, Chemin Joseph Aiguier, 13402, Marseille, France
    J Am Chem Soc 126:12162-72. 2004
  2. ncbi request reprint Electron flow in multicenter enzymes: theory, applications, and consequences on the natural design of redox chains
    Christophe Leger
    Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036, CNRS, IBSM and Université de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 128:180-7. 2006
  3. doi request reprint Direct electrochemistry of redox enzymes as a tool for mechanistic studies
    Christophe Leger
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM, and Universite de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Chem Rev 108:2379-438. 2008
  4. doi request reprint Original design of an oxygen-tolerant [NiFe] hydrogenase: major effect of a valine-to-cysteine mutation near the active site
    Pierre Pol Liebgott
    CNRS, Laboratoire de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la Méditerranée, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 133:986-97. 2011
  5. doi request reprint Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase
    Pierre Pol Liebgott
    Centre National de la Recherche Scientifique, Unité Propre de Recherche 9036, Unite de Bioenergetique et Ingenierie des Proteines, Institut Fédératif de Recherche 88, Institut de Microbiologie de la Mediterranee, Marseille, France
    Nat Chem Biol 6:63-70. 2010
  6. ncbi request reprint Changing the ligation of the distal [4Fe4S] cluster in NiFe hydrogenase impairs inter- and intramolecular electron transfers
    Sebastien Dementin
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM and Université de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 128:5209-18. 2006
  7. doi request reprint Hydrogen-activating enzymes: activity does not correlate with oxygen sensitivity
    Carole Baffert
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, Universite de Provence, Marseille, France
    Angew Chem Int Ed Engl 47:2052-4. 2008
  8. doi request reprint Steady-state catalytic wave-shapes for 2-electron reversible electrocatalysts and enzymes
    Vincent Fourmond
    CNRS, Aix Marseille Univ, BIP UMR 7281, IMM FR 3479, 31 Chemin J Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 135:3926-38. 2013
  9. doi request reprint O2-independent formation of the inactive states of NiFe hydrogenase
    Abbas Abou Hamdan
    Aix Marseille Universite, Centre National de la Recherche Scientifique CNRS, Laboratoire de Bioenergetique et Ingenierie des Proteines, Unité Mixte de Recherche 7281, Institut de Microbiologie de la Mediterranee, Marseille, France
    Nat Chem Biol 9:15-7. 2013
  10. pmc Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants
    Abbas Abou Hamdan
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Centre National de la Recherche Scientifique, Aix Marseille Universite, Institut de Microbiologie de la Mediterranee, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 109:19916-21. 2012

Collaborators

Detail Information

Publications28

  1. ncbi request reprint Inhibition and aerobic inactivation kinetics of Desulfovibrio fructosovorans NiFe hydrogenase studied by protein film voltammetry
    Christophe Leger
    Unite de Bioenergetique et Ingenierie des Proteines, Institut de Biologie Structurale et Microbiologie, CNRS UPR9036 et Université de Provence, 31, Chemin Joseph Aiguier, 13402, Marseille, France
    J Am Chem Soc 126:12162-72. 2004
    ..This experimental approach could be used to study the reaction of other multicentered metalloenzymes with their gaseous substrates or inhibitors...
  2. ncbi request reprint Electron flow in multicenter enzymes: theory, applications, and consequences on the natural design of redox chains
    Christophe Leger
    Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036, CNRS, IBSM and Université de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 128:180-7. 2006
    ..This suggests an alternative to the idea that redox chains are optimized in terms of speed: evolutionary pressure may have resulted in slowing down intramolecular ET in order to tune the enzyme's "operating potential"...
  3. doi request reprint Direct electrochemistry of redox enzymes as a tool for mechanistic studies
    Christophe Leger
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM, and Universite de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Chem Rev 108:2379-438. 2008
  4. doi request reprint Original design of an oxygen-tolerant [NiFe] hydrogenase: major effect of a valine-to-cysteine mutation near the active site
    Pierre Pol Liebgott
    CNRS, Laboratoire de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la Méditerranée, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 133:986-97. 2011
    ..The electrochemical behavior of the V74C mutant toward O(2) is intermediate between that of the WT enzyme from D. fructosovorans and the oxygen-tolerant MBH from Aquifex aeolicus...
  5. doi request reprint Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase
    Pierre Pol Liebgott
    Centre National de la Recherche Scientifique, Unité Propre de Recherche 9036, Unite de Bioenergetique et Ingenierie des Proteines, Institut Fédératif de Recherche 88, Institut de Microbiologie de la Mediterranee, Marseille, France
    Nat Chem Biol 6:63-70. 2010
    ..We quantitatively define the relations between diffusion, the Michaelis constant for H2 and rates of inhibition, and we demonstrate that certain enzymes are slowly inactivated by O2 because access to the active site is slow...
  6. ncbi request reprint Changing the ligation of the distal [4Fe4S] cluster in NiFe hydrogenase impairs inter- and intramolecular electron transfers
    Sebastien Dementin
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM and Université de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 128:5209-18. 2006
    ..This demonstrates that although [4Fe4S] clusters are robust chemical constructs, the direct protein ligands play an essential role in imparting their ability to transfer electrons...
  7. doi request reprint Hydrogen-activating enzymes: activity does not correlate with oxygen sensitivity
    Carole Baffert
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, Universite de Provence, Marseille, France
    Angew Chem Int Ed Engl 47:2052-4. 2008
  8. doi request reprint Steady-state catalytic wave-shapes for 2-electron reversible electrocatalysts and enzymes
    Vincent Fourmond
    CNRS, Aix Marseille Univ, BIP UMR 7281, IMM FR 3479, 31 Chemin J Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 135:3926-38. 2013
    ..The considerations herein will help the interpretation of electrochemical data previously obtained with various other bidirectional oxidoreductases, and, possibly, synthetic inorganic catalysts...
  9. doi request reprint O2-independent formation of the inactive states of NiFe hydrogenase
    Abbas Abou Hamdan
    Aix Marseille Universite, Centre National de la Recherche Scientifique CNRS, Laboratoire de Bioenergetique et Ingenierie des Proteines, Unité Mixte de Recherche 7281, Institut de Microbiologie de la Mediterranee, Marseille, France
    Nat Chem Biol 9:15-7. 2013
    ..Our findings offer new ways toward the understanding of O(2) inactivation and O(2) tolerance in NiFe hydrogenases...
  10. pmc Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants
    Abbas Abou Hamdan
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Centre National de la Recherche Scientifique, Aix Marseille Universite, Institut de Microbiologie de la Mediterranee, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 109:19916-21. 2012
    ....
  11. doi request reprint Dependence of catalytic activity on driving force in solution assays and protein film voltammetry: insights from the comparison of nitrate reductase mutants
    Vincent Fourmond
    Centre National de la Recherche Scientifique, UPR 9036, Unite de Bioenergetique et Ingenierie des Proteines, Institut de Microbiologie de la Mediterranee, and Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochemistry 49:2424-32. 2010
    ....
  12. doi request reprint Understanding and tuning the catalytic bias of hydrogenase
    Abbas Abou Hamdan
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Institut de Microbiologie de la Mediterranee, CNRS and Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 134:8368-71. 2012
    ..We evidence a novel strategy for tuning the catalytic bias of an oxidoreductase, which consists in modulating the rate of a step that is limiting only in one direction of the reaction, without modifying the properties of the active site...
  13. doi request reprint Rates of intra- and intermolecular electron transfers in hydrogenase deduced from steady-state activity measurements
    Sebastien Dementin
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 133:10211-21. 2011
    ..This establishes that FeS-to-FeS rate constants are extremely sensitive to the nature and coordination of the centers...
  14. doi request reprint Major Mo(V) EPR signature of Rhodobacter sphaeroides periplasmic nitrate reductase arising from a dead-end species that activates upon reduction. Relation to other molybdoenzymes from the DMSO reductase family
    Vincent Fourmond
    Unite de Bioenergetique et Ingenierie des Proteines, IBSM, UPR 9036, CNRS, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    J Phys Chem B 112:15478-86. 2008
    ..This could be applied to other enzymes for which the question arises as to the catalytic relevance of certain long-lived, spectroscopically characterized species...
  15. ncbi request reprint In Rhodobacter sphaeroides respiratory nitrate reductase, the kinetics of substrate binding favors intramolecular electron transfer
    Bettina Frangioni
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, 31 Chemin J Aiguier, 13402 Marseille, France
    J Am Chem Soc 126:1328-9. 2004
    ..Importantly, comparison is made with all Mo enzymes for which PFV data are available, and we emphasize general features of the energetics of the catalytic cycles in enzymes of the DMSO reductase family...
  16. doi request reprint Covalent attachment of FeFe hydrogenases to carbon electrodes for direct electron transfer
    Carole Baffert
    CNRS, Aix Marseille Universite, BIP UMR, IMM FR, France
    Anal Chem 84:7999-8005. 2012
    ..We also show that covalent attachment has no effect on the catalytic properties of the enzyme, which means that this strategy can also used be for electrochemical studies of the catalytic mechanism...
  17. doi request reprint Reassessing the strategies for trapping catalytic intermediates during nitrate reductase turnover
    Vincent Fourmond
    Unite de Bioenergetique et Ingenierie des Proteines, Institut de Microbiologie de la Mediterranee, Centre National de la Recherche Scientifique, UPR 9036, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Phys Chem B 114:3341-7. 2010
    ....
  18. doi request reprint Introduction of methionines in the gas channel makes [NiFe] hydrogenase aero-tolerant
    Sebastien Dementin
    CNRS, Bioenergetique et Ingenierie des Proteines, IMM, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 131:10156-64. 2009
    ..These results show for the first time that it is possible to improve O(2)-tolerance of [NiFe] hydrogenases, making possible the development of biohydrogen production systems...
  19. pmc Experimental approaches to kinetics of gas diffusion in hydrogenase
    Fanny Leroux
    Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, Unité Propre de Recherche 9036, Unite de Bioenergetique et Ingenierie des Proteines, Marseille, France
    Proc Natl Acad Sci U S A 105:11188-93. 2008
    ..Our results demonstrate the necessity of measuring diffusion rates and emphasize the role of side-chain dynamics in determining these...
  20. doi request reprint Correcting for electrocatalyst desorption and inactivation in chronoamperometry experiments
    Vincent Fourmond
    Unite de Bioenergetique et Ingenierie des Proteines, IMM, UPR 9036, CNRS, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Anal Chem 81:2962-8. 2009
    ..This method also applies to experiments where electron transfer is mediated, rather than direct, providing the current is proportional to the time-dependent concentration of catalyst...
  21. doi request reprint CO disrupts the reduced H-cluster of FeFe hydrogenase. A combined DFT and protein film voltammetry study
    Carole Baffert
    Bioenergetics and Engineering of Proteins, CNRS, UPR 9036, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 133:2096-9. 2011
    ..Like in the case of oxygen inhibition, the reaction with CO engages the entire H-cluster, rather than only the Fe(2) subsite...
  22. doi request reprint The mechanism of inhibition by H2 of H2-evolution by hydrogenases
    Vincent Fourmond
    CNRS, Aix Marseille Universite, BIP UMR 7281, 13009, Marseille, France
    Chem Commun (Camb) 49:6840-2. 2013
    ....
  23. doi request reprint "Two-step" chronoamperometric method for studying the anaerobic inactivation of an oxygen tolerant NiFe hydrogenase
    Vincent Fourmond
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, UPR 9036, Institut de Biologie de la Mediterranee and Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 132:4848-57. 2010
    ..aeolicus . In particular, the activation and inactivation reactions cannot follow the same reaction pathway...
  24. ncbi request reprint Effects of slow substrate binding and release in redox enzymes: theory and application to periplasmic nitrate reductase
    Patrick Bertrand
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM, France
    J Phys Chem B 111:10300-11. 2007
    ....
  25. doi request reprint Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion
    Julien G J Jacques
    Aix Marseille Universite, CNRS, BIP UMR 7281, 31 Chemin J Aiguier, F 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1837:277-86. 2014
    ..The mechanism implies both the cyclization of the pyran ring and the reduction of the oxidized pterin to give the competent tricyclic tetrahydropyranopterin form. ..
  26. doi request reprint SOAS: a free program to analyze electrochemical data and other one-dimensional signals
    Vincent Fourmond
    Unite de Bioenergetique et Ingenierie des Proteines, UPR 9036, CNRS, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Bioelectrochemistry 76:141-7. 2009
    ..This free program is available by download from the Internet, and can be installed on computers running any flavor of Unix or Linux, most easily on MacOS X...
  27. ncbi request reprint Access to the active site of periplasmic nitrate reductase: insights from site-directed mutagenesis and zinc inhibition studies
    Sebastien Dementin
    Laboratoire de Bioenergetique Cellulaire, CEA Cadarache, DSV IBEB SBVME, 13108 St Paul lez Durance Cedex, France
    Biochemistry 46:9713-21. 2007
    ..This residue is not conserved in the DMSO reductase superfamily while it is conserved in the periplasmic nitrate reductase family. Zinc inhibition is therefore likely to be specific and restricted to periplasmic nitrate reductases...
  28. ncbi request reprint Characterization of two 2[4Fe4S] ferredoxins from Clostridium acetobutylicum
    Olivier Guerrini
    UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, Toulouse F 31400, France
    Curr Microbiol 56:261-7. 2008
    ..The physiological function of CAC3527 is discussed...