Alain Beck

Summary

Affiliation: Centre de Recherche Pierre Fabre
Country: France

Publications

  1. ncbi request reprint The next generation of antibody-drug conjugates comes of age
    Alain Beck
    Department of Physico Chemistry, Centre d Immunologie Pierre Fabre CIPF, St Julien en Genevois 74164, France
    Discov Med 10:329-39. 2010
  2. pmc Fourth World Antibody-Drug Conjugate Summit: February 29-March 1, 2012, Frankfurt, Germany
    Alain Beck
    Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    MAbs 4:637-47. 2012
  3. doi request reprint Biosimilar, biobetter, and next generation antibody characterization by mass spectrometry
    Alain Beck
    Centre d Immunologie Pierre Fabre CIPF, 5 Av Napoleon III, BP 60497, 74164 Saint Julien en Genevois, France
    Anal Chem 84:4637-46. 2012
  4. ncbi request reprint GlycoFi's technology to control the glycosylation of recombinant therapeutic proteins
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, F74160, Saint Julien en Genevois, France 33 4 50 35 35 55 33 4 50 35 35 90 www cipf com
    Expert Opin Drug Discov 5:95-111. 2010
  5. ncbi request reprint Stability and CTL-activity of P40/ELA melanoma vaccine candidate
    A Beck
    Centre d Immunologie Pierre Fabre CIPF, bioMérieux Pierre Fabre, 5 Avenue Napoleon III, F 74164 Saint Julien en Genevois, France
    Biologicals 29:293-8. 2001
  6. ncbi request reprint Antibody Engineering & Design 2010. 24-25 February 2010, Frankfurt, Germany
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74160 Saint Julien en Genevois, France
    IDrugs 13:295-9. 2010
  7. doi request reprint Strategies and challenges for the next generation of therapeutic antibodies
    Alain Beck
    Centre d Immunologie Pierre Fabre CIPF, 5 Avenue Napoleon III, F74160, Saint Julien en Genevois, France
    Nat Rev Immunol 10:345-52. 2010
  8. ncbi request reprint Peptides as tools and drugs for immunotherapies
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, F74164 Saint Julien en Genevois, Cedex, France
    J Pept Sci 13:588-602. 2007
  9. doi request reprint [Therapeutic antibodies and related products: choosing the right structure for success]
    Alain Beck
    Centre d immunologie Pierre Fabre www cipf com, 5, Avenue Napoleon III, 74160 Saint Julien en Genevois, France
    Med Sci (Paris) 25:1024-32. 2009
  10. ncbi request reprint Trends in glycosylation, glycoanalysis and glycoengineering of therapeutic antibodies and Fc-fusion proteins
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, F74160, Saint Julien en Genevois, France
    Curr Pharm Biotechnol 9:482-501. 2008

Collaborators

Detail Information

Publications50

  1. ncbi request reprint The next generation of antibody-drug conjugates comes of age
    Alain Beck
    Department of Physico Chemistry, Centre d Immunologie Pierre Fabre CIPF, St Julien en Genevois 74164, France
    Discov Med 10:329-39. 2010
    ..Several examples of ADCs are presented with emphasis on three major molecules currently in late clinical development as well as next generation thio-mAbs conjugates with improved therapeutic index...
  2. pmc Fourth World Antibody-Drug Conjugate Summit: February 29-March 1, 2012, Frankfurt, Germany
    Alain Beck
    Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    MAbs 4:637-47. 2012
    ..Last but not least, progresses in solving Chemistry Manufacturing and Control, and pharmacokinetic issues were addressed by scientists from Genentech, Pfizer, Novartis and Pierre Fabre...
  3. doi request reprint Biosimilar, biobetter, and next generation antibody characterization by mass spectrometry
    Alain Beck
    Centre d Immunologie Pierre Fabre CIPF, 5 Av Napoleon III, BP 60497, 74164 Saint Julien en Genevois, France
    Anal Chem 84:4637-46. 2012
    ..In addition, the structural and functional insights gained both by state-of-the art and emerging MS methods used for biobetters and next generation antibodies design and optimization will also be highlighted...
  4. ncbi request reprint GlycoFi's technology to control the glycosylation of recombinant therapeutic proteins
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, F74160, Saint Julien en Genevois, France 33 4 50 35 35 55 33 4 50 35 35 90 www cipf com
    Expert Opin Drug Discov 5:95-111. 2010
    ..These properties associated with the perspective to industrialize the manufacturing process of Pichia makes it a very promising expression system to produce large-scale batches of therapeutics at a lower cost...
  5. ncbi request reprint Stability and CTL-activity of P40/ELA melanoma vaccine candidate
    A Beck
    Centre d Immunologie Pierre Fabre CIPF, bioMérieux Pierre Fabre, 5 Avenue Napoleon III, F 74164 Saint Julien en Genevois, France
    Biologicals 29:293-8. 2001
    ..We report here that the hydrochloride shows a higher stability than the acetate and may be suitable for use in man...
  6. ncbi request reprint Antibody Engineering & Design 2010. 24-25 February 2010, Frankfurt, Germany
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74160 Saint Julien en Genevois, France
    IDrugs 13:295-9. 2010
    ..Investigational drugs discussed include 1D4.1-ABT325 (Abbott Laboratories), Sym-004 (Symphogen A/S) and CNTO-607 (Johnson & Johnson)...
  7. doi request reprint Strategies and challenges for the next generation of therapeutic antibodies
    Alain Beck
    Centre d Immunologie Pierre Fabre CIPF, 5 Avenue Napoleon III, F74160, Saint Julien en Genevois, France
    Nat Rev Immunol 10:345-52. 2010
    ..Here, we discuss strategies to select the best therapeutic antigen targets, to optimize the structure of IgG antibodies and to design related or new structures with additional functions...
  8. ncbi request reprint Peptides as tools and drugs for immunotherapies
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, F74164 Saint Julien en Genevois, Cedex, France
    J Pept Sci 13:588-602. 2007
    ....
  9. doi request reprint [Therapeutic antibodies and related products: choosing the right structure for success]
    Alain Beck
    Centre d immunologie Pierre Fabre www cipf com, 5, Avenue Napoleon III, 74160 Saint Julien en Genevois, France
    Med Sci (Paris) 25:1024-32. 2009
    ..Based on the current knowledge, choosing the right antibody format, isotype and glycosylation profile are some of the key issues to address early during the lead selection...
  10. ncbi request reprint Trends in glycosylation, glycoanalysis and glycoengineering of therapeutic antibodies and Fc-fusion proteins
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, F74160, Saint Julien en Genevois, France
    Curr Pharm Biotechnol 9:482-501. 2008
    ....
  11. ncbi request reprint Characterization by liquid chromatography combined with mass spectrometry of monoclonal anti-IGF-1 receptor antibodies produced in CHO and NS0 cells
    Alain Beck
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74160 Saint Julien en Genevois, France
    J Chromatogr B Analyt Technol Biomed Life Sci 819:203-18. 2005
    ....
  12. pmc 6th Annual European Antibody Congress 2010: November 29-December 1, 2010, Geneva, Switzerland
    Alain Beck
    Physio Chemistry Department, Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    MAbs 3:111-32. 2011
    ....
  13. pmc World Antibody Drug Conjugate Summit Europe: February 21-23, 2011; Frankfurt, Germany
    Alain Beck
    Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    MAbs 3:331-7. 2011
    ..In addition, presentations on duocarmycin based-ADCs, alpha emitting immunoconjugates and antibody-conjugated nanoparticles were given by representatives from Syntarga, Algeta and the University of Stuttgart, respectively...
  14. ncbi request reprint Synthesis and characterization of Respiratory Syncytial Virus protein G related peptides containing two disulfide bridges
    A Beck
    Department of Physical Chemistry, Center d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    J Pept Res 55:24-35. 2000
    ....
  15. ncbi request reprint Stability and CTL activity of N-terminal glutamic acid containing peptides
    A Beck
    Centre d Immunologie Pierre Fabre CIPF, Saint Julien en Genevois, France
    J Pept Res 57:528-38. 2001
    ....
  16. pmc Gamma interferon-dependent protection of the mouse upper respiratory tract following parenteral immunization with a respiratory syncytial virus G protein fragment
    Helene Plotnicky-Gilquin
    Centre d Immunologie Pierre Fabre, F74164 Saint Julien en Genevois, France
    J Virol 76:10203-10. 2002
    ..Together, these results demonstrated for the first time that the T-helper-cell epitope of RSV G protein induces URT protection in mice after parenteral immunization through a Th1-type, IFN-gamma-dependent mechanism...
  17. pmc Identification of B- and T-cell epitopes of BB, a carrier protein derived from the G protein of Streptococcus strain G148
    Liliane Goetsch
    Centre d Immunologie Pierre Fabre, 74164 Saint Julien en Genevois, France
    Clin Diagn Lab Immunol 10:125-32. 2003
    ..One of these T-cell epitopes is common to BALB/c mice and humans and was localized in the region that contains the albumin-binding site. These data are of interest for the optimization of new carrier molecules derived from BB...
  18. ncbi request reprint Passive transfer of serum antibodies induced by BBG2Na, a subunit vaccine, in the elderly protects SCID mouse lungs against respiratory syncytial virus challenge
    Helene Plotnicky-Gilquin
    Centre d Immunologie Pierre Fabre, 5 Av Napoleon III, F74 164, Saint Julien en Genevois, France
    Virology 303:130-7. 2002
    ..Thus, BBG2Na is immunogenic in the elderly and confers passive protection in mice after serum transfer. To our knowledge, this is the first description of protective Abs induced by a subunit vaccine in human...
  19. doi request reprint [Immunoconjugates, drug-armed antibodies to fight against cancer]
    Jean Francois Haeuw
    Centre d Immunologie Pierre Fabre, 5, Avenue Napoleon III, BP 60497, 74160 Saint Julien en Genevois, France jean francois haeuw pierre fabre com
    Med Sci (Paris) 25:1046-52. 2009
    ..Today, we know that the efficacy of an immunoconjugate depends not only on the coupled cytotoxic agent, but also on the selected target, the coupling method and the linker...
  20. pmc CD4(+) T-cell-mediated antiviral protection of the upper respiratory tract in BALB/c mice following parenteral immunization with a recombinant respiratory syncytial virus G protein fragment
    H Plotnicky-Gilquin
    Centre d Immunologie Pierre Fabre, 74 164 St Julien en Genevois, France
    J Virol 74:3455-63. 2000
    ..Taken together, these results demonstrate for the first time that CD4(+) T cells induced upon parenteral immunization with an RSV G protein fragment play a critical role in URT protection of normal mice against RSV infection...
  21. doi request reprint Cetuximab Fab and Fc N-glycan fast characterization using IdeS digestion and liquid chromatography coupled to electrospray ionization mass spectrometry
    Marie Claire Janin-Bussat
    Physico Chemistry Department, Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    Methods Mol Biol 988:93-113. 2013
    ..The method can also be applied for biosimilars, biobetters, and next-generation antibodies and Fc-fusion proteins...
  22. doi request reprint The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies
    Elsa Wagner-Rousset
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74160 Saint Julien en Genevois, France
    J Chromatogr B Analyt Technol Biomed Life Sci 872:23-37. 2008
    ....
  23. ncbi request reprint DDA adjuvant induces a mixed Th1/Th2 immune response when associated with BBG2Na, a respiratory syncytial virus potential vaccine
    Christine Klinguer-Hamour
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, BP 497, F 74164 Saint Julien en Genevois, France
    Vaccine 20:2743-51. 2002
    ..Furthermore, BBG2Na showed the same protective efficacy against RSV challenge when formulated either in DDA or in alum in mice and cotton rats...
  24. doi request reprint NanoLC Chips MS/MS for the characterization of N-glycopeptides generated from trypsin digestion of a monoclonal antibody
    Elsa Wagner-Rousset
    Physico Chemistry Department, Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    Methods Mol Biol 988:81-91. 2013
    ....
  25. ncbi request reprint Synthesis, refolding and protective immune responses of a potential antigen for human Respiratory Syncytial Virus vaccine
    C Klinguer-Hamour
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, BP 497, F 74164 Saint Julien en Genevois, France
    J Pept Res 62:27-36. 2003
    ..Interestingly, we showed in the present study that G20 generated a highly protective antibody response against RSV challenge in Balb/c mice. Therefore, G20 represents a new potential antigen for an RSV vaccine...
  26. ncbi request reprint Expression of recombinant proteins in a lipid A mutant of Escherichia coli BL21 with a strongly reduced capacity to induce dendritic cell activation and maturation
    Isabelle Cognet
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, Saint Julien en, Genevois, 74164, France
    J Immunol Methods 272:199-210. 2003
    ..It is likely to be useful for the identification of new proteins activating the innate immune response and to reducing the risk linked with low level of endotoxin contamination in therapeutic recombinant proteins...
  27. ncbi request reprint Lipophilic quaternary ammonium salt acts as a mucosal adjuvant when co-administered by the nasal route with vaccine antigens
    C Klinguer
    , Centre d'Immunologie Pierre Fabre, , B.P. 497, St. Julien en Genevois, France
    Vaccine 19:4236-44. 2001
    ..Furthermore, we provide evidence that the DDA+BBG2Na vaccine does not induce lung immunopathology upon subsequent RSV challenge...
  28. ncbi request reprint A recombinant humanized anti-insulin-like growth factor receptor type I antibody (h7C10) enhances the antitumor activity of vinorelbine and anti-epidermal growth factor receptor therapy against human cancer xenografts
    Liliane Goetsch
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74160, St Julien en Genevois, France
    Int J Cancer 113:316-28. 2005
    ....
  29. ncbi request reprint Insulin-like growth factor receptor type I as a target for cancer therapy
    Nathalie Corvaia
    Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    Front Biosci (Schol Ed) 5:439-50. 2013
    ..This review will provide an overview of the current knowledge on IGF-IR axis for development of novel therapeutics in Oncology...
  30. doi request reprint [Monoclonal antibodies for treating infectious diseases]
    Christine Klinguer-Hamour
    Centre d Immunologie Pierre Fabre, 5, Avenue Napoleon III, BP 60497, 74160 Saint Julien en Genevois, France
    Med Sci (Paris) 25:1116-20. 2009
    ..The synergies obtained using combinations of anti-infectious mAb and small molecule drugs will certainly offer new opportunities for the treatment of infectious diseases...
  31. ncbi request reprint Development of novel protein scaffolds as alternatives to whole antibodies for imaging and therapy: status on discovery research and clinical validation
    T Wurch
    Centre d Immunologie Pierre Fabre, F74164 Saint Julien en Genevois, France
    Curr Pharm Biotechnol 9:502-9. 2008
    ....
  32. ncbi request reprint Safety and immunogenicity of a novel recombinant subunit respiratory syncytial virus vaccine (BBG2Na) in healthy young adults
    U F Power
    Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    J Infect Dis 184:1456-60. 2001
    ..Antibody responses to 2 murine lung protective epitopes were also highly boosted after vaccination. Therefore, BBG2Na is safe, well tolerated, and highly immunogenic in RSV-seropositive adults...
  33. ncbi request reprint The recombinant Klebsiella pneumoniae outer membrane protein OmpA has carrier properties for conjugated antigenic peptides
    J F Haeuw
    Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    Eur J Biochem 255:446-54. 1998
    ....
  34. ncbi request reprint Identification and characterisation of multiple linear B cell protectopes in the respiratory syncytial virus G protein
    U F Power
    Centre d Immunologie Pierre Fabre, 74164 Cedex, Saint Julien en Genevois, France
    Vaccine 19:2345-51. 2001
    ..However, all the identified murine B cell protectopes were conserved in RSV seropositive humans. Should these protectopes correspond with protection in humans, BBG2Na may constitute a very interesting vaccine candidate against RSV...
  35. pmc Targeting of nasal mucosa-associated antigen-presenting cells in vivo with an outer membrane protein A derived from Klebsiella pneumoniae
    L Goetsch
    Centre d Immunologie Pierre Fabre, 74164 Saint Julien en Genevois, France
    Infect Immun 69:6434-44. 2001
    ..n. immunization with a subunit of the RSV-A molecule in the absence of any mucosal adjuvant results in both upper and lower respiratory tract protection against RSV-A infection...
  36. ncbi request reprint Development of a quantitative assay for residual host cell proteins in a recombinant subunit vaccine against human respiratory syncytial virus
    N Dagouassat
    Centre d Immunologie Pierre Fabre CIPF, 5 Avenue Napoleon III, B P 497, F 74164, Saint Julien en Genevois, France
    J Immunol Methods 251:151-9. 2001
    ..The quantification limit was 1.3 ng/ml of HCP, giving a sensitivity range of the assay of 10 to 30 ppm. To our knowledge, this is the first sensitive HCP assay reported for a vaccine...
  37. ncbi request reprint The immunodominant influenza matrix T cell epitope recognized in human induces influenza protection in HLA-A2/K(b) transgenic mice
    H Plotnicky
    Centre d Immunologie Pierre Fabre, 5, Av Napoléon III, 74 164, St Julien en Genevois, France
    Virology 309:320-9. 2003
    ..They encourage further in vivo evaluation of T cell epitopes recognized in the context of human MHC molecules...
  38. doi request reprint Capture of the human IgG1 antibodies by protein A for the kinetic study of h-IgG/FcγR interaction using SPR-based biosensor technology
    Thierry Champion
    Physico Chemistry Department, Centre d Immunologie Pierre Fabre, Saint Julien en Genevois, France
    Methods Mol Biol 988:331-43. 2013
    ..This setup allows the definition of the kinetic rates of binding of the FcγRI (high affinity) or the FcγRIIIa (low affinity) on the Fc domain of IgGs...
  39. ncbi request reprint Residual DNA quantification in clinical batches of BBG2Na, a recombinant subunit vaccine against human respiratory syncytial virus
    M Lokteff
    Centre d'Immunologie Pierre Fabre, , Saint-Julien-en-Genevois, F-74164, France
    Biologicals 29:123-32. 2001
    ..This study also provides data concerning the validation of the hybridisation dot blot assay and the total DNA Threshold(trade mark)assay...
  40. ncbi request reprint Streptococcus pneumoniae polysaccharides conjugated to the outer membrane protein A from Klebsiella pneumoniae elicit protective antibodies
    Christine Libon
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, St Julien en Genevois, France
    Vaccine 20:2174-80. 2002
    ..In addition, the humoral response generated is able to protect mice from a bacterial challenge. Our results indicate that P40 could be included in the development of new PS conjugate vaccines...
  41. ncbi request reprint Measurement of nuclear factor-kappa B translocation on lipopolysaccharide-activated human dendritic cells by confocal microscopy and flow cytometry
    Aline Blaecke
    bioMérieux Pierre Fabre, Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74160 St Julien en Genevois, France
    Cytometry 48:71-9. 2002
    ..The aim of this article is to define optimal conditions for the measurement of NF-kappaB translocation by both confocal microscopy and flow cytometry...
  42. ncbi request reprint Structure-antigenicity relationship studies of the central conserved region of human respiratory syncytial virus protein G
    M Sugawara
    Institut de Pharmacologie et de Biologie Structurale Centre National de la Recherche Scientifique, Toulouse, France
    J Pept Res 60:271-82. 2002
    ....
  43. ncbi request reprint Ribosomal proteins and DNA-binding protein II from the extreme thermophile Bacillus caldolyticus
    A Beck
    Biol Chem Hoppe Seyler 368:121-30. 1987
    ..Tetragonal and monoclinic crystals of DNA-binding protein II have been obtained which are suitable for X-ray studies and the diffraction patterns of the two crystal forms are shown...
  44. ncbi request reprint New angiographic measurement tool for analysis of small cerebral vessels: application to a subarachnoid haemorrhage model in the rat
    B Turowski
    Institute of Diagnostic Radiology, Neuroradiology, Heinrich Heine University, Moorenstr 5, 40225 Duesselorf, Germany
    Neuroradiology 49:129-37. 2007
    ..The purpose of the study was to develop a new method for computerized analysis of small arteries and to demonstrate feasibility on cerebral angiographies of rats acquired on a clinical angiography unit...
  45. ncbi request reprint FGFR3 and p53 protein expressions in patients with pTa and pT1 urothelial bladder cancer
    P Mhawech-Fauceglia
    Department of Pathology and Laboratory Medicine, Roswell Park Cancer Institute, Elm and Carlton Street, Buffalo, NY 14263, USA
    Eur J Surg Oncol 32:231-7. 2006
    ....
  46. ncbi request reprint Local permutations in the glomerular array of the mouse olfactory bulb
    J Strotmann
    The Rockefeller University, New York, New York 10021, USA
    J Neurosci 20:6927-38. 2000
    ..These local permutations may reflect the developmental history of the glomeruli and are relevant for the construction of spatial odor maps...
  47. ncbi request reprint A novel polyarginine containing Smac peptide conjugate that mediates cell death in tumor and healthy cells
    S Heckl
    Department of Neuroradiology, University of Tubingen, Medical School, Tubingen, Germany
    Med Chem 4:348-54. 2008
    ..This was accompanied by cell death. In contrast to expectations, it appears that the apoptotic effects observed do not result from the SmacN7 cargo alone...
  48. ncbi request reprint A novel bipolar mode of attachment to aluminium-containing adjuvants by BBG2Na, a recombinant subunit hRSV vaccine
    N Dagouassat
    , Centre d'Immunologie Pierre-Fabre, , BP 497, F74164, Saint-Julien-en-Genevois, France
    Vaccine 19:4143-52. 2001
    ..From a physicochemical point of view, this unique property of BBG2Na makes it eminently suitable for combination to either paediatric or elderly multivalent AH- or AP-containing vaccines already in the market or in development...
  49. ncbi request reprint Genetic immunization and comprehensive screening approaches in HLA-A2 transgenic mice lead to the identification of three novel epitopes in hepatitis C virus NS3 antigen
    P Martin
    FRE 2736 CNRS bioMérieux, IFR 128 Biosciences Lyon Gerland, Lyon, France
    J Med Virol 74:397-405. 2004
    ..These data increase the pool of NS3-specific CD8+ T cell epitopes available to analyze HCV associated immunity and could contribute to the design and evaluation of candidate vaccines...
  50. ncbi request reprint Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies
    P Mhawech-Fauceglia
    Department of Pathology and Laboratory Medicine, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Histopathology 49:569-75. 2006
    ....