Research Topics
| Marilyn G WiebeSummaryAffiliation: VTT Biotechnology Country: Finland Publications
| Collaborators
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Detail Information
Publications
Bioconversion of D-galacturonate to keto-deoxy-L-galactonate (3-deoxy-L-threo-hex-2-ulosonate) using filamentous fungiMarilyn G Wiebe
VTT Technical Research Centre of Finland, P, O, Box 1000, FI 02044 VTT, Finland
BMC Biotechnol 10:63. 2010..Keto-deoxy-L-galactonate is a natural intermediate in the fungal D-galacturonate metabolic pathway, and thus keto-deoxy-L-galactonate can be produced in a simple biological conversion...
Lipid production in batch and fed-batch cultures of Rhodosporidium toruloides from 5 and 6 carbon carbohydratesMarilyn G Wiebe
VTT Technical Research Centre of Finland, PO Box 1000, Espoo, FI 02044 VTT, Finland
BMC Biotechnol 12:26. 2012....
Correlation of gene expression and protein production rate - a system wide studyMikko Arvas
VTT Technical Research Centre of Finland, Tietotie 2, P, O, Box FI 1000, 02044 VTT, Espoo, Finland
BMC Genomics 12:616. 2011..Interestingly, it exhibits a low growth rate protein production phenotype...
Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1APaula Jouhten
VTT Technical Research Centre of Finland, Espoo, Finland
BMC Syst Biol 2:60. 2008..Oxygen is a major determinant of the physiology of S. cerevisiae but understanding of the oxygen dependence of intracellular flux distributions is still scarce...
Transcriptional monitoring of steady state and effects of anaerobic phases in chemostat cultures of the filamentous fungus Trichoderma reeseiJari J Rautio
VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P, O, Box 1000, 02044 VTT Espoo, Finland
BMC Genomics 7:247. 2006..Transcriptional responses caused by transient oxygen deprivations and production of foreign protein were also studied in T. reesei by TRAC...
Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditionsMarilyn G Wiebe
VTT Technical Research Centre of Finland, Finland
FEMS Yeast Res 8:140-54. 2008..Gene regulation was more complex, with some genes showing transient upregulation or downregulation during the adaptation to anaerobic conditions...
Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiaeEija Rintala
VTT Technical Research Centre of Finland, P, O, Box 1000, FI 02044 VTT, Finland
BMC Genomics 10:461. 2009..cerevisiae in glucose-limited chemostat cultivations in anaerobic and aerobic conditions, and with three intermediate (0.5, 1.0 and 2.8% oxygen) levels of oxygen in the feed gas...
Engineering filamentous fungi for conversion of D-galacturonic acid to L-galactonic acidJoosu Kuivanen
VTT Technical Research Centre of Finland, Espoo, Finland
Appl Environ Microbiol 78:8676-83. 2012..niger also delayed or prevented induction of the putative d-galacturonate transporter An14g04280. In addition, A. niger ΔgaaB produced l-galactonate from polygalacturonate as efficiently as from the monomer...
Transcription of hexose transporters of Saccharomyces cerevisiae is affected by change in oxygen provisionEija Rintala
VTT, Technical Research Centre of Finland, PO Box 1000, FI 02044 VTT, Finland
BMC Microbiol 8:53. 2008..5, 1, 2.8 and 20.9% O2), and from cells under conditions in which oxygen was introduced to anaerobic cultures or removed from cultures receiving oxygen...
Transcriptional responses of Saccharomyces cerevisiae to shift from respiratory and respirofermentative to fully fermentative metabolismEija Rintala
VTT Technical Research Centre of Finland, Finland
OMICS 15:461-76. 2011....
Metabolic engineering of Saccharomyces cerevisiae for bioconversion of D-xylose to D-xylonateMervi Toivari
VTT, Technical Research Centre of Finland, PO Box 1000, FI 02044 VTT, Espoo, Finland
Metab Eng 14:427-36. 2012..Cell vitality and viability decreased during D-xylonate production at pH 3.0. An industrial S. cerevisiae strain expressing xylB efficiently produced 43 g D-xylonate l(-1) from 49 g D-xylose l(-1)...
Low pH d-xylonate production with Pichia kudriavzeviiMervi Toivari
VTT, Technical Research Centre of Finland, P O Box 1000, FI 02044 VTT, Espoo, Finland Electronic address
Bioresour Technol 133:555-62. 2013..2gL(-1)h(-1) at pH 3.0. This is the best low pH production reported for d-xylonate. These results encourage further development towards industrial scale production...
Saccharomyces cerevisiae engineered to produce D-xylonateMervi H Toivari
VTT, Technical Research Centre of Finland, P O Box 1000, FI 02044 VTT Espoo, Finland
Appl Microbiol Biotechnol 88:751-60. 2010..Although each of these enzymes enhanced NADPH consumption on D-glucose, they did not enhance D-xylonate production, suggesting that NADP+ was not the main limitation in the current D-xylonate producing strains...
Microbial D-xylonate productionMervi H Toivari
VTT, Technical Research Centre of Finland, P O Box 1000, 02044 VTT Espoo, Finland
Appl Microbiol Biotechnol 96:1-8. 2012..oxydans, at a volumetric rate approximately 30% of that observed with G. oxydans. With further development, genetically modified microbes may soon provide an alternative for production of D-xylonate at industrial scale...
Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5Jacob Dam Sørensen
Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, Aalborg, 9000, Denmark
Biotechnol Lett 29:1227-32. 2007..Glucoamylase (165 U l(-1) or 8 mg l(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin...
Acid phosphatase production by Aspergillus niger N402A in continuous flow cultureBudi J Hidayat
Department of Life Sciences, Aalborg University, Aalborg, Denmark
FEMS Microbiol Lett 254:324-31. 2006..Four different ACPs, including two phytases, were produced by A. niger N402A. The ACP and the phytase with maximal activities at pH 5.5 were differentially expressed at different culture pH values, with greater production at low pH...
Rhodotorulic acid production by Rhodotorula mucilaginosaDitte Andersen
Department of Life Sciences, Sohngaardsholmsvej 49, Aalborg University, DK-9000 Aalborg, Denmark
Mycol Res 107:949-56. 2003..Citric acid was an effective suppresser of RA production. RA was produced in a growth rate dependent manner and was optimally produced at pH 6.5...
Development and application of an assay for uranyl complexation by fungal metabolites, including siderophoresJoanna C Renshaw
Centre for Radiochemistry Research, Department of Chemistry, The University of Manchester, Manchester M13 9PT, United Kingdom
Appl Environ Microbiol 69:3600-6. 2003..Our results show that the new assay can be effectively used to screen fungi for the production of UO(2)(2+) chelating ligands. We suggest that hydroxamate siderophores can be produced by mucoraceous fungi...
