Research Topics
Genomes and GenesSpecies | M SaloheimoSummaryAffiliation: VTT Biotechnology Country: Finland Publications
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Detail Information
Publications
cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeastM Saloheimo
VTT Biotechnology and Food Research, Espoo, Finland
Eur J Biochem 249:584-91. 1997..reesei culture supernatant. Northern hybridisation revealed that T. reesei egl4 is regulated in the same manner as other cellulase genes of this fungus...- Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reeseiEmilia Selinheimo
VTT Technical Research Centre of Finland, Espoo, Finland
FEBS J 273:4322-35. 2006..T. reesei TYR2 was active on both l-tyrosine and l-dopa, and it showed broad substrate specificity... 
Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysisJari J Rautio
VTT Technical Research Centre of Finland, Tietotie 2, Espoo, VTT ESPOO, Finland
BMC Biotechnol 7:28. 2007....- Comparison of protein coding gene contents of the fungal phyla Pezizomycotina and SaccharomycotinaMikko Arvas
VTT, Tietotie 2, Espoo, P, O, Box 1500, 02044 VTT, Finland
BMC Genomics 8:325. 2007..We have carried out a computational genome wide comparison of protein coding gene content of Saccharomycotina and Pezizomycotina, which include industrially important yeasts and filamentous fungi, respectively... 
The cargo and the transport system: secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina)Markku Saloheimo
VTT Technical Research Centre of Finland, PO Box 1000, FIN 02044 VTT, Finland
Microbiology 158:46-57. 2012....- Correlation of gene expression and protein production rate - a system wide studyMikko Arvas
VTT Technical Research Centre of Finland, Tietotie 2, P, O, Box FI 1000, 02044 VTT, Espoo, Finland
BMC Genomics 12:616. 2011..Interestingly, it exhibits a low growth rate protein production phenotype... - The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30M Carmen Limón
VTT, P, O, Box 1000, Tietotie 2, Espoo, FIN 02044 VTT, Finland
Microb Cell Fact 10:40. 2011..It has been previously shown that bacterial mutants lacking phosphoglucose isomerase (PGI) produce more nucleotide precursors and amino acids. PGI catalyzes the second step of glycolysis, the formation of fructose-6-P from glucose-6-P... - Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overviewBrigitte Gasser
University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria
Microb Cell Fact 7:11. 2008.... - 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucosePaula Jouhten
VTT Technical Research Centre of Finland, Espoo, Finland
BMC Syst Biol 3:104. 2009..However, T. reesei lacks metabolic pathway reconstructions and the utilization of the respirative pathway has not been investigated on the level of in vivo fluxes... - Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production propertiesMarika Vitikainen
VTT Technical Research Centre of Finland, FI 02044 VTT, Espoo, Finland
BMC Genomics 11:441. 2010.... - Monitoring of transcriptional regulation in Pichia pastoris under protein production conditionsBrigitte Gasser
University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Institute of Applied Microbiology, Vienna, Austria
BMC Genomics 8:179. 2007.... - Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiaeMikko Arvas
VTT Biotechnology, Tietotie 2, Espoo, PL 1500, 02044 VTT, Finland
BMC Genomics 7:32. 2006..This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae... - Transcriptional monitoring of steady state and effects of anaerobic phases in chemostat cultures of the filamentous fungus Trichoderma reeseiJari J Rautio
VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P, O, Box 1000, 02044 VTT Espoo, Finland
BMC Genomics 7:247. 2006..Transcriptional responses caused by transient oxygen deprivations and production of foreign protein were also studied in T. reesei by TRAC... - Eukaryotic protein production in designed storage organellesMargarita Torrent
Departament de Genetica Molecular, Consorci CSIC IRTA, Jordi Girona, Barcelona, Spain
BMC Biol 7:5. 2009..In this work we extend the advantageous properties of plant seed PBs to recombinant protein production in useful non-plant eukaryotic hosts including cultured fungal, mammalian and insect cells... - Exploring laccase-like multicopper oxidase genes from the ascomycete Trichoderma reesei: a functional, phylogenetic and evolutionary studyAnthony Levasseur
UMR 1163, INRA de Biotechnologie des Champignons Filamenteux, IFR86 BAIM, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, CP 925, 13288 Marseille Cedex 09, France
BMC Biochem 11:32. 2010..The purpose of this work was i) to validate the function of a candidate LMCO gene from T. reesei, and ii) to reconstruct LMCO phylogeny and perform evolutionary analysis testing for positive selection... - Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzaeGreta Faccio
VTT Technical Research Centre of Finland PO Box 1000, 02044 Espoo, Finland
BMC Biochem 11:31. 2010.... - Characterization of secretory genes ypt1/yptA and nsf1/nsfA from two filamentous fungi: induction of secretory pathway genes of Trichoderma reesei under secretion stress conditionsMarkku Saloheimo
VTT Biotechnology, Espoo, Finland Genencor International, Inc, Palo Alto, California, USA
Appl Environ Microbiol 70:459-67. 2004..These results suggest the possibility that the whole secretory pathway of T. reesei could be induced at the transcriptional level by stress responses caused by protein accumulation in the secretory pathway... - Activation mechanisms of the HAC1-mediated unfolded protein response in filamentous fungiMarkku Saloheimo
VTT Biotechnology, 02044 VTT, Finland
Mol Microbiol 47:1149-61. 2003..reesei hac1 mRNA is translated more efficiently than a full-length mRNA. This paper reports a novel combination of two different regulatory mechanisms of a transcription factor gene, both operational at the mRNA level... - Enzymatic properties and intracellular localization of the novel Trichoderma reesei beta-glucosidase BGLII (cel1A)Markku Saloheimo
VTT Biotechnology, 02044 VTT, Finland
Appl Environ Microbiol 68:4546-53. 2002..Activity measurements and Western blot analysis of T. reesei strains expressing bgl2 from a constitutive promoter further confirmed the intracellular localization of this beta-glucosidase... - The protein disulphide isomerase gene of the fungus Trichoderma reesei is induced by endoplasmic reticulum stress and regulated by the carbon sourceM Saloheimo
VTT Biotechnology and Food Research, Finland
Mol Gen Genet 262:35-45. 1999..The results suggest that neither sophorose induction nor glucose repression by the CREI protein affect the pdi1 promoter directly... - Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materialsMarkku Saloheimo
VTT Biotechnology, PO Box 1500, 02044 VTT, Finland
Eur J Biochem 269:4202-11. 2002..The SWOI protein was purified from A. niger var. awamori culture supernatant and used in an activity assay with Valonia cell walls. It disrupted the structure of the cell walls without producing detectable amounts of reducing sugars... - Trichoderma reesei rho3 a homologue of yeast RH03 suppresses the growth defect of yeast sec15-1 mutationT Vasara
VTT Biotechnology, Finland
Curr Genet 40:119-27. 2001..Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3 Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype... - The ire1 and ptc2 genes involved in the unfolded protein response pathway in the filamentous fungus Trichoderma reeseiM Valkonen
VTT Biotechnology, PO Box 1500, 02044 VTT, Finland
Mol Genet Genomics 272:443-51. 2004..Splicing of the mRNA encoding the transcription factor HAC1 is also observed. Other genes encoding proteins from different parts of the secretory pathway also respond to ire1 overexpression... - Monitoring the kinetics of glycoprotein synthesis and secretion in the filamentous fungus Trichoderma reesei: cellobiohydrolase I (CBHI) as a model proteinT M Pakula
VTT Biotechnology and Food Research, Finland
Microbiology 146:223-32. 2000..The methodology developed in this study provides tools to reveal the rate-limiting factors in protein production and to obtain information on the intracellular events involved in the secretion process... - Interactions of the Trichoderma reesei rho3 with the secretory pathway in yeast and T. reeseiT Vasara
VTT Biotechnology, PO Box 1500, FIN-02044 VTT Espoo, Finland
Mol Microbiol 42:1349-61. 2001..reesei in cellulose cultures was remarkably decreased in rho3 disruptant strains when compared with the parental strain. These results suggest that rho3 is involved in secretion processes in T. reesei... - Expression of the Trichoderma reesei tyrosinase 2 in Pichia pastoris: isotopic labeling and physicochemical characterizationAnn Westerholm Parvinen
VTT Technical Research Centre of Finland, PO Box 1000, Espoo FIN 02044 VTT, Finland
Protein Expr Purif 55:147-58. 2007..Uniform isotopic (15)N-labeling of TYR2 was carried out with (15)NH(4)SO(4) in minimal medium to assess the suitability of the expression system for investigation by NMR spectroscopy... - Spatially segregated SNARE protein interactions in living fungal cellsMari Valkonen
VTT Biotechnology, P O Box 1000, FIN 02044 VTT, Finland
J Biol Chem 282:22775-85. 2007..This is the first demonstration of spatially regulated SNARE interactions within the same membrane... - Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)Diego Martinez
Los Alamos National Laboratory Joint Genome Institute, PO Box 1663, Los Alamos, New Mexico 87545, USA
Nat Biotechnol 26:553-60. 2008..Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production... - Characterisation of two 14-3-3 genes from Trichoderma reesei: interactions with yeast secretory pathway componentsTuija Vasara
VTT Biotechnology, P.O. Box 1500, Tietotie 2, FIN-02044 VTT, Espoo, Finland
Biochim Biophys Acta 1590:27-40. 2002..These findings strongly suggest that the T. reesei ftt1 has a role in protein secretion... - Production of a chimeric enzyme tool associating the Trichoderma reesei swollenin with the Aspergillus niger feruloyl esterase A for release of ferulic acidAnthony Levasseur
, IFR-IBAIM, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France
Appl Microbiol Biotechnol 73:872-80. 2006..Ferulic acid is used as an antioxidant and flavor precursor in the food and pharmaceutical industries. This is the first report of a potential application of the SWOI protein fused with an enzyme of industrial interest... - Effects of inactivation and constitutive expression of the unfolded- protein response pathway on protein production in the yeast Saccharomyces cerevisiaeMari Valkonen
VTT Biotechnology, Espoo, Finland
Appl Environ Microbiol 69:2065-72. 2003..Overexpression of both S. cerevisiae HAC1 and T. reesei hac1 caused an increase in the expression of the known UPR target gene KAR2 at early time points during cultivation... - cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2Anna Zakrzewska
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, , 02-106 Warsaw, Poland
Curr Genet 43:11-6. 2003..In contrast, expression of the T. reesei cDNA in various S. cerevisiae pmt mutants showed functional similarity to the yeast Pmt2 protein... - The effects of drugs inhibiting protein secretion in the filamentous fungus Trichoderma reesei. Evidence for down-regulation of genes that encode secreted proteins in the stressed cellsTiina M Pakula
VTT Biotechnology, P O Box 1500, 02044 VTT, Espoo, Finland
J Biol Chem 278:45011-20. 2003..By studying expression of the reporter gene lacZ under cbh1 promoters of different length, it was shown that the feedback response was mediated through the cellulase promoter... - Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein responseMari Valkonen
VTT Biotechnology, Espoo, Finland
Appl Environ Microbiol 69:6979-86. 2003..niger var. awamori genes involved in different secretory functions. This revealed both similarities and differences to corresponding studies in Saccharomyces cerevisiae... - Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomycesLaura Leena Kiiskinen
VTT Biotechnology, Espoo, Finland
Appl Environ Microbiol 70:137-44. 2004..The role of the C-terminal extension in laccase production in S. cerevisiae was also studied. Laccase production was increased sixfold with the modified cDNA that had a stop codon after the native processing site at the C terminus... - Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzymeLaura-Leena Kiiskinen
VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Finland
Microbiology 150:3065-74. 2004..The recombinant and wild-type laccases were compared with regard to substrate kinetics, molecular mass, pH optimum, thermostability, and processing of the N- and C-termini, and they showed very similar properties... - Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersoniiPatrick Murray
Molecular Glycobiotechnology Group, Department of Biochemistry, National University of Ireland, Galway, Ireland
Protein Expr Purif 38:248-57. 2004..254 mM). Cel3a was also active against natural cellooligosacharides with glucose being the product of hydrolysis. It displayed transferase activity producing mainly cellobiose from glucose and cellotetrose from cellobiose... - Protein production and induction of the unfolded protein response in Trichoderma reesei strain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tagAnna Collén
Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
Biotechnol Bioeng 89:335-44. 2005..However, only a minor increase in bip1 and pdi1 transcript level was observed in the transformant compared with the parental strain... - Optimization of cDNA-AFLP experiments using genomic sequence dataTeemu Kivioja
Department of Computer Science, University of Helsinki, Helsinki, PO Box 68, FIN 00014, Finland
Bioinformatics 21:2573-9. 2005..The in silico tests performed suggest that substantial amounts of resources can be saved by the optimization of cDNA-AFLP experiments... - Rapid and multiplexed transcript analysis of microbial cultures using capillary electophoresis-detectable oligonucleotide probe poolsJari J Rautio
VTT Biotechnology, Finland
J Microbiol Methods 65:404-16. 2006..g. in gene expression monitoring of production organism in microbial bioprocesses... - Characterization of Melanocarpus albomyces steryl esterase produced in Trichoderma reesei and modification of fibre products with the enzymeHanna Kontkanen
VTT Biotechnology, PO Box 1500, 02044 VTT, Espoo, Finland
Appl Microbiol Biotechnol 72:696-704. 2006..The rSTE1 treatment increased significantly the polarity of PET by hydrolysing the ester bonds in the polyester backbone. Dyeing of PET with methylene blue was also slightly improved after rSTE1 treatment... - Cloning and expression of a Melanocarpus albomyces steryl esterase gene in Pichia pastoris and Trichoderma reeseiHanna Kontkanen
VTT, P O Box 1000, FIN 02044 VTT, Finland
Biotechnol Bioeng 94:407-15. 2006..Triton X-100 was successfully used to recover this activity. The heterologous production system in T. reesei provides a means of producing M. albomyces steryl esterase STE1 reliably in large scale for future studies... - Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper siteNina Hakulinen
Department of Chemistry, University of Joensuu, PO Box 111, FIN-80101, Joensuu, Finland
Nat Struct Biol 9:601-5. 2002..cinereus laccase. In contrast, the C-terminus on the M. albomyces laccase forms a plug that blocks this access... - Overexpression of the gene encoding GTP:mannose-1-phosphate guanyltransferase, mpg1, increases cellular GDP-mannose levels and protein mannosylation in Trichoderma reeseiAnna Zakrzewska
Laboratory of Fungal Glycobiology, Institute of Biochemistry and Biophysics Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Warsaw, Poland
Appl Environ Microbiol 69:4383-9. 2003..Overexpression of the mpg1 gene also increased the transcription of the dpm1 gene and DPMS activity. Our data indicate that the level of cellular GDPMan can play a major regulatory role in protein glycosylation in T. reesei...