Sylvie Auxilien

Summary

Affiliation: Avenue de la Terrasse
Country: France

Publications

  1. pmc The human tRNA m (5) C methyltransferase Misu is multisite-specific
    Sylvie Auxilien
    Laboratoire d Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, CNRS, Gif sur Yvette, France
    RNA Biol 9:1331-8. 2012
  2. ncbi request reprint The carboxyl-terminal extension of yeast tRNA m5C methyltransferase enhances the catalytic efficiency of the amino-terminal domain
    Hélène Walbott
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS Bâtiment 34, 1 Avenue de la Terrasse, 91190 Gif sur Yvette, France
    J Biol Chem 282:23663-71. 2007
  3. pmc Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases
    Sylvie Auxilien
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91190 Gif sur Yvette, France
    RNA 17:45-53. 2011
  4. ncbi request reprint Acquisition of a bacterial RumA-type tRNA(uracil-54, C5)-methyltransferase by Archaea through an ancient horizontal gene transfer
    Jaunius Urbonavicius
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 1 Avenue de la Terrasse, 91190, Gif sur Yvette, France
    Mol Microbiol 67:323-35. 2008
  5. pmc Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase
    Hélène Walbott
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette Cedex, France
    RNA 13:967-73. 2007
  6. ncbi request reprint Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase
    Sylvie Auxilien
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Biol Chem 282:18711-21. 2007

Collaborators

Detail Information

Publications6

  1. pmc The human tRNA m (5) C methyltransferase Misu is multisite-specific
    Sylvie Auxilien
    Laboratoire d Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, CNRS, Gif sur Yvette, France
    RNA Biol 9:1331-8. 2012
    ..By comparison, the orthologous yeast enzyme Trm4 catalyzes the methylation of carbon 5 of cytosine at positions 34, 40, 48 or 49 depending on the tRNAs...
  2. ncbi request reprint The carboxyl-terminal extension of yeast tRNA m5C methyltransferase enhances the catalytic efficiency of the amino-terminal domain
    Hélène Walbott
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS Bâtiment 34, 1 Avenue de la Terrasse, 91190 Gif sur Yvette, France
    J Biol Chem 282:23663-71. 2007
    ..It is not required for m(5)C formation and does not appear to contribute to substrate specificity. However, it enhances considerably the catalytic efficiency of the amino-terminal domain...
  3. pmc Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases
    Sylvie Auxilien
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91190 Gif sur Yvette, France
    RNA 17:45-53. 2011
    ..abyssi 23S rRNA. The findings indicate that PAB0719 and PAB0760 originated as RlmD-type m(5)U methyltransferases and underwent changes in target specificity after their acquisition by a Thermococcales ancestor from a bacterial source...
  4. ncbi request reprint Acquisition of a bacterial RumA-type tRNA(uracil-54, C5)-methyltransferase by Archaea through an ancient horizontal gene transfer
    Jaunius Urbonavicius
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 1 Avenue de la Terrasse, 91190, Gif sur Yvette, France
    Mol Microbiol 67:323-35. 2008
    ..This functional shift probably occurred in an ancestor of all Thermococcales. This study also provides new evidence in favour of a close relationship between Thermococcales and Nanoarchaea...
  5. pmc Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase
    Hélène Walbott
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette Cedex, France
    RNA 13:967-73. 2007
    ..Indeed, the enzyme is able to form a stable covalent adduct with the 5-fluorocytosine-containing RNA substrate analog, whereas the C310A mutant protein is inactive and unable to form the covalent complex...
  6. ncbi request reprint Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase
    Sylvie Auxilien
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Biol Chem 282:18711-21. 2007
    ..This P. abyssi system possibly reflects the general function of archeases in preventing protein aggregation and modulating the function of their accompanying proteins...