Research Topics
Genomes and Genes | Laurent BelecSummaryCountry: France Publications
| Collaborators
|
Detail Information
Publications
Real-time PCR-based testing of saliva for cytomegalovirus at birthLaurent Belec
Assistance Publique Hopitaux de Paris, Hopital Europeen Georges Pompidou, Laboratoire de Virologie, Paris, 15 20 rue Leblanc, 75 908 Paris Cedex 15, France
Expert Rev Anti Infect Ther 9:1119-24. 2011....- Challenges in implementing HIV laboratory monitoring in resource-constrained settings: how to do more with lessLaurent Belec
Assistance Publique, Hopitaux de Paris, Hopital Europeen Georges Pompidou, Laboratoire de Virologie, Paris, France
Future Microbiol 6:1251-60. 2011..Laboratory monitoring strategies may become more attractive as price negotiations render second-line ART regimens less expensive worldwide... - Combinatorial prevention of HIV transmission in women: the case for a vaginal microbicideLaurent Belec
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France, 15 20 rue Leblanc, 75 908 Paris Cedex 15, France
Future Microbiol 6:731-7. 2011..Finally, the combination of existing partially effective strategies for HIV prevention should be promoted, scaled-up and evaluated... 
Real-time PCR quantification of genital shedding of herpes simplex virus (HSV) and human immunodeficiency virus (HIV) in women coinfected with HSV and HIVJerome Legoff
Unité INSERM Internationale U743 Immunologie Humaine, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
J Clin Microbiol 44:423-32. 2006....- Inhibition of HIV-1 transmission in trans from dendritic cells to CD4+ T lymphocytes by natural antibodies to the CRD domain of DC-SIGN purified from breast milk and intravenous immunoglobulinsMary Requena
Unité INSERM U743, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
Immunology 123:508-18. 2008..Our data support a role of natural antibodies to DC-SIGN in the modulation of postnatal HIV transmission through breast-feeding and in the natural host defence against HIV-1 in infected individuals... 
Early archives of genetically-restricted proviral DNA in the female genital tract after heterosexual transmission of HIV-1Nicolas Chomont
Universite Paris V, Unité INSERM Internationale d Immunologie Humaine U743, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, and Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
AIDS 21:153-62. 2007....- Dynamics of enfuvirtide resistance mutations in enfuvirtide-experienced patients remaining in virological failure under salvage therapyCharlotte Charpentier
Laboratoire de Virologie, Assistance Publique Hopitaux de Paris, Hopital Europeen Georges Pompidou, Paris, France
Scand J Infect Dis 43:373-9. 2011.... - Opsonization of HIV with complement enhances infection of dendritic cells and viral transfer to CD4 T cells in a CR3 and DC-SIGN-dependent mannerHicham Bouhlal
Université René Descartes Paris V and Institut National de la Santé et de la Recherche Médicale, Unité 743, Institut des Cordeliers, Paris, France
J Immunol 178:1086-95. 2007..Thus, the virus uses complement to its advantage to facilitate early steps leading to infection following mucosal transmission of HIV... - Enterovirus RNA shedding in the genital tract of childbearing-aged women living in Central AfricaJerome Jacques
Laboratoire de Virologie, Centre Hospitalier Universitaire de Reims, France
J Med Virol 78:591-7. 2006..In conclusion, the findings demonstrated the presence of EV genomes in nearly 10% of childbearing-aged women living in Central Africa, and provided the basis of possible antenatal or perinatal transmission of EV from mother-to-child... - Independent levels of cell-free and cell-associated human immunodeficiency virus-1 in genital-tract secretions of clinically asymptomatic, treatment-naive African womenLaurent Andreoletti
Laboratoire de Virologie, Centre Hospitalier Universitaire, Reims, France
J Infect Dis 188:549-54. 2003..These features may be relevant for an understanding of HIV-1 transmission in heterosexual individuals... - Differential modulation of human lactoferrin activity against both R5 and X4-HIV-1 adsorption on epithelial cells and dendritic cells by natural antibodiesHela Saidi
Universite Rene Descartes Paris V, Unité Institut National de la Recherche et de la Santé INSERM Internationale 743 d Immunologie Humaine, Centres de Recherches Biomédicales des Cordeliers, Paris, France
J Immunol 177:5540-9. 2006..This study highlights the use of Lf derivates as microbicide candidates to limit such interferences... - Virological response and resistance profiles after 24 months of first-line antiretroviral treatment in adults living in Bangui, Central African RepublicHelene Pere
Assistance Publique Hopitaux de Paris, Hopital Europeen Georges Pompidou, Laboratoire de Virologie, France
AIDS Res Hum Retroviruses 28:315-23. 2012.... - Peptide insertions in reverse transcriptase pol gene of human immunodeficiency virus type 1 as a rare cause of persistent antiretroviral therapeutic failureVeronique Schneider
Laboratoire de Virologie, Hopital Tenon, Paris, France
Clin Microbiol Infect 10:127-36. 2004.... - Infection of macrophages and dendritic cells with primary R5-tropic human immunodeficiency virus type 1 inhibited by natural polyreactive anti-CCR5 antibodies purified from cervicovaginal secretionsJobin Eslahpazir
Université Paris V and Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 Immunologie Humaine, Centre de Recherches des Cordeliers, Paris, France
Clin Vaccine Immunol 15:872-84. 2008.... - Surveillance of antiretroviral drug resistance mutations in untreated young children living in the Central African RepublicCharlotte Charpentier
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Assistance Publique Hopitaux de Paris, Universite Paris Descartes, Paris 5, Paris, France
Antivir Ther 16:1347-50. 2011.... - HSV-2- and HIV-1- permissive cell lines co-infected by HSV-2 and HIV-1 co-replicate HSV-2 and HIV-1 without production of HSV-2/HIV-1 pseudotype particlesJerome Legoff
Universite Paris V, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 Immunologie Humaine, Centre de Recherches, Biomédicales des Cordeliers, Paris, France
Virol J 4:2. 2007..In the present study, we investigated whether HIV-1 and HSV-2 may interact at the cellular level by forming HIV-1 hybrid virions pseudotyped with HSV-2 envelope glycoproteins, as was previously reported for HSV type 1... - High prevalence of high-risk oncogenic human papillomaviruses harboring atypical distribution in women of childbearing age living in Libreville, GabonAli Si-Mohamed
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
J Med Virol 77:430-8. 2005..34; P < 0.007). The multivalent vaccine currently available against cervical carcinomas, is only active against HPV-16 and HPV-18, and will thus have a low impact in this setting... - Neutralizing monoclonal antibodies to human immunodeficiency virus type 1 do not inhibit viral transcytosis through mucosal epithelial cellsNicolas Chomont
Universite Paris V, et Unité Internationale INSERM 743 Immunologie Humaine, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
Virology 370:246-54. 2008.... - Cell-associated, non-replicating strand(+) hepatitis C virus-RNA shedding in cervicovaginal secretions from chronically HCV-infected womenLaurent Belec
Unité INSERM U430 Immunopathologie Humaine, Hopital Broussais, Université Pierre and Marie Curie Paris VI, Paris, France
J Clin Virol 27:247-51. 2003..Lack of mucosal hepatitis C virus (HCV) transmission may be due to fairly low infectivity of body fluids in HCV-infected individuals in association with yet unknown innate or acquired resistance factors in individuals exposed to the virus... - Performance of HerpeSelect and Kalon assays in detection of antibodies to herpes simplex virus type 2Jerome Legoff
Universite Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743, Immunologie Humaine, Centres de Recherches Biomédicales des Cordeliers, Hopital Europeen Georges Pompidou, Paris, France
J Clin Microbiol 46:1914-8. 2008..Low index values obtained with the HerpeSelect assay may correspond to true HSV-2 infection, in particular to nonprimary first episodes of genital HSV-2 infection, and need to be interpreted in the context of clinical history... - Potent in vitro inactivation of both free and cell-associated CCR5- and CXCR4-tropic HIV-1 by common commercial soap bars from South AfricaMohammad Ali Jenabian
Université Paris Descartes Paris V, and Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
J Acquir Immune Defic Syndr 54:340-2. 2010..These observations indicate a rapid inhibitory effect of soap and water on viral infectivity... - Foscarnet salvage therapy efficacy is associated with the presence of thymidine-associated mutations (TAMs) in HIV-infected patientsCharlotte Charpentier
Laboratoire de Virologie, AP HP, Hopital Europeen Georges Pompidou, Paris, France Université Paris Descartes, Faculte de Medecine, Paris, France
J Clin Virol 43:212-5. 2008..Salvage therapy based on foscarnet plus a thymidine analog is effective in patients with advanced-stage HIV disease and viruses harbouring multiple drug-resistance mutations... - Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins: implication for microbicide developmentHela Saidi
Unité INSERM U743, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
J Transl Med 5:28. 2007..Plant lectins are candidate for microbicide development... - Virological response and resistance profiles after 18 to 30 months of first- or second-/third-line antiretroviral treatment: a cross-sectional evaluation in HIV type 1-infected children living in the Central African RepublicCharlotte Charpentier
Assistance Publique Hopitaux de Paris, Hopital Europeen Georges Pompidou, Laboratoire de Virologie, France
AIDS Res Hum Retroviruses 28:87-94. 2012.... - Influence of storage temperature on the stability of HIV-1 RNA and HSV-2 DNA in cervicovaginal secretions collected by vaginal washingJerome Legoff
, , , and Laboratoire de Virologie, , , Paris, France
J Virol Methods 138:196-200. 2006.... - Increased polymorphism in the HR-1 gp41 env gene encoding the enfuvirtide (T-20) target in HIV-1 variants harboring multiple antiretroviral drug resistance mutations in the pol geneAli Si-Mohamed
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
J Acquir Immune Defic Syndr 44:1-5. 2007..Sequence variations in HR-1 gp41 env gene region encoding the target for T-20 have previously been reported among patients naive to inhibitory fusion... - Differential modulation of CCR5-tropic human immunodeficiency virus-1 transfer from macrophages towards T cells under interleukin-4/interleukin-13 microenvironmentHela Saidi
Unité INSERM Internationale U743 Immunologie Humaine, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
Hum Immunol 71:1-13. 2010..In conclusion, secreted cytokines within mucosae may differentially influence both the HIV-1 production within the mucosal target cells reservoir and its spread thorough the mucosal tissue... - High prevalence of respiratory viral infections in patients hospitalized in an intensive care unit for acute respiratory infections as detected by nucleic acid-based assaysJerome Legoff
Hopital Europeen Georges Pompidou, and Unité INSERM 430, Institut de Recherches biomédicales des Cordeliers, Faculté de Médecine Broussais Hôtel Dieu, Universite Pierre et Marie Curie, Paris, France
J Clin Microbiol 43:455-7. 2005..Molecular detection allows for high-rate detection of respiratory viral infections in adult patients suffering from severe pneumonia... - Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteriaCharlotte Charpentier
Laboratoire de Virologie, Assistance Publique Hopitaux de Paris AP HP, Hopital Europeen Georges Pompidou, Universite Paris Descartes, Paris 5, 20 rue Leblanc, 75015 Paris, France
Arch Virol 156:1603-6. 2011..1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity... - Distribution of HIV-1 and HSV-2 epidemics in Chad revealing HSV-2 hot-spot in regions of high-risk HIV spreadCharlotte Charpentier
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, and Faculté de Médecine Paris Descartes, Paris, France
J Infect Dev Ctries 5:64-7. 2011..We were interested in assessing the distribution of HIV-1 and HSV-2 epidemics at the national level in Chad... - Comparison of washing and swabbing procedures for collecting genital fluids to assess shedding of human immunodeficiency virus type 1 (HIV-1) RNA in asymptomatic HIV-1-infected womenLaurent Andreoletti
Laboratoire de Virologie, Centre Hospitalier Universitaire, IFR 53 EA 3309, Faculté de Médecine de Reims, Reims, France
J Clin Microbiol 41:449-52. 2003..71; P = 0.0006), indicating that cervicovaginal washing and endocervical swabbing are equivalent collection procedures... - In vitro synergistic activity against CCR5-tropic HIV-1 with combinations of potential candidate microbicide molecules HHA, KRV2110 and enfuvirtide (T20)Mohammad Ali Jenabian
Université Paris Descartes Paris V, and Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
J Antimicrob Chemother 64:1192-5. 2009..Indeed, the combination of microbicides with complementary mechanisms of action is expected to increase the antiviral potency of the formulation... - Apical interactions of HIV type 1 with polarized HEC-1 cell monolayer modulate R5-HIV type 1 spread by submucosal macrophagesHela Saidi
Université Paris Descartes Paris V, Unité INSERM U743 Immunologie Humaine, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
AIDS Res Hum Retroviruses 25:497-509. 2009..These observations challenge the question of the in vivo involvement of HIV-1 as a supraepithelial stimulus that likely modulates the susceptibility for HIV-1 of submucosal target cells in favor of its transmission... - Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonizationMohammad Ali Jenabian
Université Paris Descartes Paris V, Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
AIDS Res Ther 7:16. 2010..HIV-1 in genital secretions may be opsonized by several molecules including complement components. Opsonized HIV-1 by complement enhances the infection of various mucosal target cells, such as dendritic cells (DC) and epithelial cells... - Mucosal humoral immune response to hepatitis C virus E1/E2 surface glycoproteins and HCV shedding in saliva and cervicovaginal fluids from chronically HCV-infected patientsLaurent Belec
Unité INSERM U430 Immunopathologie Humaine, Hopital Broussais, and Université Pierre and Marie Curie Paris VI, Paris, France
J Hepatol 38:833-42. 2003.... - Early impairment of CD8+ T cells immune response against Epstein-Barr virus (EBV) antigens associated with high level of circulating mononuclear EBV DNA load in HIV infectionJerome Legoff
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris
J Clin Immunol 24:125-34. 2004..These observations likely provide a basis for appreciating the risk to develop non-Hodgkin's lymphomas in HIV-infected individuals... - R5- and X4-HIV-1 use differentially the endometrial epithelial cells HEC-1A to ensure their own spread: implication for mechanisms of sexual transmissionHela Saidi
Unité INSERM U743 Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, Cedex 06, France
Virology 358:55-68. 2007..This study suggests that R5 and X4 viruses can differentially use epithelial cells to ensure their own spread... - Performance of the BioPlex 2200 multiplexing immunoassay platform for the detection of herpes simplex virus type 2 specific antibodies in African settingsJerome Legoff
Universite Paris Diderot, Sorbonne Paris Cité, INSERM U941, and Laboratoire de Virologie, Hopital Saint Louis, APHP, Paris, France
Clin Vaccine Immunol 18:1191-3. 2011..The sensitivities and specificities were, respectively, 88.9% and 93.5% for BioPlex and 89.9% and 92.7% for HerpeSelect. The agreement between both assays was 95.7%... - Unexpected high prevalence of herpes simplex virus (HSV) type 2 seropositivity and HSV genital shedding in pregnant women living in an East Paris suburban areaJerome Legoff
Universite Paris V, Unité Inserm 743, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
Int J STD AIDS 18:593-5. 2007.... - Virological failure and HIV type 1 drug resistance profiles among patients followed-up in private sector, Douala, CameroonCharlotte Charpentier
Assistance Publique, Hopitaux de Paris, Hopital Europeen Georges Pompidou, Laboratoire de Virologie, Paris, France
AIDS Res Hum Retroviruses 27:221-30. 2011..In conclusion, our observations highlight the absolute need for improving viral load assessment in resource-limited settings to prevent and/or monitor therapeutic failure... - Opsonization of HIV-1 by semen complement enhances infection of human epithelial cellsHicham Bouhlal
, , , Paris, France
J Immunol 169:3301-6. 2002.... - Residual HIV-1 RNA and HIV-1 DNA production in the genital tract reservoir of women treated with HAART: the prospective ANRS EP24 GYNODYN studyOdile Launay
Faculté de Médecine Paris Descartes, Universite Paris Descartes, Paris, France
Antivir Ther 16:843-52. 2011..The objective of this study was to prospectively assess residual HIV-1 RNA and HIV-1 DNA production in the genital tract reservoir of women initiating HAART over an 18-month period... - Modulation of HIV Binding to Epithelial Cells and HIV Transfer from Immature Dendritic Cells to CD4 T Lymphocytes by Human Lactoferrin and its Major Exposed LF-33 PeptideLaetitia Carthagena
Inserm U743 Team Immunité et Biothérapie Muqueuse, Paris, France
Open Virol J 5:27-34. 2011.... - Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza A and influenza B viruses and respiratory syncytial viruses in childrenJerome Legoff
Laboratoire de Microbiologie, Hopital Europeen Georges Pompidou, and Universite Paris Descartes, Paris, France
J Clin Microbiol 46:789-91. 2008..In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods... - Allele frequencies and haplotypes of eight Y-short tandem repeats in Bantu population living in Central AfricaMaxime Lecerf
Unité INSERM U743 Immunologie Humaine, Centre de Recherches Biomedicales des Cordeliers, and Université René Descartes Paris V, Paris, France
Forensic Sci Int 171:212-5. 2007..5% of individuals. In the Bantu population in Central Africa, the haplotype diversity and the discrimination capacity of Y-STR may be estimated at 99.14% and 0.5333, respectively... - Sustained high proportion of zidovudine-resistant HIV variants despite prolonged substitution of zidovudine by other nucleoside reverse transcriptase inhibitorsLaurent Belec
Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France
J Med Virol 68:1-6. 2002.... - [Herpes serology for genital herpes]Jerome Legoff
Universite Paris V, Laboratoire de Virologie, Hopital Europeen Georges Pompidou, F 75015 Paris, France
Presse Med 37:1251-60. 2008..The indication for type-specific serology must be discussed according to clinical context. The value of non-type-specific serology is limited... - Detection and quantitation of BK virus DNA by real-time polymerase chain reaction in the LT-ag gene in adult renal transplant recipientsAli Si-Mohamed
Laboratoire de Microbiologie, Unité de virologie, Hopital Europeen Georges Pompidou, 20 rue Leblanc, 75908 Paris Cedex 15, France
J Virol Methods 131:21-7. 2006.... - Enhanced transcytosis of R5-tropic human immunodeficiency virus across tight monolayer of polarized human endometrial cells under pro-inflammatory conditionsMarie Paule Carreno
Institut National de la Sante et de la Recherche Medicale, Unité 430, Hopital Broussais, Paris, France
Cytokine 20:289-94. 2002..Our findings suggest that transcytosis may be a relevant mechanism for the passage of virus through the genital mucosa in vivo, particularly when inflammatory cells and mediators are present in the vicinity of the mucosal surface... - Binding of LFA-1 (CD11a) to intercellular adhesion molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) triggers transmigration of human immunodeficiency virus type 1-infected monocytes through mucosal epithelial cellsMarie Paule Carreno
Unité 430, Hopital Broussais, Institut National de la Santé et de la Recherche Médicale Université Pierre et Marie Curie Paris VI, Paris, France
J Virol 76:32-40. 2002..Understanding the fundamental aspects of the initial HIV entry process during sexual transmission remains a critical step for preventing human infection and developing further vaccinal strategies and virucidal agents... - Semiquantitative detection of JCV-DNA in peripheral blood leukocytes from HIV-1-infected patients with or without progressive multifocal leukoencephalopathyLaurent Andreoletti
Institut National de la Sante et de la Recherche Medicale INSERM, Unité 430, Hopital Broussais, Paris, France
J Med Virol 66:1-7. 2002.... - Compartmentalization of HIV-1 between breast milk and blood of HIV-infected mothersPierre Becquart
Unité INSERM U430, Hopital Broussais, Paris, France
Virology 300:109-17. 2002..The observation of a compartmentalization of HIV-1 between peripheral blood and breast milk emphasizes that postnatal transmission of HIV occurs with variants that may not be predicted from the analysis of circulating viral populations...