Arthur Ouwehand

Summary

Affiliation: University of Turku
Country: Finland

Publications

  1. ncbi request reprint Measurement of bacterial adhesion-in vitro evaluation of different methods
    Satu Vesterlund
    Department of Biochemistry and Food Chemistry, University of Turku, Itäinen Pitkäkatu 4A, 20014 Turku, Finland
    J Microbiol Methods 60:225-33. 2005
  2. ncbi request reprint Antiallergic effects of probiotics
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry and Functional Foods Forum, University of Turku, 20014 Turku, Finland
    J Nutr 137:794S-7S. 2007
  3. ncbi request reprint Phenotypic differences between commercial Lactobacillus rhamnosus GG and L. rhamnosus strains recovered from blood
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Clin Infect Dis 39:1858-60. 2004
  4. ncbi request reprint Use of a probiotic Bifidobacterium in a dry food matrix, an in vivo study
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, and Functional Foods Forum, University of Turku, 20014 Turku, Finland
    Int J Food Microbiol 95:103-6. 2004
  5. ncbi request reprint Microbiota composition of the intestinal mucosa: association with fecal microbiota?
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland
    Microbiol Immunol 48:497-500. 2004
  6. pmc Disease-dependent adhesion of lactic acid bacteria to the human intestinal mucosa
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Clin Diagn Lab Immunol 10:643-6. 2003
  7. ncbi request reprint Health aspects of probiotics
    Arthur Ouwehand
    University of Turku, Department of Biochemistry and Food Chemistry, 20014 Turku, Finland
    IDrugs 6:573-80. 2003
  8. ncbi request reprint Probiotics for the skin: a new area of potential application?
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Lett Appl Microbiol 36:327-31. 2003
  9. ncbi request reprint The role of the intestinal microflora for the development of the immune system in early childhood
    Arthur Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Eur J Nutr 41:I32-7. 2002
  10. ncbi request reprint Probiotics: an overview of beneficial effects
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Antonie Van Leeuwenhoek 82:279-89. 2002

Collaborators

Detail Information

Publications50

  1. ncbi request reprint Measurement of bacterial adhesion-in vitro evaluation of different methods
    Satu Vesterlund
    Department of Biochemistry and Food Chemistry, University of Turku, Itäinen Pitkäkatu 4A, 20014 Turku, Finland
    J Microbiol Methods 60:225-33. 2005
    ..As a result, we show that the best reproducibility and sensitivity were obtained using radioactive labelling. With other methods, the sensitivity was too low due to poorly adhering bacteria and low signal-to-background ratio...
  2. ncbi request reprint Antiallergic effects of probiotics
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry and Functional Foods Forum, University of Turku, 20014 Turku, Finland
    J Nutr 137:794S-7S. 2007
    ..Another important area will be the investigation of interactions between probiotics and other food components that influence allergies. This will enable optimization of probiotic use for the allergic subject...
  3. ncbi request reprint Phenotypic differences between commercial Lactobacillus rhamnosus GG and L. rhamnosus strains recovered from blood
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Clin Infect Dis 39:1858-60. 2004
    ..We compared the virulence potential of these clinical isolates with that of the probiotic strain. It was observed that all isolates differed significantly in 1 or more phenotypic properties...
  4. ncbi request reprint Use of a probiotic Bifidobacterium in a dry food matrix, an in vivo study
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, and Functional Foods Forum, University of Turku, 20014 Turku, Finland
    Int J Food Microbiol 95:103-6. 2004
    ..One week after cessation of B. lactis Bb-12 feeding, it could be identified in the faeces of five of the nine subjects...
  5. ncbi request reprint Microbiota composition of the intestinal mucosa: association with fecal microbiota?
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland
    Microbiol Immunol 48:497-500. 2004
    ..This indicates that the unidentified part of the microbiota, especially on the mucosa, deserves more attention...
  6. pmc Disease-dependent adhesion of lactic acid bacteria to the human intestinal mucosa
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Clin Diagn Lab Immunol 10:643-6. 2003
    ..rhamnosus strain GG, displayed disease-specific adhesion to intestinal mucus. These results suggest that strains with optimal binding characteristics for a particular intestinal disease can be selected...
  7. ncbi request reprint Health aspects of probiotics
    Arthur Ouwehand
    University of Turku, Department of Biochemistry and Food Chemistry, 20014 Turku, Finland
    IDrugs 6:573-80. 2003
    ..Other beneficial health effects have also been attributed to probiotics; however, these have not been sufficiently substantiated. Additional research into the mechanisms of probiotics is required...
  8. ncbi request reprint Probiotics for the skin: a new area of potential application?
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Lett Appl Microbiol 36:327-31. 2003
    ..The current study aimed at assessing, in vitro, the potential use of probiotics for the skin...
  9. ncbi request reprint The role of the intestinal microflora for the development of the immune system in early childhood
    Arthur Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Eur J Nutr 41:I32-7. 2002
    ..The precise mechanisms behind these immune modulatory activities are not well understood and require further investigation...
  10. ncbi request reprint Probiotics: an overview of beneficial effects
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Antonie Van Leeuwenhoek 82:279-89. 2002
    ..Results from well conducted clinical studies will expand and increase the acceptance of probiotics for the treatment and prevention of selected diseases...
  11. ncbi request reprint Effect of probiotics on constipation, fecal azoreductase activity and fecal mucin content in the elderly
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Ann Nutr Metab 46:159-62. 2002
    ..Constipation is a common problem in elderly subjects, probiotics have been suggested to improve intestinal motility and reduce fecal enzyme activity...
  12. ncbi request reprint The mucus binding of Bifidobacterium lactis Bb12 is enhanced in the presence of Lactobacillus GG and Lact. delbrueckii subsp. bulgaricus
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Lett Appl Microbiol 30:10-3. 2000
    ..The results suggest that combinations of probiotics strains may have synergistic adhesion effects. Such specific strain combinations should also be assessed in clinical studies...
  13. ncbi request reprint Wheat or rye supplemented diets do not affect faecal mucus concentration or the adhesion of probiotic micro-organisms to faecal mucus
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Lett Appl Microbiol 31:30-3. 2000
    ....
  14. ncbi request reprint The normal faecal microflora does not affect the adhesion of probiotic bacteria in vitro
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    FEMS Microbiol Lett 177:35-8. 1999
    ..This validates the results of earlier reports where the influence of the normal microflora was not taken into account...
  15. ncbi request reprint Adhesion of inactivated probiotic strains to intestinal mucus
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, and Turku, Finland
    Lett Appl Microbiol 31:82-6. 2000
    ..Inactivation by u.v. was not observed to modulate the adhesion of the tested strains and it was concluded to be the most appropriate method for studying non-viable probiotics and preparing control products...
  16. ncbi request reprint Assessment of adhesion properties of novel probiotic strains to human intestinal mucus
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Int J Food Microbiol 64:119-26. 2001
    ..The growth media and the food matrix significantly affect the adhesive ability of the tested strains. This has previously not been taken into account when selecting novel probiotic strains...
  17. pmc Resected human colonic tissue: new model for characterizing adhesion of lactic acid bacteria
    Arthur C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Clin Diagn Lab Immunol 9:184-6. 2002
    ..The adhesion of commonly used probiotic strains to human intestinal tissue pieces and mucus was assessed. The strains tested adhered to the intestinal tissue at low levels and adhered to the intestinal mucus at higher levels...
  18. ncbi request reprint Adhesion of four Bifidobacterium strains to human intestinal mucus from subjects in different age groups
    A C Ouwehand
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    FEMS Microbiol Lett 172:61-4. 1999
    ..The results suggest that reduced adhesion may be a factor involved in the decreasing colonisation of elderly subjects by bifidobacteria...
  19. ncbi request reprint Inventing probiotic functional foods for patients with allergic disease
    Kirsi Laiho
    Department of Paediatrics, University of Turku, Turku, Finland
    Ann Allergy Asthma Immunol 89:75-82. 2002
    ..The primary objective of this review is to discuss the rationale for and applicability of probiotics as part of the nutritional approach to the prevention and management of allergic disease...
  20. ncbi request reprint The hygiene hypothesis of atopic disease--an extended version
    Samuli Rautava
    Department of Pediatrics, Turku University Central Hospital, Finland
    J Pediatr Gastroenterol Nutr 38:378-88. 2004
    ..Third, in addition to protection against atopy, protection against infectious, inflammatory, and autoimmune diseases may also depend upon healthy host-microbe interactions implicated in the hygiene hypothesis...
  21. ncbi request reprint Safety assessment of Lactobacillus strains: presence of putative risk factors in faecal, blood and probiotic isolates
    Satu Vesterlund
    Department of Biochemistry and Food Chemistry and Functional Foods Forum, University of Turku, 20014 Turku, Finland
    Int J Food Microbiol 116:325-31. 2007
    ..However, the significance of adhesion to mucus, low induction of respiratory burst in PMN and resistance to human serum-mediated killing may need further evaluation in experimental animal models and in epidemiological data...
  22. pmc Probiotic bacteria may become dormant during storage
    Sampo J Lahtinen
    Functional Foods Forum University of Turku, Itainen Pitkakatu 4, 20014 Turku, Finland
    Appl Environ Microbiol 71:1662-3. 2005
    ..A subpopulation of nonculturable cells retained a functional cell membrane typical of viable cells, indicating that probiotic bacteria become dormant during storage...
  23. ncbi request reprint Adhesion of bacteria to resected human colonic tissue: quantitative analysis of bacterial adhesion and viability
    Satu Vesterlund
    Department of Biochemistry and Food Chemistry, University of Turku, Itäinen Pitkäkatu 4A, 20014 Turku, Finland
    Res Microbiol 156:238-44. 2005
    ..rhamnosus GG in displacement assays. Moreover, the method is suitable for studies in which the interaction of two or more bacteria is examined in an environment in which other bacteria are present...
  24. ncbi request reprint Prebiotics and other microbial substrates for gut functionality
    Arthur C Ouwehand
    Danisco Innovation, Sokeritehtaantie 20, FIN 02460 Kantvik, Finland
    Curr Opin Biotechnol 16:212-7. 2005
    ..Novel approaches might include the application of prebiotics in different combinations or the provision of nutrients to different bacterial groups and to different parts of the intestine...
  25. ncbi request reprint Novel approaches to the nutritional management of the allergic infant
    Erika Isolauri
    Department of Paediatrics, University of Turku, Turku, Finland
    Acta Paediatr Suppl 94:110-4. 2005
    ....
  26. doi request reprint Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial
    Damien Paineau
    Nutri Health, Rueil Malmaison, France
    FEMS Immunol Med Microbiol 53:107-13. 2008
    ..Specific strains of probiotics may thus act as adjuvants to the humoral immune response following oral vaccination...
  27. ncbi request reprint Effect of orally administered non-viable Lactobacillus cells on murine humoral immune responses
    Fang He
    Technical Research Laboratory, Takanashi Milk Products Co, Ltd, Yokohama, Kanagawa, Japan
    Microbiol Immunol 49:993-7. 2005
    ..01). These results suggest that the non-viable Lactobacillus GG can augment the systemic and mucosal immune responses in a host animal favoring secretory IgA but not IgE in an adjuvant-like manner...
  28. ncbi request reprint Staphylococcus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria
    Satu Vesterlund
    Functional Foods Forum, Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Microbiology 152:1819-26. 2006
    ..This study shows for the first time that Staph. aureus can adhere to human intestinal mucus and adherent bacteria can be displaced and killed by certain LAB strains via in situ production of antimicrobial substances...
  29. pmc Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays
    Sampo J Lahtinen
    Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland
    Appl Environ Microbiol 72:5132-4. 2006
    ..The strains showed reduction in plate counts but were able to maintain esterase activity, intact cytoplasmic membrane, and pH gradient. The apparently uncultivable probiotic cells were active and stress resistant...
  30. doi request reprint Bifidobacterium microbiota and parameters of immune function in elderly subjects
    Arthur C Ouwehand
    Functional Foods Forum, University of Turku, Turku, Finland
    FEMS Immunol Med Microbiol 53:18-25. 2008
    ..This indicates that modulation of the faecal Bifidobacterium microbiota may provide a means of influencing inflammatory responses...
  31. ncbi request reprint Effects of lactose on colon microbial community structure and function in a four-stage semi-continuous culture system
    Harri A Mäkivuokko
    Danisco Innovation, Kantvik, Finland
    Biosci Biotechnol Biochem 70:2056-63. 2006
    ..In conclusion, lactose produced dramatic changes in microbiota composition and activity as compared with the baseline fermentation...
  32. ncbi request reprint Specific Bifidobacterium strains isolated from elderly subjects inhibit growth of Staphylococcus aureus
    Sampo J Lahtinen
    Functional Foods Forum, Department of Food Chemistry and Biochemistry, University of Turku, 20520 Turku, Finland
    Int J Food Microbiol 117:125-8. 2007
    ..However, specific strains were identified which showed considerable inhibitory activity against S. aureus...
  33. ncbi request reprint Comparison of four methods to enumerate probiotic bifidobacteria in a fermented food product
    Sampo J Lahtinen
    Functional Foods Forum, Department of Food Chemistry and Biochemistry, University of Turku, 20520 Turku, Finland
    Food Microbiol 23:571-7. 2006
    ..The strain-specific properties and the objects of the analysis should be taken into account when enumeration methods for different probiotic strains are chosen...
  34. ncbi request reprint Streptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs
    Minna L Rinkinen
    Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, P O Box 57, University of Helsinki, Finland
    FEMS Microbiol Lett 230:35-9. 2004
    ..According to these results, Streptococcus alactolyticus was the dominant culturable LAB species in both feces and jejunal chyme. In addition, Lactobacillus murinus and Lactobacillus reuteri were detected...
  35. ncbi request reprint Interaction between probiotic lactic acid bacteria and canine enteric pathogens: a risk factor for intestinal Enterococcus faecium colonization?
    Minna Rinkinen
    Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, P O Box 57, Helsinki FIN 00014, Finland
    Vet Microbiol 92:111-9. 2003
    ..Enhanced C. jejuni adhesion is a new potential risk factor of enterococci. Our results further emphasize the importance of safety guidelines to be established for the probiotics intended for animal use...
  36. ncbi request reprint Performance of bifidobacteria in oat-based media
    Riikka Laine
    Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Int J Food Microbiol 83:105-9. 2003
    ..However, their adhesion to colonic tissue was significantly lower. Prior exposure to the oat-based medium increased the adhesion of two of the strains...
  37. ncbi request reprint Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli
    Yuan Kun Lee
    Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    J Med Microbiol 52:925-30. 2003
    ..These findings are important for development, selection and in vitro assessment of target- and function-specific probiotics...
  38. ncbi request reprint Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus)
    Sami Nikoskelainen
    Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland
    Fish Shellfish Immunol 15:443-52. 2003
    ..Serum immunoglobulin levels were significantly raised only in LAB8 group on week 1 and in LAB4 and LAB8 at the end of the trial. The results show that rainbow trout immune parameters were enhanced by using probiotic bacteria...
  39. ncbi request reprint Absence of host specificity for in vitro adhesion of probiotic lactic acid bacteria to intestinal mucus
    Minna Rinkinen
    Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, P O Box 57, FIN 00014 Helsinki, Finland
    Vet Microbiol 97:55-61. 2003
    ..Positive health effects facilitated by adherent probiotics in humans may also denote the possibility of similar outcome in other species and vice versa...
  40. ncbi request reprint Selection of enterococci for potential canine probiotic additives
    Viola Strompfová
    Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4 6, 04001 Kosice, Slovak Republic
    Vet Microbiol 100:107-14. 2004
    ..E. faecalis EE4 and E. faecium EF01 showed the best probiotic properties. It indicates that they could be used as new candidate probiotic strains after in vivo testing...
  41. ncbi request reprint In vivo safety assessment of two Bifidobacterium longum strains
    Henna Mäkeläinen
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Microbiol Immunol 47:911-4. 2003
    ..Based on this study it appears that the two strains are well tolerated by human subjects and there are no reservations about their food use...
  42. ncbi request reprint Rapid screening method for the detection of antimicrobial substances
    Satu Vesterlund
    Department of Biochemistry and Food Chemistry, University of Turku, Itäinen Pitkäkatu 4A, 20014 Turku, Finland
    J Microbiol Methods 57:23-31. 2004
    ..Furthermore, the method was used for the detection of antimicrobial activity from lactic acid bacteria after the effect of organic acids was eliminated...
  43. ncbi request reprint Microbial-gut interactions in health and disease. Probiotics
    Erika Isolauri
    Department of Paediatrics, Turku University Central Hospital, Kiinamyllynkatu 4 8, 20520 Turku, Finland
    Best Pract Res Clin Gastroenterol 18:299-313. 2004
    ..However, further rigorous scientific efforts are required to characterize the immunomodulatory potential of specific probiotic strains for these targets...
  44. doi request reprint Potential of enterococci isolated from horses
    Andrea Lauková
    Institute of Animal Physiology Slovak Academy of Sciences, Soltesovej 4 6, 04001 Kosice, Slovakia
    Anaerobe 14:234-6. 2008
    ..Three suitable candidates-the strains of E. faecium EF 412, EF 462 and EF 491 were selected for further detail studies and possibilities to be used as additives...
  45. ncbi request reprint Cytokine production by the murine macrophage cell line J774.1 after exposure to lactobacilli
    Hirotsugu Morita
    Technical Research Laboratory, Takanashi Milk Products Co, Ltd, Yokohama, Kanagawa, Japan
    Biosci Biotechnol Biochem 66:1963-6. 2002
    ..0001; ANOVA). These results suggest that lactobacilli can activate macrophages to secrete both inflammatory and anti-inflammatory cytokines. Selected strains might be used to bring about pro or antiinflammatory immune reactions...
  46. ncbi request reprint Adhesion of lactic acid bacteria to caco-2 cells and their effect on cytokine secretion
    Hirotsugu Morita
    Technical Research Laboratory, Takanashi Milk Products Co, Ltd, Yokohama, Kanagawa, Japan
    Microbiol Immunol 46:293-7. 2002
    ..This indicates that lactic acid bacteria, even those with strong adhesive properties, are not very likely to trigger an inflammatory response in human enterocytes...
  47. ncbi request reprint Stimulation of the secretion of pro-inflammatory cytokines by Bifidobacterium strains
    Fang He
    Technical Research Laboratory Takanashi Milk Products Co, Ltd, Yokohama, Kanagawa 241 0023, Japan
    Microbiol Immunol 46:781-5. 2002
    ..1 cells as the other tested bacteria did. The results suggest that the adult-type bifidobacteria, especially B. adolescentis, may be more potent to amplify but less able to down-regulate the inflammatory response...
  48. ncbi request reprint Adhesion of vancomycin-resistant enterococcus to human intestinal mucus
    Nicole J Pultz
    Research Section, Louis Stokes Cleveland Veterans Affairs Medical Center, Case Western Reserve University, Cleveland, OH, 44106, USA
    Curr Microbiol 52:221-4. 2006
    ..rhamnosus GG demonstrated 20- to 130-times higher affinity than the VREF strains. These results demonstrate that VREF strains may adhere to human intestinal mucus and suggest that L. rhamnosus GG might be able to displace VREF strains...
  49. ncbi request reprint Probiotics: time to move beyond Metchnikoff?
    Arthur C Ouwehand
    Drug Discov Today 8:1063. 2003
  50. ncbi request reprint Intestinal Bifidobacterium species induce varying cytokine production
    Fang He
    J Allergy Clin Immunol 109:1035-6. 2002