Alain Berthod

Summary

Country: France

Publications

  1. ncbi request reprint Temperature and enantioseparation by macrocyclic glycopeptide chiral stationary phases
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon 1, CNRS 5180, 69622 Villeurbanne, France
    J Chromatogr A 1060:205-14. 2004
  2. ncbi request reprint Chiral recognition mechanisms
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, France
    Anal Chem 78:2093-9. 2006
  3. doi request reprint Advantages of a small-volume counter-current chromatography column
    Alain Berthod
    CNRS, Universite de Lyon, Villeurbanne, France
    J Chromatogr A 1216:4169-75. 2009
  4. ncbi request reprint Alkane effect in the Arizona liquid systems used in countercurrent chromatography
    A Berthod
    Laboratoire des Sciences Analytiques, Universite Claude Bernard, Lyon 1, 69622, Villeurbanne, France
    Anal Bioanal Chem 383:327-40. 2005
  5. ncbi request reprint Using the liquid nature of the stationary phase in countercurrent chromatography. IV. The cocurrent CCC method
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite Claude Bernard Lyon 1, Bat CPE, 60622 Villeurbanne, France
    J Chromatogr A 1116:143-8. 2006
  6. ncbi request reprint Band broadening inside the chromatographic column: the interest of a liquid stationary phase
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS 5180, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1126:347-56. 2006
  7. pmc Elution-extrusion countercurrent chromatography: theory and concepts in metabolic analysis
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, Bat CPE, 69622 Villeurbanne, France
    Anal Chem 79:3371-82. 2007
  8. ncbi request reprint Could linear solvation energy relationships give insights into chiral recognition mechanisms? 1. Pi-pi and charge interaction in the reversed versus the normal phase mode
    Alain Berthod
    Laboratoire des Sciences Analytiques, CNRS, Universite de Lyon, 69622 Villeurbanne Cedex, France
    J Chromatogr A 1166:61-9. 2007
  9. ncbi request reprint Ionic liquids in separation techniques
    A Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1184:6-18. 2008
  10. doi request reprint Chiral recognition mechanisms with macrocyclic glycopeptide selectors
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS, Bat CPE, Villeurbanne, France
    Chirality 21:167-75. 2009

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Temperature and enantioseparation by macrocyclic glycopeptide chiral stationary phases
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon 1, CNRS 5180, 69622 Villeurbanne, France
    J Chromatogr A 1060:205-14. 2004
    ....
  2. ncbi request reprint Chiral recognition mechanisms
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, France
    Anal Chem 78:2093-9. 2006
  3. doi request reprint Advantages of a small-volume counter-current chromatography column
    Alain Berthod
    CNRS, Universite de Lyon, Villeurbanne, France
    J Chromatogr A 1216:4169-75. 2009
    ..The two older CCC columns cannot compete with the new one. However, the small 19mL column with 1.6mm bore tubing can be useful when fast results are desired without top resolution...
  4. ncbi request reprint Alkane effect in the Arizona liquid systems used in countercurrent chromatography
    A Berthod
    Laboratoire des Sciences Analytiques, Universite Claude Bernard, Lyon 1, 69622, Villeurbanne, France
    Anal Bioanal Chem 383:327-40. 2005
    ....
  5. ncbi request reprint Using the liquid nature of the stationary phase in countercurrent chromatography. IV. The cocurrent CCC method
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite Claude Bernard Lyon 1, Bat CPE, 60622 Villeurbanne, France
    J Chromatogr A 1116:143-8. 2006
    ..Continuous detection is a problem due to the fact that two immiscible liquid phases elute from the column. It was partly solved using an evaporative light scattering detector...
  6. ncbi request reprint Band broadening inside the chromatographic column: the interest of a liquid stationary phase
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS 5180, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1126:347-56. 2006
    ..The cocurrent CCC method is continuous and was able to resolve cholesterol at baseline in 40 min using 110 mL. Detection is difficult due to the fact that two immiscible liquid phases enter the detector...
  7. pmc Elution-extrusion countercurrent chromatography: theory and concepts in metabolic analysis
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, Bat CPE, 69622 Villeurbanne, France
    Anal Chem 79:3371-82. 2007
    ..Its improved capabilities of KD targeting make EECCC a promising tool for the specific analysis of "small" molecules in complex samples such as in metabolomic fingerprinting and footprinting...
  8. ncbi request reprint Could linear solvation energy relationships give insights into chiral recognition mechanisms? 1. Pi-pi and charge interaction in the reversed versus the normal phase mode
    Alain Berthod
    Laboratoire des Sciences Analytiques, CNRS, Universite de Lyon, 69622 Villeurbanne Cedex, France
    J Chromatogr A 1166:61-9. 2007
    ..The TAG CSP produced logically very different parameters when used in the reversed phase mode compared to the normal phase mode showing the critical role of the mobile phase...
  9. ncbi request reprint Ionic liquids in separation techniques
    A Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1184:6-18. 2008
    ..These methods are also considered. ILs will soon be produced on an industrial scale and it will be necessary to develop reliable analytical procedures for their analysis and control...
  10. doi request reprint Chiral recognition mechanisms with macrocyclic glycopeptide selectors
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS, Bat CPE, Villeurbanne, France
    Chirality 21:167-75. 2009
    ..The remarkable complementary enantioselectivity effect observed with the four CSPs is discussed...
  11. doi request reprint Countercurrent chromatography: people and applications
    A Berthod
    Universite de Lyon, CNRS UMR, Villeurbanne, France
    J Chromatogr A 1216:4206-17. 2009
    ..Other applications are found in the pharmaceutical and chemical field. The separation of enantiomers on the preparative scale is a field of growing importance...
  12. ncbi request reprint Use of the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate in countercurrent chromatography
    A Berthod
    Laboratoire des Sciences Analytiques, CNRS, Universite de Lyon 1, Bat CPE 308, 69622 Villeurbanne, France
    Anal Bioanal Chem 380:168-77. 2004
    ....
  13. doi request reprint Distribution ratio, distribution constant and partition coefficient. Countercurrent chromatography retention of benzoic acid
    Alain Berthod
    Laboratoire des Sciences Analytiques, Universite de Lyon 1, CNRS, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1218:6024-30. 2011
    ..e. at very low concentration and pH 2. It is possible to quench dimerization adding butanol in the heptane/water system. However, butanol additions also affect the partition coefficient of benzoic acid greatly by increasing it...
  14. ncbi request reprint Evaluation of molecule-microbe interactions with capillary electrophoresis: procedures, utility and restrictions
    Alain Berthod
    Department of Chemistry, Iowa State University, Ames, IA 50011 3111, USA
    Electrophoresis 23:847-57. 2002
    ..Finally, CE can sometimes be used to determine the size of binding constants (K(RL)) within certain limits provided experimental conditions can be formulated that minimize problems of biological heterogeneity...
  15. ncbi request reprint Test to evaluate countercurrent chromatographs. Liquid stationary phase retention and chromatographic resolution
    A Berthod
    Laboratoire des Sciences Analytiques, CNRS, Universit de Lyon 1, Villeurbanne, France
    J Chromatogr A 902:323-35. 2000
    ..The internal diameter of the tubing used is shown to be a critical parameter acting on the machine volume and number of tubing turns...
  16. ncbi request reprint Super/subcritical fluid chromatography chiral separations with macrocyclic glycopeptide stationary phases
    Ying Liu
    Iowa State University, Department of Chemistry, Gilman Hall, Ames, IA 50011, USA
    J Chromatogr A 978:185-204. 2002
    ..The speed of the separations is the main advantage of the use of SFC compared to normal-phase HPLC. In addition, SFC is advantageous for preparative separations with easy solute recovery and solvent disposal...
  17. ncbi request reprint Solvent systems for countercurrent chromatography: an aqueous two phase liquid system based on a room temperature ionic liquid
    Maria Jose Ruiz-Angel
    Laboratoire des Sciences Analytiques, Universite de Lyon, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1151:65-73. 2007
    ..The discrimination factor of the ionic liquid system and its intrinsic hydrophobicity were respectively found three times higher and ten times lower than the respective values of the PEG 1000 ATPS...
  18. doi request reprint Purification of Coomassie Brilliant Blue G-250 by multiple dual mode countercurrent chromatography
    Nazim Mekaoui
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1232:134-41. 2012
    ..The purified CBB gave total satisfaction in testing amine content in polyclonal antibody containing monolith pipettes...
  19. doi request reprint Zirconia based monoliths used in hydrophilic-interaction chromatography for original selectivity of xanthines
    Jérôme Randon
    Laboratoire des Sciences Analytiques, Universite de Lyon, Universite Claude Bernard Lyon1, CNRS, Batiment Curien, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    J Chromatogr A 1217:1496-500. 2010
    ..The three isomers were easily separated in HILIC mode on a zirconia based stationary phase...
  20. ncbi request reprint Determination of liquid-liquid partition coefficients by separation methods
    A Berthod
    Laboratoire des Sciences Analytiques, CNRS, Universite de Lyon 1, Bat CPE 308, 43 Boulevard du 11 November 1918, 69622 Villeurbanne Cedex, France
    J Chromatogr A 1037:3-14. 2004
    ..Examples of applications of the knowledge of liquid-liquid partition coefficient in the vast world of solvent extraction and hydrophobicity estimation are presented...
  21. doi request reprint Thermodynamic studies of a zwitterionic stationary phase in hydrophilic interaction liquid chromatography
    Haixiao Qiu
    Department of Chemistry, University of Texas at Arlington, Arlington, TX 76019, USA
    J Chromatogr A 1272:81-9. 2013
    ....
  22. doi request reprint Advances in countercurrent chromatography for protein separations
    Nazim Mekaoui
    Laboratoire des Sciences nalytiques, Universite de Lyon, ISA CNRS, Bat CPE, 69622 Villeurbanne, France
    Bioanalysis 4:833-44. 2012
    ..Most published protein purifications were performed in academic laboratories. The highest throughput listed is 1.65 g/h of pure lyzozyme, obtained using a 5.5 l hydrostatic CCC column...
  23. ncbi request reprint Reversed-phase liquid chromatography analysis of alkyl-imidazolium ionic liquids II. Effects of different added salts and stationary phase influence
    Maria Jose Ruiz-Angel
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1189:476-82. 2008
    ..LODs in the low microgram range ( approximately 5 nmol) were obtained with the Kromasil C(8) column with a 50/50 acetonitrile-water mobile phase containing 0.01 M NaPF(6) added salt and 230 nm UV detection...
  24. doi request reprint A second-generation ionic liquid matrix-assisted laser desorption/ionization matrix for effective mass spectrometric analysis of biodegradable polymers
    Alain Berthod
    Universite de Lyon, CNRS, Laboratoire des Sciences Analytiques, 69622 Villeurbanne, France
    Rapid Commun Mass Spectrom 23:3409-22. 2009
    ....
  25. ncbi request reprint Separation of chiral sulfoxides by liquid chromatography using macrocyclic glycopeptide chiral stationary phases
    Alain Berthod
    Iowa State University, Department of Chemistry, Ames 50011, USA
    J Chromatogr A 955:53-69. 2002
    ..The macrocyclic glycopeptide CSPs provided broad selectivity and effective separations of chiral sulfoxides...
  26. ncbi request reprint Theory and use of the pseudophase model in gas-liquid chromatographic enantiomeric separations
    Veronica Pino
    Chemistry Department, Iowa State University, 1605 Gilman Hall, Ames, Iowa 50011, USA
    Anal Chem 78:113-9. 2006
    ..The three-phase model may be applied to any gas-liquid chromatography stationary phase involving a pseudophase...
  27. ncbi request reprint Uptake and enantioselective elimination of chlordane compounds by common carp (Cyprinus carpio, L.)
    Rachadaporn Seemamahannop
    Department of Chemistry, Schrenk Hall, University of Missouri Rolla, Rolla, MO 65401, USA
    Chemosphere 59:493-500. 2005
    ..7, while no enantiomeric changes were observed for the cis-chlordane. It seems that the (-)-trans-chlordane is metabolized significantly faster (t(1/2-)=15 days) in the river carp fish than the (+)-trans-enantiomer (t(1/2+)=20 days)...
  28. doi request reprint Using the liquid nature of the stationary phase. VI. Theoretical study of multi-dual mode countercurrent chromatography
    Nazim Mekaoui
    Laboratoire des Sciences Analytiques, Universite de Lyon 1, CNRS, Bat CPE, 69622 Villeurbanne, France
    J Chromatogr A 1218:6061-71. 2011
    ..The model is validated calculating correctly the peak position of previously published MDM experiments...
  29. ncbi request reprint Reversed phase liquid chromatography of alkyl-imidazolium ionic liquids
    Maria Jose Ruiz-Angel
    Laboratoire des Sciences Analytiques, Universite de Lyon, CNRS 5180, 69622 Villeurbanne, France
    J Chromatogr A 1113:101-8. 2006
    ..Also, the IL retention factor depends on the concentration and nature of the added salt. Hexafluorophosphate chaotropic anions can adsorb on the Kromasil C18 surface dramatically increasing the imidazolium cation retention factors...
  30. ncbi request reprint Bonded ionic liquid polymeric material for solid-phase microextraction GC analysis
    Eranda Wanigasekara
    Department of Chemistry, University of Texas at Arlington, Box 19065, Arlington, TX, 76019, USA
    Anal Bioanal Chem 396:511-24. 2010
    ..Their efficacy compares favorably to that of commercial fibers for polar analytes. The mechanical strength and durability of the polymeric IL fibers were excellent...
  31. ncbi request reprint Solvent properties of the 1-butyl-3-methylimidazolium hexafluorophosphate ionic liquid
    S Carda-Broch
    Department of Chemistry, Iowa State University, Ames 50011, USA
    Anal Bioanal Chem 375:191-9. 2003
    ..The positive form of amino acids is complexed by the crown ether and the complex is extracted in the ionic liquid phase...
  32. doi request reprint Use of limonene in countercurrent chromatography: a green alkane substitute
    Karine Faure
    Institut des Sciences Analytiques, CNRS, Universite de Lyon, Villeurbanne, France
    Anal Chem 85:4644-50. 2013
    ..Limonene has the capability to be a "green" alternative to petroleum-based solvents in CCC applications. ..
  33. ncbi request reprint Ionic matrices for matrix-assisted laser desorption/ionization time-of-flight detection of DNA oligomers
    Samuel Carda-Broch
    Iowa State University, Department of Chemistry, Ames, IA 50011, USA
    Rapid Commun Mass Spectrom 17:553-60. 2003
    ..In one case, an ionic matrix provided a signal-to-noise ratio ten times higher than the corresponding molecular matrix. Several of the tested ionic matrices were liquids. However, all working ionic matrices were solids...
  34. ncbi request reprint Ionic liquids versus triethylamine as mobile phase additives in the analysis of beta-blockers
    M J Ruiz-Angel
    Laboratoire des Sciences Analytiques, Universite Claude Bernard Lyon 1, Villeurbanne, France
    J Chromatogr A 1119:202-8. 2006
    ....
  35. ncbi request reprint pH dependence of the hydrophobicity of beta-blocker amine compounds measured by counter-current chromatography
    S Carda-Broch
    Laboratoire des Sciences Analytiques, CNRS, Universite de Lyon 1, Bat CPE 308, 69622 Villeurbanne, France
    J Chromatogr A 995:55-66. 2003
    ..Relationships between biological properties and hydrophobicity were also examined...
  36. ncbi request reprint Nonionic micellar liquid chromatography coupled to immobilized enzyme reactors
    S Tomer
    Department of Chemistry, University of Cincinnati, OH 45221, USA
    J Chromatogr A 923:7-16. 2001
    ..It is shown that the low efficiency obtained with micellar liquid chromatography is compensated by the possibility offered by the technique to easily adjust selectivity...
  37. ncbi request reprint Retention and selectivity of teicoplanin stationary phases after copper complexation and isotopic exchange
    A Berthod
    Laboratoire des Sciences Analytiques, CNRS, , Villeurbanne, France
    Anal Chem 73:5499-508. 2001
    ..The enantioselectivity factors of all of the molecules remain unchanged because all of the interactions are changed equally. We propose a new global quality criterion (the E factor) for comparing and evaluating enantiomeric separations...
  38. ncbi request reprint Effect of ionization and the nature of the mobile phase in quantitative structure-retention relationship studies
    M J Ruiz-Angel
    Laboratoire des Sciences Analytiques, Universite Claude Bernard Lyon 1, Villeurbanne, France
    J Chromatogr A 1063:25-34. 2005
    ..In MLC, since an electrostatic interaction is added to hydrophobic forces, poorer correlations were obtained in all cases. The retention data obtained in RPLC also seems to correlate better with the biological activity of the drugs...
  39. ncbi request reprint Evaluation of the macrocyclic glycopeptide A-40,926 as a high-performance liquid chromatographic chiral selector and comparison with teicoplanin chiral stationary phase
    A Berthod
    University of Missouri-Rolla, College of Arts and Sciences, Department of Chemistry, Rolla, MO 65401, USA
    J Chromatogr A 897:113-29. 2000
    ..It is shown that the A-40,926 CSP is complementary to the teicoplanin CSP, thereby enlarging the number of enantiomers that can be separated by the macrocyclic glycopeptide based CSPs...
  40. ncbi request reprint Chiral separation of basic drugs by capillary electrophoresis with carboxymethylcyclodextrins
    Janusz Zukowski
    GlaxoSmithKline, Analytical Chemistry, New Frontiers Science Park North, Harlow, Essex CM19 5AD, UK
    J Chromatogr A 948:331-42. 2002
    ..It is shown that the solute affinity for the chiral selector is not related to its enantioresolution factor. None of the two cyclodextrin selectors used was able to separate the whole set of basic drugs...
  41. ncbi request reprint Could linear solvation energy relationships give insights into chiral recognition mechanisms? 2. Characterization of macrocyclic glycopeptide stationary phases
    Clifford R Mitchell
    Abbott Laboratories, 1401 Sheridan Road, North Chicago, IL 60064, USA
    J Chromatogr A 1166:70-8. 2007
    ..Steric effects, seen on the v parameter, are second in magnitude followed by H-bond and polar interactions. Only one solute could be studied in the normal phase mode showing a different mechanism with polar and steric major interactions...
  42. ncbi request reprint Using the liquid nature of the stationary phase in counter-current chromatography V. The back-extrusion method
    Yanbin Lu
    Department of Chemistry, Yuquan Campus, Zhejiang University, Hangzhou 310027, Zhejiang Province, China
    J Chromatogr A 1189:10-8. 2008
    ..Otherwise, a significant portion of the solutes will stay in the mobile phase inside the column and produce a broad peak showing after the stationary phase extrusion...
  43. doi request reprint Rapid screening of bioactive components from Zingiber cassumunar using elution-extrusion counter-current chromatography
    Yanbin Lu
    Department of Chemistry, Zhejiang University, Hangzhou 310027, Zhejiang Province, China
    J Chromatogr A 1181:33-44. 2008
    ..1% pure. Compared with classical elution, the EECCC approach exhibits strong separation efficiency and great potential to be a high-throughput separation technique in the case of complex samples...
  44. ncbi request reprint Countercurrent chromatographic isolation of lolitrem B from endophyte-infected ryegrass (Lolium perenne L.) seed
    Denis Grancher
    UMR 1233 INRA ENVL, Mycotoxins and Comparative Toxicology of Xenobiotics, National School of Veterinary Medicine of Lyon, I Avenue Bourgelat, BP 83, 69280 Marcy l Etoile, France
    J Chromatogr A 1059:73-81. 2004
    ..Further purifications were then performed by thin layer and low pressure liquid chromatography. Twenty-eight micrograms of lolitrem B with a 96% purity grade were obtained from 8 kg of seeds (yield 32%)...