Paulo Tavares

Summary

Affiliation: Avenue de la Terrasse
Country: France

Publications

  1. doi request reprint Automated classification of tailed bacteriophages according to their neck organization
    Anne Lopes
    CEA, iBiTecS, Gif sur Yvette, F 91191 Paris, France
    BMC Genomics 15:1027. 2014
  2. doi request reprint Genome gating in tailed bacteriophage capsids
    Paulo Tavares
    Unité de Virologie Moléculaire et Structurale, Gif sur Yvette, France
    Adv Exp Med Biol 726:585-600. 2012
  3. pmc Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance
    Paulo Tavares
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France
    Infect Immun 73:1771-8. 2005
  4. pmc The nuclease domain of the SPP1 packaging motor coordinates DNA cleavage and encapsidation
    Charlène Cornilleau
    Unité de Virologie Moléculaire et Structurale, UPR 3296 CNRS, 91190 Gif sur Yvette, France
    Nucleic Acids Res 41:340-54. 2013
  5. pmc Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating
    Sophie Lhuillier
    Unité de Virologie Moléculaire et Structurale, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 2472, Institut National de la Recherche Agronomique, 91198 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 106:8507-12. 2009
  6. doi request reprint Origin and function of the two major tail proteins of bacteriophage SPP1
    Isabelle Auzat
    Unité de Virologie Moléculaire et Structurale, CNRS UMR 2472, INRA UMR1157 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Mol Microbiol 70:557-69. 2008
  7. pmc Direct interaction of the bacteriophage SPP1 packaging ATPase with the portal protein
    Leonor Oliveira
    Unité de Virologie Moléculaire et Structurale, Unite Mixte de Recherche, CNRS 2472, Institut Fédératif de Recherche 1157, and IFR 115, Bâtiment 14B, Avenue de la Terrasse, 91190 Gif sur Yvette, France
    J Biol Chem 285:7366-73. 2010
  8. ncbi request reprint Structural rearrangements between portal protein subunits are essential for viral DNA translocation
    Ana Cuervo
    Unité de Virologie Moléculaire et Structurale, Unité Mixte de Recherche UMR CNRS 2472, UMR Institut National de la Recherche Agronomique INRA 1157 and Institut Fédératif de Recherche 115, 91198 Gif sur Yvette Cedex, France
    J Biol Chem 282:18907-13. 2007
  9. doi request reprint Headful DNA packaging: bacteriophage SPP1 as a model system
    Leonor Oliveira
    Unité de Virologie Moléculaire et Structurale, CNRS UPR3296 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Virus Res 173:247-59. 2013
  10. doi request reprint First steps of bacteriophage SPP1 entry into Bacillus subtilis
    Lina Jakutyte
    Unité de Virologie Moléculaire et Structurale, CNRS UPR3296 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Virology 422:425-34. 2012

Collaborators

  • Leonor Oliveira
  • Rut Carballido-Lopez
  • Elena V Orlova
  • Marie Agnès Petit
  • Raphael Guerois
  • Callum Smits
  • Alfred A Antson
  • Adriano O Henriques
  • Eric Jacquet
  • Bernard Gilquin
  • Eric Raspaud
  • Sabrina Marion
  • Rudi Lurz
  • Carlos São-José
  • Isabelle Auzat
  • Lina Jakutyte
  • Sophie Lhuillier
  • Anabela Isidro
  • Rimantas Daugelavicius
  • Virginija Cvirkaite-Krupovic
  • Anne Lopes
  • Anja Dröge
  • Mario A Santos
  • Charlène Cornilleau
  • Sophie Zinn-Justin
  • Catarina Baptista
  • Frank Weise
  • Asita C Stiege
  • Siew Lay Poh
  • Juan C Alonso
  • Celia Plisson
  • Ronald Melki
  • Ana Cuervo
  • Andrey A Lebedev
  • Inês Vinga
  • Ana G Camacho
  • Isabelle Petitpas
  • Marie Christine Rigothier
  • Noureddine Atmane
  • Sandrine Brasilès
  • Mathilde Gilles
  • Guillaume Dethan
  • Guillaume Letellier
  • Nathalie Lancelot
  • Joel Couprie
  • Matthieu Gallopin
  • Fatima El Khadali
  • Catherine Berrier
  • Alexei A Vagin
  • Anabela L Isidro
  • Joanne Turner
  • Margret H Krause
  • Amineh Zafarani
  • Eleanor J Dodson
  • Marie Christine Vaney
  • Marta de Frutos
  • Helen E White
  • Jean Lepault
  • Mário Almeida Santos
  • Aranzazu Gual
  • Ana Cardona
  • Hout Khun
  • Michel Huerre
  • Nancy Guillen

Detail Information

Publications29

  1. doi request reprint Automated classification of tailed bacteriophages according to their neck organization
    Anne Lopes
    CEA, iBiTecS, Gif sur Yvette, F 91191 Paris, France
    BMC Genomics 15:1027. 2014
    ..We searched for homologs of a set of proteins encoded in the structural module using a phage learning database...
  2. doi request reprint Genome gating in tailed bacteriophage capsids
    Paulo Tavares
    Unité de Virologie Moléculaire et Structurale, Gif sur Yvette, France
    Adv Exp Med Biol 726:585-600. 2012
    ..Recent exciting advances are providing new molecular insights on the mechanisms that ensure precise coordination of these critical steps required both for stable viral genome packaging and for its efficient release to initiate infection...
  3. pmc Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance
    Paulo Tavares
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France
    Infect Immun 73:1771-8. 2005
    ....
  4. pmc The nuclease domain of the SPP1 packaging motor coordinates DNA cleavage and encapsidation
    Charlène Cornilleau
    Unité de Virologie Moléculaire et Structurale, UPR 3296 CNRS, 91190 Gif sur Yvette, France
    Nucleic Acids Res 41:340-54. 2013
    ..This switch of activities of the nuclease domain is critical to achieve the viral chromosome packaging cycle...
  5. pmc Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating
    Sophie Lhuillier
    Unité de Virologie Moléculaire et Structurale, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 2472, Institut National de la Recherche Agronomique, 91198 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 106:8507-12. 2009
    ..This stopper system prevents release of packaged DNA. Disulfide cross-linking between beta-strands of the stopper blocks the key conformational changes that control genome ejection from the virus at the beginning of host infection...
  6. doi request reprint Origin and function of the two major tail proteins of bacteriophage SPP1
    Isabelle Auzat
    Unité de Virologie Moléculaire et Structurale, CNRS UMR 2472, INRA UMR1157 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Mol Microbiol 70:557-69. 2008
    ..The carboxyl-terminal extension is an elaboration present in some tailed bacteriophages. Different extensions were found to combine in a mosaic fashion with the MTP essential module in a subset of Siphoviridae genomes...
  7. pmc Direct interaction of the bacteriophage SPP1 packaging ATPase with the portal protein
    Leonor Oliveira
    Unité de Virologie Moléculaire et Structurale, Unite Mixte de Recherche, CNRS 2472, Institut Fédératif de Recherche 1157, and IFR 115, Bâtiment 14B, Avenue de la Terrasse, 91190 Gif sur Yvette, France
    J Biol Chem 285:7366-73. 2010
    ..Identification of mutations in gp6 that allow for assembly of the ATPase-portal complex but impair DNA packaging support an intricate cross-talk between the two proteins for activity of the DNA translocation motor...
  8. ncbi request reprint Structural rearrangements between portal protein subunits are essential for viral DNA translocation
    Ana Cuervo
    Unité de Virologie Moléculaire et Structurale, Unité Mixte de Recherche UMR CNRS 2472, UMR Institut National de la Recherche Agronomique INRA 1157 and Institut Fédératif de Recherche 115, 91198 Gif sur Yvette Cedex, France
    J Biol Chem 282:18907-13. 2007
    ..We propose that the alpha-5 structural rearrangements serve to coordinate ATPase activity with the positions of portal tunnel loops relative to the DNA double helix...
  9. doi request reprint Headful DNA packaging: bacteriophage SPP1 as a model system
    Leonor Oliveira
    Unité de Virologie Moléculaire et Structurale, CNRS UPR3296 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Virus Res 173:247-59. 2013
    ..Recent advances are providing new molecular insights on the mechanisms that ensure precise coordination of these critical steps required to accomplish the packaging encapsidation cycle...
  10. doi request reprint First steps of bacteriophage SPP1 entry into Bacillus subtilis
    Lina Jakutyte
    Unité de Virologie Moléculaire et Structurale, CNRS UPR3296 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Virology 422:425-34. 2012
    ..A model describing the early events of bacteriophage SPP1 infection is presented...
  11. pmc Bacteriophage infection in rod-shaped gram-positive bacteria: evidence for a preferential polar route for phage SPP1 entry in Bacillus subtilis
    Lina Jakutyte
    Laboratoire de Virologie Moleculaire et Structurale, Centre de Recherche de Gif, CNRS UPR 3296 and IFR 115, Avenue de la Terrasse, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    J Bacteriol 193:4893-903. 2011
    ..Altogether, our results reveal that the preferentially polar topology of SPP1 receptors on the surface of the host cell determines the site of phage DNA entry and subsequent replication, which occurs in discrete foci...
  12. ncbi request reprint Modulation of the viral ATPase activity by the portal protein correlates with DNA packaging efficiency
    Leonor Oliveira
    Unité de Virologie Moléculaire et Structurale, Unité Mixte de Recherche CNRS 2472, UMR INRA 1157 and Institut Fédératif de Recherche 115, Bâtiment 14B, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    J Biol Chem 281:21914-23. 2006
    ..Our results demonstrated that the portal protein has an active control over the viral ATPase activity that correlates with the performance of the DNA packaging motor...
  13. doi request reprint Oligomerization of the SPP1 scaffolding protein
    Siew Lay Poh
    Unité de Virologie Moléculaire et Structurale, UMR CNRS 2472, UMR INRA 1157 and IFR 115, Bat 14B, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    J Mol Biol 378:551-64. 2008
    ..Although native tetramers were active in assembly, refolded gp11-His(6) showed enhanced binding to gp13 revealing a more active species for interaction with the major capsid protein than native gp11-His(6)...
  14. doi request reprint A touch of glue to complete bacteriophage assembly: the tail-to-head joining protein (THJP) family
    Isabelle Auzat
    Laboratoire de Virologie Moleculaire et Structurale, Centre de Recherche de Gif, CNRS UPR 3296 and IFR115, 91198, Gif sur Yvette, France
    Mol Microbiol 91:1164-78. 2014
    ..This family of proteins can also provide fidelity to termination of the tail tube elongation reaction in a subset of phages including coliphage lambda. ..
  15. pmc A kinetic analysis of DNA ejection from tailed phages revealing the prerequisite activation energy
    Eric Raspaud
    Laboratoire de Physique des Solides, Universite Paris Sud, CNRS, UMR 8502, F 91405 Orsay cedex, France
    Biophys J 93:3999-4005. 2007
    ..The common value of the activation energy likely reflects the existence for all phages of an optimum value, ensuring a compromise between efficient DNA delivery and high stability of the virus...
  16. ncbi request reprint The minor capsid protein gp7 of bacteriophage SPP1 is required for efficient infection of Bacillus subtilis
    Inês Vinga
    Unité de Virologie Moléculaire et Structurale, CNRS UMR 2472, INRA UMR1157 and IFR 115, Bâtiment 14B, CNRS, 91198 Gif sur Yvette, France
    Mol Microbiol 61:1609-21. 2006
    ..This feature is proposed to play a central role in supporting correct routing of the phage genome from the virion to the cell cytoplasm...
  17. pmc Virus evolution toward limited dependence on nonessential functions of the host: the case of bacteriophage SPP1
    Virginija Cvirkaite-Krupovic
    Unité de Virologie Moléculaire et Structurale, CNRS UPR3296, Centre de Recherche de Gif, Gif sur Yvette, France
    J Virol 89:2875-83. 2015
    ..subtilis rarely meets conditions for exponential growth. Our results in combination with those reported for other virus-host systems suggest that bacterial viruses have evolved toward limited dependence on nonessential host functions...
  18. ncbi request reprint A defined in vitro system for DNA packaging by the bacteriophage SPP1: insights into the headful packaging mechanism
    Leonor Oliveira
    Unité de Virologie Moléculaire et Structurale, UMR CNRS 2472, UMR INRA 1157 and IFR 115, Bat 14B, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    J Mol Biol 353:529-39. 2005
    ..These findings suggest that the formation of free DNA ends that are generated by pac cleavage in vivo is the rate-limiting step in processive headful DNA packaging...
  19. ncbi request reprint Signal transduction through the Gal-GalNAc lectin of Entamoeba histolytica involves a spectrin-like protein
    Sabrina Marion
    Unité de Biologie Cellulaire du Parasitisme, Institut National de la Sante et de la Recherche Medicale U389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Mol Biochem Parasitol 135:31-8. 2004
    ..This is the first report identifying an amino acid sequence involved in the interaction between the Gal-GalNAc lectin and cytoskeletal spectrin...
  20. pmc Structural framework for DNA translocation via the viral portal protein
    Andrey A Lebedev
    York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK
    EMBO J 26:1984-94. 2007
    ....
  21. ncbi request reprint The high-resolution functional map of bacteriophage SPP1 portal protein
    Anabela Isidro
    Max Plank Institut für Molekulare Genetik, Berlin, Germany
    Mol Microbiol 51:949-62. 2004
    ..The work provides a framework for the understanding of central processes in the assembly of viruses that use specialized portals to govern entry and exit of DNA from the viral capsid...
  22. ncbi request reprint Bacillus subtilis bacteriophage SPP1 DNA packaging motor requires terminase and portal proteins
    Ana G Camacho
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Biol Chem 278:23251-9. 2003
    ..Based on these results, we propose a model in which the modulation of the ATPase and endonuclease activities of G2P accounts for the role of the terminase in headful packaging...
  23. ncbi request reprint Pressure built by DNA packing inside virions: enough to drive DNA ejection in vitro, largely insufficient for delivery into the bacterial cytoplasm
    Carlos São-José
    Instituto de Ciência Aplicada e Tecnologia and Departamento de Biologia Vegetal, Faculdade de Ciencias de Lisboa, Ed ICAT, 1749 016 Lisboa, Portugal
    J Mol Biol 374:346-55. 2007
    ....
  24. pmc Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection
    Celia Plisson
    School of Crystallography, Birkbeck College, University of London, London, UK
    EMBO J 26:3720-8. 2007
    ..This leads to opening of the connector culminating in DNA exit from the head into the host cell through the tail tube...
  25. ncbi request reprint The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA
    Carlos São-José
    Faculdade de Ciencias de Lisboa, Instituto de Ciência Aplicada e Tecnologia e Departamento de Biologia Vegetal, 1749 016 Lisboa, Portugal
    J Biol Chem 281:11464-70. 2006
    ..The fiber is predicted to be anchored in the cell membrane through transmembrane segments. These features, highly suitable for a virus receptor in Gram-positive bacteria, are very likely shared by a large number of phage receptors...
  26. ncbi request reprint The portal protein plays essential roles at different steps of the SPP1 DNA packaging process
    Anabela Isidro
    Instituto de Tecnologia Quimica e Biologica, 2781 901 Oeiras, Portugal
    Virology 322:253-63. 2004
    ..The similarity between the architecture of portal oligomers and between the DNA packaging strategies of viruses using portals strongly suggests that the portal protein plays the same roles in a large number of viruses...
  27. ncbi request reprint Specific targeting of a DNA-binding protein to the SPP1 procapsid by interaction with the portal oligomer
    Asita C Stiege
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, D 14195 Berlin, Germany
    Mol Microbiol 49:1201-12. 2003
    ....
  28. pmc Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy
    Elena V Orlova
    Imperial College of Science, Technology and Medicine, Department of Biological Sciences, Biochemistry Building, London SW7 2AY, UK
    EMBO J 22:1255-62. 2003
    ..Gp16 acts as a valve whose closure prevents DNA leakage, while its opening is required for DNA release upon interaction of the virus with its host...
  29. pmc Fate of Entamoeba histolytica during establishment of amoebic liver abscess analyzed by quantitative radioimaging and histology
    Marie Christine Rigothier
    Laboratoire de Biologie et Contrôle des Organismes Parasites, UPRES 398 IFR 75, Faculte de Pharmacie, Universite Paris Sud, France
    Infect Immun 70:3208-15. 2002
    ..Our results show that the host response promotes massive parasite death but also suggest also that this is a major contributor to the establishment of inflammation during development of liver abscess...