Location: Montpellier, France
- Two related subpellicular cytoskeleton-associated proteins in Trypanosoma brucei stabilize microtubulesCécile Vedrenne
Laboratoire de Parasitologie Moleculaire, université Victor Segalen de Bordeaux II, Unité Mixte Recherche 5016 Centre National de la Recherche Scientifique, 33076 Bordeaux, France
Mol Biol Cell 13:1058-70. 2002..When overexpressed in T. brucei, both CAP15 and CAP17 cover the whole subpellicular corset and induce morphological disorders, cell cycle-based abnormalities, and subsequent asymmetric cytokinesis...
- The AP3 adaptor is involved in the transport of membrane proteins to acidocalcisomes of LeishmaniaSebastien Besteiro
Wellcome Centre for Molecular Parasitology and Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, 120 University Place, Glasgow, G12 8TA, UK
J Cell Sci 121:561-70. 2008..Our findings, implicating that AP3 has a role in transport to acidocalcisomes, thus provide further evidence that biogenesis of acidocalcisomes resembles that of lysosome-related organelles, and that both may have conserved origins...
- Lipidomic analysis of Toxoplasma gondii tachyzoites rhoptries: further insights into the role of cholesterolSebastien Besteiro
Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques DIMNP, Universités de Montpellier II et I, Centre National de la Recherche Scientifique CNRS, UMR 5235, Montpellier, F 34095, France
Biochem J 415:87-96. 2008..Biol. Cell 14, 3804-3820], might be explained by the need of a cholesterol-rich region of the host cell we could visualize at the point of contact with the attached parasite, in conditions where parasite motility was blocked...
- Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasionSebastien Besteiro
UMR 5235 CNRS, Université de Montpellier 2, Montpelier, France
PLoS Pathog 5:e1000309. 2009..Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes...
- Overexpression of the natural inhibitor of cysteine peptidases in Leishmania mexicana leads to reduced virulence and a Th1 responseKaren Bryson
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
Infect Immun 77:2971-8. 2009..This study confirms that L. mexicana CPs are virulence factors and that ICPs have therapeutic potential...
- Inhibitor of cysteine peptidase does not influence the development of Leishmania mexicana in Lutzomyia longipalpisLucie Jecna
Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
J Med Entomol 46:605-9. 2009..mexicana ICP did not inhibit peptidase activity of the midgut extracts in vitro. We conclude that ICP has no major role in promoting survival of L. mexicana in the vectorial part of its life cycle in L. longipalpis...
- Exploring metabolomic approaches to analyse phospholipid biosynthetic pathways in PlasmodiumS Besteiro
Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235, Centre National de la Recherche Scientifique UM1 UM2, Universite Montpellier 2, cc 107, Place Eugene Bataillon, 34095 Montpellier Cedex 05, France
Parasitology 137:1343-56. 2010..Elucidating the regulation of the biosynthesis of these crucial metabolites could help design of future anti-malarial drugs...
- The RON2-AMA1 interaction is a critical step in moving junction-dependent invasion by apicomplexan parasitesMauld Lamarque
UMR 5235 CNRS, Université de Montpellier 2, Montpellier, France
PLoS Pathog 7:e1001276. 2011..gondii and P. falciparum entry into their host cells. This work provides the first evidence that AMA1 uses the rhoptry neck protein RON2 as a receptor to promote invasion by Apicomplexa parasites...
- The moving junction of apicomplexan parasites: a key structure for invasionSebastien Besteiro
UMR 5235 CNRS, DIMNP, Université de Montpellier 2, cc 107, 34095 Montpellier Cedex 05, France
Cell Microbiol 13:797-805. 2011..Ongoing and future research will certainly help refining the model by characterizing the molecular organization within the MJ and its interactions with both host and parasite cytoskeleton for anchoring of the complex...
- Protein turnover and differentiation in LeishmaniaSebastien Besteiro
Wellcome Centre for Molecular Parasitology and Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK
Int J Parasitol 37:1063-75. 2007..This review focuses on these peptidases and their role in the cellular differentiation of Leishmania through their key involvement in a variety of degradative pathways in the lysosomal and autophagy networks...
- Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalisJane M Carlton
Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
Science 315:207-12. 2007..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
- Succinate secreted by Trypanosoma brucei is produced by a novel and unique glycosomal enzyme, NADH-dependent fumarate reductaseSebastien Besteiro
Laboratoire de Parasitologie Moleculaire, UMR 5016 CNRS, Université Victor Segalen Bordeaux II, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France
J Biol Chem 277:38001-12. 2002..FRDg may have the characteristics of such an ancestral enzyme and is the only NADH-dependent fumarate reductase characterized to date...
- ATP generation in the Trypanosoma brucei procyclic form: cytosolic substrate level is essential, but not oxidative phosphorylationVirginie Coustou
Laboratoire de Genomique Fonctionnelle des Trypanosomatides, UMR 5162 CNRS, France
J Biol Chem 278:49625-35. 2003..We conclude that PYK is essential because of its ATP production, which implies that the cytosolic substrate level phosphorylation is essential for the growth of procyclic trypanosomes...
- A potential role for ICP, a Leishmanial inhibitor of cysteine peptidases, in the interaction between host and parasiteSebastien Besteiro
Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, Glasgow G11 6NU, UK
Mol Microbiol 54:1224-36. 2004....
- A mitochondrial NADH-dependent fumarate reductase involved in the production of succinate excreted by procyclic Trypanosoma bruceiVirginie Coustou
Laboratoire de Genomique Fonctionnelle des Trypanosomatides, UMR 5162 CNRS, Universite Victor Segalen Bordeaux 2, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France
J Biol Chem 280:16559-70. 2005..We propose that FRDm1 is involved in the maintenance of the redox balance in the mitochondrion, as proposed for the ancestral soluble FRD presumably present in primitive anaerobic cells...
- Energy generation in insect stages of Trypanosoma brucei: metabolism in fluxSebastien Besteiro
Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, Glasgow G11 6NU, Scotland, UK
Trends Parasitol 21:185-91. 2005..In this article, we summarize how these recent data have helped to change the view of metabolism in procyclic-form T. brucei...
- Endosome sorting and autophagy are essential for differentiation and virulence of Leishmania majorSebastien Besteiro
Wellcome Centre for Molecular Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, United Kingdom
J Biol Chem 281:11384-96. 2006..This finding, that transformation to the infective metacyclic form is dependent on late endosome function and, more directly, autophagy, makes L. major a good model for studying the roles of these processes in differentiation...
- Fumarate is an essential intermediary metabolite produced by the procyclic Trypanosoma bruceiVirginie Coustou
Laboratoire de Genomique Fonctionnelle des Trypanosomatides, UMR 5162 CNRS and Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS, Universite Victor Segalen Bordeaux 2, 146 rue Leo Saignat, 33076 Bordeaux, France
J Biol Chem 281:26832-46. 2006..Inaoka, D. K., Osanai, A., Nara, T., Odaka, M., Aoki, T., Inaka, K., Harada, S., and Kita, K. (2002) Mol. Biochem. Parasitol. 122, 189-200)...
- The SNARE protein family of Leishmania majorSebastien Besteiro
Wellcome Centre for Molecular Parasitology and Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8TA, UK
BMC Genomics 7:250. 2006..The recent availability of the genome sequence of L. major has allowed us to assess the complement of SNAREs in the parasite and to investigate their location in comparison with metazoans...
- Role of ATG3 in the parasite Toxoplasma gondii: autophagy in an early branching eukaryoteSebastien Besteiro
DIMNP, UMR 5235 CNRS, Université de Montpellier 2, Montpellier, France
Autophagy 8:435-7. 2012..gondii seemed thus able to perform macroautophagy, but the cellular functions of the pathway for this parasite remained to be demonstrated...
- Which roles for autophagy in Toxoplasma gondii and related apicomplexan parasites?Sebastien Besteiro
DIMNP, UMR5235 CNRS, Universités de Montpellier 1 and 2, Montpellier 34095, France
Mol Biochem Parasitol 184:1-8. 2012..The possible roles for autophagy during the life cycles of other medically important apicomplexan parasites and the perspectives for discovering new drug targets in this pathway for combating these parasites are discussed in this review...
- Autophagy protein Atg3 is essential for maintaining mitochondrial integrity and for normal intracellular development of Toxoplasma gondii tachyzoitesSebastien Besteiro
UMR 5235 CNRS, Universités de Montpellier 2 et 1, Dynamique des Interactions Membranaires Normales et Pathologiques, Montpellier, France
PLoS Pathog 7:e1002416. 2011..Overall, our results show that TgAtg3-dependent autophagy might be regulating mitochondrial homeostasis during cell division and is essential for the normal development of T. gondii tachyzoites...