M Saloheimo

Summary

Affiliation: VTT Biotechnology
Country: Finland

Publications

  1. ncbi request reprint cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast
    M Saloheimo
    VTT Biotechnology and Food Research, Espoo, Finland
    Eur J Biochem 249:584-91. 1997
  2. ncbi request reprint Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei
    Emilia Selinheimo
    VTT Technical Research Centre of Finland, Espoo, Finland
    FEBS J 273:4322-35. 2006
  3. pmc Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis
    Jari J Rautio
    VTT Technical Research Centre of Finland, Tietotie 2, Espoo, VTT ESPOO, Finland
    BMC Biotechnol 7:28. 2007
  4. pmc Comparison of protein coding gene contents of the fungal phyla Pezizomycotina and Saccharomycotina
    Mikko Arvas
    VTT, Tietotie 2, Espoo, P, O, Box 1500, 02044 VTT, Finland
    BMC Genomics 8:325. 2007
  5. pmc Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates
    Mari Häkkinen
    VTT Technical Research Centre of Finland, Tietotie 2, Espoo, FI 02044, VTT, Finland
    Microb Cell Fact 11:134. 2012
  6. doi request reprint The cargo and the transport system: secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina)
    Markku Saloheimo
    VTT Technical Research Centre of Finland, PO Box 1000, FIN 02044 VTT, Finland
    Microbiology 158:46-57. 2012
  7. pmc Correlation of gene expression and protein production rate - a system wide study
    Mikko Arvas
    VTT Technical Research Centre of Finland, Tietotie 2, P, O, Box FI 1000, 02044 VTT, Espoo, Finland
    BMC Genomics 12:616. 2011
  8. pmc The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30
    M Carmen Limón
    VTT, P, O, Box 1000, Tietotie 2, Espoo, FIN 02044 VTT, Finland
    Microb Cell Fact 10:40. 2011
  9. pmc Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
    Brigitte Gasser
    University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria
    Microb Cell Fact 7:11. 2008
  10. pmc 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose
    Paula Jouhten
    VTT Technical Research Centre of Finland, Espoo, Finland
    BMC Syst Biol 3:104. 2009

Collaborators

Detail Information

Publications45

  1. ncbi request reprint cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast
    M Saloheimo
    VTT Biotechnology and Food Research, Espoo, Finland
    Eur J Biochem 249:584-91. 1997
    ..reesei culture supernatant. Northern hybridisation revealed that T. reesei egl4 is regulated in the same manner as other cellulase genes of this fungus...
  2. ncbi request reprint Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei
    Emilia Selinheimo
    VTT Technical Research Centre of Finland, Espoo, Finland
    FEBS J 273:4322-35. 2006
    ..T. reesei TYR2 was active on both l-tyrosine and l-dopa, and it showed broad substrate specificity...
  3. pmc Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis
    Jari J Rautio
    VTT Technical Research Centre of Finland, Tietotie 2, Espoo, VTT ESPOO, Finland
    BMC Biotechnol 7:28. 2007
    ....
  4. pmc Comparison of protein coding gene contents of the fungal phyla Pezizomycotina and Saccharomycotina
    Mikko Arvas
    VTT, Tietotie 2, Espoo, P, O, Box 1500, 02044 VTT, Finland
    BMC Genomics 8:325. 2007
    ..We have carried out a computational genome wide comparison of protein coding gene content of Saccharomycotina and Pezizomycotina, which include industrially important yeasts and filamentous fungi, respectively...
  5. pmc Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates
    Mari Häkkinen
    VTT Technical Research Centre of Finland, Tietotie 2, Espoo, FI 02044, VTT, Finland
    Microb Cell Fact 11:134. 2012
    ..Plenty of data exists on the carbon source dependent regulation of the characterised hydrolytic genes. However, information on the expression of the novel CAZyme genes, especially on complex biomass material, is very limited...
  6. doi request reprint The cargo and the transport system: secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina)
    Markku Saloheimo
    VTT Technical Research Centre of Finland, PO Box 1000, FIN 02044 VTT, Finland
    Microbiology 158:46-57. 2012
    ....
  7. pmc Correlation of gene expression and protein production rate - a system wide study
    Mikko Arvas
    VTT Technical Research Centre of Finland, Tietotie 2, P, O, Box FI 1000, 02044 VTT, Espoo, Finland
    BMC Genomics 12:616. 2011
    ..Interestingly, it exhibits a low growth rate protein production phenotype...
  8. pmc The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30
    M Carmen Limón
    VTT, P, O, Box 1000, Tietotie 2, Espoo, FIN 02044 VTT, Finland
    Microb Cell Fact 10:40. 2011
    ..It has been previously shown that bacterial mutants lacking phosphoglucose isomerase (PGI) produce more nucleotide precursors and amino acids. PGI catalyzes the second step of glycolysis, the formation of fructose-6-P from glucose-6-P...
  9. pmc Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
    Brigitte Gasser
    University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria
    Microb Cell Fact 7:11. 2008
    ....
  10. pmc 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose
    Paula Jouhten
    VTT Technical Research Centre of Finland, Espoo, Finland
    BMC Syst Biol 3:104. 2009
    ..However, T. reesei lacks metabolic pathway reconstructions and the utilization of the respirative pathway has not been investigated on the level of in vivo fluxes...
  11. pmc Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties
    Marika Vitikainen
    VTT Technical Research Centre of Finland, FI 02044 VTT, Espoo, Finland
    BMC Genomics 11:441. 2010
    ....
  12. pmc Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions
    Brigitte Gasser
    University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Institute of Applied Microbiology, Vienna, Austria
    BMC Genomics 8:179. 2007
    ....
  13. pmc Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae
    Mikko Arvas
    VTT Biotechnology, Tietotie 2, Espoo, PL 1500, 02044 VTT, Finland
    BMC Genomics 7:32. 2006
    ..This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae...
  14. pmc Transcriptional monitoring of steady state and effects of anaerobic phases in chemostat cultures of the filamentous fungus Trichoderma reesei
    Jari J Rautio
    VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P, O, Box 1000, 02044 VTT Espoo, Finland
    BMC Genomics 7:247. 2006
    ..Transcriptional responses caused by transient oxygen deprivations and production of foreign protein were also studied in T. reesei by TRAC...
  15. pmc Eukaryotic protein production in designed storage organelles
    Margarita Torrent
    Departament de Genetica Molecular, Consorci CSIC IRTA, Jordi Girona, Barcelona, Spain
    BMC Biol 7:5. 2009
    ..In this work we extend the advantageous properties of plant seed PBs to recombinant protein production in useful non-plant eukaryotic hosts including cultured fungal, mammalian and insect cells...
  16. pmc Exploring laccase-like multicopper oxidase genes from the ascomycete Trichoderma reesei: a functional, phylogenetic and evolutionary study
    Anthony Levasseur
    UMR 1163, INRA de Biotechnologie des Champignons Filamenteux, IFR86 BAIM, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, CP 925, 13288 Marseille Cedex 09, France
    BMC Biochem 11:32. 2010
    ..The purpose of this work was i) to validate the function of a candidate LMCO gene from T. reesei, and ii) to reconstruct LMCO phylogeny and perform evolutionary analysis testing for positive selection...
  17. pmc Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae
    Greta Faccio
    VTT Technical Research Centre of Finland PO Box 1000, 02044 Espoo, Finland
    BMC Biochem 11:31. 2010
    ....
  18. pmc Characterization of secretory genes ypt1/yptA and nsf1/nsfA from two filamentous fungi: induction of secretory pathway genes of Trichoderma reesei under secretion stress conditions
    Markku Saloheimo
    VTT Biotechnology, Espoo, Finland Genencor International, Inc, Palo Alto, California, USA
    Appl Environ Microbiol 70:459-67. 2004
    ..These results suggest the possibility that the whole secretory pathway of T. reesei could be induced at the transcriptional level by stress responses caused by protein accumulation in the secretory pathway...
  19. ncbi request reprint Activation mechanisms of the HAC1-mediated unfolded protein response in filamentous fungi
    Markku Saloheimo
    VTT Biotechnology, 02044 VTT, Finland
    Mol Microbiol 47:1149-61. 2003
    ..reesei hac1 mRNA is translated more efficiently than a full-length mRNA. This paper reports a novel combination of two different regulatory mechanisms of a transcription factor gene, both operational at the mRNA level...
  20. pmc Enzymatic properties and intracellular localization of the novel Trichoderma reesei beta-glucosidase BGLII (cel1A)
    Markku Saloheimo
    VTT Biotechnology, 02044 VTT, Finland
    Appl Environ Microbiol 68:4546-53. 2002
    ..Activity measurements and Western blot analysis of T. reesei strains expressing bgl2 from a constitutive promoter further confirmed the intracellular localization of this beta-glucosidase...
  21. ncbi request reprint The protein disulphide isomerase gene of the fungus Trichoderma reesei is induced by endoplasmic reticulum stress and regulated by the carbon source
    M Saloheimo
    VTT Biotechnology and Food Research, Finland
    Mol Gen Genet 262:35-45. 1999
    ..The results suggest that neither sophorose induction nor glucose repression by the CREI protein affect the pdi1 promoter directly...
  22. ncbi request reprint Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials
    Markku Saloheimo
    VTT Biotechnology, PO Box 1500, 02044 VTT, Finland
    Eur J Biochem 269:4202-11. 2002
    ..The SWOI protein was purified from A. niger var. awamori culture supernatant and used in an activity assay with Valonia cell walls. It disrupted the structure of the cell walls without producing detectable amounts of reducing sugars...
  23. ncbi request reprint Trichoderma reesei rho3 a homologue of yeast RH03 suppresses the growth defect of yeast sec15-1 mutation
    T Vasara
    VTT Biotechnology, Finland
    Curr Genet 40:119-27. 2001
    ..Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3 Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype...
  24. ncbi request reprint The ire1 and ptc2 genes involved in the unfolded protein response pathway in the filamentous fungus Trichoderma reesei
    M Valkonen
    VTT Biotechnology, PO Box 1500, 02044 VTT, Finland
    Mol Genet Genomics 272:443-51. 2004
    ..Splicing of the mRNA encoding the transcription factor HAC1 is also observed. Other genes encoding proteins from different parts of the secretory pathway also respond to ire1 overexpression...
  25. ncbi request reprint Monitoring the kinetics of glycoprotein synthesis and secretion in the filamentous fungus Trichoderma reesei: cellobiohydrolase I (CBHI) as a model protein
    T M Pakula
    VTT Biotechnology and Food Research, Finland
    Microbiology 146:223-32. 2000
    ..The methodology developed in this study provides tools to reveal the rate-limiting factors in protein production and to obtain information on the intracellular events involved in the secretion process...
  26. ncbi request reprint Interactions of the Trichoderma reesei rho3 with the secretory pathway in yeast and T. reesei
    T Vasara
    VTT Biotechnology, PO Box 1500, FIN-02044 VTT Espoo, Finland
    Mol Microbiol 42:1349-61. 2001
    ..reesei in cellulose cultures was remarkably decreased in rho3 disruptant strains when compared with the parental strain. These results suggest that rho3 is involved in secretion processes in T. reesei...
  27. ncbi request reprint Expression of the Trichoderma reesei tyrosinase 2 in Pichia pastoris: isotopic labeling and physicochemical characterization
    Ann Westerholm-Parvinen
    VTT Technical Research Centre of Finland, PO Box 1000, Espoo FIN 02044 VTT, Finland
    Protein Expr Purif 55:147-58. 2007
    ..Uniform isotopic (15)N-labeling of TYR2 was carried out with (15)NH(4)SO(4) in minimal medium to assess the suitability of the expression system for investigation by NMR spectroscopy...
  28. ncbi request reprint Spatially segregated SNARE protein interactions in living fungal cells
    Mari Valkonen
    VTT Biotechnology, P O Box 1000, FIN 02044 VTT, Finland
    J Biol Chem 282:22775-85. 2007
    ..This is the first demonstration of spatially regulated SNARE interactions within the same membrane...
  29. doi request reprint Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)
    Diego Martinez
    Los Alamos National Laboratory Joint Genome Institute, PO Box 1663, Los Alamos, New Mexico 87545, USA
    Nat Biotechnol 26:553-60. 2008
    ..Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production...
  30. ncbi request reprint Characterisation of two 14-3-3 genes from Trichoderma reesei: interactions with yeast secretory pathway components
    Tuija Vasara
    VTT Biotechnology, P O Box 1500, Tietotie 2, FIN 02044 VTT, Espoo, Finland
    Biochim Biophys Acta 1590:27-40. 2002
    ..These findings strongly suggest that the T. reesei ftt1 has a role in protein secretion...
  31. ncbi request reprint Production of a chimeric enzyme tool associating the Trichoderma reesei swollenin with the Aspergillus niger feruloyl esterase A for release of ferulic acid
    Anthony Levasseur
    UMR 1163 INRA Universités de Provence et de la Méditerranée de Biotechnologie des Champignons Filamenteux, IFR IBAIM, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France
    Appl Microbiol Biotechnol 73:872-80. 2006
    ..Ferulic acid is used as an antioxidant and flavor precursor in the food and pharmaceutical industries. This is the first report of a potential application of the SWOI protein fused with an enzyme of industrial interest...
  32. pmc Effects of inactivation and constitutive expression of the unfolded- protein response pathway on protein production in the yeast Saccharomyces cerevisiae
    Mari Valkonen
    VTT Biotechnology, Espoo, Finland
    Appl Environ Microbiol 69:2065-72. 2003
    ..Overexpression of both S. cerevisiae HAC1 and T. reesei hac1 caused an increase in the expression of the known UPR target gene KAR2 at early time points during cultivation...
  33. ncbi request reprint cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2
    Anna Zakrzewska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Curr Genet 43:11-6. 2003
    ..In contrast, expression of the T. reesei cDNA in various S. cerevisiae pmt mutants showed functional similarity to the yeast Pmt2 protein...
  34. ncbi request reprint The effects of drugs inhibiting protein secretion in the filamentous fungus Trichoderma reesei. Evidence for down-regulation of genes that encode secreted proteins in the stressed cells
    Tiina M Pakula
    VTT Biotechnology, P O Box 1500, 02044 VTT, Espoo, Finland
    J Biol Chem 278:45011-20. 2003
    ..By studying expression of the reporter gene lacZ under cbh1 promoters of different length, it was shown that the feedback response was mediated through the cellulase promoter...
  35. pmc Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response
    Mari Valkonen
    VTT Biotechnology, Espoo, Finland
    Appl Environ Microbiol 69:6979-86. 2003
    ..niger var. awamori genes involved in different secretory functions. This revealed both similarities and differences to corresponding studies in Saccharomyces cerevisiae...
  36. pmc Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomyces
    Laura Leena Kiiskinen
    VTT Biotechnology, Espoo, Finland
    Appl Environ Microbiol 70:137-44. 2004
    ..The role of the C-terminal extension in laccase production in S. cerevisiae was also studied. Laccase production was increased sixfold with the modified cDNA that had a stop codon after the native processing site at the C terminus...
  37. ncbi request reprint Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme
    Laura Leena Kiiskinen
    VTT Biotechnology, PO Box 1500, FIN 02044 VTT, Finland
    Microbiology 150:3065-74. 2004
    ..The recombinant and wild-type laccases were compared with regard to substrate kinetics, molecular mass, pH optimum, thermostability, and processing of the N- and C-termini, and they showed very similar properties...
  38. ncbi request reprint Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii
    Patrick Murray
    Molecular Glycobiotechnology Group, Department of Biochemistry, National University of Ireland, Galway, Ireland
    Protein Expr Purif 38:248-57. 2004
    ..254 mM). Cel3a was also active against natural cellooligosacharides with glucose being the product of hydrolysis. It displayed transferase activity producing mainly cellobiose from glucose and cellotetrose from cellobiose...
  39. ncbi request reprint Protein production and induction of the unfolded protein response in Trichoderma reesei strain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tag
    Anna Collén
    Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
    Biotechnol Bioeng 89:335-44. 2005
    ..However, only a minor increase in bip1 and pdi1 transcript level was observed in the transformant compared with the parental strain...
  40. ncbi request reprint Optimization of cDNA-AFLP experiments using genomic sequence data
    Teemu Kivioja
    Department of Computer Science, University of Helsinki, Helsinki, PO Box 68, FIN 00014, Finland
    Bioinformatics 21:2573-9. 2005
    ..Our aim was to test whether this workload can be reduced by rational design of the experiment...
  41. ncbi request reprint Rapid and multiplexed transcript analysis of microbial cultures using capillary electophoresis-detectable oligonucleotide probe pools
    Jari J Rautio
    VTT Biotechnology, Finland
    J Microbiol Methods 65:404-16. 2006
    ..g. in gene expression monitoring of production organism in microbial bioprocesses...
  42. ncbi request reprint Characterization of Melanocarpus albomyces steryl esterase produced in Trichoderma reesei and modification of fibre products with the enzyme
    Hanna Kontkanen
    VTT Biotechnology, PO Box 1500, 02044 VTT, Espoo, Finland
    Appl Microbiol Biotechnol 72:696-704. 2006
    ..The rSTE1 treatment increased significantly the polarity of PET by hydrolysing the ester bonds in the polyester backbone. Dyeing of PET with methylene blue was also slightly improved after rSTE1 treatment...
  43. ncbi request reprint Cloning and expression of a Melanocarpus albomyces steryl esterase gene in Pichia pastoris and Trichoderma reesei
    Hanna Kontkanen
    VTT, P O Box 1000, FIN 02044 VTT, Finland
    Biotechnol Bioeng 94:407-15. 2006
    ..Triton X-100 was successfully used to recover this activity. The heterologous production system in T. reesei provides a means of producing M. albomyces steryl esterase STE1 reliably in large scale for future studies...
  44. ncbi request reprint Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site
    Nina Hakulinen
    Department of Chemistry, University of Joensuu, PO Box 111, FIN 80101, Joensuu, Finland
    Nat Struct Biol 9:601-5. 2002
    ..cinereus laccase. In contrast, the C-terminus on the M. albomyces laccase forms a plug that blocks this access...
  45. pmc Overexpression of the gene encoding GTP:mannose-1-phosphate guanyltransferase, mpg1, increases cellular GDP-mannose levels and protein mannosylation in Trichoderma reesei
    Anna Zakrzewska
    Laboratory of Fungal Glycobiology, Institute of Biochemistry and Biophysics Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Warsaw, Poland
    Appl Environ Microbiol 69:4383-9. 2003
    ..Overexpression of the mpg1 gene also increased the transcription of the dpm1 gene and DPMS activity. Our data indicate that the level of cellular GDPMan can play a major regulatory role in protein glycosylation in T. reesei...