Johanna Vuojola

Summary

Affiliation: University of Turku
Country: Finland

Publications

  1. doi Genetically encoded protease substrate based on lanthanide-binding peptide for time-gated fluorescence detection
    Johanna Vuojola
    Department of Biotechnology, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
    Anal Chem 85:1367-73. 2013
  2. doi Distance and temperature dependency in nonoverlapping and conventional Förster resonance energy-transfer
    Johanna Vuojola
    Department of Biotechnology, University of Turku, FI 20520 Turku, Finland
    J Phys Chem B 115:13685-94. 2011
  3. doi Fluorescence-quenching-based homogeneous caspase-3 activity assay using photon upconversion
    Johanna Vuojola
    Department of Biotechnology, University of Turku, Turku, Finland
    Anal Chim Acta 725:67-73. 2012
  4. doi Resonance energy transfer from lanthanide chelates to overlapping and nonoverlapping fluorescent protein acceptors
    Johanna Vuojola
    Department of Biotechnology, University of Turku, FI 20520 Turku, Finland
    Anal Chem 81:5033-8. 2009
  5. doi Upconverting phosphors in a dual-parameter LRET-based hybridization assay
    Terhi Rantanen
    Department of Biotechnology, University of Turku, Tykistökatu 6A 6th floor, 20520 Turku, Finland
    Analyst 134:1713-6. 2009
  6. doi Fluorescence-quenching-based enzyme-activity assay by using photon upconversion
    Terhi Rantanen
    Department of Biotechnology, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Angew Chem Int Ed Engl 47:3811-3. 2008

Detail Information

Publications6

  1. doi Genetically encoded protease substrate based on lanthanide-binding peptide for time-gated fluorescence detection
    Johanna Vuojola
    Department of Biotechnology, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
    Anal Chem 85:1367-73. 2013
    ..By varying the fluorescent protein and the protease specificity of the internal linker sequence, the method can be applied for the detection of a wide variety of proteases...
  2. doi Distance and temperature dependency in nonoverlapping and conventional Förster resonance energy-transfer
    Johanna Vuojola
    Department of Biotechnology, University of Turku, FI 20520 Turku, Finland
    J Phys Chem B 115:13685-94. 2011
    ..On the basis of our measurements, we propose that in nFRET thermal excitation occurs from the lowest radiative state of the ion to a higher excited state that is either ionic or associated with a ligand-to-metal charge-transfer state...
  3. doi Fluorescence-quenching-based homogeneous caspase-3 activity assay using photon upconversion
    Johanna Vuojola
    Department of Biotechnology, University of Turku, Turku, Finland
    Anal Chim Acta 725:67-73. 2012
    ..By further utilizing assay miniaturization and signal enhancement in a white microtitration plate, a significant reduction in the reagent consumption can be achieved rendering the assay applicable for high-throughput screening...
  4. doi Resonance energy transfer from lanthanide chelates to overlapping and nonoverlapping fluorescent protein acceptors
    Johanna Vuojola
    Department of Biotechnology, University of Turku, FI 20520 Turku, Finland
    Anal Chem 81:5033-8. 2009
    ..The lanthanide chelates together with fluorescent proteins enable a straightforward and sensitive assay technology in nFRET applications...
  5. doi Upconverting phosphors in a dual-parameter LRET-based hybridization assay
    Terhi Rantanen
    Department of Biotechnology, University of Turku, Tykistökatu 6A 6th floor, 20520 Turku, Finland
    Analyst 134:1713-6. 2009
    ..35-5.4 nM). The UCPs enable multiplexed homogeneous LRET-based assay requiring only a single excitation wavelength, which simplifies the detection and extends the applicability of upconversion in bioanalytical measurements...
  6. doi Fluorescence-quenching-based enzyme-activity assay by using photon upconversion
    Terhi Rantanen
    Department of Biotechnology, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Angew Chem Int Ed Engl 47:3811-3. 2008