Research Topics
Genomes and Genes | Yves BourneSummaryAffiliation: Centre National de la Recherche Scientifique Country: France Publications
| Collaborators
|
Detail Information
Publications
Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesisChris A Tarling
Department of Chemistry, University of British Columbia, Vancouver V6T 1Z1, Canada
J Biol Chem 278:47394-9. 2003..This article presents the first direct identification of the catalytic nucleophile of an alpha-L-fucosidase, a key step in the understanding of these important enzymes...- Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium bindingYves Bourne
Ingénierie des Protéines, CNRS FRE 2738, Institut Federatif de Recherche Jean Roche, Université dela Méditerranée, Faculté d eMédecine Secteur Nord, F 13916 Marseille Cedex 20, France
J Biol Chem 281:29256-67. 2006..Hence, we provide a comprehensive set of structural snapshots of the steps leading to the intermediates of catalysis and the potential regulation by substrate binding to various allosteric sites at the enzyme surface... - Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus nigerIsabelle Benoit
UMR 1163 INRA Université de Provence de Biotechnologie des Champignons Filamenteux, IFR IBAIM, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France
Appl Environ Microbiol 73:5624-32. 2007.... - Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectinsY Bourne
AFMB CNRS, Marseille, France
Structure 7:1473-82. 1999..Heltuba is therefore a good candidate as a defense protein against plant pathogens or predators... - The crystal structure of the Calystegia sepium agglutinin reveals a novel quaternary arrangement of lectin subunits with a beta-prism foldYves Bourne
AFMB CNRS, CNRS UMR6098, F13402 Marseille Cedex 20, France
J Biol Chem 279:527-33. 2004..The present structure exemplifies the adaptability of the beta-prism building block in the evolution of plant lectins and highlights the biological role of these quaternary structures for carbohydrate recognition... - Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptorsYves Bourne
Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, Marseille, France
EMBO J 24:1512-22. 2005.... - Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic siteYves Bourne
Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, Marseille, France
EMBO J 22:1-12. 2003..Hence, these structures provide templates for designing compounds directed to the enzyme surface that modulate specific surface interactions controlling catalytic activity and non-catalytic heterologous protein associations... - Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteinsYves Bourne
Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
Structure 12:677-87. 2004..Hence the structure of EstA constitutes a lead template in the design of new antifungal agents directed toward its pathogenic homologs... - Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactionsY Bourne
Centre National de la Recherche Scientifique, Marseille, France
Structure 8:841-50. 2000..The crystal structure of a cyclin-dependent kinase (Cdk) in complex with the human Cks homolog CksHs1 single-domain fold revealed the importance of conserved hydrophobic residues and charged residues within the beta-hinge motif... - Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1)Y Bourne
Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 13402, Marseille Cedex 20, France
J Biol Chem 276:11949-55. 2001..This crevice, which is unique to frequenin and distinct from the myristoyl-binding box of recoverin, may accommodate a yet unknown protein ligand... - Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosisStephane Canaan
Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
FEBS Lett 579:215-21. 2005..Rv1155 thus appears to belong to a group of proteins with stringent conservation of the binding cleft, having evolved towards a new binding function... - Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactionsPhilippe Leone
Architecture et Fonction des Macromolecules Biologiques, CNRS Université d Aix Marseille, Campus Luminy, Marseille, France
EMBO J 29:2461-71. 2010..Although neuroligin-1 and neuroligin-2 have pre-determined conformations that respectively promote and prevent Nrx1beta association, unique conformational reshaping of the NL4 surface is required to permit Nrx1beta association... - High-throughput automated refolding screening of inclusion bodiesRenaud Vincentelli
, UMR 6098, , 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Protein Sci 13:2782-92. 2004..Out of the 24 proteins, 17 remained soluble in at least one of the 96 refolding buffers, 15 passed large-scale purification tests, and five gave crystals... - Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonismYves Bourne
Architecture et Fonction des Macromolecules Biologiques, Centre National de la Recherche Scientifique, Universite d Aix Marseille, Campus Luminy Case 932, F 13288 Marseille Cedex 9, France
Proc Natl Acad Sci U S A 107:6076-81. 2010.... - Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline stateYves Bourne
Architecture et Fonction des Macromolécules Biologiques AFMB, CNRS UMR 6098, Universités d Aix Marseille, Campus Luminy Case 932, F 13288 Marseille Cedex 09, France
J Am Chem Soc 132:18292-300. 2010..Hence, for the tight-binding TZ2PA6 inhibitors, the initial complexes kinetically driven in solution slowly form more stable complexes governed by thermodynamic equilibrium and observable in mature crystals... - A. niger protein "EstA", perhaps a new electrotactin, defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamilyYves Bourne
, IFR Jean Roche, , CNRS, Marseille, France
Chem Biol Interact 157:395-6. 2005.... - Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL familyStephane Canaan
Architecture et Fonction des Macromolecules Biologiques, AFMB UMR 6098, CNRS, 13402 Marseille, France
Eur J Biochem 271:3953-61. 2004..Overall, these data identify Rv1399c as a new nonlipolytic hydrolase from M. tuberculosis and we thus propose to reannotate its gene product as NLH-H... - Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosisGerlind Sulzenbacher
Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS, and Universités Aix Marseille I and II, 31 Chemin J Aiguier, F 13402 Marseille Cedex 20, France
J Biol Chem 279:13119-28. 2004.... - The Mtr2-Mex67 NTF2-like domain complex. Structural insights into a dual role of Mtr2 for yeast nuclear exportClaire Senay
AFMB CNRS, UMR 6098, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Biol Chem 278:48395-403. 2003.... - Structural insights into conformational flexibility at the peripheral site and within the active center gorge of AChEYves Bourne
Architecture et Fonction des Macromolecules Biologiques, CNRS, Marseille, France
Chem Biol Interact 157:159-65. 2005.... - Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding siteFlorence Vincent
Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS et Universités Aix Marseille I et II, 163 Avenue de Luminy, 13288 Marseille, France
Environ Microbiol 12:1775-86. 2010..Modelling the DISMED2 of LadS reveals conservation of only one ligand binding site, suggesting a distinct mechanism underlying the activity of this sensor kinase... - Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannoseYves Bourne
AFMB, UMR-CNRS 6098, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Biochem J 364:173-80. 2002.... - LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosisGerlind Sulzenbacher
AFMB, CNRS UMR 6098, Marseille Cedex, France
EMBO J 25:1436-44. 2006.... - Structure-function relationships of the alpha/beta-hydrolase fold domain of neuroligin: a comparison with acetylcholinesterasePhilippe Leone
Architecture et Fonction des Macromolécules Biologiques AFMB, CNRS Universités Aix Marseille, Campus Luminy, Marseille, France
Chem Biol Interact 187:49-55. 2010.... - Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition proteinYves Bourne
, CNRS, Marseille, France
J Mol Neurosci 30:103-4. 2006..Although they belong to various structural and/or functional subfamilies, they often share similar molecular features, such as a highly reticulated scaffold presenting specific binding determinants... - Structural insights into AChE inhibition by monoclonal antibodiesLudovic Renault
, CNRS, IFR Jean Roche, , Marseille, France
Chem Biol Interact 157:397-400. 2005..Preliminary data are presented... - Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdownFlorence Vincent
Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS and Aix Marseille Universities, Marseille, France
FEBS Lett 584:1577-84. 2010..This unprecedented association coupling oxidoreduction and carbohydrate recognition events may have implications for effective nutrient uptake in the marine environment... - Structural insights into the catalytic mechanism of bacterial guanosine-diphospho-D-mannose pyrophosphorylase and its regulation by divalent ionsMarie Cécile Pelissier
Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS, Universite Aix Marseille, F 13288 Marseille, France
J Biol Chem 285:27468-76. 2010..Involvement of both the N- and C-terminal domains, coupled to the catalytic role of a bivalent metal ion, highlights the catalytic features of bacterial GMPs compared with other members of the pyrophosphorylase superfamily... - Crystal structure of E. coli yddE protein reveals a striking homology with diaminopimelate epimeraseAlice Grassick
, CNRS UMR6098, Marseille, France
Proteins 55:764-7. 2004 - Glycosyltransferases, glycoside hydrolases: surprise, surprise!Bernard Henrissat
Architecture et Fonction des Macromolecules Biologiques, CNRS, Université d Aix Marseille I and II, 163 Avenue de Luminy, 13288 Marseille, France
Curr Opin Struct Biol 18:527-33. 2008..It is wrong! The past year has come as a refreshing wake-up call with major surprises for both GHs and GTs... - Functional characterization of the glycosyltransferase domain of penicillin-binding protein 1a from Thermotoga maritimaJulien Offant
Architecture et Fonction des Macromolecules Biologiques, Case 932, Campus de Luminy, F13288 Marseille Cedex 09, France
Biochim Biophys Acta 1764:1036-42. 2006..Overall, our preliminary data document the biochemical properties of GT-PBP1a* and should guide further studies aimed at deciphering the structural determinants involved into membrane binding by this class of enzymes... - A medium-throughput crystallization approachGerlind Sulzenbacher
AFMB, UMR 6098, CNRS and Universités Aix Marseille I and II, 31 Chemin J Aiguier, F 13402 Marseille Cedex 20, France
Acta Crystallogr D Biol Crystallogr 58:2109-15. 2002..These still partial results might indicate present and future directions for those who have to make crucial choices concerning their crystallization platform in structural genomics programs... - [Bacterial glycosidases for the production of universal blood]Gerlind Sulzenbacher
, UMR6098, CNRS, , Case 932, 163, Avenue de Luminy, 13288, Marseille Cedex 9, France
Med Sci (Paris) 23:703-5. 2007 - Freeze-frame inhibitor captures acetylcholinesterase in a unique conformationYves Bourne
, , , , , F-13916 Marseille Cedex 20, France
Proc Natl Acad Sci U S A 101:1449-54. 2004.... - Mechanism of acetylcholinesterase inhibition by fasciculin: a 5-ns molecular dynamics simulationKaihsu Tai
Howard Hughes Medical Institute and Department of Chemistry, University of California, San Diego, La Jolla, California 92093-0365, USA
J Am Chem Soc 124:6153-61. 2002..Additional data from these simulations can be found at http://mccammon.ucsd.edu/... - Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformationsScott B Hansen
Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093-0636, USA
EMBO J 24:3635-46. 2005..This comprehensive set of structures reflects a dynamic template for delineating further conformational changes of the LBD of the nicotinic receptor... - Structural analysis of the synaptic protein neuroligin and its beta-neurexin complex: determinants for folding and cell adhesionIgor P Fabrichny
Biochimie des Interactions Moléculaires et Cellulaires, CNRS FRE 2738, Institut Federatif de Recherche Jean Roche, Universite de la Mediterranee, Faculte de Medecine Secteur Nord, F 13916 Marseille Cedex 20, France
Neuron 56:979-91. 2007..These structures exemplify how an alpha/beta-hydrolase fold varies in surface topography to confer adhesion properties and provide templates for analyzing abnormal processing or recognition events associated with autism... - Bacterial glycosidases for the production of universal red blood cellsQiyong P Liu
ZymeQuest Inc, 100 Cummings Center, Suite 436H, Beverly, Massachusetts 01915, USA
Nat Biotechnol 25:454-64. 2007..The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions... - Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californicaScott B Hansen
Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093 0636, USA
J Mol Neurosci 30:101-2. 2006..stagnalis, including ligand specificity for the nAChR alpha7 subtype-specific alpha-conotoxin ImI (Hansen et al., 2004)...