Yves Bourne

Summary

Affiliation: Centre National de la Recherche Scientifique
Country: France

Publications

  1. ncbi request reprint Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis
    Chris A Tarling
    Department of Chemistry, University of British Columbia, Vancouver V6T 1Z1, Canada
    J Biol Chem 278:47394-9. 2003
  2. ncbi request reprint Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding
    Yves Bourne
    Ingénierie des Protéines, CNRS FRE 2738, Institut Federatif de Recherche Jean Roche, Université dela Méditerranée, Faculté d eMédecine Secteur Nord, F 13916 Marseille Cedex 20, France
    J Biol Chem 281:29256-67. 2006
  3. pmc Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger
    Isabelle Benoit
    UMR 1163 INRA Université de Provence de Biotechnologie des Champignons Filamenteux, IFR IBAIM, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France
    Appl Environ Microbiol 73:5624-32. 2007
  4. pmc Structural insights into the catalytic mechanism of bacterial guanosine-diphospho-D-mannose pyrophosphorylase and its regulation by divalent ions
    Marie Cécile Pelissier
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS, Universite Aix Marseille, F 13288 Marseille, France
    J Biol Chem 285:27468-76. 2010
  5. ncbi request reprint Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectins
    Y Bourne
    AFMB CNRS, Marseille, France
    Structure 7:1473-82. 1999
  6. ncbi request reprint Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteins
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Structure 12:677-87. 2004
  7. pmc Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, Marseille, France
    EMBO J 24:1512-22. 2005
  8. pmc Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, Marseille, France
    EMBO J 22:1-12. 2003
  9. ncbi request reprint The crystal structure of the Calystegia sepium agglutinin reveals a novel quaternary arrangement of lectin subunits with a beta-prism fold
    Yves Bourne
    AFMB CNRS, CNRS UMR6098, F13402 Marseille Cedex 20, France
    J Biol Chem 279:527-33. 2004
  10. ncbi request reprint Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1)
    Y Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 13402, Marseille Cedex 20, France
    J Biol Chem 276:11949-55. 2001

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis
    Chris A Tarling
    Department of Chemistry, University of British Columbia, Vancouver V6T 1Z1, Canada
    J Biol Chem 278:47394-9. 2003
    ..This article presents the first direct identification of the catalytic nucleophile of an alpha-L-fucosidase, a key step in the understanding of these important enzymes...
  2. ncbi request reprint Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding
    Yves Bourne
    Ingénierie des Protéines, CNRS FRE 2738, Institut Federatif de Recherche Jean Roche, Université dela Méditerranée, Faculté d eMédecine Secteur Nord, F 13916 Marseille Cedex 20, France
    J Biol Chem 281:29256-67. 2006
    ..Hence, we provide a comprehensive set of structural snapshots of the steps leading to the intermediates of catalysis and the potential regulation by substrate binding to various allosteric sites at the enzyme surface...
  3. pmc Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger
    Isabelle Benoit
    UMR 1163 INRA Université de Provence de Biotechnologie des Champignons Filamenteux, IFR IBAIM, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France
    Appl Environ Microbiol 73:5624-32. 2007
    ....
  4. pmc Structural insights into the catalytic mechanism of bacterial guanosine-diphospho-D-mannose pyrophosphorylase and its regulation by divalent ions
    Marie Cécile Pelissier
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS, Universite Aix Marseille, F 13288 Marseille, France
    J Biol Chem 285:27468-76. 2010
    ..Involvement of both the N- and C-terminal domains, coupled to the catalytic role of a bivalent metal ion, highlights the catalytic features of bacterial GMPs compared with other members of the pyrophosphorylase superfamily...
  5. ncbi request reprint Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectins
    Y Bourne
    AFMB CNRS, Marseille, France
    Structure 7:1473-82. 1999
    ..Heltuba is therefore a good candidate as a defense protein against plant pathogens or predators...
  6. ncbi request reprint Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteins
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Structure 12:677-87. 2004
    ..Hence the structure of EstA constitutes a lead template in the design of new antifungal agents directed toward its pathogenic homologs...
  7. pmc Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, Marseille, France
    EMBO J 24:1512-22. 2005
    ....
  8. pmc Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, Marseille, France
    EMBO J 22:1-12. 2003
    ..Hence, these structures provide templates for designing compounds directed to the enzyme surface that modulate specific surface interactions controlling catalytic activity and non-catalytic heterologous protein associations...
  9. ncbi request reprint The crystal structure of the Calystegia sepium agglutinin reveals a novel quaternary arrangement of lectin subunits with a beta-prism fold
    Yves Bourne
    AFMB CNRS, CNRS UMR6098, F13402 Marseille Cedex 20, France
    J Biol Chem 279:527-33. 2004
    ..The present structure exemplifies the adaptability of the beta-prism building block in the evolution of plant lectins and highlights the biological role of these quaternary structures for carbohydrate recognition...
  10. ncbi request reprint Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1)
    Y Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 13402, Marseille Cedex 20, France
    J Biol Chem 276:11949-55. 2001
    ..This crevice, which is unique to frequenin and distinct from the myristoyl-binding box of recoverin, may accommodate a yet unknown protein ligand...
  11. ncbi request reprint Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions
    Y Bourne
    Centre National de la Recherche Scientifique, Marseille, France
    Structure 8:841-50. 2000
    ..The crystal structure of a cyclin-dependent kinase (Cdk) in complex with the human Cks homolog CksHs1 single-domain fold revealed the importance of conserved hydrophobic residues and charged residues within the beta-hinge motif...
  12. ncbi request reprint Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis
    Stephane Canaan
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    FEBS Lett 579:215-21. 2005
    ..Rv1155 thus appears to belong to a group of proteins with stringent conservation of the binding cleft, having evolved towards a new binding function...
  13. pmc High-throughput automated refolding screening of inclusion bodies
    Renaud Vincentelli
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS et Universités d Aix Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Protein Sci 13:2782-92. 2004
    ..Out of the 24 proteins, 17 remained soluble in at least one of the 96 refolding buffers, 15 passed large-scale purification tests, and five gave crystals...
  14. pmc Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions
    Philippe Leone
    Architecture et Fonction des Macromolecules Biologiques, CNRS Université d Aix Marseille, Campus Luminy, Marseille, France
    EMBO J 29:2461-71. 2010
    ..Although neuroligin-1 and neuroligin-2 have pre-determined conformations that respectively promote and prevent Nrx1beta association, unique conformational reshaping of the NL4 surface is required to permit Nrx1beta association...
  15. pmc Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, Centre National de la Recherche Scientifique, Universite d Aix Marseille, Campus Luminy Case 932, F 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 107:6076-81. 2010
    ....
  16. pmc Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose
    Yves Bourne
    AFMB, UMR CNRS 6098, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochem J 364:173-80. 2002
    ....
  17. pmc Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline state
    Yves Bourne
    Architecture et Fonction des Macromolécules Biologiques AFMB, CNRS UMR 6098, Universités d Aix Marseille, Campus Luminy Case 932, F 13288 Marseille Cedex 09, France
    J Am Chem Soc 132:18292-300. 2010
    ..Hence, for the tight-binding TZ2PA6 inhibitors, the initial complexes kinetically driven in solution slowly form more stable complexes governed by thermodynamic equilibrium and observable in mature crystals...
  18. ncbi request reprint A. niger protein "EstA", perhaps a new electrotactin, defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily
    Yves Bourne
    Ingénierie des Protéines, IFR Jean Roche, Universite de la Mediterranee, CNRS, Marseille, France
    Chem Biol Interact 157:395-6. 2005
    ....
  19. ncbi request reprint Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family
    Stephane Canaan
    Architecture et Fonction des Macromolecules Biologiques, AFMB UMR 6098, CNRS, 13402 Marseille, France
    Eur J Biochem 271:3953-61. 2004
    ..Overall, these data identify Rv1399c as a new nonlipolytic hydrolase from M. tuberculosis and we thus propose to reannotate its gene product as NLH-H...
  20. ncbi request reprint Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis
    Gerlind Sulzenbacher
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS, and Universités Aix Marseille I and II, 31 Chemin J Aiguier, F 13402 Marseille Cedex 20, France
    J Biol Chem 279:13119-28. 2004
    ....
  21. ncbi request reprint The Mtr2-Mex67 NTF2-like domain complex. Structural insights into a dual role of Mtr2 for yeast nuclear export
    Claire Senay
    AFMB CNRS, UMR 6098, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 278:48395-403. 2003
    ....
  22. ncbi request reprint Structural insights into conformational flexibility at the peripheral site and within the active center gorge of AChE
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS, Marseille, France
    Chem Biol Interact 157:159-65. 2005
    ....
  23. doi request reprint Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site
    Florence Vincent
    Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS et Universités Aix Marseille I et II, 163 Avenue de Luminy, 13288 Marseille, France
    Environ Microbiol 12:1775-86. 2010
    ..Modelling the DISMED2 of LadS reveals conservation of only one ligand binding site, suggesting a distinct mechanism underlying the activity of this sensor kinase...
  24. pmc LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis
    Gerlind Sulzenbacher
    AFMB, CNRS UMR 6098, Marseille Cedex, France
    EMBO J 25:1436-44. 2006
    ....
  25. ncbi request reprint Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein
    Yves Bourne
    Architecture et Fonction des Macromolecules Biologiques, CNRS, Marseille, France
    J Mol Neurosci 30:103-4. 2006
    ..Although they belong to various structural and/or functional subfamilies, they often share similar molecular features, such as a highly reticulated scaffold presenting specific binding determinants...
  26. ncbi request reprint Structural insights into AChE inhibition by monoclonal antibodies
    Ludovic Renault
    Ingénierie des Protéines, CNRS, IFR Jean Roche, Universite de la Mediterranee, Marseille, France
    Chem Biol Interact 157:397-400. 2005
    ..Preliminary data are presented...
  27. doi request reprint Structure-function relationships of the alpha/beta-hydrolase fold domain of neuroligin: a comparison with acetylcholinesterase
    Philippe Leone
    Architecture et Fonction des Macromolécules Biologiques AFMB, CNRS Universités Aix Marseille, Campus Luminy, Marseille, France
    Chem Biol Interact 187:49-55. 2010
    ....
  28. doi request reprint Glycosyltransferases, glycoside hydrolases: surprise, surprise!
    Bernard Henrissat
    Architecture et Fonction des Macromolecules Biologiques, CNRS, Université d Aix Marseille I and II, 163 Avenue de Luminy, 13288 Marseille, France
    Curr Opin Struct Biol 18:527-33. 2008
    ..It is wrong! The past year has come as a refreshing wake-up call with major surprises for both GHs and GTs...
  29. doi request reprint Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown
    Florence Vincent
    Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS and Aix Marseille Universities, Marseille, France
    FEBS Lett 584:1577-84. 2010
    ..This unprecedented association coupling oxidoreduction and carbohydrate recognition events may have implications for effective nutrient uptake in the marine environment...
  30. ncbi request reprint Functional characterization of the glycosyltransferase domain of penicillin-binding protein 1a from Thermotoga maritima
    Julien Offant
    Architecture et Fonction des Macromolecules Biologiques, Case 932, Campus de Luminy, F13288 Marseille Cedex 09, France
    Biochim Biophys Acta 1764:1036-42. 2006
    ..Overall, our preliminary data document the biochemical properties of GT-PBP1a* and should guide further studies aimed at deciphering the structural determinants involved into membrane binding by this class of enzymes...
  31. ncbi request reprint Crystal structure of E. coli yddE protein reveals a striking homology with diaminopimelate epimerase
    Alice Grassick
    Architecture et Fonction des Macromolecules Biologiques, CNRS UMR6098, Marseille, France
    Proteins 55:764-7. 2004
  32. doi request reprint The structure of human DNase I bound to magnesium and phosphate ions points to a catalytic mechanism common to members of the DNase I-like superfamily
    Goetz Parsiegla
    Architecture et Fonction des Macromolecules Biologiques, Aix Marseille Université and CNRS UMR 7257, Parc Scientifique et Technonlogique de Luminy, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France
    Biochemistry 51:10250-8. 2012
    ..Overall, our combined structural and mutagenesis data suggest the occurrence of a magnesium-assisted pentavalent phosphate transition state in human DNase I during catalysis, where Asp168 may play a key role as a general catalytic base...
  33. ncbi request reprint [Bacterial glycosidases for the production of universal blood]
    Gerlind Sulzenbacher
    Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS, Universités Aix Marseille I et II, Case 932, 163, Avenue de Luminy, 13288, Marseille Cedex 9, France
    Med Sci (Paris) 23:703-5. 2007
  34. ncbi request reprint A medium-throughput crystallization approach
    Gerlind Sulzenbacher
    AFMB, UMR 6098, CNRS and Universités Aix Marseille I and II, 31 Chemin J Aiguier, F 13402 Marseille Cedex 20, France
    Acta Crystallogr D Biol Crystallogr 58:2109-15. 2002
    ..These still partial results might indicate present and future directions for those who have to make crucial choices concerning their crystallization platform in structural genomics programs...
  35. doi request reprint Proteins with an alpha/beta hydrolase fold: Relationships between subfamilies in an ever-growing superfamily
    Nicolas Lenfant
    Dynamique Musculaire et Métabolisme, INRA, Place Viala, Montpellier, France
    Chem Biol Interact 203:266-8. 2013
    ..Another of our goals is to implement new tools to distinguish catalytically active enzymes from non-catalytic proteins in poorly studied or annotated subfamilies...
  36. pmc ESTHER, the database of the α/β-hydrolase fold superfamily of proteins: tools to explore diversity of functions
    Nicolas Lenfant
    Dynamique Musculaire et Métabolisme, INRA UM1, Place Viala, 34060 Montpellier, France
    Nucleic Acids Res 41:D423-9. 2013
    ....
  37. ncbi request reprint Mechanism of acetylcholinesterase inhibition by fasciculin: a 5-ns molecular dynamics simulation
    Kaihsu Tai
    Howard Hughes Medical Institute and Department of Chemistry, University of California, San Diego, La Jolla, California 92093 0365, USA
    J Am Chem Soc 124:6153-61. 2002
    ..Additional data from these simulations can be found at http://mccammon.ucsd.edu/...
  38. pmc Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation
    Yves Bourne
    Ingénierie des Protéines, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6560, Institut Federatif de Recherche Jean Roche, Universite de la Mediterranee, Faculte de Medecine Secteur Nord, F 13916 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 101:1449-54. 2004
    ....
  39. pmc The size, shape and specificity of the sugar-binding site of the jacalin-related lectins is profoundly affected by the proteolytic cleavage of the subunits
    Corinne Houlès Astoul
    Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 205 route de Narbonne, 31077 Toulouse Cedex 4, France
    Biochem J 367:817-24. 2002
    ..Owing to this broad carbohydrate-binding specificity, jacalin is capable of recognizing complex glycans from plant pathogens or predators...
  40. ncbi request reprint Bacterial glycosidases for the production of universal red blood cells
    Qiyong P Liu
    ZymeQuest Inc, 100 Cummings Center, Suite 436H, Beverly, Massachusetts 01915, USA
    Nat Biotechnol 25:454-64. 2007
    ..The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions...
  41. pmc Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations
    Scott B Hansen
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093 0636, USA
    EMBO J 24:3635-46. 2005
    ..This comprehensive set of structures reflects a dynamic template for delineating further conformational changes of the LBD of the nicotinic receptor...
  42. ncbi request reprint Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californica
    Scott B Hansen
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093 0636, USA
    J Mol Neurosci 30:101-2. 2006
    ..stagnalis, including ligand specificity for the nAChR alpha7 subtype-specific alpha-conotoxin ImI (Hansen et al., 2004)...
  43. pmc Structural analysis of the synaptic protein neuroligin and its beta-neurexin complex: determinants for folding and cell adhesion
    Igor P Fabrichny
    Biochimie des Interactions Moléculaires et Cellulaires, CNRS FRE 2738, Institut Federatif de Recherche Jean Roche, Universite de la Mediterranee, Faculte de Medecine Secteur Nord, F 13916 Marseille Cedex 20, France
    Neuron 56:979-91. 2007
    ..These structures exemplify how an alpha/beta-hydrolase fold varies in surface topography to confer adhesion properties and provide templates for analyzing abnormal processing or recognition events associated with autism...