Marie France Carlier

Summary

Affiliation: Avenue de la Terrasse
Country: France

Publications

  1. doi request reprint Actin filament dynamics using microfluidics
    Marie France Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette, France Electronic address
    Methods Enzymol 540:3-17. 2014
  2. pmc Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility
    C Egile
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 146:1319-32. 1999
  3. ncbi request reprint GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin-related protein (ARP2/3) complex
    M F Carlier
    Dynamique du Cytosquelette, Cristallographie et RMN Biologiques, Laboratoire d Enzymologie et Biochimie Structurale, CNRS 91198 Gif sur Yvette, Paris, France Pasteur
    J Biol Chem 275:21946-52. 2000
  4. ncbi request reprint Actin-based motility as a self-organized system: mechanism and reconstitution in vitro
    Marie France Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    C R Biol 326:161-70. 2003
  5. ncbi request reprint Signalling to actin: the Cdc42-N-WASP-Arp2/3 connection
    M F Carlier
    Laboratoire d Enzymologie et Biochimie Structurale, CNRS, 91198 Gif sur Yvette, France
    Chem Biol 6:R235-40. 1999
  6. ncbi request reprint Control of actin assembly dynamics in cell motility
    Marie France Carlier
    Cytoskeleton Dynamics and Motility Group, Laboratoire d Enzymologie et Biochimie Structurale, CNRS, Gif sur Yvette 91198, France
    J Biol Chem 282:23005-9. 2007
  7. ncbi request reprint Control of actin dynamics
    M F Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Curr Opin Cell Biol 10:45-51. 1998
  8. ncbi request reprint Actin-based motility: from molecules to movement
    Marie France Carlier
    Dynamique du Cytosquelette, LEBS, CNRS, 91198 Gif sur Yvette, France
    Bioessays 25:336-45. 2003
  9. pmc Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility
    M F Carlier
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    J Cell Biol 136:1307-22. 1997
  10. ncbi request reprint Structure, function, and evolution of the beta-thymosin/WH2 (WASP-Homology2) actin-binding module
    Marie France Carlier
    Cytoskeleton Dynamics and Motility, LEBS, CNRS, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Ann N Y Acad Sci 1112:67-75. 2007

Collaborators

Detail Information

Publications72

  1. doi request reprint Actin filament dynamics using microfluidics
    Marie France Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette, France Electronic address
    Methods Enzymol 540:3-17. 2014
    ....
  2. pmc Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility
    C Egile
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 146:1319-32. 1999
    ..VASP is not involved in Shigella movement, and the function of profilin does not require its binding to proline-rich regions...
  3. ncbi request reprint GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin-related protein (ARP2/3) complex
    M F Carlier
    Dynamique du Cytosquelette, Cristallographie et RMN Biologiques, Laboratoire d Enzymologie et Biochimie Structurale, CNRS 91198 Gif sur Yvette, Paris, France Pasteur
    J Biol Chem 275:21946-52. 2000
    ..These results suggest that Grb2 may activate Arp2/3 complex-mediated actin polymerization downstream from the receptor tyrosine kinase signaling pathway...
  4. ncbi request reprint Actin-based motility as a self-organized system: mechanism and reconstitution in vitro
    Marie France Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    C R Biol 326:161-70. 2003
    ....
  5. ncbi request reprint Signalling to actin: the Cdc42-N-WASP-Arp2/3 connection
    M F Carlier
    Laboratoire d Enzymologie et Biochimie Structurale, CNRS, 91198 Gif sur Yvette, France
    Chem Biol 6:R235-40. 1999
    ....
  6. ncbi request reprint Control of actin assembly dynamics in cell motility
    Marie France Carlier
    Cytoskeleton Dynamics and Motility Group, Laboratoire d Enzymologie et Biochimie Structurale, CNRS, Gif sur Yvette 91198, France
    J Biol Chem 282:23005-9. 2007
  7. ncbi request reprint Control of actin dynamics
    M F Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Curr Opin Cell Biol 10:45-51. 1998
    ..Cellular factors that are involved in linking initiation of barbed end assembly to cell signaling are being identified using Listeria monocytogenes and Saccharomyces cerevisiae as model systems...
  8. ncbi request reprint Actin-based motility: from molecules to movement
    Marie France Carlier
    Dynamique du Cytosquelette, LEBS, CNRS, 91198 Gif sur Yvette, France
    Bioessays 25:336-45. 2003
    ....
  9. pmc Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility
    M F Carlier
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    J Cell Biol 136:1307-22. 1997
    ..In conclusion, the function of ADF is not to sequester G-actin. ADF uses ATP hydrolysis in actin assembly to enhance filament dynamics...
  10. ncbi request reprint Structure, function, and evolution of the beta-thymosin/WH2 (WASP-Homology2) actin-binding module
    Marie France Carlier
    Cytoskeleton Dynamics and Motility, LEBS, CNRS, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Ann N Y Acad Sci 1112:67-75. 2007
    ..The results provide insight into the structural basis for the regulation of the multiple functions of the WH2 domain...
  11. doi request reprint Multifunctionality of the beta-thymosin/WH2 module: G-actin sequestration, actin filament growth, nucleation, and severing
    Clotilde Husson
    Centre de Recherche de Gif, Gif sur Yvette, France
    Ann N Y Acad Sci 1194:44-52. 2010
    ..Novel regulation pathways in actin assembly emerge from these additional activities...
  12. pmc A biomimetic motility assay provides insight into the mechanism of actin-based motility
    Sebastian Wiesner
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
    J Cell Biol 160:387-98. 2003
    ..These data support models in which the rates of filament branching and capping control velocity, and autocatalytic branching of filament ends, rather than filament nucleation, occurs at the particle surface...
  13. ncbi request reprint Analysis of tetramethylrhodamine-labeled actin polymerization and interaction with actin regulatory proteins
    Andrea Pelikan Conchaudron
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurale, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Biol Chem 281:24036-47. 2006
    ..The data suggest that TMR labeling hinders the intramolecular movements of actin that allow its specific adaptative recognition by regulatory proteins and that determine its function in the ATP- or ADP-bound state...
  14. ncbi request reprint Analysis of the function of Spire in actin assembly and its synergy with formin and profilin
    Montserrat Bosch
    Cytoskeleton Dynamics and Motility Group, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    Mol Cell 28:555-68. 2007
    ..The synergy between Spire and formin is reconstituted in an in vitro motility assay, which provides a functional basis for the genetic interplay between Spire, formin, and profilin in oogenesis...
  15. ncbi request reprint Formin is a processive motor that requires profilin to accelerate actin assembly and associated ATP hydrolysis
    Stephane Romero
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Cell 119:419-29. 2004
    ..We successfully reconstitute formin-induced motility in vitro, demonstrating that this mechanism accounts for the puzzlingly rapid formin-induced actin processes observed in vivo...
  16. ncbi request reprint How ATP hydrolysis controls filament assembly from profilin-actin: implication for formin processivity
    Stephane Romero
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Biol Chem 282:8435-45. 2007
    ..Formin does not change the activity of profilin but simply uses it for its processive walk at barbed ends. Finally, if profilin release from actin is prevented by a chemical cross-link, formin processivity is abolished...
  17. ncbi request reprint Control of actin dynamics by proteins made of beta-thymosin repeats: the actobindin family
    Maud Hertzog
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Biol Chem 277:14786-92. 2002
    ..In addition, proteins of the actobindin family interact with the pointed end of actin filaments and inhibit pointed end growth, maybe via the interaction of the beta-thymosin repeats with two terminal subunits...
  18. ncbi request reprint The beta-thymosin/WH2 domain; structural basis for the switch from inhibition to promotion of actin assembly
    Maud Hertzog
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, Institut Alfred Fessard, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
    Cell 117:611-23. 2004
    ....
  19. pmc Individual actin filaments in a microfluidic flow reveal the mechanism of ATP hydrolysis and give insight into the properties of profilin
    Antoine Jégou
    Laboratoire d Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, CNRS, Gif sur Yvette, France
    PLoS Biol 9:e1001161. 2011
    ..These results emphasize the potential of microfluidics in elucidating actin regulation at the scale of individual filaments...
  20. pmc Mammalian twinfilin sequesters ADP-G-actin and caps filament barbed ends: implications in motility
    Emmanuele Helfer
    Cytoskeleton Dynamics and Motility, LEBS, CNRS, Gif sur Yvette, France
    EMBO J 25:1184-95. 2006
    ..A structural model for binding of twinfilin to barbed ends is proposed based on the similar foldings of twinfilin ADF-H domains and gelsolin segments...
  21. pmc How VASP enhances actin-based motility
    Stanislav Samarin
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Cell Biol 163:131-42. 2003
    ..VASP increases the rate of dissociation of the branch junction from immobilized ActA, which is the rate-limiting step in the catalytic cycle of site-directed filament branching...
  22. pmc How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly
    Dominique Didry
    Laboratoire d Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, CNRS, Gif sur Yvette, France
    EMBO J 31:1000-13. 2012
    ..The results open perspectives for elucidating the functions of βT/WH2 domains in other modular proteins...
  23. ncbi request reprint Coupling of folding and binding of thymosin beta4 upon interaction with monomeric actin monitored by nuclear magnetic resonance
    Michael Domanski
    Institut de Chimie des Substances Naturelles, Laboratoire de Chimie et Biologie Structurales, CNRS, 1 Avenue de la Terrasse, F 91190 Gif sur Yvette, France
    J Biol Chem 279:23637-45. 2004
    ..The combination of our NMR results and previously published mutation and cross-link data allows a better characterization of the binding mode of thymosins on G-actin...
  24. ncbi request reprint The effect of stathmin phosphorylation on microtubule assembly depends on tubulin critical concentration
    Phedra Amayed
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Biol Chem 277:22718-24. 2002
    ..We propose that stathmin acts solely by sequestering tubulin, without affecting microtubule dynamics, and that the effect of stathmin phosphorylation on microtubule assembly depends on tubulin critical concentration...
  25. pmc The IQGAP1 protein is a calmodulin-regulated barbed end capper of actin filaments: possible implications in its function in cell migration
    Andrea Pelikan-Conchaudron
    Cytoskeleton Dynamics and Motility Group, UPR 3289, Laboratoire d Enzymologie et Biochimie Structurale, CNRS, 91198 Gif sur Yvette, France
    J Biol Chem 286:35119-28. 2011
    ..The present results open perspectives for tackling the function of IQGAP1 in more complex reconstituted systems...
  26. pmc Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein
    Christophe Le Clainche
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, UPR3082, 91198 Gif sur Yvette, France
    J Biol Chem 285:23420-32. 2010
    ..Finally, we show that vinculin and the formin mDia1, which is involved in the processive elongation of actin filaments in focal adhesions, compete for actin filament barbed ends...
  27. ncbi request reprint Listeria protein ActA mimics WASp family proteins: it activates filament barbed end branching by Arp2/3 complex
    R Boujemaa-Paterski
    Dynamique du Cytosquelette, LEBS, CNRS, 91198 Gif-sur Yvette, France
    Biochemistry 40:11390-404. 2001
    ..VASP therefore affects another still unidentified biochemical reaction that plays an important role in actin-based movement...
  28. ncbi request reprint Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
    T P Loisel
    Dynamique du Cytosquelette, LEBS, CNRS, Gif sur Yvette, France
    Nature 401:613-6. 1999
    ..The movement is more effective when profilin, alpha-actinin and VASP (for Listeria) are also included. These results have implications for our understanding of the mechanism of actin-based motility in cells...
  29. doi request reprint Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation
    Julien Pernier
    Cytoskeleton Dynamics and Motility Group, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Nat Struct Mol Biol 20:1069-76. 2013
    ..Thus, VopF does not nucleate filaments by capping a pointed-end F-actin hexamer. These properties may contribute to VopF pathogenicity, and they show how dimeric WH2 peptides may mediate processive filament growth. ..
  30. pmc ATP hydrolysis on actin-related protein 2/3 complex causes debranching of dendritic actin arrays
    Christophe Le Clainche
    Dynamique du Cytosquelette et Motilité Cellulaire, Laboratoire d Enzymologie et Biochimie Structurale, Centre National de la Recherche Scientifique 1, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 100:6337-42. 2003
    ..Finally, we propose that hydrolysis of ATP on G-actin in the ternary G-actin-WASP-Arp2/3 complex on branch formation destabilizes the WASP-actin interface and energetically facilitates the detachment step in the branching reaction...
  31. ncbi request reprint IQGAP1 stimulates actin assembly through the N-WASP-Arp2/3 pathway
    Christophe Le Clainche
    Dynamics of Cytoskeleton and Motility Group, CNRS, Gif sur Yvette, France
    J Biol Chem 282:426-35. 2007
    ..We propose that signal-induced relief of the autoinhibited fold of IQGAP1 allows activation of N-WASP to stimulate Arp2/3-dependent actin assembly...
  32. pmc How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach
    Beata Bugyi
    Cytoskeleton Dynamics and Motility, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    EMBO J 29:14-26. 2010
    ..These results unveil the mechanism by which tropomyosin generates two morphologically and dynamically segregated actin networks from a single one...
  33. ncbi request reprint Role of nucleotide exchange and hydrolysis in the function of profilin in action assembly
    I Perelroizen
    Laboratoire d Enzymologie, CNRS, 91198 Gif sur Yvette, France
    J Biol Chem 271:12302-9. 1996
    ....
  34. ncbi request reprint Mechanism of actin-based motility
    D Pantaloni
    Dynamique du Cytosquelette, Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
    Science 292:1502-6. 2001
    ....
  35. pmc Arp2/3 controls the motile behavior of N-WASP-functionalized GUVs and modulates N-WASP surface distribution by mediating transient links with actin filaments
    Vincent Delatour
    Cytoskeleton Dynamics and Motility, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Biophys J 94:4890-905. 2008
    ..Computed surface distributions of N-WASP, derived from a theoretical description of this segregation-diffusion mechanism, account satisfactorily for the measured density profiles of N-WASP, Arp2/3 complex, and actin...
  36. pmc The interplay between viscoelastic and thermodynamic properties determines the birefringence of F-actin gels
    Emmanuele Helfer
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, UPR 9063 Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Biophys J 89:543-53. 2005
    ....
  37. doi request reprint Cordon-Bleu uses WH2 domains as multifunctional dynamizers of actin filament assembly
    Clotilde Husson
    Cytoskeleton Dynamics and Cell Motility, Laboratoire d Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Mol Cell 43:464-77. 2011
    ..Hence, with similar structural organization of WH2 repeats, Cordon-Bleu and Spire display different functions by selecting different sets of the multifunctional properties of WH2 domains...
  38. doi request reprint Control of actin filament treadmilling in cell motility
    Beata Bugyi
    Cytoskeleton Dynamics and Cell Motility Group, CNRS, UPR 3082, Laboratoire d Enzymologie et Biochimie Structurales, 91198 Gif sur Yvette, France
    Annu Rev Biophys 39:449-70. 2010
    ..Novel biophysical methods involving single-molecule measurements should foster progress in these crucial issues...
  39. ncbi request reprint Activation of Arp2/3 complex by Wiskott-Aldrich Syndrome protein is linked to enhanced binding of ATP to Arp2
    C Le Clainche
    Dynamique du Cytosquelette, Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
    J Biol Chem 276:46689-92. 2001
    ..WA.Arp2/3. Arp2 has a greater specificity than Arp3 for ATP versus ATP analogs. Using functional assays of actin polymerization in branched filaments, we show that binding of ATP to Arp2 is required for filament branching...
  40. pmc Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes
    V Laurent
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Cell Biol 144:1245-58. 1999
    ..We propose a molecular ratchet model for insertional polymerization of actin, within which frequent attachment-detachment of VASP to F-actin allows its sliding along the growing filament...
  41. doi request reprint Formin mDia1 senses and generates mechanical forces on actin filaments
    Antoine Jégou
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91190 Gif sur Yvette, France
    Nat Commun 4:1883. 2013
    ..Our findings suggest that formin function in cells is tightly coupled to the mechanical activity of other machineries...
  42. doi request reprint How do in vitro reconstituted actin-based motility assays provide insight into in vivo behavior?
    Beata Bugyi
    Cytoskeleton Dynamics and Motility Group, LEBS, UPR 3082, CNRS, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
    FEBS Lett 582:2086-92. 2008
    ..Differences between the cellular and biomimetic systems suggest that additional regulatory mechanisms take place in lamellipodia...
  43. ncbi request reprint Measurements of stathmin-tubulin interaction in solution
    Marie France Carlier
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette, France
    Methods Mol Med 137:103-10. 2007
    ....
  44. doi request reprint Actin-based motility assay
    Christophe Le Clainche
    Laboratoire d Enzymologie et Biochimie Structurales LEBS, Centre National de la Recherche Scientifique CNRS, Gif sur Yvette, France
    Curr Protoc Cell Biol . 2004
    ....
  45. pmc Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation
    Laure Guenin-Macé
    Institut Pasteur, Unité d Immunobiologie de l Infection, Paris, France
    J Clin Invest 123:1501-12. 2013
    ..Our findings should allow for the rationale design of competitive inhibitors of mycolactone binding to N-WASP, with anti-Buruli ulcer therapeutic potential...
  46. doi request reprint Spire and formin 2 synergize and antagonize in regulating actin assembly in meiosis by a ping-pong mechanism
    Pierre Montaville
    Laboratoire d Enzymologie et Biochimie Structurale, CNRS, Gif sur Yvette, France
    PLoS Biol 12:e1001795. 2014
    ....
  47. doi request reprint Regulation of actin assembly associated with protrusion and adhesion in cell migration
    Christophe Le Clainche
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Physiol Rev 88:489-513. 2008
    ..III) focuses on the function and the regulation of major proteins (vinculin, talin, tensin, and alpha-actinin) that control the nucleation, the binding, and the barbed-end growth of actin filaments in adhesions...
  48. doi request reprint Spire and Cordon-bleu: multifunctional regulators of actin dynamics
    Louis Renault
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette, France
    Trends Cell Biol 18:494-504. 2008
    ..In vivo, Spire and Cobl can synergize with other actin regulators. As an example, we outline potential methods to gain insight into the functional basis for reported genetic interactions among Spire, profilin and formin...
  49. pmc Force-velocity measurements of a few growing actin filaments
    Coraline Brangbour
    Laboratoire Colloïdes et Matériaux Divisés, UPMC, ESPCI ParisTech, CNRS PECSA UMR 7195, Paris, France
    PLoS Biol 9:e1000613. 2011
    ..In this mechanism, the production of force is not controlled by the polymerization rate, but is a consequence of the restriction of filaments' orientational fluctuations at their attachment point...
  50. ncbi request reprint Ciboulot regulates actin assembly during Drosophila brain metamorphosis
    I Boquet
    Laboratoire Développement, Evolution, et Plasticité du Système Nerveux, Institut de Neurobiologie Alfred Fessard, CNRS, Gif sur Yvette, France
    Cell 102:797-808. 2000
    ..Genetic experiments show that Cib and the Drosophila profilin protein Chickadee (Chic) cooperate in central brain metamorphosis...
  51. ncbi request reprint How profilin promotes actin filament assembly in the presence of thymosin beta 4
    D Pantaloni
    Laboratoire d Enzymologie Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Cell 75:1007-14. 1993
    ..When barbed ends are capped, profilin simply sequesters globular actin. A model is proposed for the function of profilin in actin-based motility...
  52. pmc Forces generated during actin-based propulsion: a direct measurement by micromanipulation
    Yann Marcy
    Laboratoire Physico Chimie Curie, Unité Mixte de Recherche 168 Institut Curie, Centre National de la Recherche Scientifique, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 5, France
    Proc Natl Acad Sci U S A 101:5992-7. 2004
    ..7 to 4.3 nN, the force-velocity relation is established. We find that the relation is linear for pulling forces and decays more weakly for pushing forces. This behavior is explained by using a dimensional elastic analysis...
  53. doi request reprint Control of actin filament dynamics at barbed ends by WH2 domains: from capping to permissive and processive assembly
    Marie France Carlier
    Cytoskeleton Dynamics and Motility Team, LEBS, CNRS, Gif sur Yvette, France
    Cytoskeleton (Hoboken) 70:540-9. 2013
    ..The details of their molecular mechanisms and the underlying structural basis provide exciting avenues in actin research...
  54. ncbi request reprint Measurement of the elasticity of the actin tail of Listeria monocytogenes
    F Gerbal
    Institut Curie UMR 168, Paris, France
    Eur Biophys J 29:134-40. 2000
    ..It provides a generic description of the various aspects of actin-tail based movements...
  55. ncbi request reprint Interaction of microtubule-associated proteins with microtubules: yeast lysyl- and valyl-tRNA synthetases and tau 218-235 synthetic peptide as model systems
    R Melki
    Laboratoire d Enzymologie, CNRS, Gif sur Yvette, France
    Biochemistry 30:11536-45. 1991
    ....
  56. pmc Hydrolysis of GTP associated with the formation of tubulin oligomers is involved in microtubule nucleation
    M F Carlier
    Laboratoire d Enzymologie et Biochimie Structurale, CNRS, Gif sur Yvetta, France
    Biophys J 73:418-27. 1997
    ..The implication of these results in a model of vectorial GTP hydrolysis in microtubule assembly is discussed...
  57. ncbi request reprint The dynamics of actin-based motility depend on surface parameters
    Anne Bernheim-Groswasser
    Laboratoire Physico Chimie Curie, UMR 168 CNRS Institut Curie, 11, rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Nature 417:308-11. 2002
    ..These results highlight how simple physical parameters such as surface geometry and protein density directly affect spatially controlled actin polymerization, and play a fundamental role in actin-dependent movement...
  58. ncbi request reprint Mutant alpha-actinin-4 promotes tumorigenicity and regulates cell motility of a human lung carcinoma
    Jeanne Menez
    Laboratoire Cytokines et Immunologie des Tumeurs Humaines, U487 INSERM, Institut Fédératif de Recherche 54, Institut Gustave Roussy, F 94805 Villejuif Cedex, France
    Oncogene 23:2630-9. 2004
    ..The identification of an inactivating mutation in ACTN4 emphasizes its role as a tumor suppressor gene and underlines the involvement of cytoskeleton alteration in tumor development and metastasis...
  59. ncbi request reprint Eps8 controls actin-based motility by capping the barbed ends of actin filaments
    Andrea Disanza
    IFOM Istituto FIRC di Oncologia Molecolare Via Adamello 16, 20139, Milan, Italy
    Nat Cell Biol 6:1180-8. 2004
    ....
  60. ncbi request reprint A novel actin barbed-end-capping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegans
    Assunta Croce
    IFOM Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20139 Milan, Italy
    Nat Cell Biol 6:1173-9. 2004
    ..Therefore, nematode genetics allowed not only the unmasking of distinct EPS-8-linked phenotypes, but also the definition of a novel function for this molecule in actin dynamics...
  61. ncbi request reprint Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes
    Metello Innocenti
    IFOM, Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20134, Milan, Italy
    Nat Cell Biol 7:969-76. 2005
    ..Thus, Abi1 is a dual regulator of WAVE and N-WASP activities in specific processes that are dependent on actin dynamics...
  62. ncbi request reprint An electrostatic steering mechanism of Cdc42 recognition by Wiskott-Aldrich syndrome proteins
    Lars Hemsath
    Abteilung Strukturelle Biologie, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Mol Cell 20:313-24. 2005
    ..This process is a prerequisite for WASp activation and a critical step in temporal regulation and integration of WASp-mediated cellular responses...
  63. ncbi request reprint Abi1 is essential for the formation and activation of a WAVE2 signalling complex
    Metello Innocenti
    IFOM Istituto FIRC di Oncologia Molecolare Via Adamello 16, 20134, Milan, Italy
    Nat Cell Biol 6:319-27. 2004
    ..Consistently, inhibition of Abi1 by RNA interference (RNAi) abrogates Rac-dependent lamellipodia protrusion. Thus, Abi1 orchestrates the proper assembly of the WAVE2 complex and mediates its activation at the leading edge in vivo...
  64. ncbi request reprint Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain
    Nalini Ramarao
    Unite de Pathogenie Microbienne Moleculaire, INSERM U786, Institut Pasteur, 75724 Paris Cedex 15, France
    FEBS Lett 581:853-7. 2007
    ..These results suggest that IpaA regulates actin polymerisation/depolymerisation at sites of Shigella invasion by modulating the barbed end capping activity of vinculin...
  65. doi request reprint Functional characterization of proteins regulating actin assembly
    Maud Hertzog
    Istituto FIRC di Oncologia Molecolare Fondazione Italiana per la Ricerca sul Cancro, Milano, Italy
    Curr Protoc Cell Biol . 2005
    ....
  66. pmc Adapter protein SH2-Bbeta stimulates actin-based motility of Listeria monocytogenes in a vasodilator-stimulated phosphoprotein (VASP)-dependent fashion
    Maria Diakonova
    Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Infect Immun 75:3581-93. 2007
    ....
  67. doi request reprint The pleckstrin homology domain of the Arf6-specific exchange factor EFA6 localizes to the plasma membrane by interacting with phosphatidylinositol 4,5-bisphosphate and F-actin
    Eric Macia
    Institut de Pharmacologie Moleculaire et Cellulaire, UMR 6097 CNRS Université de Nice Sophia Antipolis, 660, route des Lucioles, 06560 Valbonne, France
    J Biol Chem 283:19836-44. 2008
    ..Our results explain how the exchange factor EFA6 via its PH domain could coordinate at the plasma membrane actin cytoskeleton organization with membrane trafficking...
  68. ncbi request reprint Fluorescence correlation spectroscopy analysis of the dynamics of tubulin interaction with RB3, a stathmin family protein
    Tatiana Krouglova
    Laboratory of Biomolecular Dynamics, University of Leuven, Celestijnenlaan 200D, B 3001 Leuven, Belgium
    FEBS Lett 546:365-8. 2003
    ..52+/-0.14)x10(-3) micro;M(-2)/s and (1.9+/-0.6)x10(-3) s(-1) respectively. The value of the equilibrium dissociation constant for the first tubulin-RB3 interaction is estimated to be >or=7 microM at 20 degrees C...
  69. ncbi request reprint Characterization of TccP-mediated N-WASP activation during enterohaemorrhagic Escherichia coli infection
    Junkal Garmendia
    Division of Cellular and Molecular Biology, Imperial College, London, UK
    Cell Microbiol 8:1444-55. 2006
    ..This study defines the modular domain structure of TccP and the molecular basis of TccP-mediated N-WASP activation and EHEC-induced remodelling of the host actin cytoskeleton...
  70. ncbi request reprint TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton
    Junkal Garmendia
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, UK
    Cell Microbiol 6:1167-83. 2004
    ..These results show that EHEC translocates both its own receptor (Tir) and an Nck-like protein (TccP) to facilitate actin polymerization...
  71. pmc Structural basis and evolutionary origin of actin filament capping by twinfilin
    Ville O Paavilainen
    Program in Cellular Biotechnology and Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, FI 00014, Finland
    Proc Natl Acad Sci U S A 104:3113-8. 2007
    ....
  72. doi request reprint Toxoplasma profilin is essential for host cell invasion and TLR11-dependent induction of an interleukin-12 response
    Fabienne Plattner
    Department of Microbiology and Molecular Medicine, CMU, University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    Cell Host Microbe 3:77-87. 2008
    ..Thus, profilin is an essential element of two aspects of T. gondii infection. Like bacterial flagellin, profilin plays a role in motility while serving as a microbial ligand recognized by the host innate immune system...