Frederic Carriere

Summary

Affiliation: Centre National de la Recherche Scientifique
Country: France

Publications

  1. ncbi request reprint Inhibition of gastrointestinal lipolysis by Orlistat during digestion of test meals in healthy volunteers
    F Carriere
    Laboratoire de Lipolyse Enzymatique, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, F 13402 Marseille Cedex 20, France
    Am J Physiol Gastrointest Liver Physiol 281:G16-28. 2001
  2. ncbi request reprint Advantage of expressing the variations in some digestive parameters as a function of gastric emptying instead of time
    F Carriere
    Laboratoire de Lipolyse Enzymatique du CNRS, Institut de Biologie Structurale et Microbiologie, Marseille, France
    Digestion 64:46-53. 2001
  3. ncbi request reprint Does the pancreas really produce much more lipase than required for fat digestion?
    Frederic Carriere
    Laboratory of Enzymology at Interfaces and Physiology of Lipolysis, CNRS UPR 9025 IBSM, Marseille, France
    JOP 6:206-15. 2005
  4. ncbi request reprint Quantitative study of digestive enzyme secretion and gastrointestinal lipolysis in chronic pancreatitis
    Frederic Carriere
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, IBSM CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Clin Gastroenterol Hepatol 3:28-38. 2005
  5. ncbi request reprint The beta 5' loop of the pancreatic lipase C2-like domain plays a critical role in the lipase-lipid interactions
    Henri Chahinian
    Laboratoire de Lipolyse Enzymatique du CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochemistry 41:13725-35. 2002
  6. ncbi request reprint Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells
    Cécilia Eydoux
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, Centre National de la Recherche Scientifique Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20, France
    J Lipid Res 48:1539-49. 2007
  7. doi request reprint Enantioselective inhibition of microbial lipolytic enzymes by nonracemic monocyclic enolphosphonate analogues of cyclophostin
    Vanessa Point
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Med Chem 56:4393-401. 2013
  8. doi request reprint Carica papaya lipase: a naturally immobilized enzyme with interesting biochemical properties
    Slim Abdelkafi
    CNRS Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse UPR9025, Marseille, France
    Plant Foods Hum Nutr 66:34-40. 2011
  9. ncbi request reprint In vitro digestion of the self-emulsifying lipid excipient Labrasol(®) by gastrointestinal lipases and influence of its colloidal structure on lipolysis rate
    Sylvie Fernandez
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
    Pharm Res 30:3077-87. 2013
  10. ncbi request reprint Exploring the specific features of interfacial enzymology based on lipase studies
    Ahmed Aloulou
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, 31 Chemin Joseph Aiguier, 13009 Marseille Cedex 20, France
    Biochim Biophys Acta 1761:995-1013. 2006

Collaborators

Detail Information

Publications58

  1. ncbi request reprint Inhibition of gastrointestinal lipolysis by Orlistat during digestion of test meals in healthy volunteers
    F Carriere
    Laboratoire de Lipolyse Enzymatique, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, F 13402 Marseille Cedex 20, France
    Am J Physiol Gastrointest Liver Physiol 281:G16-28. 2001
    ..As predicted from the previous results, the effects of Orlistat on fat excretion levels were found to be much greater with the solid (40.5--57.4% of ingested fat) than with the liquid (4.2--18.8%) test meal...
  2. ncbi request reprint Advantage of expressing the variations in some digestive parameters as a function of gastric emptying instead of time
    F Carriere
    Laboratoire de Lipolyse Enzymatique du CNRS, Institut de Biologie Structurale et Microbiologie, Marseille, France
    Digestion 64:46-53. 2001
    ..Here, we investigate whether the parametric variability could be reduced by running experiments on a gastric emptying basis rather than on a time basis...
  3. ncbi request reprint Does the pancreas really produce much more lipase than required for fat digestion?
    Frederic Carriere
    Laboratory of Enzymology at Interfaces and Physiology of Lipolysis, CNRS UPR 9025 IBSM, Marseille, France
    JOP 6:206-15. 2005
    ....
  4. ncbi request reprint Quantitative study of digestive enzyme secretion and gastrointestinal lipolysis in chronic pancreatitis
    Frederic Carriere
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, IBSM CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Clin Gastroenterol Hepatol 3:28-38. 2005
    ..This study was designed to quantify pancreatic and extrapancreatic enzyme output, activity, and stability in CP patients vs. healthy volunteers...
  5. ncbi request reprint The beta 5' loop of the pancreatic lipase C2-like domain plays a critical role in the lipase-lipid interactions
    Henri Chahinian
    Laboratoire de Lipolyse Enzymatique du CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochemistry 41:13725-35. 2002
    ..These observations support a major contribution of the beta 5' loop in the interaction of LPL and HPL with their respective substrates...
  6. ncbi request reprint Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells
    Cécilia Eydoux
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, Centre National de la Recherche Scientifique Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20, France
    J Lipid Res 48:1539-49. 2007
    ..The finding that the inhibition of nonproteolyzed rHPLRP2 by tetrahydrolipstatin and diethyl-p-nitrophenyl phosphate does not involve any bile salt requirements suggests that the rHPLRP2 lid adopts an open conformation in aqueous media...
  7. doi request reprint Enantioselective inhibition of microbial lipolytic enzymes by nonracemic monocyclic enolphosphonate analogues of cyclophostin
    Vanessa Point
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Med Chem 56:4393-401. 2013
    ....
  8. doi request reprint Carica papaya lipase: a naturally immobilized enzyme with interesting biochemical properties
    Slim Abdelkafi
    CNRS Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse UPR9025, Marseille, France
    Plant Foods Hum Nutr 66:34-40. 2011
    ..All these properties suggest that CPL may be a good candidate for various biotechnological applications...
  9. ncbi request reprint In vitro digestion of the self-emulsifying lipid excipient Labrasol(®) by gastrointestinal lipases and influence of its colloidal structure on lipolysis rate
    Sylvie Fernandez
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
    Pharm Res 30:3077-87. 2013
    ..It is a mixture of acylglycerols and PEG esters, these compounds being substrates for digestive lipases. The characterization of Labrasol(®) gastrointestinal lipolysis is essential for understanding its mode of action...
  10. ncbi request reprint Exploring the specific features of interfacial enzymology based on lipase studies
    Ahmed Aloulou
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, 31 Chemin Joseph Aiguier, 13009 Marseille Cedex 20, France
    Biochim Biophys Acta 1761:995-1013. 2006
    ....
  11. doi request reprint Lipolysis of the semi-solid self-emulsifying excipient Gelucire 44/14 by digestive lipases
    Sylvie Fernandez
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, Marseille, France
    Biochim Biophys Acta 1781:367-75. 2008
    ..This is the first time the effects of PEG and acyl chain length on the lipolytic activity of digestive lipases on PEG esters have been investigated...
  12. doi request reprint Lid opening and unfolding in human pancreatic lipase at low pH revealed by site-directed spin labeling EPR and FTIR spectroscopy
    Sebastien Ranaldi
    CNRS Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036, Marseille, France
    Biochemistry 48:630-8. 2009
    ..0 in comparison with that observed in the pH range of 3.0-5.0. This finding suggests that the disulfide bridge between the hinges of the lid kept the secondary structure of the lid intact, whereas the HPL was completely unfolded...
  13. ncbi request reprint Partial deletion of beta9 loop in pancreatic lipase-related protein 2 reduces enzyme activity with a larger effect on long acyl chain substrates
    Kaouthar Dridi
    a CNRS Aix Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1831:1293-301. 2013
    ..All these findings indicate that the reduced enzyme turnover observed with GPLRP2-deltabeta9 results from a weaker stabilization of the acyl enzyme intermediate due to a loss of hydrophobic interactions...
  14. doi request reprint Analysis of the discriminative inhibition of mammalian digestive lipases by 3-phenyl substituted 1,3,4-oxadiazol-2(3H)-ones
    Vanessa Point
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de Lipolyse UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Eur J Med Chem 58:452-63. 2012
    ..These inhibitors could be used for a better understanding of individual lipase function as well as for drug development aiming at the regulation of the whole gastrointestinal lipolysis process...
  15. doi request reprint Continuous measurement of galactolipid hydrolysis by pancreatic lipolytic enzymes using the pH-stat technique and a medium chain monogalactosyl diglyceride as substrate
    Sawsan Amara
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1791:983-90. 2009
    ....
  16. ncbi request reprint A comparative study on two fungal lipases from Thermomyces lanuginosus and Yarrowia lipolytica shows the combined effects of detergents and pH on lipase adsorption and activity
    Ahmed Aloulou
    Laboratory of Enzymology at Interfaces and Physiology of Lipolysis, CNRS UPR 9025 IBSM, 31 Chemin Joseph Aiguier, 13009 Marseille, France
    Biochim Biophys Acta 1771:1446-56. 2007
    ....
  17. doi request reprint Identification of a new phospholipase D in Carica papaya latex
    Slim Abdelkafi
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, Marseille, France
    Gene 499:243-9. 2012
    ..05 kDa. From sequence analysis, CpPLD1 was identified as a PLD belonging to the plant phosphatidylcholine phosphatidohydrolase family...
  18. doi request reprint Lipolysis of natural long chain and synthetic medium chain galactolipids by pancreatic lipase-related protein 2
    Sawsan Amara
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1801:508-16. 2010
    ..PLRP2 might be used to produce lipid and free fatty acid fractions enriched in either 16:3 n-3 or 18:3 n-3 fatty acids, both found at high levels in galactolipids...
  19. ncbi request reprint Two cutinase-like proteins secreted by Mycobacterium tuberculosis show very different lipolytic activities reflecting their physiological function
    Mathieu Schue
    Centre National de la Recherche Scientifique, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse UPR 9025, Marseille, France
    FASEB J 24:1893-903. 2010
    ..Our findings open new prospects for investigating the physiological roles of cutinase-like proteins in the lipid metabolism and virulence of M. tuberculosis...
  20. doi request reprint Amplitude of pancreatic lipase lid opening in solution and identification of spin label conformational subensembles by combining continuous wave and pulsed EPR spectroscopy and molecular dynamics
    Sebastien Ranaldi
    CNRS Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036, Institut de Microbiologie de la Mediterranee, Aix Marseille Universités, Marseille, France
    Biochemistry 49:2140-9. 2010
    ....
  21. ncbi request reprint Constitutive expression of human pancreatic lipase-related protein 1 in Pichia pastoris
    Ahmed Aloulou
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025 CNRS Institut de Biologie Structurale et Microbiologie, Marseille, France
    Protein Expr Purif 47:415-21. 2006
    ..5. N-terminal sequencing and mass spectrometry analysis were carried out to monitor the production of the mature protein and to confirm that its signal peptide was properly processed...
  22. ncbi request reprint Use of an inhibitor to identify members of the hormone-sensitive lipase family
    Yassine Ben Ali
    Enzymology at Interfaces and Physiology of Lipolysis, UPR 9025 CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochemistry 45:14183-91. 2006
    ....
  23. pmc MmPPOX inhibits Mycobacterium tuberculosis lipolytic enzymes belonging to the hormone-sensitive lipase family and alters mycobacterial growth
    Vincent Delorme
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse UMR 7282, Marseille, France
    PLoS ONE 7:e46493. 2012
    ..Overall, these findings suggest that the M. tuberculosis HSL family displays important metabolic functions, thus opening the way to further investigations linking the involvement of these enzymes in mycobacterial growth...
  24. doi request reprint In vitro stereoselective hydrolysis of diacylglycerols by hormone-sensitive lipase
    Jorge A Rodriguez
    CNRS Université d Aix Marseille Enzymologie Interfaciale et Physiologie de la Lipolyse UPR 9025, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1801:77-83. 2010
    ..Among the various mammalian lipases tested so far, HSL is the only lipolytic carboxylester hydrolase found to have a pronounced stereospecificity for the sn-3 position of dioleoylglycerol...
  25. ncbi request reprint Characterization of pancreatic lipase-related protein 2 isolated from human pancreatic juice
    Josiane De Caro
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025 CNRS Institut de Biologie Structurale et Microbiologie, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1701:89-99. 2004
    ..These data suggest that the main physiological function of HPLRP2 is the hydrolysis of galactolipids, which are the main lipids present in vegetable food...
  26. ncbi request reprint In vitro gastrointestinal lipolysis of four formulations of piroxicam and cinnarizine with the self emulsifying excipients Labrasol and Gelucire 44/14
    Sylvie Fernandez
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
    Pharm Res 26:1901-10. 2009
    ..Labrasol and Gelucire 44/14 are defined admixtures of acylglycerols and PEG esters which are substrates for digestive lipases...
  27. ncbi request reprint Human pancreatic lipase-related protein 2: tissular localization along the digestive tract and quantification in pancreatic juice using a specific ELISA
    Cécilia Eydoux
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025, CNRS Institut de Biologie Structurale et Microbiologie, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1760:1497-504. 2006
    ..30% (w/w) and 23.96% (w/w) in controls subjects and CCP patients, respectively, and the difference was not significant. The levels of HPL and HPLRP2 are therefore similarly reduced in both healthy patients and CCP patients...
  28. ncbi request reprint Understanding the lipid-digestion processes in the GI tract before designing lipid-based drug-delivery systems
    Jean Claude Bakala N'Goma
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Ther Deliv 3:105-24. 2012
    ..In this review, we present the latest data available about the lipolytic enzymes involved in gastrointestinal lipolysis and suggest tracks for designing physiologically relevant in vitro digestion models...
  29. doi request reprint Occurrence of pancreatic lipase-related protein-2 in various species and its relationship with herbivore diet
    Josiane De Caro
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, Unité Propre de Recherche 9025, France
    Comp Biochem Physiol B Biochem Mol Biol 150:1-9. 2008
    ..The absence of galactolipids in carnivorous animals' diet may explain why no PLRP2s were detected here in the pancreas of these species...
  30. ncbi request reprint Closed and open conformations of the lid domain induce different patterns of human pancreatic lipase antigenicity and immunogenicity
    Hubert Halimi
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025, CNRS Institut de Biologie Structurale et Microbiologie, 31, Chemin JosephBAiguier, 13402, Marseille Cedex 20, France
    Biochim Biophys Acta 1753:247-56. 2005
    ..These antibodies should provide useful tools for monitoring the conformational changes taking place during the opening of the HPL lid domain...
  31. pmc Synthesis and kinetic evaluation of cyclophostin and cyclipostins phosphonate analogs as selective and potent inhibitors of microbial lipases
    Vanessa Point
    CNRS Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Med Chem 55:10204-19. 2012
    ....
  32. doi request reprint Effects of surfactants on lipase structure, activity, and inhibition
    Vincent Delorme
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse, UPR 9025, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
    Pharm Res 28:1831-42. 2011
    ..Here, we review some examples of the various effects of surfactants on lipase structure, activity and inhibition, which show how complex the various equilibria involved in the lipolysis reaction tend to be...
  33. doi request reprint Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex
    Slim Abdelkafi
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, Marseille, France
    Biochim Biophys Acta 1791:1048-56. 2009
    ....
  34. ncbi request reprint Probing the opening of the pancreatic lipase lid using site-directed spin labeling and EPR spectroscopy
    Valérie Belle
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM, Marseille, France
    Biochemistry 46:2205-14. 2007
    ..The opening of the lid was found to be a reversible process. Using various colipase to lipase molar ratios, a correlation between the proportion of the open conformation and the catalytic activity of HPL was observed...
  35. ncbi request reprint Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica
    Ahmed Aloulou
    Laboratory of Enzymology at Interfaces and Physiology of Lipolysis, CNRS UPR 9025 IBSM, 31 Chemin Joseph Aiguier, 13009 Marseille, France
    Biochim Biophys Acta 1771:228-37. 2007
    ..0, in the presence of bile salts. It is therefore a good candidate for use in enzyme replacement therapy as a means of treating pancreatic exocrine insufficiency...
  36. ncbi request reprint Critical evaluation of a specific ELISA and two enzymatic assays of pancreatic lipases in human sera
    Philippe Grandval
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse du CNRS, UPR 9025, Marseille, France
    Pancreatology 4:495-503; discussion 503-4. 2004
    ..The aim of this study was to develop an ELISA for specifically quantifying the classical-HPL level in sera of patients with and without pancreatic disorders...
  37. ncbi request reprint Physiological study of pH stability and sensitivity to pepsin of human gastric lipase
    Eric Ville
    La Timone Hospital, Gastroenterology Department, Marseille, France
    Digestion 65:73-81. 2002
    ..The aim of this study was to exactly determine the pH stability of human gastric lipase (HGL) and to investigate the mechanism underlying the inactivation of HGL which occurs in gastric juice...
  38. doi request reprint Watching intracellular lipolysis in mycobacteria using time lapse fluorescence microscopy
    Rabeb Dhouib
    CNRS, Aix Marseille Université Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse UPR 9025, 31 Chemin Joseph Aiguier, 13402, Marseille, France
    Biochim Biophys Acta 1811:234-41. 2011
    ..It should be particularly useful for studying the effects of chemical inhibitors and activators on cells as well as investigating other metabolic pathways...
  39. doi request reprint Validation of lipolysis product extraction from aqueous/biological samples, separation and quantification by thin-layer chromatography with flame ionization detection analysis using O-cholesteryl ethylene glycol as a new internal standard
    Jean François Cavalier
    CNRS Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Chromatogr A 1216:6543-8. 2009
    ....
  40. ncbi request reprint Cloning and seasonal secretion of the pancreatic lipase-related protein 2 present in goat seminal plasma
    Barbara Sias
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1686:169-80. 2005
    ..The lipase activity level measured in goat seminal plasma, which could reach 1000 U/ml during the breeding season, was one of the highest lipase activity measured in natural sources, including gastric and pancreatic juices...
  41. ncbi request reprint Continuous monitoring of cholesterol oleate hydrolysis by hormone-sensitive lipase and other cholesterol esterases
    Yassine Ben Ali
    Enzymology at Interfaces and Physiology of Lipolysis, Unité Propre de Recherche 9025, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France
    J Lipid Res 46:994-1000. 2005
    ..Thus, HSL could be considered more as a cholesteryl ester hydrolase than as a TAG lipase...
  42. ncbi request reprint Inhibition of phospholipase A1, lipase and galactolipase activities of pancreatic lipase-related protein 2 by methyl arachidonyl fluorophosphonate (MAFP)
    Sawsan Amara
    CNRS Aix Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1821:1379-85. 2012
    ....
  43. pmc Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids
    Karen Côtes
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025 CNRS, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Biochem J 408:417-27. 2007
    ..tuberculosis. Our results identify Rv0183 as the first exported lipolytic enzyme to be characterized in M. tuberculosis and suggest that Rv0183 may be involved in the degradation of the host cell lipids...
  44. ncbi request reprint Comparative study on digestive lipase activities on the self emulsifying excipient Labrasol, medium chain glycerides and PEG esters
    Sylvie Fernandez
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1771:633-40. 2007
    ..These two pancreatic enzymes, together with gastric lipase, are probably the main enzymes involved in the in vivo lipolysis of Labrasol taken orally...
  45. doi request reprint Structure of human pancreatic lipase-related protein 2 with the lid in an open conformation
    Cécilia Eydoux
    CNRS UPR9025 Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, Marseille, France
    Biochemistry 47:9553-64. 2008
    ..Our findings illustrate the essential role of the lid in determining the substrate specificity and the mechanism of action of lipases...
  46. pmc A monoacylglycerol lipase from Mycobacterium smegmatis Involved in bacterial cell interaction
    Rabeb Dhouib
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse UPR 9025, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 192:4776-85. 2010
    ....
  47. ncbi request reprint How gastric lipase, an interfacial enzyme with a Ser-His-Asp catalytic triad, acts optimally at acidic pH
    Henri Chahinian
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, IBSM, Marseille, France
    Biochemistry 45:993-1001. 2006
    ..This specific kinetic feature of interfacial enzymology should be taken into account when studying any soluble enzyme acting on an insoluble substrate...
  48. ncbi request reprint Sensitive assay for hormone-sensitive lipase using NBD-labeled monoacylglycerol to detect low activities in rat adipocytes
    Stefan Petry
    Aventis Pharma Germany, 65926 Frankfurt am Main, Germany
    J Lipid Res 46:603-14. 2005
    ..This convenient assay procedure based on NBD-MAG should facilitate the search for small molecule HSL inhibitors...
  49. ncbi request reprint An interfacial and comparative in vitro study of gastrointestinal lipases and Yarrowia lipolytica LIP2 lipase, a candidate for enzyme replacement therapy
    Anaïs Bénarouche
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochimie 102:145-53. 2014
    ..All these findings support the use of YLLIP2 in enzyme replacement therapy for the treatment of pancreatic exocrine insufficiency, a pathological situation in which an acidification of intestinal contents occurs...
  50. ncbi request reprint In vitro digestion of citric acid esters of mono- and diglycerides (CITREM) and CITREM-containing infant formula/emulsions
    Sawsan Amara
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Food Funct 5:1409-21. 2014
    ..Nevertheless, undigestible water-soluble compounds containing glycerol and citric acid units were identified, indicating that the ester bond between citric acid and glycerol is not fully hydrolyzed throughout the proposed digestion. ..
  51. doi request reprint Partial deletion of β9 loop in pancreatic lipase-related protein 2 reduces enzyme activity with a larger effect on long acyl chain substrates
    Kaouthar Dridi
    CNRS Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1831:1293-301. 2012
    ..All these findings indicate that the reduced enzyme turnover observed with GPLRP2-Δβ9 results from a weaker stabilization of the acyl enzyme intermediate due to a loss of hydrophobic interactions...
  52. doi request reprint Effects of the propeptide of group X secreted phospholipase A(2) on substrate specificity and interfacial activity on phospholipid monolayers
    Vanessa Point
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, Marseille Cedex, France
    Biochimie 95:51-8. 2013
    ..This model shows that the propeptide is located within the interfacial binding site (i-face) and could disrupt both the interfacial binding of the enzyme and the access to the active site by steric hindrance...
  53. ncbi request reprint Human pancreatic lipase-related protein 2 is a galactolipase
    Barbara Sias
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, CNRS UPR 9025, IBSM, Marseille, France
    Biochemistry 43:10138-48. 2004
    ..The physiological role of HPLRP2 suggested by the present results is the digestion of galactolipids, the most abundant lipids occurring in plant cells, and therefore, in the vegetables that are part of the human diet...
  54. doi request reprint Quantitative study of lipase secretion, extracellular lipolysis, and lipid storage in the yeast Yarrowia lipolytica grown in the presence of olive oil: analogies with lipolysis in humans
    Amal Najjar
    Enzymologie Interfaciale et Physiologie de la Lipolyse, UPR 9025, CNRS, Aix Marseille Universite, Marseille, France
    Appl Microbiol Biotechnol 89:1947-62. 2011
    ..The quantitative data obtained here allow for the first time to establish interesting analogies with gastrointestinal and vascular lipolysis in humans...
  55. doi request reprint The molecular mechanism of human hormone-sensitive lipase inhibition by substituted 3-phenyl-5-alkoxy-1,3,4-oxadiazol-2-ones
    Yassine Ben Ali
    Organization and Dynamics of Biological Membranes, UMR 5246 ICBMS, CNRS Universite Claude Bernard Lyon 1, Batiment Raulin, 43, Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Biochimie 94:137-45. 2012
    ..On the basis of this study, a kinetic model is proposed to describe the inhibition of HSL by compound 7600 in the aqueous phase as well as its partial reactivation at the lipid-water interface...
  56. ncbi request reprint Might the kinetic behavior of hormone-sensitive lipase reflect the absence of the lid domain?
    Yassine Ben Ali
    Enzymology at Interfaces and Physiology of Lipolysis, UPR 9025 CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochemistry 43:9298-306. 2004
    ..The 3-D structure of HSL, which still remains to be determined, may therefore lack the lid domain known to exist in various other lipases...
  57. ncbi request reprint Using the reversible inhibition of gastric lipase by Orlistat for investigating simultaneously lipase adsorption and substrate hydrolysis at the lipid-water interface
    Anaïs Bénarouche
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochimie 101:221-31. 2014
    ..This allowed to assess the kcat/KM* ratio for DGL acting on 1,2-dicaprin monolayer, after showing that mixed monolayers containing a low fraction of Orlistat were similar to pure 1,2-dicaprin monolayers...
  58. ncbi request reprint High-level constitutive expression in Pichia pastoris and one-step purification of phospholipase D from cowpea (Vigna unguiculata L. Walp)
    Yassine Ben Ali
    Enzymology at Interfaces and Physiology of Lipolysis, UPR 9025 IBSM, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Protein Expr Purif 51:162-9. 2007
    ..This functional recombinant PLDalpha should provide a valuable tool for performing detailed studies on the molecular characterization of enzymes as well as structural studies...