Research Topics
| J CadetSummaryCountry: France Publications
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Publications
Oxidative base damage to DNA: specificity of base excision repair enzymesJ Cadet
Departement de Recherche Fondamentale sur la Matiere Condensee, SCIB Laboratoire Lésions des Acides Nucléiques, CEA Grenoble, 17 Avenue des Martyrs, Grenoble, France
Mutat Res 462:121-8. 2000..As one of the main conclusions, it appears that the specificity of both endo III and Fpg proteins is much broader than expected a few years ago...- Hydroxyl radicals and DNA base damageJ Cadet
Departement de Recherche Fondamentale sur la Matiere Condensee, SCIB Laboratoire Lésions des Acides Nucléiques, CEA Grenoble, F 38054, Grenoble Cedex 9, France
Mutat Res 424:9-21. 1999..Therefore, major efforts should be devoted to the reassessment of the level of oxidative base damage in cellular DNA using appropriate assays including suitable conditions of DNA extraction... - Facts and artifacts in the measurement of oxidative base damage to DNAJ Cadet
Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, France
Free Radic Res 29:541-50. 1998..Further efforts should be made to resolve this apparent discrepancy. In addition, the question of the biological validation of the non-invasive measurement of oxidized bases and nucleosides in urine is addressed... - Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins: kinetic and mechanistic aspectsC D'Ham
Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, France
Biochemistry 38:3335-44. 1999.... - Simultaneous determination of five oxidative DNA lesions in human urineJ L Ravanat
Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, 17 Avenue des Martyrs, F 38054 Grenoble Cedex 9, France
Chem Res Toxicol 12:802-8. 1999..Attempts to determine the amount of 5,6-dihydroxy-5,6-dihydrothymine, 5-hydroxycytosine, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine using the above HPLC-GC/MS method were unsuccessful... 
Determination of new types of DNA lesions in human spermC Badouard
Laboratoire des Lésions des Acide Nucléiques LCIB UMR E 3 CEA UJF DRFMC, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
Zygote 16:9-13. 2008..All of this decay has to be repaired by the oocytes at the time of fertilization or immediately after. This aspect should not be overlooked in assisted reproductive technology, in order to understand risks and limitations...- Oxidatively generated DNA lesions as potential biomarkers of in vivo oxidative stressJ L Ravanat
Laboratoire Lesions des Acides Nucleiques, SCIB UMR E 3 CEA UJF, Institut Nanosciences et Cryogenie, CEA Grenoble, 17 rue des Martyrs, F38054 Grenoble cedex 9, France
Curr Mol Med 12:655-71. 2012..In addition, recent work highlighting the possible formation of complex DNA lesions will be reported and commented regarding the possibility of using such complex damage as potential biomarkers of oxidative stress... - Analysis of fluoroquinolone-mediated photosensitization of 2'-deoxyguanosine, calf thymus and cellular DNA: determination of type-I, type-II and triplet-triplet energy transfer mechanism contributionS Sauvaigo
, , Grenoble, France
Photochem Photobiol 73:230-7. 2001..Therefore, we propose that the phototoxic effects of FQ are mostly accounted for energy transfer mechanism rather than by Type-I or -II photosensitization processes... - The role of duck hepatitis B virus and aflatoxin B1 in the induction of oxidative stress in the liverL Barraud
INSERM U271, Grenoble, France
Cancer Detect Prev 25:192-201. 2001.... - Resveratrol enhances UVA-induced DNA damage in HaCaT human keratinocytesM Seve
Laboratoire des Lésions des Acides Nucléiques, DRFMC SCIB, CEA Grenoble, Grenoble, France
Med Chem 1:629-33. 2005..The conclusion is a potential hazardous effect of topical application of resveratrol, particularly on regions exposed to sunlight... - Epoxide adducts at the guanine residue within single-stranded DNA chains: reactivity and stability studiesD Gasparutto
Laboratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique UMR CEA UJF, DRFMC CEA Grenoble, Grenoble Cedex 9, France
Nucleosides Nucleotides Nucleic Acids 24:545-52. 2005.... - MALDI-TOF mass spectrometry as a powerful tool to study enzymatic processing of DNA lesions inserted into oligonucleotidesD Gasparutto
Laboratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique UMR 5046, DRFMC, CEA Grenoble, Grenoble Cedex, France
Nucleosides Nucleotides Nucleic Acids 22:1583-6. 2003..The latter analytical approach was shown to be an informative and efficient alternative technique to conventional electrophoresis and chromatographic analyses... - Direct and indirect effects of UV radiation on DNA and its componentsJ L Ravanat
, Service de Chimie Inorganique et Biologique, UMR 5046, , CEA Grenoble, 17 Avenue des Martyrs, F-38054 Grenoble Cedex 9, France
J Photochem Photobiol B 63:88-102. 2001..In addition, it was found that UVA photooxidation damage mostly involves the guanine residues of cellular DNA as the result of singlet oxygen generation by still unknown endogenous photosensitizers... - Gas chromatographic-mass spectrometric determination of 5-hydroxymethyluracil in human urine by stable isotope dilutionH Faure
Laboratoire de Biochimie C, C H U, Grenoble, France
J Chromatogr 616:1-7. 1993..Between-run precision of this method was 7.79%, and concentrations as low as 1.87 nM could be measured. This sensitivity and precision could not be obtained with trimethylsilyl derivatives... - Urinary excretion of 5-(hydroxymethyl) uracil in healthy volunteers: effect of active and passive tobacco smokeF Bianchini
Departement de Recherche Fondamentale sur la Matiere Condensee, SCIB LAN CEA, Grenoble, France
Int J Cancer 77:40-6. 1998..These findings suggest that measurement of urinary excretion of 5-HMUra could be useful as a biomarker of oxidative DNA damage and repair, though further research is needed to support these data... - *H atom and *OH radical reactions with 5-methylcytosineA Grand
Laboratoire Lesions des Acides Nucleiques, DRFMC SCIB, UMR E 3 CEA UJF, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
J Phys Chem A 111:8968-72. 2007..The proton hyperfine coupling constants (HFCC), computed at the IEFPCM/MPW1B95/6-311++G(2d,2p) level, agree well with B3LYP results and available experimental and theoretical data on related thymine and cytosine radicals... - 5-(phenylthiomethyl)-2'-deoxyuridine as an efficient photoreactive precursor to generate single and multiple lesions within DNA fragmentsS Bellon
, UMR 5046, , CEA-Grenoble F-38054 Grenoble Cedex 9, France
Nucleosides Nucleotides Nucleic Acids 20:967-71. 2001..The photoproducts have been isolated and further characterized on the basis of NMR and mass spectrometric analyses... - UV laser photolysis of DNA: effect of duplex stability on charge-transfer efficiencyT Douki
, 17, rue des Martyrs, F-38054 Grenoble Cedex 9, France
J Am Chem Soc 123:11360-6. 2001..This was rationalized in terms of an increase in the overall charge-transfer efficiency. Therefore, it may be concluded that stabilization of the double-helix favors charge-transfer process toward guanine bases... - Individual determination of the yield of the main UV-induced dimeric pyrimidine photoproducts in DNA suggests a high mutagenicity of CC photolesionsT Douki
, CEA/Grenoble, 17, avenue des Martyrs, F-38054 Grenoble Cedex 9, France
Biochemistry 40:2495-501. 2001..A major striking observation deals with the low yield of cytosine-cytosine photoproducts which are likely to be associated with the UV-specific CC to TT tandem mutation... - Excision by the human methylpurine DNA N-glycosylase of cyanuric acid, a stable and mutagenic oxidation product of 8-oxo-7,8-dihydroguanineC Dherin
CEA, , , BP6, F92265 Fontenay aux Roses, France
Int J Radiat Biol 80:21-7. 2004..Most of them, Sp, Gh, Oa and Oz, are substrates of the oxidized bases-specific enzymes such as Nth or Fpg. In contrast, Ca is substrate of the human methylpurine DNA N-glycosylase (Mpg)... - 2'-deoxyguanosine oxidation is associated with decrease in the DNA-binding activity of the transcription factor Sp1 in liver and kidney from diabetic and insulin-resistant ratsO Ramon
, , La Tronche, France
Free Radic Biol Med 30:107-18. 2001..Therefore, the modification in the binding efficiency of Sp1 or NF-kappaB could be related to reactive oxygen species-mediated DNA damage... - Sensitivity to polychromatic UV-radiation of strains of Deinococcus radiodurans differing in their DNA repair capacityU Pogoda de la Vega
DLR, Institute of Aerospace Medicine, Radiation Biology Division, Koeln, Germany
Int J Radiat Biol 81:601-11. 2005..The investigated strains differ in their radiation susceptibility, leading to a classification into a UV-sensitive (UVS78 and 1R1A) and a UV-resistant class (wild type strain R1 and 262)... - Chlorination of guanosine and other nucleosides by hypochlorous acid and myeloperoxidase of activated human neutrophils. Catalysis by nicotine and trimethylamineM Masuda
Unit of Endogenous Cancer Risk Factors, International Agency for Research on Cancer, 150 cours Albert Thomas, F 69372 Lyon Cedex 8, France
J Biol Chem 276:40486-96. 2001.... - New synthesis of 5-carboxy-2'-deoxyuridine and its incorporation into synthetic oligonucleotidesV Guerniou
, Service de Chimie Inorganique et Biologique, UMR 5046, , Grenoble Cedex, France
Nucleosides Nucleotides Nucleic Acids 22:1073-5. 2003..The phosphoramidite derivative has been incorporated at defined sites into oligonucleotides using the solid phase synthesis approach... 
Structural study of DNA duplex containing an N-(2-deoxy-beta-D-erythro-pentofuranosyl) formamide frameshift by NMR and restrained molecular dynamicsC Maufrais
CEA, , , Bat 144, CEA-Saclay, 91191 Gif-sur-Yvette Cedex, France
Nucleic Acids Res 31:5930-40. 2003..Information extracted from 31P NMR data reveal a modification of the phosphodiester backbone conformation at the extrahelical site, which is also observed during the molecular dynamics simulations...- Formation of modified DNA bases in cells exposed either to gamma radiation or to high-LET particlesJ-P Pouget
, , CEA/Grenoble, F-38054 Grenoble Cedex 9, France
Radiat Res 157:589-95. 2002..These studies show that the HPLC-MS/MS assay provides an accurate, reliable and sensitive method for measuring cellular DNA base damage... - Repair of the main UV-induced thymine dimeric lesions within Arabidopsis thaliana DNA: evidence for the major involvement of photoreactivation pathwaysA L Dany
, , CEA/Cadarache, 13108 Saint-Paul-lez-Durance, France
J Photochem Photobiol B 65:127-35. 2001..The removal of (6-4) adducts was found to occur more efficiently. These results strongly suggest that repair of UV-induced photolesions in plants is mainly mediated by photolyases... - Oxygen free radical damage to DNA. Translesion synthesis by human DNA polymerase eta and resistance to exonuclease action at cyclopurine deoxynucleoside residuesI Kuraoka
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
J Biol Chem 276:49283-8. 2001..These data suggest that the S diastereoisomer may be a more cytotoxic DNA lesion than the R diastereoisomer...