Jean Cadet

Summary

Country: France

Publications

  1. ncbi Oxidative damage to DNA: formation, measurement and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC and FRE 2600, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 531:5-23. 2003
  2. ncbi Radiation-induced DNA damage: formation, measurement, and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC, CEA Grenoble, France
    J Environ Pathol Toxicol Oncol 23:33-43. 2004
  3. ncbi Ultraviolet radiation-mediated damage to cellular DNA
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, Grenoble Cedex 9 F 38054, France
    Mutat Res 571:3-17. 2005
  4. ncbi Assessment of oxidative base damage to isolated and cellular DNA by HPLC-MS/MS measurement
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, Grenoble, France
    Free Radic Biol Med 33:441-9. 2002
  5. ncbi Radiation-induced formation of purine 5',8-cyclonucleosides in isolated and cellular DNA: high stereospecificity and modulating effect of oxygen
    Nourreddine Belmadoui
    Laboratoire Lesions des Acides Nucleiques, DSM INAC SCIB UMR E 3 CEA UJF and FRE CNRS 3200CEA Grenoble, 38054 Grenoble Cedex 9, France
    Org Biomol Chem 8:3211-9. 2010
  6. ncbi Guanine-thymine intrastrand cross-linked lesion containing oligonucleotides: from chemical synthesis to in vitro enzymatic replication
    Sophie Bellon
    Service de Chimie Inorganique et Biologique UMR E3 CEA UJF, , CEA Grenoble, 17 Avenue des Martyrs, F-38054, Grenoble Cedex 9, France
    Org Biomol Chem 4:3831-7. 2006
  7. ncbi Excision of the oxidatively formed 5-hydroxyhydantoin and 5-hydroxy-5-methylhydantoin pyrimidine lesions by Escherichia coli and Saccharomyces cerevisiae DNA N-glycosylases
    Didier Gasparutto
    Laboratoire des Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique UMR E3 CEA UJF, INAC, DSM, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Biochim Biophys Acta 1790:16-24. 2009
  8. ncbi Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation
    Stéphane Mouret
    Commissariat à l Energie Atomique CEA La Direction des Sciences de la Matière DSM Département de Recherche Fondamentale sur la Matière Condensée, Service de Chimie Inorganique et Biologique UMR E 3 CEA UJF, CEA Grenoble, France
    Proc Natl Acad Sci U S A 103:13765-70. 2006
  9. ncbi Enhancement of the in vitro transcription by T7 RNA polymerase of short DNA templates containing oxidative thymine lesions
    Valérie Guerniou
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, FRE 2600, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 17, rue des Martyrs, 38054 Grenoble Cedex 9, France
    C R Biol 328:794-801. 2005
  10. ncbi Differential repair of UVB-induced cyclobutane pyrimidine dimers in cultured human skin cells and whole human skin
    Stéphane Mouret
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique UMR E 3 CEA UJF, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 38054 Grenoble Cedex 9, France
    DNA Repair (Amst) 7:704-12. 2008

Collaborators

Detail Information

Publications79

  1. ncbi Oxidative damage to DNA: formation, measurement and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC and FRE 2600, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 531:5-23. 2003
    ..For this purpose modified oligonucleotides in which cyclopurine, and cyclopyrimidine nucleosides were site-specifically inserted were synthesized...
  2. ncbi Radiation-induced DNA damage: formation, measurement, and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC, CEA Grenoble, France
    J Environ Pathol Toxicol Oncol 23:33-43. 2004
    ..Information is also provided on the substrate specificity of DNA repair enzymes and the mutagenic potential of base lesions using site-specific modified oligonucleotides as the probes...
  3. ncbi Ultraviolet radiation-mediated damage to cellular DNA
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, Grenoble Cedex 9 F 38054, France
    Mutat Res 571:3-17. 2005
    ....
  4. ncbi Assessment of oxidative base damage to isolated and cellular DNA by HPLC-MS/MS measurement
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, Grenoble, France
    Free Radic Biol Med 33:441-9. 2002
    ..This explains why the combined use of DNA repair glycosylases with either the comet assay or the alkaline elution technique is a better alternative to monitor the formation of low levels of oxidized bases within cellular DNA...
  5. ncbi Radiation-induced formation of purine 5',8-cyclonucleosides in isolated and cellular DNA: high stereospecificity and modulating effect of oxygen
    Nourreddine Belmadoui
    Laboratoire Lesions des Acides Nucleiques, DSM INAC SCIB UMR E 3 CEA UJF and FRE CNRS 3200CEA Grenoble, 38054 Grenoble Cedex 9, France
    Org Biomol Chem 8:3211-9. 2010
    ....
  6. ncbi Guanine-thymine intrastrand cross-linked lesion containing oligonucleotides: from chemical synthesis to in vitro enzymatic replication
    Sophie Bellon
    Service de Chimie Inorganique et Biologique UMR E3 CEA UJF, , CEA Grenoble, 17 Avenue des Martyrs, F-38054, Grenoble Cedex 9, France
    Org Biomol Chem 4:3831-7. 2006
    ..These results are strongly suggestive that the present intrastrand cross-link lesion, if not repaired, would constitute a blocking lesion for prokaryotic DNA polymerases, being likely lethal for the cell...
  7. ncbi Excision of the oxidatively formed 5-hydroxyhydantoin and 5-hydroxy-5-methylhydantoin pyrimidine lesions by Escherichia coli and Saccharomyces cerevisiae DNA N-glycosylases
    Didier Gasparutto
    Laboratoire des Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique UMR E3 CEA UJF, INAC, DSM, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Biochim Biophys Acta 1790:16-24. 2009
    ..If not repaired, when present in cellular DNA, these base lesions may be processed by DNA polymerases that induce mutagenic and cell lethality processes...
  8. ncbi Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation
    Stéphane Mouret
    Commissariat à l Energie Atomique CEA La Direction des Sciences de la Matière DSM Département de Recherche Fondamentale sur la Matière Condensée, Service de Chimie Inorganique et Biologique UMR E 3 CEA UJF, CEA Grenoble, France
    Proc Natl Acad Sci U S A 103:13765-70. 2006
    ..These observations emphasize the likely role played by the UVA-induced DNA damage in skin carcinogenesis and should have consequences for photoprotection strategies...
  9. ncbi Enhancement of the in vitro transcription by T7 RNA polymerase of short DNA templates containing oxidative thymine lesions
    Valérie Guerniou
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, FRE 2600, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 17, rue des Martyrs, 38054 Grenoble Cedex 9, France
    C R Biol 328:794-801. 2005
    ....
  10. ncbi Differential repair of UVB-induced cyclobutane pyrimidine dimers in cultured human skin cells and whole human skin
    Stéphane Mouret
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique UMR E 3 CEA UJF, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 38054 Grenoble Cedex 9, France
    DNA Repair (Amst) 7:704-12. 2008
    ..A noticeable exception concerns CC dinucleotides that are mutational hotspots with an UV-specific CC to TT tandem mutation, although related bipyrimidine photoproducts are produced in low yields and efficiently repaired...
  11. ncbi Singlet oxygen oxidation of isolated and cellular DNA: product formation and mechanistic insights
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, DRFMC SCIB UMR E No 3 CEA UJF, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Photochem Photobiol 82:1219-25. 2006
    ....
  12. ncbi Unrepaired cyclobutane pyrimidine dimers do not prevent proliferation of UV-B-irradiated cultured human fibroblasts
    Sophie Courdavault
    , Service de Chimie Inorganique et Biologique, , CEA-Grenoble, France
    Photochem Photobiol 79:145-51. 2004
    ..These observations emphasize the role of CPD in the mutagenic effects of UV-B...
  13. ncbi Minor contribution of direct ionization to DNA base damage inducedby heavy ions
    Thierry Douki
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique UMR E 3 CEA UJF, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, Grenoble, France
    Int J Radiat Biol 82:119-27. 2006
    ..Emphasis was placed on the estimation of the respective contribution of direct ionization and indirect effects, mostly by comparison with low linear energy transfer (LET) gamma-rays...
  14. ncbi Formation of cross-linked adducts between guanine and thymine mediated by hydroxyl radical and one-electron oxidation: a theoretical study
    Vanessa Labet
    Laboratoire Lesions des Acides Nucleiques, INAC SCIB, UMR E 3 CEA UJF, CEA Grenoble, 17 rue des Martyrs, 38054, Grenoble Cedex 9, France
    Org Biomol Chem 6:3300-5. 2008
    ..Moreover, use of conceptual DFT tools allows the rationalization of the preferential occurrence of G--T and T--G tandem lesions in place of their A--T and T--A counterparts...
  15. ncbi Cellular background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up
    Jean Luc Ravanat
    Laboratoire Lesions des Acides Nucleiques, DRFMC SCIB UMR 5046 CEA Grenoble, 17 Avenue des Martyrs, F 38054 Grenoble Cedex 9, France
    Carcinogenesis 23:1911-8. 2002
    ..Using the best-studied protocol, the steady-state cellular background level of 8-oxodGuo, in a lymphocyte cell line, was determined to be approximately 0.5 lesions/10(6) DNA nucleosides...
  16. ncbi Hydrolytic deamination of 5,6-dihydrocytosine in a protic medium: a theoretical study
    Vanessa Labet
    Laboratoire Lesions des Acides Nucleiques, INAC SCIB UMR E no 3 CEA UJF, CEA Grenoble, 17 rue des Martyrs, F 38 054 Grenoble Cedex 9, France
    J Phys Chem A 114:1826-34. 2010
    ..The evaluation of reactivity indices derived from conceptual DFT leads to the conclusion that this lower activation free energy can be attributed to a larger local electrophilic power of carbon C4 in 5,6-saturated derivatives...
  17. ncbi Predominance of the 1,N2-propano 2'-deoxyguanosine adduct among 4-hydroxy-2-nonenal-induced DNA lesions
    Thierry Douki
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 38054 Grenoble Cedex 9, France
    Free Radic Biol Med 37:62-70. 2004
    ..Altogether, these results suggest than HNE-dGuo may represent the best biomarker of the genotoxic effects of HNE...
  18. ncbi Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation
    Thierry Douki
    , Service de Chimie Inorganique et Biologique and CNRS FRE 2600, , CEA Grenoble, F-38054 Grenoble Cedex 9, France
    Biochemistry 42:9221-6. 2003
    ..Altogether, our data shed new light on the deleterious effect of UVA...
  19. ncbi Formation of 2'-deoxyuridine hydrates upon exposure of nucleosides to gamma radiation and UVC-irradiation of isolated and cellular DNA
    Thierry Douki
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, UMR 5046, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 38054 Grenoble Cedex 9, France
    Photochem Photobiol Sci 1:565-9. 2002
    ..In both cases, their yield of formation was found to be, at least, 2 orders of magnitude lower than the overall yield of bipyrimidine photoproducts such as cyclobutane dimers and (6-4) photoproducts...
  20. ncbi Oxidatively generated complex DNA damage: Tandem and clustered lesions
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB UMR E no 3 CEA UJF, Institut Nanosciences et Cryogenie, CEA Grenoble, F 38054 Grenoble Cedex 9, France Département de Médecine Nucléaire et Radiobiologie, Faculté de médecine de des sciences de la santé, Universite de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4 Electronic address
    Cancer Lett 327:5-15. 2012
    ..These consist mainly of double strand breaks (DSBs) and non-DSB clustered lesions that are referred as to oxidatively generated clustered DNA lesions (OCDLs)...
  21. ncbi Cross-linked thymine-purine base tandem lesions: synthesis, characterization, and measurement in gamma-irradiated isolated DNA
    Sophie Bellon
    , Service de Chimie Inorganique et Biologique, UMR 5046, , CEA-Grenoble, F-38054 Grenoble Cedex 9, France
    Chem Res Toxicol 15:598-606. 2002
    ..Moreover, a significant sequence effect was observed. Thus, the yield of formation of the tandem lesions is higher when the purine base is located at the 5' position of the 5-(2'-deoxyuridilyl)methyl radical...
  22. ncbi Larger yield of cyclobutane dimers than 8-oxo-7,8-dihydroguanine in the DNA of UVA-irradiated human skin cells
    Sophie Courdavault
    , Service de Chimie Inorganique et Biologique, , CEA-Grenoble, 17, avenue des Martyrs, 38054 Grenoble Cedex 9, France
    Mutat Res 556:135-42. 2004
    ..The predominant formation of CPDs with respect to oxidative damage within normal human skin cells exposed to UVA radiation should be taken into account in photoprotection strategies...
  23. ncbi Mechanism of nitric oxide induced deamination of cytosine
    Vanessa Labet
    Laboratoire des Lésions des Acides Nucléiques, INAC SCIB UMR E no 3 CEA UJF, CEA Grenoble, F 38054, Grenoble, France
    Phys Chem Chem Phys 11:2379-86. 2009
    ..Thus, the two products of the reaction consist of a nitrogen molecule and the enol tautomer of uracil in equilibrium with the keto form...
  24. ncbi Repair of oxidative damage of thymine by HeLa whole-cell extracts: simultaneous analysis using a microsupport and comparison with traditional PAGE analysis
    Valérie Guerniou
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 17, rue des Martyrs, 38054 Grenoble Cedex 9, France
    Biochimie 87:151-9. 2005
    ..Moreover, extended study of 5-carboxyuracil revealed that this lesion displays similar biological properties as 5-formyluracil...
  25. ncbi Oxidatively generated damage to the guanine moiety of DNA: mechanistic aspects and formation in cells
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB UMR E n3 CEA UJF Institut Nanosciences et Cryogénie, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Acc Chem Res 41:1075-83. 2008
    ..We discuss these chemical reactions in the context of *OH radical, singlet oxygen, and two-quantum photoionization processes...
  26. ncbi Oxidatively generated damage to DNA by UVA radiation in cells and human skin
    Jean Cadet
    Laboratoire Lésions de Acides Nucléiques, SCIB UMR E no 3, CEA UJF, FRE CNRS 3200, Departement de Recherche Fondamentale sur la Matiere Condensee, Grenoble, France
    J Invest Dermatol 131:1005-7. 2011
    ..The mechanisms of UVA-mediated formation of DNA oxidation products indicate the absence of induction of double-strand breaks...
  27. ncbi 5',8-Cyclo-2'-deoxyadenosine (cdA) formation by gamma-radiation. Theoretical quantum mechanics study
    Boleslaw T Karwowski
    Laboratoire des Lésions des Acides Nucléiques, INAC SCIB UMR E n degrees 3 CEA UJF, CEA Grenoble, Grenoble Cedex 9, France
    Acta Biochim Pol 56:655-62. 2009
    ..The O4'-exo conformation was found to be present in each of the discussed mechanisms...
  28. ncbi Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB UMR E no 3 CEA UJF, Institut Nanosciences et Cryogénie CEA Grenoble, 38054 Grenoble, France
    Photochem Photobiol Sci 8:903-11. 2009
    ..This leads to the predominant formation of 8-oxo-7,8-dihydroguanine together with smaller amounts of oxidized pyrimidine bases and DNA strand breaks in UVA-irradiated cells...
  29. ncbi Repair of the three main types of bipyrimidine DNA photoproducts in human keratinocytes exposed to UVB and UVA radiations
    Sophie Courdavault
    , Service de Chimie Inorganique et Biologique, , CEA-Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
    DNA Repair (Amst) 4:836-44. 2005
    ..Altogether, our results show that CPDs are the most frequent and the less efficiently repaired bipyrimidine photoproducts irrespectively of the applied UV treatment...
  30. ncbi Biologically relevant oxidants and terminology, classification and nomenclature of oxidatively generated damage to nucleobases and 2-deoxyribose in nucleic acids
    Jean Cadet
    Direction des Sciences de Matière, Institut Nanosciences et Cryogenie, CEA Grenoble, Grenoble Cedex 9, France
    Free Radic Res 46:367-81. 2012
    ....
  31. ncbi Hydrolytic deamination of 5-methylcytosine in protic medium--a theoretical study
    Vanessa Labet
    Laboratoire Lesions des Acides Nucleiques, INAC SCIB UMR E no 3 CEA UJF, CEA Grenoble, 17 rue des Martyrs, F 38 054 Grenoble Cedex 9, France
    J Phys Chem A 113:2524-33. 2009
    ..Both electrostatic and electron-transfer contributions appear to have significant importance. In vacuum, the former one dominates when the substrate is positively charged and the latter one when it is neutral...
  32. ncbi Chemical synthesis and biochemical properties of oligonucleotides that contain the (5'S,5S,6S)-5',6-cyclo-5-hydroxy-5,6-dihydro-2'-deoxyuridine DNA lesion
    Evelyne Muller
    , Service de Chimie Inorganique et Biologique, UMR 5046, , 38054 Grenoble Cedex 9, France
    Chembiochem 3:534-42. 2002
    ..Therefore, (5'S,5S,6S)-cyclo-5-OH-dHdU may represent a potential lethal lesion within the cell if it is not removed by the nucleotide excision repair machinery...
  33. ncbi One-electron oxidation of the guanine moiety of 2'-deoxyguanosine: influence of 8-oxo-7,8-dihydro-2'-deoxyguanosine
    Jean Luc Ravanat
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, UMR 5046, DRFMC, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    J Am Chem Soc 125:2030-1. 2003
    ..It could be concluded that 8-oxodGuo efficiently protects dGuo from decomposition by the one-electron oxidation reaction...
  34. ncbi Oxidatively generated base damage to cellular DNA
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB UMR E CEA UJF Institut Nanosciences et Cryogénie, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Free Radic Biol Med 49:9-21. 2010
    ..Information on nucleobase modifications that are formed upon addition of reactive aldehydes arising from the breakdown of lipid hydroperoxides is also provided...
  35. ncbi Oxidation of the sugar moiety of DNA by ionizing radiation or bleomycin could induce the formation of a cluster DNA lesion
    Peggy Regulus
    Laboratoire des Lésions des Acides Nucléiques, Departement de Recherche Fondamentale sur la Matiere Condensee, Commissariat a l Energie Atomique Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
    Proc Natl Acad Sci U S A 104:14032-7. 2007
    ..The 2'-deoxycytidine adducts thus characterized that represent the first example of complex DNA lesions isolated and identified in cellular DNA upon one radical hit are likely to play an important role in the toxicity of bleomycin...
  36. ncbi Radical oxidation of the adenine moiety of nucleoside and DNA: 2-hydroxy-2'-deoxyadenosine is a minor decomposition product
    Sandrine Frelon
    , Service de Chimie Inorganique et Biologique, UMR 5046, , CEA/Grenoble, France
    Free Radic Res 36:499-508. 2002
    ....
  37. ncbi Recent aspects of oxidative DNA damage: guanine lesions, measurement and substrate specificity of DNA repair glycosylases
    Jean Cadet
    Service de Chimie Inorganique et Biologique and UMR 5046, , CEA/Grenoble, France
    Biol Chem 383:933-43. 2002
    ..Finally, recently available data on the substrate specificity of DNA repair enzymes belonging to the base excision pathways are briefly reviewed...
  38. ncbi Comet assay coupled to repair enzymes for the detection of oxidative damage to DNA induced by low doses of gamma-radiation: use of YOYO-1, low-background slides, and optimized electrophoresis conditions
    Sylvie Sauvaigo
    , , Service de Chimie Inorganique et Biologique-CNRS-UMR 5046, CEA Grenoble, F-38054 Grenoble Cedex 9, France
    Anal Biochem 303:107-9. 2002
  39. ncbi Recognition of cyclonucleoside lesions by the Lactococcus lactis FPG protein
    Evelyne Muller
    , Service de Chimie Inorganique et Biologique and UMR 5046, DRFMC-CEA/Grenoble, Grenoble, France
    Nucleosides Nucleotides Nucleic Acids 22:1563-5. 2003
    ..This may provide new information on the mechanism involved in the activity of the latter enzyme...
  40. ncbi Formation of the spore photoproduct and other dimeric lesions between adjacent pyrimidines in UVC-irradiated dry DNA
    Thierry Douki
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, FRE 2600, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 38054 Grenoble 9, France
    Photochem Photobiol Sci 2:433-6. 2003
    ..Using this approach, the complete distribution of bipyrimidine lesions within UVC-irradiated dry DNA was determined...
  41. ncbi Mechanistic aspects of the oxidation of DNA constituents mediated by singlet molecular oxygen
    Jean-Luc Ravanat
    , FRE 2600, , CEA Grenoble, France
    Arch Biochem Biophys 423:23-30. 2004
    ..As a striking observation, the secondary oxidation reaction of 8-oxodGuo, a main 1O2 oxidation product of dGuo, may explain, at least partly, the observed mutagenicity of 1O2...
  42. ncbi Excision of 8-methylguanine site-specifically incorporated into oligonucleotide substrates by the AlkA protein of Escherichia coli
    Didier Gasparutto
    , Service de Chimie Inorganique et Biologique, UMR 5046 CEA-CNRS-UJF, , CEA-Grenoble, 17 Avenue des Martyrs F-38054 Cedex 9, Grenoble, France
    DNA Repair (Amst) 1:437-47. 2002
    ....
  43. ncbi Synthesis of a convenient thymidine glycol phosphoramidite monomer and its site-specific incorporation into DNA fragments
    Didier Gasparutto
    Labratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique, UMR E3 CEA UJF, DRFMC, CEA Grenoble, France
    Nucleosides Nucleotides Nucleic Acids 24:1831-42. 2005
    ..Then the modified nucleoside was site-specifically inserted into DNA fragments by solid support assembling followed by a "one-step" mild final deprotection treatment...
  44. ncbi Inter-strand photoproducts are produced in high yield within A-DNA exposed to UVC radiation
    Thierry Douki
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, FRE 2600, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
    Nucleic Acids Res 31:3134-42. 2003
    ....
  45. ncbi Assessment of the photoprotection properties of sunscreens by chromatographic measurement of DNA damage in skin explants
    Stéphane Mouret
    CEA, INAC, SCIB, UJF and CNRS, LCIB UMR_E 3 CEA UJF and FRE 3200, Laboratoire Lesions des Acides Nucleiques, Grenoble Cedex, France
    Photochem Photobiol 87:109-16. 2011
    ..These data show the usefulness of the proposed approach for the evaluation of the genoprotection afforded by sunscreens...
  46. ncbi Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cells
    Olivier Falletti
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, UMR E3 CEA UJF, Departement de Recherche Fondamentale sur la Matiere Condensee, Grenoble, France
    Free Radic Biol Med 42:1258-69. 2007
    ..These results emphasize the major role played by glutathione in the protection of DNA against electrophilic species...
  47. ncbi Effect of (5'S)-5',8-cyclo-2'-deoxyadenosine on the conformation of di and trinucleotides. A NMR and DFT study
    Boleslaw T Karwowski
    Laboratoire des Lésions des Acides Nucléiques, INAC SCIB UMR E n degrees 3 CEA UJF, Condensée, CEA Grenoble, 17 Avenue des Martyrs, F 38054, Grenoble Cedex 9, France
    Org Biomol Chem 6:3408-13. 2008
    ....
  48. ncbi Deamination of the radical cation of the base moiety of 2'-deoxycytidine: a theoretical study
    Vanessa Labet
    Laboratoire Lesions des Acides Nucleiques, INAC SCIB, UMR E 3 CEA UJF, CEA Grenoble, 17 Avenue des Martyrs, F 38054 Grenoble Cedex 9, France
    Chemphyschem 9:1195-203. 2008
    ..and formation of a protonated uracil. The rate-determining step of this mechanism is hydrogen-atom transfer from a water molecule to the cytosine derivative. The associated free energy barrier is 70.2 kJ mol(-1)...
  49. ncbi Measurement of oxidatively generated base damage in cellular DNA
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB UMR E n 3 CEA UJF, FRE CNRS 3200, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 711:3-12. 2011
    ..Another difficult topic is the measurement of oxidatively generated clustered DNA lesions that is currently achieved using enzymatic approaches and that would necessitate further investigations...
  50. ncbi Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers
    Isabelle Hininger
    L B S O, Universite Joseph Fourier, Grenoble, France
    Mutat Res 558:75-80. 2004
    ..01). Altogether, this study strongly suggests that frozen whole blood could be utilised in association with the comet assay in human epidemiological bio-monitoring for the assessment of genetic damage in populations at risk...
  51. ncbi [Harmful effects of solar UVA radiation: new indices in DNA]
    Thierry Douki
    , DRFMC/SCIB UMR-E 3 CEA-UJF, CEA-Grenoble, 38054 Grenoble Cedex 9, France
    Med Sci (Paris) 23:124-6. 2007
  52. ncbi Detection of new radiation-induced DNA lesions by liquid chromatography coupled to tandem mass spectrometry
    Peggy Regulus
    , DRFMC/SCIB CEA Grenoble, 17 Avenue des Martyrs, F-38054 Grenoble Cedex 9, France
    Rapid Commun Mass Spectrom 18:2223-8. 2004
    ..In addition, the latter lesion was also shown to be present in untreated cells, indicating that the modified nucleoside could be formed endogenously...
  53. ncbi Photoinduced damage to cellular DNA: direct and photosensitized reactions
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB UMR E n 3, CEA UJF, Institut Nanosciences et Cryogenie, CEA Grenoble, Grenoble Cedex, France
    Photochem Photobiol 88:1048-65. 2012
    ..Evidence has been also provided for the occurrence of photosensitized reactions mediated by exogenous agents that act either in an independent way or through photodynamic effects...
  54. ncbi Metallothionein expression in HaCaT and C6 cell lines exposed to cadmium
    Yves Nzengue
    INAC SCIB UMR E3 CEA UJF, Laboratoire Lesions des Acides Nucleiques, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    J Trace Elem Med Biol 23:314-23. 2009
    ..Our results suggested that the C6 cell sensitivity was correlated with the decrease in MT level at 20micromol/L Cd occurring after the transcription of MT gene...
  55. ncbi DNA tandem lesions containing 8-oxo-7,8-dihydroguanine and formamido residues arise from intramolecular addition of thymine peroxyl radical to guanine
    Thierry Douki
    DRFMC, Service de Chimie Inorganique et Biologique, Laboratoire des Lésions des Acides Nucléiques, CEA Grenoble, 17, avenue des Martyrs, F 38054 Grenoble Cedex 9, France
    Chem Res Toxicol 15:445-54. 2002
    ..This received support from (18)O labeling experiments...
  56. ncbi Formation of a methide derivative upon photolysis of thymidine bromohydrins
    Thierry Douki
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, UMR 5046, CEA DSM Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, France
    J Org Chem 68:478-82. 2003
    ..The proposed structure for the primary photolysis product of thymidine bromohydrins is a methide derivative of the thymine ring. This compound could be an interesting intermediate in the synthesis of methyl-substituted thymidine...
  57. ncbi Characterization of lysine-guanine cross-links upon one-electron oxidation of a guanine-containing oligonucleotide in the presence of a trilysine peptide
    Sandrine Perrier
    , DRFMC/SCIB UMR-E3 CEA-UJF, CEA-Grenoble, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France
    J Am Chem Soc 128:5703-10. 2006
    ..KEYWORDS: oxidatively generated DNA damage, photosensitization, guanine radical cation, DNA-protein cross-links...
  58. ncbi Radiation-induced formation of DNA intrastrand crosslinks between thymine and adenine bases: A theoretical approach
    Bertrand Xerri
    , , CEA/Grenoble, 17 Avenue des Martyrs, 38054, Grenoble Cedex 9, France
    Org Biomol Chem 4:3986-92. 2006
    ....
  59. ncbi Singlet oxygen-mediated damage to cellular DNA determined by the comet assay associated with DNA repair enzymes
    Jean-Luc Ravanat
    , FRE 2600, DRFMC/SCIB CEA Grenoble, 17 Avenue des Martyrs, F-38054 Grenoble Cedex 9, France
    Biol Chem 385:17-20. 2004
    ..In contrast, significant amounts of direct strand breaks and alkali-labile sites or oxidised pyrimidines, detectable by the bacterial endonuclease III, were not produced...
  60. ncbi Nuclear magnetic resonance studies of the 4R and 4S diastereomers of spiroiminodihydantoin 2'-deoxyribonucleosides: absolute configuration and conformational features
    Boleslaw Karwowski
    Laboratoire de Chimie Inorganique et Biologique, UMR-E No. 3 (CEA-UJF, , CEA/Grenoble, F-38054 Grenoble Cedex 9, France
    Chem Res Toxicol 19:1357-65. 2006
    ..In addition, information is provided on the conformational features of the 2-deoxyribose moiety and the orientation of the base around the N-glycosidic bond of both 2'-deoxyribonucleosides 1 and 2...
  61. ncbi Hydroxyl radical is not the main reactive species involved in the degradation of DNA bases by copper in the presence of hydrogen peroxide
    Sandrine Frelon
    , UMR 5046, , CEA-Grenoble, 38054 Grenoble Cedex 9, France
    Chem Res Toxicol 16:191-7. 2003
    ..In contrast, the formation of the sole 8-oxo-7,8-dihydroguanine upon incubation of DNA with Cu(II) ions and H(2)O(2) strongly suggests the production of singlet oxygen as the predominant reactive oxygen species...
  62. ncbi Identification of the alpha and beta anomers of 1-(2-deoxy-D-erythro-pentofuranosyl)-oxaluric acid at the site of riboflavin-mediated photooxidation of guanine in 2'-deoxyguanosine and thymidylyl-(3'-5')-2'-deoxyguanosine
    Garry W Buchko
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA
    Photochem Photobiol 82:191-9. 2006
    ....
  63. ncbi [18O]-labeled singlet oxygen as a tool for mechanistic studies of 8-oxo-7,8-dihydroguanine oxidative damage: detection of spiroiminodihydantoin, imidazolone and oxazolone derivatives
    Glaucia R Martinez
    , , , Brazil
    Biol Chem 383:607-17. 2002
    ....
  64. ncbi Development and application of a novel immunoassay for measuring oxidative DNA damage in the environment
    David L Mitchell
    Department of Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville 78957, USA
    Photochem Photobiol 75:257-65. 2002
    ..These data demonstrate the usefulness of these assays for environmental photobiology and the potential for their use in studying the relative impacts of UVB versus UVA, including ozone depletion events...
  65. ncbi Is 5-aminolevulinic acid involved in the hepatocellular carcinogenesis of acute intermittent porphyria?
    Janice Onuki
    Departamento de Bioquimica, Instituto de Quimica, USP, Sao Paulo, Brasil
    Cell Mol Biol (Noisy-le-grand) 48:17-26. 2002
    ..Together these results reinforce the hypothesis that the DNA damage induced by ALA may be associated with the development of HCC in individuals suffering from AIP...
  66. ncbi Are we sure we know how to measure 8-oxo-7,8-dihydroguanine in DNA from human cells?
    Andrew R Collins
    Institute for Nutrition Research, University of Oslo, Norway
    Arch Biochem Biophys 423:57-65. 2004
    ..The background level of DNA oxidation in normal human cells is likely to be around 0.3-4.2 8-oxoGua per 10(6) Gua. An effort should be made to develop alternative, validated methods for estimating oxidative DNA damage...
  67. ncbi Formation of isodialuric acid lesion within DNA oligomers via one-electron oxidation of 5-hydroxyuracil: characterization, stability and excision repair
    Philippe Simon
    , , UMR E3 CEA-UJF, CEA-Grenoble, F-38054 Grenoble Cedex 9, France
    Nucleic Acids Res 34:3660-9. 2006
    ..Moreover, biochemical features dealing with the substrate specificity of several bacterial and yeast base excision repair enzymes to remove isodialuric acid from site-specifically modified DNA fragments were determined...
  68. ncbi The histone octamer is invisible when NF-kappaB binds to the nucleosome
    Dimitar Angelov
    , INSERM U309, Institut Albert Bonniot, Domaine de la Merci, 38706 La Tronche Cedex, France
    J Biol Chem 279:42374-82. 2004
    ..This peculiar property of NF-kappaB p50 might have evolved to meet the requirements for its function as a central switch for stress responses...
  69. ncbi DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis
    Ralf Moeller
    German Aerospace Center DLR, Radiation Biology Division, Institute of Aerospace Medicine, Linder Hoehe, 51147 Cologne, Germany
    Arch Microbiol 188:421-31. 2007
    ..In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation...
  70. ncbi ATP-dependent chromatin remodeling is required for base excision repair in conventional but not in variant H2A.Bbd nucleosomes
    Hervé Menoni
    Laboratoire Joliot Curie, CNRS USR3010, Ecole Normale Superieure de Lyon, 69364 Lyon Cedex 7, France
    Mol Cell Biol 27:5949-56. 2007
    ..A very weak effect of SWI/SNF on the 8-oxoG BER removal in H2A.Bbd histone variant nucleosomes was observed. The possible implications of our data for the understanding of in vivo mechanisms of BER are discussed...
  71. ncbi One-electron oxidation of DNA and inflammation processes
    Jean Cadet
    Nat Chem Biol 2:348-9. 2006
  72. ncbi Spiroiminodihydantoin nucleoside formation from 2'-deoxyguanosine oxidation by [(18)O-labeled] singlet molecular oxygen in aqueous solution
    Glaucia Regina Martinez
    Departamento de Bioquimica e Biologia Molecular, Setor de Ciencias Biologicas, Universidade Federal do Parana, Curitiba, Brazil
    J Mass Spectrom 42:1326-32. 2007
    ....
  73. ncbi Selective one-electron oxidation of duplex DNA oligomers: reaction at thymines
    Avik Ghosh
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Org Biomol Chem 6:916-28. 2008
    ..In the latter case, addition may initiate a tandem reaction that converts both thymines of a TT step to oxidation products...
  74. ncbi Oxidation of 2'-deoxycytidine to four interconverting diastereomers of N1-carbamoyl-4,5-dihydroxy-2-oxoimidazolidine nucleosides
    Sebastien Tremblay
    Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Science, University of Sherbrooke, Quebec, Canada J1H 5N4
    J Org Chem 72:3672-8. 2007
    ..The rate of isomerization was greater for cis-diastereomers compared to trans-diastereomers, and the rate sharply increased with pH (pH 9.0>7.0>5.5)...
  75. ncbi Detection of chlorinated DNA and RNA nucleosides by HPLC coupled to tandem mass spectrometry as potential biomarkers of inflammation
    Carine Badouard
    , DRFMC/SCIB CEA Grenoble, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France
    J Chromatogr B Analyt Technol Biomed Life Sci 827:26-31. 2005
    ..The method was found to be sensitive enough to allow the measurement of background levels of 5-chloro-2'-deoxycytidine in the DNA of human white blood cells isolated from 7 mL of blood...
  76. ncbi Identification of the main oxidation products of 8-methoxy-2'-deoxyguanosine by singlet molecular oxygen
    Glaucia R Martinez
    , , , , Brazil
    Free Radic Biol Med 38:1491-500. 2005
    ..These results provided new mechanistic insights into the reactions of O2 (1Delta(g)) with purine nucleosides...
  77. ncbi UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination
    Ralf Moeller
    German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany
    Int Microbiol 10:39-46. 2007
    ..SP-lyase-deficient spores repaired 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination...
  78. ncbi Riboflavin and UV-light based pathogen reduction: extent and consequence of DNA damage at the molecular level
    Vijay Kumar
    Navigant Biotechnologies, 1215 Quail Street, Lakewood, CO 80215, USA
    Photochem Photobiol 80:15-21. 2004
    ..These data also suggest that the type and extent of damage to DNA for virus in the presence of RB and light make it less likely to be repaired by normal repair pathways available in host cells...
  79. ncbi Cell surface expression of melanocortin-1 receptor on HaCaT keratinocytes and alpha-melanocortin stimulation do not affect the formation and repair of UVB-induced DNA photoproducts
    Geneviève Garcin
    UMR CNRS 5247 IBMM, Université Montpellier I Faculté de Pharmacie, 15 Av Charles Flahault BP 14 491, 34093, Montpellier Cedex 5, and Department of Dermatology, Groupe Hospitalo Universitaire Caremeau, Nimes, France
    Photochem Photobiol Sci 6:585-93. 2007
    ..In conclusion, cell surface-enforced expression of MC1Rs on HaCaT keratinocytes and alpha-MSH stimulation do not affect the formation of UVB-induced DNA photoproducts and their subsequent repair...