Research Topics
| Eric CascalesSummaryAffiliation: Centre National de la Recherche Scientifique Country: France Publications
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Detail Information
Publications
The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wallMarie Stéphanie Aschtgen
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Universite de la Mediterranee Aix Marseille II, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Mol Microbiol 75:886-99. 2010..Using in vivo and in vitro binding assays, we showed that this motif anchors the SciZ protein to the cell wall and is required for T6SS function...
The type VI secretion toolkitEric Cascales
Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, Centre National de la Recherche Scientifique, Marseille, France
EMBO Rep 9:735-41. 2008..This review provides an overview of our current knowledge about type VI secretion, focusing on gene regulation, components of the secretion machine, substrate secretion and the cellular consequences for the host cell...- Colicin biologyEric Cascales
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Microbiol Mol Biol Rev 71:158-229. 2007..The mechanisms of inhibition by specific and cognate immunity proteins are presented. Finally, the use of colicins as laboratory or biotechnological tools and their mode of evolution are discussed... - Mapping the interactions between escherichia coli tol subunits: rotation of the TolR transmembrane helixXiang Y Z Zhang
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 13402 Marseille, France
J Biol Chem 284:4275-82. 2009..TolR anchors may form molecular gears that oscillate in response to proton motive force to regulate channel activity... - Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coliAurélie Barnéoud-Arnoulet
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR 9027, Marseille, France
J Bacteriol 192:5934-42. 2010..For the first time, we also identified an interaction between the TolQ protein and a colicin translocation domain... 
Movements of the TolR C-terminal domain depend on TolQR ionizable key residues and regulate activity of the Tol complexEmilie L Goemaere
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Biol Chem 282:17749-57. 2007..We monitored these structural changes by cysteine accessibility experiments and showed that the conformation of this domain is responsive to the proton-motive force and on the presence of critical residues of the ion pathway...- Structural biology of type VI secretion systemsEric Cascales
CNRS, Laboratoire d Ingenierie des Systemes Macromoleculaires, UMR 7255, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Philos Trans R Soc Lond B Biol Sci 367:1102-11. 2012..In this review, we summarize recent structural information on individual T6SS components emphasizing the fact that T6SSs are composite systems, adapting subunits from various origins... - Mapping the interactions between Escherichia coli TolQ transmembrane segmentsXiang Y Z Zhang
Laboratoire d Ingénierie des Systèmes Macromoléculaires UPR9027, CNRS, Aix Marseille Universite, Marseille, France
J Biol Chem 286:11756-64. 2011.... - An epigenetic switch involving overlapping fur and DNA methylation optimizes expression of a type VI secretion gene clusterYannick R Brunet
Laboratoire d Ingenierie des Systemes Macromoleculaires, CNRS UPR 9027, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, Marseille, France
PLoS Genet 7:e1002205. 2011..A model is proposed in which the sci1 promoter is regulated by iron availability, adenine methylation, and DNA replication... - Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovarCatarina Felisberto-Rodrigues
Aix Marseille Universite, Architecture et Fonction des Macromolecules Biologiques, Campus de Luminy, Marseille, France
PLoS Pathog 7:e1002386. 2011..Collectively, our results provide an improved understanding of T6SS assembly and encourage structure-aided drug design of novel antimicrobials targeting T6SS... - Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteinsChristophe S Bernard
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 193:2158-67. 2011..Using in vitro binding experiments and in vivo reporter fusion assays, we showed that the expression of these clusters is dependent on both σ(54) and bEBPs... - Deletion analyses of the peptidoglycan-associated lipoprotein Pal reveals three independent binding sequences including a TolA boxEric Cascales
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Mol Microbiol 51:873-85. 2004..Furthermore, a conserved polypeptide sequence located downstream of the peptidoglycan binding motif of Pal was required for the TolA-Pal interaction and for the maintenance of outer membrane stability... - Tol-dependent macromolecule import through the Escherichia coli cell envelope requires the presence of an exposed TolA binding motifStéphanie Pommier
Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 187:7526-34. 2005..Furthermore, in vivo and in vitro coprecipitation analyses demonstrated that colicin A and g3p N-terminal domains compete for binding to TolA... - Anchoring the type VI secretion system to the peptidoglycan: TssL, TagL, TagP... what else?Marie Stéphanie Aschtgen
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Centre National de la Recherche Scientifique CNRS, Aix Marseille Universite, Marseille, France
Virulence 1:535-40. 2010..Herein, we discuss these results, examine whether PG-binding motifs are found within other T6SS subunits and express hypotheses regarding the role of PG-binding motifs in type VI secretion... - SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coliMarie Stéphanie Aschtgen
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 190:7523-31. 2008..Together, these results support a model in which SciN is an outer membrane lipoprotein exposed in the periplasm and essential for the Sci-1 apparatus function... - Mutational analyses define helix organization and key residues of a bacterial membrane energy-transducing complexEmilie L Goemaere
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Mol Biol 366:1424-36. 2007..We discuss a model for the function of related bacterial molecular motors... - Analysis of the Escherichia coli Tol-Pal and TonB systems by periplasmic production of Tol, TonB, colicin, or phage capsid soluble domainsEmmanuelle Bouveret
LISM, CNRS, 31, Chemin Joseph Aiguier, 13402, Marseille, Cedex 20, France
Biochimie 84:413-21. 2002..This technique can be used to detect new interactions, to characterize them biochemically and to map them or to induce tol or tonB phenotypes to study the functions of these two complexes... - HxcQ liposecretin is self-piloted to the outer membrane by its N-terminal lipid anchorVéronique Viarre
Laboratoire d Ingénierie des Systèmes Macromoléculaires UPR 9027, CNRS, Institut de Microbiologie de la Mediterranee, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Biol Chem 284:33815-23. 2009..Altogether, our results reveal an original and unprecedented pathway for secretin transport to the OM... - The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidCMarie Stéphanie Aschtgen
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Université CNRS UMR 7255, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
Microbiologyopen 1:71-82. 2012..We further show that this transmembrane segment is essential for the function of the protein and its proper insertion in the inner membrane is dependent upon YidC and modulated by the Hsp70 homologue DnaK... - Nooks and crannies in type VI secretion regulationChristophe S Bernard
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 192:3850-60. 2010.... - Pal lipoprotein of Escherichia coli plays a major role in outer membrane integrityEric Cascales
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 184:754-9. 2002..However, Pal was present in larger amounts compared to TolA and TolR proteins. The oligomeric state of Pal was determined and a new interaction between Pal and Lpp was demonstrated... - Imaging type VI secretion-mediated bacterial killingYannick R Brunet
Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, UMR 7255
Cell Rep 3:36-41. 2013..The results support a model in which T6SS dynamics are responsible for T6SS effectors translocation into recipient cells... - Promoter swapping unveils the role of the Citrobacter rodentium CTS1 type VI secretion system in interbacterial competitionErwan Gueguen
Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, Institut de Microbiologie de la Mediterranee, CNRS UMR7255, Aix Marseille University, Marseille, France
Appl Environ Microbiol 79:32-8. 2013..Artificial induction of both promoters demonstrated that the CTS1 T6SS is functional as shown by the Hcp release assay and confers on C. rodentium a growth advantage in antibacterial competition experiments with Escherichia coli...