Affiliation: University of Tampere
- Testing of nine different xeno-free culture media for human embryonic stem cell culturesKristiina Rajala
REGEA, Institute for Regenerative Medicine, University of Tampere, Tampere University Hospital, Tampere, Finland
Hum Reprod 22:1231-8. 2007..However, currently used culture conditions contain animal-derived components that bear a risk of transmitting animal pathogens and incorporation of non-human immunogenic molecules to hESC...
- A defined and xeno-free culture method enabling the establishment of clinical-grade human embryonic, induced pluripotent and adipose stem cellsKristiina Rajala
Regea Institute for Regenerative Medicine, University of Tampere, Tampere University Hospital, Tampere, Finland
PLoS ONE 5:e10246. 2010..For clinical application of stem cells, the use of completely defined conditions and elimination of all animal-derived materials from the establishment, culture, and differentiation processes is desirable...
- Culture conditions affect cardiac differentiation potential of human pluripotent stem cellsMarisa Ojala
Institute of Biomedical Technology, University of Tampere, Tampere, Finland
PLoS ONE 7:e48659. 2012..In conclusion, culture conditions have a major impact on the propensity of the hPSCs to differentiate into a cardiac lineage...
- Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methodsSusanna Narkilahti
REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, 33520 Tampere, Finland
Biomed Eng Online 6:11. 2007..Continuous monitoring of living cell cultures can reveal more information and is faster than using laborious traditional methods such as microscopic evaluation, immunohistochemistry and flow cytometry...
- Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cellsHanna Vaajasaari
Regea Institute for Regenerative Medicine, University of Tampere, Tampere, Finland
Mol Vis 17:558-75. 2011....
- The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cellsMari Pekkanen-Mattila
Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
Stem Cells Int 2012:875059. 2012..In conclusion, the cells behaved differently whether cultured on hFF or MEF feeders. However, the influence of the feeder cells on differentiation was less than the difference observed between the cell lines...
- Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell linesHeidi Hakala
REGEA, Institute for Regenerative Medicine, University of Tampere, Tampere, Finland
Tissue Eng Part A 15:1775-85. 2009..A long-term, feeder cell-free culture system was successful on Matrigel in combination with mTeSR1 culture medium, but a xeno-free, fully defined, and reproducible feeder cell-free hESC culture method still remains to be developed...
- Cardiac differentiation of pluripotent stem cellsKristiina Rajala
Regea Institute for Regenerative Medicine, University of Tampere, Tampere University Hospital, 33520 Tampere, Finland
Stem Cells Int 2011:383709. 2011..In this paper, we will discuss the strategies for the generation of cardiomyocytes from pluripotent stem cells and their characteristics, as well as highlight some applications for the cells...