Research Topics
Genomes and Genes | Jean ArmengaudSummaryCountry: France Publications
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Publications
Identification, purification, and characterization of an eukaryotic-like phosphopantetheine adenylyltransferase (coenzyme A biosynthetic pathway) in the hyperthermophilic archaeon Pyrococcus abyssiJean Armengaud
CEA VALRHO, DSV DIEP, SBTN, Service de Biochimie post génomique and Toxicologie Nucléaire, 30207 Bagnols sur Ceze, France
J Biol Chem 278:31078-87. 2003..The existence of operational proteins highly conserved between Archaea and Eukarya involved in a central metabolic pathway challenge evolutionary scenarios in which eukaryal operational proteins are strictly of bacterial origin...- N2-methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in Archaea and EukaryotaJean Armengaud
Commissariat à l Energie Atomique VALRHO, DSV DIEP SBTN, Service de Biochimie post génomique and Toxicologie Nucléaire, F 30207 Bagnols sur Ceze, France
J Biol Chem 279:37142-52. 2004..The co-occurrence of these two guanosine dimethyltransferases in both Archaea and Eukaryota but not in Bacteria is a hallmark of distinct tRNAs maturation strategies between these domains of life... 
Deciphering structure and topology of conserved COG2042 orphan proteinsJean Armengaud
CEA VALRHO, DSV DIEP SBTN, Service de Biochimie post génomique and Toxicologie Nucléaire, Bagnols sur Ceze, France
BMC Struct Biol 5:3. 2005..As a prototype among COG2042 orthologs, SSO0551 protein from the hyperthermophilic archaeon Sulfolobus solfataricus was purified to homogeneity for biophysical characterization...- Comparative proteogenomics of twelve Roseobacter exoproteomes reveals different adaptive strategies among these marine bacteriaJoseph Alexander Christie-Oleza
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols sur Ceze, F 30207, France
Mol Cell Proteomics 11:M111.013110. 2012..pomeroyi DSS-3 when grown in presence of a natural community sampled from a Mediterranean Sea port. The significance of this specific fraction of the exoproteome is discussed... - Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrateGuillaume Gabant
CEA VALRHO, DSV, IBEB, SBTN, LBSP, Institut de Biologie Environnementale et Biotechnologie, Service de Biochimie et Toxicologie Nucléaire, Laboratoire de Biochimie des Systèmes Perturbés, F 30207 Bagnols sur Ceze, France
J Mass Spectrom 43:360-70. 2008..This method was used to identify two lysine residues of the TrmG10 methyltransferase from Pyrococcus abyssi that were differentially reactive, modified in the protein but not in the tRNA-protein complex... - THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domainGuillaume Gabant
CEA VALRHO, DSV-DIEP-SBTN, , , France
Nucleic Acids Res 34:2483-94. 2006..We propose that this mode of interactions may be common to other THUMP-containing enzymes that specifically modify nucleotides in the 3D-core of tRNA... - Proteomic insights into the lifestyle of an environmentally relevant marine bacteriumJoseph Alexander Christie-Oleza
Laboratoire de Biochimie des Systèmes Perturbés, CEA Marcoule, DSV, IBEB, Bagnols sur Ceze, France
ISME J 6:124-35. 2012..We suggest that the other half of its theoretical proteome is the opportunist genetic pool devoted exclusively to very specific environmental conditions... 
Shotgun nanoLC-MS/MS proteogenomics to document MALDI-TOF biomarkers for screening new members of the Ruegeria genusJoseph Alexander Christie-Oleza
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols sur Ceze, F 30207, France
Environ Microbiol 15:133-47. 2013..Due to its simplicity and effectiveness, this technique could be of immense value in monitoring bacteria in the environment in the near future...- High-throughput proteogenomics of Ruegeria pomeroyi: seeding a better genomic annotation for the whole marine Roseobacter cladeJoseph A Christie-Oleza
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols sur Ceze, F 30207, France
BMC Genomics 13:73. 2012..In this work we applied high-throughput proteogenomics to Ruegeria pomeroyi, a member of the Roseobacter clade, an abundant group of marine bacteria, as a seed for the annotation of the whole clade... - Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus desertiArjan de Groot
Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments LEMiRE, Saint Paul lez Durance, France
PLoS Genet 5:e1000434. 2009..The supplementary nutrient import and DNA repair genes are likely important for survival and adaptation of D. deserti to its nutrient-poor, dry, and UV-exposed extreme environment... - A perfect genome annotation is within reach with the proteomics and genomics allianceJean Armengaud
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols sur Ceze, France
Curr Opin Microbiol 12:292-300. 2009..The proteomics and genomics alliance produces almost complete and accurate gene catalogues for small microbial genomes, a comprehensiveness which is essential for efficient systems biology... - Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiationAlain Dedieu
Laboratoire de Biochimie des Systèmes Perturbés, CEA Marcoule, DSV, IBEB, SBTN, LBSP, Bagnols sur Ceze, F 30207, France
Proteome Sci 11:3. 2013..abstract:.. - Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPNBéatrice Alonso
CEA, IBEB, Service de Biochimie et Toxicologie Nucléaire LBSP, Bagnols sur Ceze, France
Cell Cycle 12:463-72. 2013..These results suggest that all three GPN proteins act at the molecular level in sister chromatid cohesion mechanism as a GPN|GPN complex reminiscent of the homodimeric structure of PAB0955, an archaeal member of GPN-loop GTPase... - Multiple phosphorylable sites in the Zaire Ebolavirus nucleoprotein evidenced by high resolution tandem mass spectrometryJérémy Peyrol
Commissariat a l Energie Atomique, iBEB SBTN LDCAE, Bagnols sur Ceze, France
J Virol Methods 187:159-65. 2013..Because these four phosphorylable sites are conserved among Ebolavirus and Marburgvirus NPs, their modification may play a modulatory role in viral RNA synthesis... - Analytical constraints for the analysis of human cell line secretomes by shotgun proteomicsVeronique Malard
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols sur Ceze, F 30207, France
J Proteomics 75:1043-54. 2012..This study highlights the possible contribution of such proteomic approach in the field of toxicology... - Proteomics-based refinement of Deinococcus deserti genome annotation reveals an unwonted use of non-canonical translation initiation codonsMathieu Baudet
Laboratoire de Biochimie des Systèmes Perturbés, Service de Biochimie et Toxicologie Nucléaire, Institut de Biologie Environnementale et Biotechnologie IBEB, Direction des Sciences du Vivant DSV, Commissariat à l Energie Atomique et aux Energies Alternatives CEA, F 30207 Bagnols sur Ceze, France
Mol Cell Proteomics 9:415-26. 2010..Our results demonstrate that predicting translation initiation codons is still difficult for some bacteria and that proteomics-based refinement of genome annotations may be helpful in such cases... - In-depth analysis of exoproteomes from marine bacteria by shotgun liquid chromatography-tandem mass spectrometry: the Ruegeria pomeroyi DSS-3 case-studyJoseph Alexander Christie-Oleza
Laboratoire de Biochimie des Systèmes Perturbés, CEA Marcoule, DSV, IBEB, SBTN, LBSP, F 30207 Bagnols sur Ceze, France
Mar Drugs 8:2223-39. 2010..The Ruegeria pomeroyi DSS-3 case-study illustrates the power of the shotgun nano-LC-MS/MS strategy to decipher the exoproteome from marine bacteria and to contribute to environmental proteomics... - Exoproteomics: exploring the world around biological systemsJean Armengaud
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols sur Ceze, F 30207, France
Expert Rev Proteomics 9:561-75. 2012..The implication of such data, in terms of proteogenomics and the discovery of novel protein functions, is discussed... - Proteogenomics and systems biology: quest for the ultimate missing partsJean Armengaud
CEA, DSV, IBEB, Lab Biochim System Perturb, CEA Marcoule, DSV, IBEB, SBTN, Laboratoire de Biochimie des Systèmes Perturbés, F 30207 Bagnols sur Ceze, France
Expert Rev Proteomics 7:65-77. 2010..Undoubtedly, comparative genomics will experience a renaissance taking into account this new dimension. Synthetic biology aimed at re-engineering living systems will also benefit from these significant progresses... - A role for GPN-loop GTPase yGPN1 in sister chromatid cohesionBéatrice Alonso
Laboratoire de Biochimie des Systèmes Perturbés, Bagnols sur Ceze, France
Cell Cycle 10:1828-37. 2011..Moreover, analysis of yGPN1 protein-protein interaction network highlights the yGPN1 links with DNA replication, sister chromatid cohesion/separation and the gene expression process... - Identity elements required for enzymatic formation of N2,N2-dimethylguanosine from N2-monomethylated derivative and its possible role in avoiding alternative conformations in archaeal tRNAJaunius Urbonavicius
Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 1 ave de la terrasse, Batiment 34, F-91198 Gif-sur-Yvette, France
J Mol Biol 357:387-99. 2006.... - ApoFnr binds as a monomer to promoters regulating the expression of enterotoxin genes of Bacillus cereusJulia Esbelin
Universite d Avignon, UMR A408, Sécurité et Qualité des Produits d Origine Végétale, and INRA, Avignon, F 84914, France
J Bacteriol 190:4242-51. 2008..The question of whether apoFnr binding is regulated in vivo by redox-dependent oligomerization is discussed... - Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene productStephanie Gras
Laboratoire de Cristallographie et Cristallogenese des Proteines, Institut de Biologie Structurale J P Ebel, CEA CNRS UJF, 41 rue Jules Horowitz, F 38027 Grenoble, France
Acta Crystallogr Sect F Struct Biol Cryst Commun 61:208-11. 2005..Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals... - Determination of estrogen presence in water by SPR using estrogen receptor dimerizationDenis Habauzit
Centre LGEI, Ecole des Mines d Alès, 6 avenue de Clavières, 30319, Ales, France
Anal Bioanal Chem 390:873-83. 2008..We demonstrated that this method allows the direct detection of 17beta-estradiol at concentrations above 1.4 microg/L (5 nM)... - Structure of a [2Fe-2S] ferredoxin from Rhodobacter capsulatus likely involved in Fe-S cluster biogenesis and conformational changes observed upon reductionGermaine Sainz
European Synchrotron Radiation Facility, BP 220, 38054 Grenoble Cedex 9, France
J Biol Inorg Chem 11:235-46. 2006..The unique conformational changes observed in FdVI upon reduction are discussed in the light of structural studies performed on related ferredoxins... - Structural insights into a new homodimeric self-activated GTPase familyStephanie Gras
Laboratoire de Cristallographie et Cristallogenese des Proteines, Institut de Biologie Structurale J P Ebel, CEA CNRS UJF, UMR 5075, 41, rue Jules Horowitz, F 38027 Grenoble, France
EMBO Rep 8:569-75. 2007..Owing to this unique functional feature, we propose to call this family as GPN-loop GTPase...