Kalervo Vaananen

Summary

Affiliation: University of Turku
Country: Finland

Publications

  1. pmc Analysis by siRNA_profile program displays novel thermodynamic characteristics of highly functional siRNA molecules
    Pirkko Muhonen
    Institute of Biomedicine, Department of Anatomy, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Source Code Biol Med 3:8. 2008
  2. ncbi request reprint Mechanism of osteoclast mediated bone resorption--rationale for the design of new therapeutics
    Kalervo Vaananen
    Department of Anatomy, Institute of Biomedicine, University of Turku, FI 20700 Turku, Finland
    Adv Drug Deliv Rev 57:959-71. 2005
  3. doi request reprint Osteoclast lineage and function
    H Kalervo Vaananen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FI 20520, Turku, Finland
    Arch Biochem Biophys 473:132-8. 2008
  4. ncbi request reprint Mesenchymal stem cells
    H Kalervo Vaananen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland
    Ann Med 37:469-79. 2005
  5. ncbi request reprint The cell biology of osteoclast function
    H K Vaananen
    Skeletal Research Program, Department of Anatomy, Institute of Biomedicine, University of Turku, Finland
    J Cell Sci 113:377-81. 2000
  6. ncbi request reprint Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+
    P Lehenkari
    Department of Anatomy, University of Oulu, Finland
    Exp Cell Res 242:128-37. 1998
  7. doi request reprint Neurofibromatosis 1-related osteopenia often progresses to osteoporosis in 12 years
    Eetu Heervä
    Department of Cell Biology and Anatomy, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland
    Calcif Tissue Int 92:23-7. 2013
  8. doi request reprint Osteoclasts derived from patients with neurofibromatosis 1 (NF1) display insensitivity to bisphosphonates in vitro
    Eetu Heervä
    University of Turku, Department of Cell Biology and Anatomy, Turku 20520, Finland
    Bone 50:798-803. 2012
  9. pmc Differential effects of selective oestrogen receptor modulators (SERMs) tamoxifen, ospemifene and raloxifene on human osteoclasts in vitro
    H Michael
    Department of Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland
    Br J Pharmacol 151:384-95. 2007
  10. doi request reprint Phenotypic characterization of transgenic mice harboring Nf1+/- or Nf1-/- osteoclasts in otherwise Nf1+/+ background
    Maria H Alanne
    Department of Cell Biology and Anatomy, University of Turku, and Department of Dermatology, Turku University Hospital, Turku, Finland
    J Cell Biochem 113:2136-46. 2012

Collaborators

Detail Information

Publications28

  1. pmc Analysis by siRNA_profile program displays novel thermodynamic characteristics of highly functional siRNA molecules
    Pirkko Muhonen
    Institute of Biomedicine, Department of Anatomy, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Source Code Biol Med 3:8. 2008
    ..Here we report the improved results of a new siRNA design program and analysis tool called siRNA_profile that reveals an additional criterion for bioinformatic search of highly functional siRNA sequences...
  2. ncbi request reprint Mechanism of osteoclast mediated bone resorption--rationale for the design of new therapeutics
    Kalervo Vaananen
    Department of Anatomy, Institute of Biomedicine, University of Turku, FI 20700 Turku, Finland
    Adv Drug Deliv Rev 57:959-71. 2005
    ..Thus we are expecting several new bone inhibitors to be developed in following years both for experimental studies and finally also for clinical use...
  3. doi request reprint Osteoclast lineage and function
    H Kalervo Vaananen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FI 20520, Turku, Finland
    Arch Biochem Biophys 473:132-8. 2008
    ..Detailed molecular knowledge of osteoclast differentiation and function has helped us to identify several target molecules and develop specific treatments to inhibit pathological bone resorption in various skeletal diseases...
  4. ncbi request reprint Mesenchymal stem cells
    H Kalervo Vaananen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland
    Ann Med 37:469-79. 2005
    ..However, much new research is needed to learn enough on the molecular mechanisms of MSC differentiation to evaluate their full capacity for tissue regeneration...
  5. ncbi request reprint The cell biology of osteoclast function
    H K Vaananen
    Skeletal Research Program, Department of Anatomy, Institute of Biomedicine, University of Turku, Finland
    J Cell Sci 113:377-81. 2000
    ..It also facilitates further processing of the degradation products intracellularly during the passage through the cell...
  6. ncbi request reprint Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+
    P Lehenkari
    Department of Anatomy, University of Oulu, Finland
    Exp Cell Res 242:128-37. 1998
    ..0. We conclude that CA II is essential not only in bone resorption but also in osteoclast differentiation. In both processes, however, the crucial role of CA II is at least partially due to the effect on the osteoclast pHi regulation...
  7. doi request reprint Neurofibromatosis 1-related osteopenia often progresses to osteoporosis in 12 years
    Eetu Heervä
    Department of Cell Biology and Anatomy, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland
    Calcif Tissue Int 92:23-7. 2013
    ..Even though our sample size was 19 patients, we recommend follow-up of NF1 patients with osteopenia and consideration of prophylactic measures to prevent osteoporosis and associated fracture risk...
  8. doi request reprint Osteoclasts derived from patients with neurofibromatosis 1 (NF1) display insensitivity to bisphosphonates in vitro
    Eetu Heervä
    University of Turku, Department of Cell Biology and Anatomy, Turku 20520, Finland
    Bone 50:798-803. 2012
    ..Ras-inhibitor FTS counteracted this NF1-related insensitivity to zoledronic acid, suggesting that Ras may play a role in this phenomenon...
  9. pmc Differential effects of selective oestrogen receptor modulators (SERMs) tamoxifen, ospemifene and raloxifene on human osteoclasts in vitro
    H Michael
    Department of Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland
    Br J Pharmacol 151:384-95. 2007
    ..Here, we have investigated the effects of different types of SERMs on osteoclast differentiation, bone resorption and apoptosis in vitro...
  10. doi request reprint Phenotypic characterization of transgenic mice harboring Nf1+/- or Nf1-/- osteoclasts in otherwise Nf1+/+ background
    Maria H Alanne
    Department of Cell Biology and Anatomy, University of Turku, and Department of Dermatology, Turku University Hospital, Turku, Finland
    J Cell Biochem 113:2136-46. 2012
    ....
  11. pmc Resorption-cycle-dependent polarization of mRNAs for different subunits of V-ATPase in bone-resorbing osteoclasts
    T Laitala-Leinonen
    Department of Anatomy, University of Oulu, Finland
    Mol Biol Cell 7:129-42. 1996
    ..Antisense RNA against the 16-kDa or 60-kDa V-ATPase subunit inhibits polarization of the osteoclasts, as determined by cytoskeleton staining. Antisense RNA against carbonic anhydrase II, however, has no such effect...
  12. doi request reprint Inactivation of estrogen receptor α in bone-forming cells induces bone loss in female mice
    Jorma A Maatta
    Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, FI20520 Turku, Finland
    FASEB J 27:478-88. 2013
    ..bv...
  13. ncbi request reprint Conditioned medium of estrogen-treated osteoblasts inhibits osteoclast maturation and function in vitro
    Q Qu
    Department of Anatomy, Institute of Biomedicine and Medicity Research Laboratory, University of Turku, Finland
    Bone 25:211-5. 1999
    ..1 nmol/L E2. Our data suggest that E2 treatment of osteoblasts decreases the production of factor(s) that induces osteoclast differentiation to multinucleated cells with a higher capacity for bone resorption...
  14. ncbi request reprint Serum tartrate-resistant acid phosphatase 5b (TRACP 5b) as a marker of skeletal changes in prostate cancer
    Eeva Salminen
    Department of Oncology, Turku University Hospital, Finland
    Acta Oncol 44:742-7. 2005
    ..TRACP 5b was less specific than tALP as a marker of skeletal metastases. TRACP 5b may have a role in the diagnostics of skeletal changes in PC with a focus on treatment-related skeletal changes...
  15. ncbi request reprint Inhibition of intravacuolar acidification by antisense RNA decreases osteoclast differentiation and bone resorption in vitro
    T Laitala-Leinonen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Finland
    J Cell Sci 112:3657-66. 1999
    ..These results suggest that CA II and V-ATPase are needed, not only for the actual resorption, but also for osteoclast formation in vitro...
  16. ncbi request reprint Characterization of circulating human osteoclast progenitors: development of in vitro resorption assay
    M Husheem
    Department of Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Calcif Tissue Int 76:222-30. 2005
    ..This assay was shown to be functional, since two well-known resorption inhibitors, bafilomycin A(1) and calcitonin, dose-dependently inhibited the resorption activity of osteoclasts...
  17. ncbi request reprint Comparative effects of estrogen and antiestrogens on differentiation of osteoblasts in mouse bone marrow culture
    Q Qu
    Department of Anatomy, Institute of Biomedicine and Medicity Research Laboratory, University of Turku, Finland
    J Cell Biochem 73:500-7. 1999
    ..Our results show that E2 as well as TAM, FC1271a, and RAL stimulate bone formation in vitro but the mechanism of the anabolic action of RAL in bone clearly differs from that of E2, TAM, and FC1271a...
  18. ncbi request reprint Rapid screening method for osteoclast differentiation in vitro that measures tartrate-resistant acid phosphatase 5b activity secreted into the culture medium
    S L Alatalo
    Institute of Biomedicine, Department of Anatomy, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Clin Chem 46:1751-4. 2000
    ..Osteoclasts secrete tartrate-resistant acid phosphatase (TRAP; EC 3.1.3.2) 5b into the circulation. We studied the release of TRAP 5b from osteoclasts using a mouse in vitro osteoclast differentiation assay...
  19. ncbi request reprint Serum TRACP 5b and ICTP as markers of bone metastases in breast cancer
    J Korpela
    Department of Oncology, Turku University Hospital, Turku, Finland
    Anticancer Res 26:3127-32. 2006
    ....
  20. ncbi request reprint Tartrate-resistant acid phosphatase facilitates hydroxyl radical formation and colocalizes with phagocytosed Staphylococcus aureus in alveolar macrophages
    S R Räisänen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland
    Biochem Biophys Res Commun 288:142-50. 2001
    ..These results suggest that TRAP may have an important biological function in the defence mechanism of macrophages by generating intracellular ROS which would be targeted to destroy phagocytosed foreign material...
  21. ncbi request reprint Serum tartrate-resistant acid phosphatase 5b, but not 5a, correlates with other markers of bone turnover and bone mineral density
    J M Halleen
    Institute of Biomedicine, Department of Anatomy, University of Turku, Turku, Finland
    Calcif Tissue Int 71:20-5. 2002
    ..These results show that serum TRACP 5b, but not 5a, reflects the bone resorption rate, and that our TRACP 5b immunoassay may be a specific method for the determination of the bone resorption rate from serum samples...
  22. ncbi request reprint Periapical cemental dysplasia is common in women with NF1
    Vivian Visnapuu
    Department of Anatomy, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Eur J Med Genet 50:274-80. 2007
    ..Neurofibromatosis type 1 (NF1) is a genetic disorder with skeletal involvement. Periapical cemental dysplasia is a rare finding in the normal population...
  23. pmc Alendronate decreases orthotopic PC-3 prostate tumor growth and metastasis to prostate-draining lymph nodes in nude mice
    Johanna M Tuomela
    Institute of Biomedicine, Department of Anatomy, University of Turku, Finland
    BMC Cancer 8:81. 2008
    ..We used a modified orthotopic PC-3 nude mouse tumor model of human prostate cancer to study whether alendronate affects prostate tumor growth and metastasis...
  24. ncbi request reprint Downregulation of small GTPase Rab7 impairs osteoclast polarization and bone resorption
    H Zhao
    Department of Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    J Biol Chem 276:39295-302. 2001
    ..The results indicate that the late endocytotic pathway is involved in the osteoclast polarization and bone resorption and underscore the importance of Rab7 in osteoclast function...
  25. ncbi request reprint The regulation of pHi in osteoclasts is dependent on the culture substrate and on the stage of the resorption cycle
    P P Lehenkari
    Department of Anatomy, University of Oulu, Finland
    Biochem Biophys Res Commun 235:838-44. 1997
    ..In contrast, as little as 10(-10) M bafilomycin A1 caused accumulation of large vesicles in the cytoplasm...
  26. ncbi request reprint Differential regulation of carbonic anhydrase II by androgen and estrogen in dorsal and lateral prostate of the rat
    P L Harkonen
    Department of Anatomy, University of Turku, Finland
    Endocrinology 128:3219-27. 1991
    ..Estrogen increases CA II expression in lateral prostate but in dorsal prostate the castration-like effects of estrogen on CAII expression are probably indirect...
  27. doi request reprint Osteoclasts in neurofibromatosis type 1 display enhanced resorption capacity, aberrant morphology, and resistance to serum deprivation
    Eetu Heervä
    University of Turku, Department of Cell Biology and Anatomy, Kiinamyllynkatu 10, Turku 20520, Finland
    Bone 47:583-90. 2010
    ..Thus, increased osteoclast activity may be a phenotypic property of the NF1 syndrome, and at least in part explain selected skeletal findings in NF1, such as osteoporosis/osteopenia...
  28. ncbi request reprint Development and evaluation of three immunofluorometric assays that measure different forms of osteocalcin in serum
    S M Käkönen
    Department of Biotechnology, University of Turku, FIN 20520 Turku, Finland
    Clin Chem 46:332-7. 2000
    ..Circulating human osteocalcin (hOC) has been used as a marker of bone formation. Our aim was to validate three immunofluorometric assays (IFMAs), measuring different forms of hOC...