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Genomes and Genes | S BoiteuxSummaryAffiliation: CEA Saclay Country: France Publications
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Publications
Excision repair of 8-hydroxyguanine in mammalian cells: the mouse Ogg1 protein as a modelS Boiteux
CEA DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
Free Radic Res 29:487-97. 1998..The isolation of this gene will allow the development of an animal model to study the effects of oxidative stress on carcinogenesis and degenerative diseases...- Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylaseFranck Coste
Centre de Biophysique Moleculaire, UPR4301, CNRS, rue Charles Sadron, 45071 Orleans Cedex 02, France
J Biol Chem 279:44074-83. 2004..The significant differences between the Fpg recognition modes of 8-oxodG and FapydG provide new insights into the Fpg substrate specificity... - Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiaeSerge Boiteux
CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS, Radiobiologie Moléculaire et Cellulaire, BP 6, F 92265, Fontenay aux Roses, France
DNA Repair (Amst) 3:1-12. 2004..These results in yeast demonstrate that AP sites are critical endogenous DNA damages that cause genetic instability and by analogy could be associated with degenerative pathologies in human... - Repair of 8-oxoguanine in Saccharomyces cerevisiae: interplay of DNA repair and replication mechanismsSerge Boiteux
CEA, DSV, Departement de Radiobiologie et Radiopathologie, CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
Free Radic Biol Med 32:1244-53. 2002..The aim of this review is to summarize recent literature dealing with the replication and repair of 8-oxoG in Saccharomyces cerevisiae, which can be used as a paradigm for DNA repair in eukaryotes... - The human OGG1 gene: structure, functions, and its implication in the process of carcinogenesisS Boiteux
CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, 92265, France
Arch Biochem Biophys 377:1-8. 2000..A review of the recent literature on the mammalian Ogg1 proteins, the main repair system involved in the elimination of this mutagenic lesion, is presented... 
Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidinesC Dherin
CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA, Radiobiologie Moléculaire et Cellulaire, 60 rue du Général Leclerc, BP6, 92265 Fontenay aux Roses, France
Nucleic Acids Res 28:4583-92. 2000..Therefore, Drosophila possesses two DNA glycosylase activities that can excise 8-OH-Gua and formamidopyrimidines from DNA, dOgg1 and the ribosomal protein S3...- Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiaeJ P Radicella
Laboratoire de Radiobiologie du DNA, Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, UMR217 Centre National de la Recherche Scientifique, 92265 Fontenay aux Roses, France
Proc Natl Acad Sci U S A 94:8010-5. 1997.... - Alterations of the DNA repair gene OGG1 in human clear cell carcinomas of the kidneyM Audebert
Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, Fontenay aux Roses, France
Cancer Res 60:4740-4. 2000..Biochemical analysis of the mutant proteins revealed that a substitution at codon 46 impairs the enzymatic activity. We also describe the occurrence of several polymorphisms as well as aberrantly spliced OGG1 transcripts... - Excision of oxidatively damaged DNA bases by the human alpha-hOgg1 protein and the polymorphic alpha-hOgg1(Ser326Cys) protein which is frequently found in human populationsC Dherin
CEA, DSV, DRR, UMR217 CNRS CEA, Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
Nucleic Acids Res 27:4001-7. 1999..Furthermore, both proteins efficiently complement the mutator phenotype of the fpg mutY mutant of Escherichia coli... - XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactionsA E Vidal
Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR217 CNRS CEA, BP6, F 92265 Fontenay aux Roses, France
EMBO J 20:6530-9. 2001..The interaction described extends the coordinating role of XRCC1 to the initial step of the repair of DNA abasic sites... - Synergism between base excision repair, mediated by the DNA glycosylases Ntg1 and Ntg2, and nucleotide excision repair in the removal of oxidatively damaged DNA bases in Saccharomyces cerevisiaeL Gellon
CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
Mol Genet Genomics 265:1087-96. 2001.... - Excision by the human methylpurine DNA N-glycosylase of cyanuric acid, a stable and mutagenic oxidation product of 8-oxo-7,8-dihydroguanineC Dherin
CEA, , , BP6, F92265 Fontenay aux Roses, France
Int J Radiat Biol 80:21-7. 2004..Most of them, Sp, Gh, Oa and Oz, are substrates of the oxidized bases-specific enzymes such as Nth or Fpg. In contrast, Ca is substrate of the human methylpurine DNA N-glycosylase (Mpg)... - A novel 3-methyladenine DNA glycosylase from Helicobacter pylori defines a new class within the endonuclease III family of base excision repair glycosylasesE J O'Rourke
Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR217 CEA CNRS, BP6, 92265 Fontenay aux Roses, France
J Biol Chem 275:20077-83. 2000..MagIII is thus a novel bacterial member of the endonuclease III family, which displays biochemical properties not described for any of the members of this group until now... - Mutations in OGG1, a gene involved in the repair of oxidative DNA damage, are found in human lung and kidney tumoursS Chevillard
Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
Oncogene 16:3083-6. 1998..For the mutant kidney tumour, the normal tissue counterpart shows a wild-type profile. These results suggest a role for OGG1 mutations in the course of the multistage process of carcinogenesis in lung or kidney... - Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiaeM Padula
Laboratoire de Radiobiologie de l ADN, Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, Centre National de la Recherche Scientifique, France
Braz J Med Biol Res 32:1063-71. 1999..Moreover, nucleotide excision repair seems to play a major role in the recognition and repair of these lesions in Saccharomyces cerevisiae... - [Repair of oxidized guanine in mammals: OGG1 genes]J P Radicella
Laboratoire de Radiobiologie de l ADN, UMR217 CNRS CEA, Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, Fontenay aux Roses, France
C R Seances Soc Biol Fil 191:755-63. 1997..The localization of this gene to chromosome 3p and other evidences discussed in this paper indicate that OGG1 could be a tumor suppressor gene implicated in lung cancer... - Ultraviolet-B-induced inactivation of human OGG1, the repair enzyme for removal of 8-oxoguanine in DNAP Auffret van der Kemp
CEA, DSV, , , Fontenay aux Roses, France
Photochem Photobiol 76:640-8. 2002..Besides the role of Trp residues, the possible involvement of Cys 253 in the photoinactivation process of hOGG1 is discussed... - Use of yeast for detection of endogenous abasic lesions, their source, and their repairSerge Boiteux
Laboratory of Radiobiology DNA, Department of Radiobiology and Radiopathology, Aus Roses, France
Methods Enzymol 408:79-91. 2006..A model that summarizes our present and puzzling data on the origin and repair of endogenous AP sites is also presented... - Catalytic and DNA-binding properties of the human Ogg1 DNA N-glycosylase/AP lyase: biochemical exploration of H270, Q315 and F319, three amino acids of the 8-oxoguanine-binding pocketPatricia Auffret van der Kemp
CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP 6, F 92265 Fontenay aux Roses, France
Nucleic Acids Res 32:570-8. 2004..C and cleavage of AP sites. Finally, hOgg1 mutant proteins with a substitution of H270A or F319A are members of a new type of hOgg1 that is deficient in DNA glycosylase but proficient in AP lyase... - The post-replication repair RAD18 and RAD6 genes are involved in the prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiaeMarcelo de Padula
CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP6, 92265 Fontenay aux Roses, France
Nucleic Acids Res 32:5003-10. 2004..Our current model suggests that the Rad6-Rad18 complex targets Poleta at DNA gaps that result from the MMR-mediated excision of adenine mispaired with 8-oxoG, allowing error-free dCMP incorporation opposite to this lesion... - Analysis of 8-hydroxyguanine (8-OH-Gua) released from DNA by the formamidopyrimidine DNA glycosylase (Fpg) protein: a reliable method to estimate cellular oxidative stressHiroshi Orimo
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
J Radiat Res (Tokyo) 45:455-60. 2004..With our new analysis method, it is possible to detect the small changes in the 8-OH-Gua levels in cellular DNA induced by various environmental factors... - Trapping of DNA topoisomerase I on nick-containing DNA in cell free extracts of Saccharomyces cerevisiaeNatalia Lebedeva
CEA, , Route du Panorama, BP6, 92265-Fontenay aux Roses, France
DNA Repair (Amst) 5:799-809. 2006..Since nicked DNA structures are frequently formed in the course of BER, their covalent linkage to Top1 has the potential to interfere with BER in vivo... - dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiaeMarie Guillet
CEA, DSV Département de Radiobiologie et Radiopathologie, UMR 217 CNRS Radiobiologie Moléculaire et Cellulaire, BP 6, 92265 Fontenay aux Roses, France
Nucleic Acids Res 34:2056-66. 2006..Therefore, the normal cellular metabolism, and not only its byproducts, is an important source of endogenous DNA damage and genetic instability in eukaryotic cells... - Origin of endogenous DNA abasic sites in Saccharomyces cerevisiaeMarie Guillet
CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, F 92265 Fontenay aux Roses, France
Mol Cell Biol 23:8386-94. 2003..Therefore, this result points to the dUTP pool as an important source of the formation of endogenous AP sites in eukaryotes... - Role of XRCC1 in the coordination and stimulation of oxidative DNA damage repair initiated by the DNA glycosylase hOGG1Stéphanie Marsin
Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS, Commissariat a l Energie Atomique, BP6, F 92265 Fontenay aux Roses, France
J Biol Chem 278:44068-74. 2003.... - Ntg2p, a Saccharomyces cerevisiae DNA N-glycosylase/apurinic or apyrimidinic lyase involved in base excision repair of oxidative DNA damage, interacts with the DNA mismatch repair protein Mlh1p. Identification of a Mlh1p binding motifLionel Gellon
Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses 92265, France
J Biol Chem 277:29963-72. 2002..Therefore, we propose that the R/K-S-R/K-Y/F-Y/F sequence could define a Mhl1 binding motif. The results also suggest that base excision repair and MMR can cooperate to prevent deleterious effects of oxidative DNA damage... - Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNALaurence Serre
Institut de Biologie Structurale, CNRS CEA, 41 av Jules Horowitz, 38027 Grenoble Cedex 01, France
EMBO J 21:2854-65. 2002..In addition, the modelling of the 8-oxoguanine residue allows us to define an enzyme pocket that may accommodate the extrahelical oxidized base... - A global DNA repair mechanism involving the Cockayne syndrome B (CSB) gene product can prevent the in vivo accumulation of endogenous oxidative DNA base damageMarcel Osterod
Institute of Pharmacy, University of Mainz, D 55099 Mainz, Germany
Oncogene 21:8232-9. 2002..The data indicate a role for Csb in the removal of 8-oxoG from the overall genome that is independent of both Ogg1-mediated base excision repair and regular transcription... - Mitochondrial targeting of human 8-oxoguanine DNA glycosylase hOGG1 is impaired by a somatic mutation found in kidney cancerMarc Audebert
Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA, Commissariat a l Energie Atomique, BP6, F 92265 Fontenay aux Roses, France
DNA Repair (Amst) 1:497-505. 2002..These results imply that these cancer cells are deficient in their mitochondrial 8-oxoG DNA repair activity... - Excision of 8-methylguanine site-specifically incorporated into oligonucleotide substrates by the AlkA protein of Escherichia coliDidier Gasparutto
, Service de Chimie Inorganique et Biologique, UMR 5046 CEA-CNRS-UJF, , CEA-Grenoble, 17 Avenue des Martyrs F-38054 Cedex 9, Grenoble, France
DNA Repair (Amst) 1:437-47. 2002.... - 32nd annual meeting of European Environmental Mutagen Society. DNA damage and repair fundamental aspects and contribution to human disordersBarbara Tudek
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland
DNA Repair (Amst) 2:765-81. 2003 - Endogenous DNA abasic sites cause cell death in the absence of Apn1, Apn2 and Rad1/Rad10 in Saccharomyces cerevisiaeMarie Guillet
CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP6, F 92265 Fontenay aux Roses, France
EMBO J 21:2833-41. 2002..Therefore, we suggest that the essential and overlapping function of Apn1, Apn2, Rad1/Rad10 and Mus81/Mms4 is to repair 3'-blocked SSBs using their 3'-phosphodiesterase activity or their 3'-flap endonuclease activity, respectively... - A mutation in EXO1 defines separable roles in DNA mismatch repair and post-replication repairPhuoc T Tran
Department of Radiation Oncology, Stanford Hospital and Clinics, Stanford, CA 94305, USA
DNA Repair (Amst) 6:1572-83. 2007..Lastly, by using a compound exo1 mutant that was defective for interaction with Mlh1p and deficient for nuclease activity, we provide further evidence that Exo1p plays both structural and catalytic roles during MMR... 
Trapping of human DNA topoisomerase I by DNA structures mimicking intermediates of DNA repairNatalia Lebedeva
Institute of Chemical Biology and Fundamental Medicine, Prospect Lavrentieva 8, 630090, Novosibirsk, Russia
IUBMB Life 60:130-4. 2008..Top1-DNA covalent adducts formed in cells exposed to DNA damaging agents can promote genetic instability...- XRCC1 interactions with multiple DNA glycosylases: a model for its recruitment to base excision repairAnna Campalans
Departement de Radiobiologie et Radiopathologie, CEA, UMR217 CNRS CEA, 18 route du Panorama, F 92265 Fontenay aux Roses, France
DNA Repair (Amst) 4:826-35. 2005..XRCC1 would then coordinate the subsequent enzymatic steps and modulate the activities of all the proteins involved... - Poly(ADP-ribose) polymerase-1 (PARP-1) is required in murine cell lines for base excision repair of oxidative DNA damage in the absence of DNA polymerase betaFlorence Le Page
Commissariat a l Energie Atomique CEA, Direction des Sciences du Vivant, Departement de Radiobiologie et Radiopathologie, Unité Mixte de Recherche 217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, 92265 Fontenay aux Roses, France
J Biol Chem 278:18471-7. 2003.... - Yeast genes involved in cadmium tolerance: Identification of DNA replication as a target of cadmium toxicityAlexandre Serero
Commissariat a l Energie Atomique, IRCM, Laboratoire de Radiobiologie de l ADN, CNRS UMR 217, Fontenay aux Roses, France
DNA Repair (Amst) 7:1262-75. 2008..Taken together these results provide a global picture of the genetic requirement for cadmium tolerance in yeast and strongly suggest that DNA replication, through the step of Okazaki fragment processing, is a target of cadmium toxicity...