S Boiteux

Summary

Affiliation: CEA Saclay
Country: France

Publications

  1. ncbi Excision repair of 8-hydroxyguanine in mammalian cells: the mouse Ogg1 protein as a model
    S Boiteux
    CEA DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Free Radic Res 29:487-97. 1998
  2. ncbi Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase
    Franck Coste
    Centre de Biophysique Moleculaire, UPR4301, CNRS, rue Charles Sadron, 45071 Orleans Cedex 02, France
    J Biol Chem 279:44074-83. 2004
  3. ncbi Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiae
    Serge Boiteux
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS, Radiobiologie Moléculaire et Cellulaire, BP 6, F 92265, Fontenay aux Roses, France
    DNA Repair (Amst) 3:1-12. 2004
  4. ncbi Repair of 8-oxoguanine in Saccharomyces cerevisiae: interplay of DNA repair and replication mechanisms
    Serge Boiteux
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Free Radic Biol Med 32:1244-53. 2002
  5. ncbi The human OGG1 gene: structure, functions, and its implication in the process of carcinogenesis
    S Boiteux
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, 92265, France
    Arch Biochem Biophys 377:1-8. 2000
  6. pmc Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines
    C Dherin
    CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA, Radiobiologie Moléculaire et Cellulaire, 60 rue du Général Leclerc, BP6, 92265 Fontenay aux Roses, France
    Nucleic Acids Res 28:4583-92. 2000
  7. pmc Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae
    J P Radicella
    Laboratoire de Radiobiologie du DNA, Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, UMR217 Centre National de la Recherche Scientifique, 92265 Fontenay aux Roses, France
    Proc Natl Acad Sci U S A 94:8010-5. 1997
  8. ncbi Alterations of the DNA repair gene OGG1 in human clear cell carcinomas of the kidney
    M Audebert
    Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, Fontenay aux Roses, France
    Cancer Res 60:4740-4. 2000
  9. pmc Excision of oxidatively damaged DNA bases by the human alpha-hOgg1 protein and the polymorphic alpha-hOgg1(Ser326Cys) protein which is frequently found in human populations
    C Dherin
    CEA, DSV, DRR, UMR217 CNRS CEA, Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Nucleic Acids Res 27:4001-7. 1999
  10. pmc XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions
    A E Vidal
    Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR217 CNRS CEA, BP6, F 92265 Fontenay aux Roses, France
    EMBO J 20:6530-9. 2001

Collaborators

Detail Information

Publications37

  1. ncbi Excision repair of 8-hydroxyguanine in mammalian cells: the mouse Ogg1 protein as a model
    S Boiteux
    CEA DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Free Radic Res 29:487-97. 1998
    ..The isolation of this gene will allow the development of an animal model to study the effects of oxidative stress on carcinogenesis and degenerative diseases...
  2. ncbi Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase
    Franck Coste
    Centre de Biophysique Moleculaire, UPR4301, CNRS, rue Charles Sadron, 45071 Orleans Cedex 02, France
    J Biol Chem 279:44074-83. 2004
    ..The significant differences between the Fpg recognition modes of 8-oxodG and FapydG provide new insights into the Fpg substrate specificity...
  3. ncbi Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiae
    Serge Boiteux
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS, Radiobiologie Moléculaire et Cellulaire, BP 6, F 92265, Fontenay aux Roses, France
    DNA Repair (Amst) 3:1-12. 2004
    ..These results in yeast demonstrate that AP sites are critical endogenous DNA damages that cause genetic instability and by analogy could be associated with degenerative pathologies in human...
  4. ncbi Repair of 8-oxoguanine in Saccharomyces cerevisiae: interplay of DNA repair and replication mechanisms
    Serge Boiteux
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Free Radic Biol Med 32:1244-53. 2002
    ..The aim of this review is to summarize recent literature dealing with the replication and repair of 8-oxoG in Saccharomyces cerevisiae, which can be used as a paradigm for DNA repair in eukaryotes...
  5. ncbi The human OGG1 gene: structure, functions, and its implication in the process of carcinogenesis
    S Boiteux
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, 92265, France
    Arch Biochem Biophys 377:1-8. 2000
    ..A review of the recent literature on the mammalian Ogg1 proteins, the main repair system involved in the elimination of this mutagenic lesion, is presented...
  6. pmc Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines
    C Dherin
    CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA, Radiobiologie Moléculaire et Cellulaire, 60 rue du Général Leclerc, BP6, 92265 Fontenay aux Roses, France
    Nucleic Acids Res 28:4583-92. 2000
    ..Therefore, Drosophila possesses two DNA glycosylase activities that can excise 8-OH-Gua and formamidopyrimidines from DNA, dOgg1 and the ribosomal protein S3...
  7. pmc Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae
    J P Radicella
    Laboratoire de Radiobiologie du DNA, Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, UMR217 Centre National de la Recherche Scientifique, 92265 Fontenay aux Roses, France
    Proc Natl Acad Sci U S A 94:8010-5. 1997
    ....
  8. ncbi Alterations of the DNA repair gene OGG1 in human clear cell carcinomas of the kidney
    M Audebert
    Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, Fontenay aux Roses, France
    Cancer Res 60:4740-4. 2000
    ..Biochemical analysis of the mutant proteins revealed that a substitution at codon 46 impairs the enzymatic activity. We also describe the occurrence of several polymorphisms as well as aberrantly spliced OGG1 transcripts...
  9. pmc Excision of oxidatively damaged DNA bases by the human alpha-hOgg1 protein and the polymorphic alpha-hOgg1(Ser326Cys) protein which is frequently found in human populations
    C Dherin
    CEA, DSV, DRR, UMR217 CNRS CEA, Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Nucleic Acids Res 27:4001-7. 1999
    ..Furthermore, both proteins efficiently complement the mutator phenotype of the fpg mutY mutant of Escherichia coli...
  10. pmc XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions
    A E Vidal
    Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR217 CNRS CEA, BP6, F 92265 Fontenay aux Roses, France
    EMBO J 20:6530-9. 2001
    ..The interaction described extends the coordinating role of XRCC1 to the initial step of the repair of DNA abasic sites...
  11. ncbi Synergism between base excision repair, mediated by the DNA glycosylases Ntg1 and Ntg2, and nucleotide excision repair in the removal of oxidatively damaged DNA bases in Saccharomyces cerevisiae
    L Gellon
    CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Mol Genet Genomics 265:1087-96. 2001
    ....
  12. ncbi Excision by the human methylpurine DNA N-glycosylase of cyanuric acid, a stable and mutagenic oxidation product of 8-oxo-7,8-dihydroguanine
    C Dherin
    CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, BP6, F92265 Fontenay aux Roses, France
    Int J Radiat Biol 80:21-7. 2004
    ..A transversions like 8-oxoG. These results call attention to the potential importance of secondary oxidation products of 8-oxoG. The present study investigates the capability of several DNA N-glycosylases to remove the Ca lesion in DNA...
  13. ncbi A novel 3-methyladenine DNA glycosylase from Helicobacter pylori defines a new class within the endonuclease III family of base excision repair glycosylases
    E J O'Rourke
    Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR217 CEA CNRS, BP6, 92265 Fontenay aux Roses, France
    J Biol Chem 275:20077-83. 2000
    ..MagIII is thus a novel bacterial member of the endonuclease III family, which displays biochemical properties not described for any of the members of this group until now...
  14. ncbi Mutations in OGG1, a gene involved in the repair of oxidative DNA damage, are found in human lung and kidney tumours
    S Chevillard
    Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Oncogene 16:3083-6. 1998
    ..For the mutant kidney tumour, the normal tissue counterpart shows a wild-type profile. These results suggest a role for OGG1 mutations in the course of the multistage process of carcinogenesis in lung or kidney...
  15. ncbi Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
    M Padula
    Laboratoire de Radiobiologie de l ADN, Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, Centre National de la Recherche Scientifique, France
    Braz J Med Biol Res 32:1063-71. 1999
    ..Moreover, nucleotide excision repair seems to play a major role in the recognition and repair of these lesions in Saccharomyces cerevisiae...
  16. ncbi [Repair of oxidized guanine in mammals: OGG1 genes]
    J P Radicella
    Laboratoire de Radiobiologie de l ADN, UMR217 CNRS CEA, Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, Fontenay aux Roses, France
    C R Seances Soc Biol Fil 191:755-63. 1997
    ..The localization of this gene to chromosome 3p and other evidences discussed in this paper indicate that OGG1 could be a tumor suppressor gene implicated in lung cancer...
  17. ncbi Ultraviolet-B-induced inactivation of human OGG1, the repair enzyme for removal of 8-oxoguanine in DNA
    P Auffret van der Kemp
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses, France
    Photochem Photobiol 76:640-8. 2002
    ..Besides the role of Trp residues, the possible involvement of Cys 253 in the photoinactivation process of hOGG1 is discussed...
  18. ncbi Use of yeast for detection of endogenous abasic lesions, their source, and their repair
    Serge Boiteux
    Laboratory of Radiobiology DNA, Department of Radiobiology and Radiopathology, Aus Roses, France
    Methods Enzymol 408:79-91. 2006
    ..A model that summarizes our present and puzzling data on the origin and repair of endogenous AP sites is also presented...
  19. pmc Catalytic and DNA-binding properties of the human Ogg1 DNA N-glycosylase/AP lyase: biochemical exploration of H270, Q315 and F319, three amino acids of the 8-oxoguanine-binding pocket
    Patricia Auffret van der Kemp
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP 6, F 92265 Fontenay aux Roses, France
    Nucleic Acids Res 32:570-8. 2004
    ..C and cleavage of AP sites. Finally, hOgg1 mutant proteins with a substitution of H270A or F319A are members of a new type of hOgg1 that is deficient in DNA glycosylase but proficient in AP lyase...
  20. pmc The post-replication repair RAD18 and RAD6 genes are involved in the prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiae
    Marcelo de Padula
    CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP6, 92265 Fontenay aux Roses, France
    Nucleic Acids Res 32:5003-10. 2004
    ..Our current model suggests that the Rad6-Rad18 complex targets Poleta at DNA gaps that result from the MMR-mediated excision of adenine mispaired with 8-oxoG, allowing error-free dCMP incorporation opposite to this lesion...
  21. ncbi Analysis of 8-hydroxyguanine (8-OH-Gua) released from DNA by the formamidopyrimidine DNA glycosylase (Fpg) protein: a reliable method to estimate cellular oxidative stress
    Hiroshi Orimo
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    J Radiat Res 45:455-60. 2004
    ..With our new analysis method, it is possible to detect the small changes in the 8-OH-Gua levels in cellular DNA induced by various environmental factors...
  22. ncbi Trapping of DNA topoisomerase I on nick-containing DNA in cell free extracts of Saccharomyces cerevisiae
    Natalia Lebedeva
    CEA, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, Route du Panorama, BP6, 92265 Fontenay aux Roses, France
    DNA Repair (Amst) 5:799-809. 2006
    ..Since nicked DNA structures are frequently formed in the course of BER, their covalent linkage to Top1 has the potential to interfere with BER in vivo...
  23. pmc dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae
    Marie Guillet
    CEA, DSV Département de Radiobiologie et Radiopathologie, UMR 217 CNRS Radiobiologie Moléculaire et Cellulaire, BP 6, 92265 Fontenay aux Roses, France
    Nucleic Acids Res 34:2056-66. 2006
    ..Therefore, the normal cellular metabolism, and not only its byproducts, is an important source of endogenous DNA damage and genetic instability in eukaryotic cells...
  24. pmc Origin of endogenous DNA abasic sites in Saccharomyces cerevisiae
    Marie Guillet
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, F 92265 Fontenay aux Roses, France
    Mol Cell Biol 23:8386-94. 2003
    ..Therefore, this result points to the dUTP pool as an important source of the formation of endogenous AP sites in eukaryotes...
  25. ncbi Role of XRCC1 in the coordination and stimulation of oxidative DNA damage repair initiated by the DNA glycosylase hOGG1
    Stéphanie Marsin
    Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS, Commissariat a l Energie Atomique, BP6, F 92265 Fontenay aux Roses, France
    J Biol Chem 278:44068-74. 2003
    ....
  26. ncbi Ntg2p, a Saccharomyces cerevisiae DNA N-glycosylase/apurinic or apyrimidinic lyase involved in base excision repair of oxidative DNA damage, interacts with the DNA mismatch repair protein Mlh1p. Identification of a Mlh1p binding motif
    Lionel Gellon
    Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, Fontenay aux Roses 92265, France
    J Biol Chem 277:29963-72. 2002
    ..Therefore, we propose that the R/K-S-R/K-Y/F-Y/F sequence could define a Mhl1 binding motif. The results also suggest that base excision repair and MMR can cooperate to prevent deleterious effects of oxidative DNA damage...
  27. pmc Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA
    Laurence Serre
    Institut de Biologie Structurale, CNRS CEA, 41 av Jules Horowitz, 38027 Grenoble Cedex 01, France
    EMBO J 21:2854-65. 2002
    ..In addition, the modelling of the 8-oxoguanine residue allows us to define an enzyme pocket that may accommodate the extrahelical oxidized base...
  28. ncbi A global DNA repair mechanism involving the Cockayne syndrome B (CSB) gene product can prevent the in vivo accumulation of endogenous oxidative DNA base damage
    Marcel Osterod
    Institute of Pharmacy, University of Mainz, D 55099 Mainz, Germany
    Oncogene 21:8232-9. 2002
    ..The data indicate a role for Csb in the removal of 8-oxoG from the overall genome that is independent of both Ogg1-mediated base excision repair and regular transcription...
  29. ncbi Mitochondrial targeting of human 8-oxoguanine DNA glycosylase hOGG1 is impaired by a somatic mutation found in kidney cancer
    Marc Audebert
    Departement de Radiobiologie et Radiopathologie, UMR217 CNRS CEA, Commissariat a l Energie Atomique, BP6, F 92265 Fontenay aux Roses, France
    DNA Repair (Amst) 1:497-505. 2002
    ..These results imply that these cancer cells are deficient in their mitochondrial 8-oxoG DNA repair activity...
  30. ncbi Excision of 8-methylguanine site-specifically incorporated into oligonucleotide substrates by the AlkA protein of Escherichia coli
    Didier Gasparutto
    Laboratoire des Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique, UMR 5046 CEA CNRS UJF, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, 17 Avenue des Martyrs F 38054 Cedex 9, Grenoble, France
    DNA Repair (Amst) 1:437-47. 2002
    ....
  31. ncbi 32nd annual meeting of European Environmental Mutagen Society. DNA damage and repair fundamental aspects and contribution to human disorders
    Barbara Tudek
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    DNA Repair (Amst) 2:765-81. 2003
  32. pmc Endogenous DNA abasic sites cause cell death in the absence of Apn1, Apn2 and Rad1/Rad10 in Saccharomyces cerevisiae
    Marie Guillet
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP6, F 92265 Fontenay aux Roses, France
    EMBO J 21:2833-41. 2002
    ..Therefore, we suggest that the essential and overlapping function of Apn1, Apn2, Rad1/Rad10 and Mus81/Mms4 is to repair 3'-blocked SSBs using their 3'-phosphodiesterase activity or their 3'-flap endonuclease activity, respectively...
  33. pmc A mutation in EXO1 defines separable roles in DNA mismatch repair and post-replication repair
    Phuoc T Tran
    Department of Radiation Oncology, Stanford Hospital and Clinics, Stanford, CA 94305, USA
    DNA Repair (Amst) 6:1572-83. 2007
    ..Lastly, by using a compound exo1 mutant that was defective for interaction with Mlh1p and deficient for nuclease activity, we provide further evidence that Exo1p plays both structural and catalytic roles during MMR...
  34. doi Trapping of human DNA topoisomerase I by DNA structures mimicking intermediates of DNA repair
    Natalia Lebedeva
    Institute of Chemical Biology and Fundamental Medicine, Prospect Lavrentieva 8, 630090, Novosibirsk, Russia
    IUBMB Life 60:130-4. 2008
    ..Top1-DNA covalent adducts formed in cells exposed to DNA damaging agents can promote genetic instability...
  35. ncbi XRCC1 interactions with multiple DNA glycosylases: a model for its recruitment to base excision repair
    Anna Campalans
    Departement de Radiobiologie et Radiopathologie, CEA, UMR217 CNRS CEA, 18 route du Panorama, F 92265 Fontenay aux Roses, France
    DNA Repair (Amst) 4:826-35. 2005
    ..XRCC1 would then coordinate the subsequent enzymatic steps and modulate the activities of all the proteins involved...
  36. ncbi Poly(ADP-ribose) polymerase-1 (PARP-1) is required in murine cell lines for base excision repair of oxidative DNA damage in the absence of DNA polymerase beta
    Florence Le Page
    Commissariat a l Energie Atomique CEA, Direction des Sciences du Vivant, Departement de Radiobiologie et Radiopathologie, Unité Mixte de Recherche 217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, 92265 Fontenay aux Roses, France
    J Biol Chem 278:18471-7. 2003
    ....
  37. doi Yeast genes involved in cadmium tolerance: Identification of DNA replication as a target of cadmium toxicity
    Alexandre Serero
    Commissariat a l Energie Atomique, IRCM, Laboratoire de Radiobiologie de l ADN, CNRS UMR 217, Fontenay aux Roses, France
    DNA Repair (Amst) 7:1262-75. 2008
    ..Taken together these results provide a global picture of the genetic requirement for cadmium tolerance in yeast and strongly suggest that DNA replication, through the step of Okazaki fragment processing, is a target of cadmium toxicity...