J K Hiltunen

Summary

Affiliation: University of Oulu
Country: Finland

Publications

  1. ncbi request reprint Peroxisomal membrane permeability and solute transfer
    Vasily D Antonenkov
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O Box 3000, FIN 90014 Oulu, Finland
    Biochim Biophys Acta 1763:1697-706. 2006
  2. ncbi request reprint The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae
    J Kalervo Hiltunen
    Biocenter Oulu and Department of Biochemistry, P O Box 3000, FIN 90014 University of Oulu, Oulu, Finland
    FEMS Microbiol Rev 27:35-64. 2003
  3. doi request reprint Mitochondrial fatty acid synthesis--an adopted set of enzymes making a pathway of major importance for the cellular metabolism
    J Kalervo Hiltunen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O B 3000, FI 90014 Oulu, Finland
    Prog Lipid Res 49:27-45. 2010
  4. pmc Mitochondrial fatty acid synthesis type II: more than just fatty acids
    J Kalervo Hiltunen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, FI 90014 Oulu, Finland
    J Biol Chem 284:9011-5. 2009
  5. doi request reprint Mitochondrial fatty acid synthesis and respiration
    J Kalervo Hiltunen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, PO Box 3000, FI 90014 Oulu, Finland
    Biochim Biophys Acta 1797:1195-202. 2010
  6. ncbi request reprint Mitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast
    J K Hiltunen
    Biocenter Oulu and Department of Biochemistry, P O B 3000, University of Oulu, FIN 90014 Oulu, Finland
    Biochem Soc Trans 33:1162-5. 2005
  7. ncbi request reprint beta-oxidation - strategies for the metabolism of a wide variety of acyl-CoA esters
    J K Hiltunen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O FIN 90014, Oulun yliopisto, Finland
    Biochim Biophys Acta 1484:117-28. 2000
  8. ncbi request reprint An ancient genetic link between vertebrate mitochondrial fatty acid synthesis and RNA processing
    Kaija J Autio
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O Box 3000, FIN 90014 Oulu, Finland
    FASEB J 22:569-78. 2008
  9. ncbi request reprint Human peroxisomal multifunctional enzyme type 2. Site-directed mutagenesis studies show the importance of two protic residues for 2-enoyl-CoA hydratase 2 activity
    Y M Qin
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Box 3000, FIN 90014 Oulun Yliopisto, Finland
    J Biol Chem 275:4965-72. 2000
  10. ncbi request reprint Peroxisomal beta-oxidation and polyunsaturated fatty acids
    J K Hiltunen
    Biocenter Oulu, University of Oulu, Finland
    Ann N Y Acad Sci 804:116-28. 1996

Collaborators

Detail Information

Publications67

  1. ncbi request reprint Peroxisomal membrane permeability and solute transfer
    Vasily D Antonenkov
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O Box 3000, FIN 90014 Oulu, Finland
    Biochim Biophys Acta 1763:1697-706. 2006
    ..The possible involvement of the channels in the transfer of small metabolites and in the formation of peroxisomal shuttle systems is described...
  2. ncbi request reprint The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae
    J Kalervo Hiltunen
    Biocenter Oulu and Department of Biochemistry, P O Box 3000, FIN 90014 University of Oulu, Oulu, Finland
    FEMS Microbiol Rev 27:35-64. 2003
    ....
  3. doi request reprint Mitochondrial fatty acid synthesis--an adopted set of enzymes making a pathway of major importance for the cellular metabolism
    J Kalervo Hiltunen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O B 3000, FI 90014 Oulu, Finland
    Prog Lipid Res 49:27-45. 2010
    ..The first publications linking mitochondrial FAS II to disease states in mammals are emerging...
  4. pmc Mitochondrial fatty acid synthesis type II: more than just fatty acids
    J Kalervo Hiltunen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, FI 90014 Oulu, Finland
    J Biol Chem 284:9011-5. 2009
    ..The intersection of mitochondrial FAS and RNA metabolism in both systems provides a novel mechanism for the coordination of intermediary metabolism in eukaryotic cells...
  5. doi request reprint Mitochondrial fatty acid synthesis and respiration
    J Kalervo Hiltunen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, PO Box 3000, FI 90014 Oulu, Finland
    Biochim Biophys Acta 1797:1195-202. 2010
    ..The linkage of FAS II genes to published animal models for human disease supports the hypothesis that mitochondrial FAS dysfunction leads to the development of disorders in mammals...
  6. ncbi request reprint Mitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast
    J K Hiltunen
    Biocenter Oulu and Department of Biochemistry, P O B 3000, University of Oulu, FIN 90014 Oulu, Finland
    Biochem Soc Trans 33:1162-5. 2005
    ..Finally, Hfa1p was shown to be the mitochondrial acetyl-CoA carboxylase...
  7. ncbi request reprint beta-oxidation - strategies for the metabolism of a wide variety of acyl-CoA esters
    J K Hiltunen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O FIN 90014, Oulun yliopisto, Finland
    Biochim Biophys Acta 1484:117-28. 2000
    ....
  8. ncbi request reprint An ancient genetic link between vertebrate mitochondrial fatty acid synthesis and RNA processing
    Kaija J Autio
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O Box 3000, FIN 90014 Oulu, Finland
    FASEB J 22:569-78. 2008
    ....
  9. ncbi request reprint Human peroxisomal multifunctional enzyme type 2. Site-directed mutagenesis studies show the importance of two protic residues for 2-enoyl-CoA hydratase 2 activity
    Y M Qin
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Box 3000, FIN 90014 Oulun Yliopisto, Finland
    J Biol Chem 275:4965-72. 2000
    ..The data show that the hydratase 2 reaction catalyzed by MFE-2 requires two protic residues, Glu-366 and Asp-510, suggesting that their catalytic role may be equivalent to that of the two catalytic residues of hydratase 1...
  10. ncbi request reprint Peroxisomal beta-oxidation and polyunsaturated fatty acids
    J K Hiltunen
    Biocenter Oulu, University of Oulu, Finland
    Ann N Y Acad Sci 804:116-28. 1996
    ....
  11. ncbi request reprint Response of SCP-2L domain of human MFE-2 to ligand removal: binding site closure and burial of peroxisomal targeting signal
    M F Lensink
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O Box 3000, FIN 90014, Oulu, Finland
    J Mol Biol 323:99-113. 2002
    ..The results are in accordance with plant nsLTPs, where a similar accommodation of binding pocket size was found after ligand binding/removal. Furthermore, the calculations support the suggestion of a ligand-assisted targeting mechanism...
  12. ncbi request reprint Peroxisomal bifunctional protein from rat liver is a trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities
    P M Palosaari
    Department of Medical Biochemistry, University of Oulu, Finland
    J Biol Chem 265:2446-9. 1990
    ..2.1.17), and L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) activities in the same polypeptide...
  13. ncbi request reprint The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase
    A M Mursula
    Biocenter Oulu and Department of Biochemistry, University of Oulu, FIN-90014, Finland
    J Mol Biol 309:845-53. 2001
    ....
  14. ncbi request reprint Yeast peroxisomal multifunctional enzyme: (3R)-hydroxyacyl-CoA dehydrogenase domains A and B are required for optimal growth on oleic acid
    Y M Qin
    Biocenter Oulu, Department of Biochemistry, University of Oulu, FIN 90570 Oulu, Finland
    J Biol Chem 274:28619-25. 1999
    ..The data show that the dehydrogenase domains of yeast MFE-2 have different substrate specificities required to allow the yeast to propagate optimally on fatty acids as the carbon source...
  15. ncbi request reprint Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 A resolution
    A M Haapalainen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, FIN 90014, Finland
    J Mol Biol 313:1127-38. 2001
    ..In addition, the C-terminal peroxisomal targeting signal is ordered in the structure and solvent-exposed, which is not the case with unliganded rabbit SCP-2, supporting the hypothesis of a ligand-assisted targeting mechanism...
  16. ncbi request reprint In mouse alpha -methylacyl-CoA racemase, the same gene product is simultaneously located in mitochondria and peroxisomes
    T J Kotti
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, Oulu FIN 90014, Finland
    J Biol Chem 275:20887-95. 2000
    ..It is concluded that the noncleavable N-terminal sequence of the polypeptide acts as a weak mitochondrial and that the C-terminal sequence acts as a peroxisomal targeting signal...
  17. ncbi request reprint Htd2p/Yhr067p is a yeast 3-hydroxyacyl-ACP dehydratase essential for mitochondrial function and morphology
    Alexander J Kastaniotis
    Biocenter Oulu, Department of Biochemistry, University of Oulu, FIN 90014 Oulu, Finland
    Mol Microbiol 53:1407-21. 2004
    ..The mitochondrial phenotypes of the null and overexpression mutants suggest a crucial role of YHR067w in maintenance of mitochondrial respiratory competence and morphology in yeast...
  18. ncbi request reprint The rat liver peroxisomal membrane forms a permeability barrier for cofactors but not for small metabolites in vitro
    Vasily D Antonenkov
    Department of Biochemistry and Biocenter Oulu, University of Oulu, PO Box 3000, FI 90014, Oulu, Finland
    J Cell Sci 117:5633-42. 2004
    ..The results also indicate that molecular size plays an important role in in vivo distinction between cofactors and metabolic intermediates...
  19. ncbi request reprint Structural biology of the thioester-dependent degradation and synthesis of fatty acids
    Prasenjit Bhaumik
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, PO Box 3000, FIN 90014 Oulu, Finland
    Curr Opin Struct Biol 15:621-8. 2005
    ..Current structural knowledge of the enzymes comprising these pathways shows that the approximately 130 entries in the RCSB Protein Data Bank can be grouped into seven superfamilies. Multifunctional enzymes are important in both pathways...
  20. ncbi request reprint Delta3,5-delta2,4-dienoyl-CoA isomerase from rat liver. Molecular characterization
    S A Filppula
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, FIN 90570 Oulu, Finland
    J Biol Chem 273:349-55. 1998
    ..Transport of the protein into the mitochondria with cleavage of the targeting signal results in a mature mitochondrial form with a molecular mass of 32,000 Da; transport to peroxisomes yields a protein of 36,000 Da...
  21. pmc Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites
    Prasad Kasaragod
    Biocenter Oulu and Department of Biochemistry, University of Oulu, FI 90014 Oulu, Finland
    J Biol Chem 285:24089-98. 2010
    ....
  22. pmc Localization of a portion of the liver isoform of fatty-acid-binding protein (L-FABP) to peroxisomes
    Vasily D Antonenkov
    Department of Biochemistry, Biocenter Oulu, University of Oulu, Linnanmaa, P O Box 3000, FIN 90014 Oulu, Finland
    Biochem J 394:475-84. 2006
    ..The possible role of intraperoxisomal L-FABP in lipid metabolism is discussed...
  23. ncbi request reprint The yeast mitochondrial proteome, a study of fermentative and respiratory growth
    Steffen Ohlmeier
    Biocenter Oulu and Department of Biochemistry, P O Box 3000, University of Oulu, Oulu FIN 90014, Finland
    J Biol Chem 279:3956-79. 2004
    ..We propose that the discrepancy between proteome and transcriptional regulation, apart from different translation efficiency, indicates a changed turnover rate of proteins in different physiological conditions...
  24. ncbi request reprint UK114, a YjgF/Yer057p/UK114 family protein highly conserved from bacteria to mammals, is localized in rat liver peroxisomes
    Vasily D Antonenkov
    Department of Biochemistry, Biocenter Oulu, University of Oulu, Linnanmaa, P O Box 3000, FIN 90014 Oulu, Finland
    Biochem Biophys Res Commun 357:252-7. 2007
    ..The peroxisomal UK114 is a soluble matrix protein and it is not inducible by the peroxisomal proliferator clofibrate. The data predict involvement of UK114 in peroxisomal metabolism...
  25. doi request reprint Protein phosphorylation in mitochondria --a study on fermentative and respiratory growth of Saccharomyces cerevisiae
    Steffen Ohlmeier
    Proteomics Core Facility, Biocenter Oulu, and Department of Biochemistry, University of Oulu, Oulu, Finland
    Electrophoresis 31:2869-81. 2010
    ..g. glucose fermentation, tricarboxylic acid cycle, pyruvate dehydrogenase and its bypass) and respiratory chain...
  26. doi request reprint The 3-hydroxyacyl-ACP dehydratase of mitochondrial fatty acid synthesis in Trypanosoma brucei
    Kaija J Autio
    Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland
    FEBS Lett 582:729-33. 2008
    ..TbHTD2 can rescue the respiratory deficient phenotype of the yeast knock-out strain and restore the lipoic acid content, is localized in the mitochondrion and exhibits hydratase 2 activity...
  27. doi request reprint An involvement of yeast peroxisomal channels in transmembrane transfer of glyoxylate cycle intermediates
    Vasily D Antonenkov
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O Box 3000, FIN 90014, Oulu, Finland
    Int J Biochem Cell Biol 41:2546-54. 2009
    ..The channel inducible by DTT facilitates the transfer of solutes with a molecular mass up to 400Da, sufficient to allow the transmembrane trafficking of glyoxylate cycle intermediates between the peroxisomal lumen and the cytoplasm...
  28. doi request reprint 17beta-hydroxysteroid dehydrogenase type 8 and carbonyl reductase type 4 assemble as a ketoacyl reductase of human mitochondrial FAS
    Zhijun Chen
    Biocenter Oulu, and Department of Biochemistry, P O Box 3000, FI 90014 University of Oulu, Finland
    FASEB J 23:3682-91. 2009
    ..Notably, 17beta-HSD8 was previously classified as a steroid-metabolizing enzyme, but our data suggest that 17beta-HSD8 is primarily involved in mitochondrial FAS...
  29. ncbi request reprint The behavior of peroxisomes in vitro: mammalian peroxisomes are osmotically sensitive particles
    Vasily D Antonenkov
    Department of Biochemistry and Biocenter Oulu, University of Oulu, PO Box 3000, FIN 90014 Oulu, Finland
    Am J Physiol Cell Physiol 287:C1623-35. 2004
    ..g., polyethylene glycol 1500. A method was developed for the isolation of highly purified and nearly intact peroxisomes from rat liver by using polyethylene glycol 1500 as an osmoprotector...
  30. ncbi request reprint A novel mutation of the fumarase gene in a family with autosomal recessive fumarase deficiency
    Anne M Remes
    Department of Neurology, University of Oulu, Oulu, Finland
    J Mol Med (Berl) 82:550-4. 2004
    ..Analysis of the FH mutation and the mutant enzyme variant described here provides an explanation for the mechanism of FH deficiency at the molecular level and paves the way for the analysis of other dysfunctional FH variants...
  31. ncbi request reprint Structure-function analysis of enoyl thioester reductase involved in mitochondrial maintenance
    Tomi T Airenne
    Biocenter Oulu and Department of Biochemistry, P O Box 3000, FIN 90014 University of Oulu, Finland
    J Mol Biol 327:47-59. 2003
    ..Our data indicate that the mitochondrial reductase activity is indispensable for respiratory function in yeast, emphasizing the significance of Mrf1p (Etr1p) and mitochondrial FAS for the integrity of the respiratory competent organelle...
  32. doi request reprint Peroxisomes are oxidative organelles
    Vasily D Antonenkov
    Department of Biochemistry, University of Oulu, Oulu, Finland
    Antioxid Redox Signal 13:525-37. 2010
    ..Antioxid...
  33. ncbi request reprint Amino acid sequence similarities of the mitochondrial short chain delta 3, delta 2-enoyl-CoA isomerase and peroxisomal multifunctional delta 3, delta 2-enoyl-CoA isomerase, 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase enzyme in rat liver. The pr
    P M Palosaari
    Department of Medical Biochemistry, University of Oulu, Finland
    J Biol Chem 266:10750-3. 1991
    ..M., and Hiltunen, J. K. (1990) J. Biol. Chem. 265, 2446-2449)...
  34. ncbi request reprint Candida tropicalis expresses two mitochondrial 2-enoyl thioester reductases that are able to form both homodimers and heterodimers
    Juha M Torkko
    Biocenter Oulu, Department of Biochemistry, University of Oulu, Finland
    J Biol Chem 278:41213-20. 2003
    ..Resolving of crystal structures obtained via Etr2p and Etr1p co-crystallization indicated that all possible dimer variants occur in the same asymmetric unit, suggesting that similar dimer formation also takes place in vivo...
  35. doi request reprint Structural enzymological studies of 2-enoyl thioester reductase of the human mitochondrial FAS II pathway: new insights into its substrate recognition properties
    Zhi jun Chen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O Box 3000, FI 90014, Oulu, Finland
    J Mol Biol 379:830-44. 2008
    ..Furthermore, the kinetic analysis of the wild-type MECR/ETR1 shows a bimodal distribution of catalytic efficiencies, in agreement with the notion that two major products are generated by the mitochondrial FAS II pathway...
  36. ncbi request reprint Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2
    Kristian M Koski
    Department of Biochemistry and Biocenter Oulu, University of Oulu, Box 3000, FIN 90014 Oulu, Finland
    J Mol Biol 345:1157-69. 2005
    ..Since hydratase 2 is the last functional unit of mammalian MFE-2 whose structure has been solved, the organization of the functional units in the biologically active full-length enzyme is also discussed...
  37. ncbi request reprint A two-domain structure of one subunit explains unique features of eukaryotic hydratase 2
    M Kristian Koski
    Department of Biochemistry and Biocenter Oulu, University of Oulu, P O Box 3000, FIN 90014 University of Oulu, Finland
    J Biol Chem 279:24666-72. 2004
    ..This arrangement allows the reaction to occur by similar mechanism, supported by mutagenesis and mechanistic studies, although via reciprocal stereochemistry...
  38. ncbi request reprint Solute traffic across mammalian peroxisomal membrane--single channel conductance monitoring reveals pore-forming activities in peroxisomes
    V D Antonenkov
    Department of Biochemistry, Biocenter Oulu, Oulu University, Linnanmaa, P O Box 3000, Oulu, 90014, Finland
    Cell Mol Life Sci 62:2886-95. 2005
    ..The peroxisomal channels were well preserved in membrane preparations but became unstable after solubilization from the membranes by detergent...
  39. pmc Pxmp2 is a channel-forming protein in Mammalian peroxisomal membrane
    Aare Rokka
    Department of Biochemistry, Biocenter Oulu, University of Oulu, Oulu, Finland
    PLoS ONE 4:e5090. 2009
    ..Peroxisomal metabolic machinery requires a continuous flow of organic and inorganic solutes across peroxisomal membrane. Concerning small solutes, the molecular nature of their traffic has remained an enigma...
  40. ncbi request reprint Binary structure of the two-domain (3R)-hydroxyacyl-CoA dehydrogenase from rat peroxisomal multifunctional enzyme type 2 at 2.38 A resolution
    Antti M Haapalainen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O Box 3000, FIN 90014 University of Oulu, Oulu, Finland
    Structure 11:87-97. 2003
    ..Peroxisomal diseases that arise because of point mutations in the dehydrogenase-coding region of the MFE-2 gene can be mapped to changes in amino acids involved in NAD(+) binding and protein dimerization...
  41. pmc Mitochondrial 2,4-dienoyl-CoA reductase deficiency in mice results in severe hypoglycemia with stress intolerance and unimpaired ketogenesis
    Ilkka J Miinalainen
    Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland
    PLoS Genet 5:e1000543. 2009
    ..This study highlights the necessity of DECR and the breakdown of unsaturated fatty acids in the transition of intermediary metabolism from the fed to the fasted state...
  42. ncbi request reprint Structural studies of MFE-1: the 1.9 A crystal structure of the dehydrogenase part of rat peroxisomal MFE-1
    Jukka P Taskinen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O Box 3000, FIN 90014, Finland
    J Mol Biol 355:734-46. 2006
    ..It is also shown that this helix corresponds to the C-terminal helix-10 of the hydratase/isomerase superfamily, suggesting that functionally it belongs to the N-terminal hydratase part of MFE-1...
  43. ncbi request reprint Characterization of 2-enoyl thioester reductase from mammals. An ortholog of YBR026p/MRF1'p of the yeast mitochondrial fatty acid synthesis type II
    Ilkka J Miinalainen
    Biocenter Oulu, Department of Biochemistry, University of Oulu, Finland
    J Biol Chem 278:20154-61. 2003
    ....
  44. pmc Myocardial overexpression of Mecr, a gene of mitochondrial FAS II leads to cardiac dysfunction in mouse
    Zhijun Chen
    Department of Biochemistry, Biocenter Oulu, University of Oulu, Oulu, Finland
    PLoS ONE 4:e5589. 2009
    ..Furthermore, the data suggested that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy...
  45. ncbi request reprint Structural studies on delta(3)-delta(2)-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily
    Anu M Mursula
    Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014 University of, Oulu, Finland
    FEBS Lett 557:81-7. 2004
    ....
  46. ncbi request reprint A mouse model for alpha-methylacyl-CoA racemase deficiency: adjustment of bile acid synthesis and intolerance to dietary methyl-branched lipids
    Kalle Savolainen
    Department of Biochemistry, Biocenter Oulu, University of Oulu, FIN 90014, Finland
    Hum Mol Genet 13:955-65. 2004
    ..Based upon our mouse model, we propose elimination of phytol from the diet of patients suffering from Amacr deficiency...
  47. ncbi request reprint Crystal structure of yeast peroxisomal multifunctional enzyme: structural basis for substrate specificity of (3R)-hydroxyacyl-CoA dehydrogenase units
    Mari S Ylianttila
    Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014, University of Oulu, Finland
    J Mol Biol 358:1286-95. 2006
    ....
  48. ncbi request reprint Alpha-methylacyl-CoA racemase from Mycobacterium tuberculosis. Mutational and structural characterization of the active site and the fold
    Kalle Savolainen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, P O Box 3000, FIN 90014 University of Oulu, Finland
    J Biol Chem 280:12611-20. 2005
    ....
  49. ncbi request reprint The 1.3 A crystal structure of human mitochondrial Delta3-Delta2-enoyl-CoA isomerase shows a novel mode of binding for the fatty acyl group
    Sanna T Partanen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014 University of Oulu, Finland
    J Mol Biol 342:1197-208. 2004
    ....
  50. ncbi request reprint The catalysis of the 1,1-proton transfer by alpha-methyl-acyl-CoA racemase is coupled to a movement of the fatty acyl moiety over a hydrophobic, methionine-rich surface
    Prasenjit Bhaumik
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, P O Box 3000, FIN 90014 University of Oulu, Finland
    J Mol Biol 367:1145-61. 2007
    ....
  51. ncbi request reprint Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2
    M Kristian Koski
    Department of Biochemistry, University of Oulu, Box 3000, FIN 90014 University of Oulu, Finland
    Acta Crystallogr D Biol Crystallogr 59:1302-5. 2003
    ..48 degrees. Selenomethionine-labelled protein was used for a multi-wavelength anomalous dispersion (MAD) experiment. A three-wavelength data set suitable for MAD phasing was collected to 2.25 A resolution using synchrotron radiation...
  52. pmc Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity
    Tiila Riikka Kiema
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O Box 3000, FIN 90014, Finland
    Biochem J 367:433-41. 2002
    ....
  53. ncbi request reprint Crystallization and characterization of the dehydrogenase domain from rat peroxisomal multifunctional enzyme type 1
    Jukka P Taskinen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014, Finland
    Acta Crystallogr D Biol Crystallogr 58:690-3. 2002
    ..45 A resolution was collected using a rotating-anode X-ray source. The crystals have primitive tetragonal symmetry, with unit-cell parameters a = b = 125.9, c = 60.2 A...
  54. ncbi request reprint Site-directed mutagenesis to enable and improve crystallizability of Candida tropicalis (3R)-hydroxyacyl-CoA dehydrogenase
    Mari S Ylianttila
    Biocenter Oulu and Department of Biochemistry, University of Oulu, P O Box 3000, FIN 90014 University of Oulu, Finland
    Biochem Biophys Res Commun 324:25-30. 2004
    ..The crystals diffract to a resolution of 2.2A at a conventional X-ray source...
  55. pmc Modification of the monomer composition of polyhydroxyalkanoate synthesized in Saccharomyces cerevisiae expressing variants of the beta-oxidation-associated multifunctional enzyme
    Silvia Marchesini
    Laboratoire de Biotechnologie Végétale, Département de Biologie Moléculaire Végétale, Universite de Lausanne, CH 1015 Lausanne, Switzerland
    Appl Environ Microbiol 69:6495-9. 2003
    ....
  56. ncbi request reprint Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers
    Yong Mei Qin
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, China
    Cell Res 15:465-73. 2005
    ..Both GhKCR(s) showed NADPH-dependent 3-ketoacyl-CoA reductase activity in an in vitro assay system using palmitoyl-CoA and malonyl-CoA as substrates. Our results suggest that GhKCR1 and 2 are functional orthologues of ScYbr159p...
  57. pmc Intersection of RNA processing and the type II fatty acid synthesis pathway in yeast mitochondria
    Melissa S Schonauer
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721 0106, USA
    Mol Cell Biol 28:6646-57. 2008
    ..These two positive feedback cycles may provide switch-like control of mitochondrial gene expression in response to the metabolic state of the cell...
  58. ncbi request reprint Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana
    Simon Goepfert
    Département de Biologie Moléculaire Végétale, Biophore Building, Universite de Lausanne, CH 1015, Switzerland
    J Biol Chem 281:35894-903. 2006
    ....
  59. ncbi request reprint Peroxisomal beta-oxidation--a metabolic pathway with multiple functions
    Yves Poirier
    Department of Plant Molecular Biology, Biophore, University of Lausanne, CH 1015 Lausanne, Switzerland
    Biochim Biophys Acta 1763:1413-26. 2006
    ....
  60. pmc Mutational spectrum of D-bifunctional protein deficiency and structure-based genotype-phenotype analysis
    Sacha Ferdinandusse
    Laboratory Genetic Metabolic Diseases, Academic Medical Center at University of Amsterdam, Amsterdam, The Netherlands
    Am J Hum Genet 78:112-24. 2006
    ..From our data, we conclude that, on the basis of the predicted effect of the mutations on protein structure, a genotype-phenotype correlation exists for DBP deficiency...
  61. ncbi request reprint Genetic and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongation
    Yong Mei Qin
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China
    J Exp Bot 58:473-81. 2007
    ..The results suggest that GhCER6 encodes a functional 3-ketoacyl-CoA synthase...
  62. pmc Enhanced polyamine catabolism alters homeostatic control of white adipose tissue mass, energy expenditure, and glucose metabolism
    Eija Pirinen
    Department of Medicine, University of Kuopio, P O Box 1777, FI 70211 Kuopio, Finland
    Mol Cell Biol 27:4953-67. 2007
    ..The involvement of polyamine catabolism in the regulation of energy and glucose metabolism may offer a novel target for drug development for obesity and type 2 diabetes...
  63. pmc Molecular identification and characterization of the Arabidopsis delta(3,5),delta(2,4)-dienoyl-coenzyme A isomerase, a peroxisomal enzyme participating in the beta-oxidation cycle of unsaturated fatty acids
    Simon Goepfert
    Département de Biologie Moléculaire Végétale, Batiment de Biologie, Universite de Lausanne, CH 1015 Lausanne, Switzerland
    Plant Physiol 138:1947-56. 2005
    ..Proteins showing high homology with AtDCI1 are found in gymnosperms as well as angiosperms belonging to the Monocotyledon or Dicotyledon classes...
  64. pmc Identification of a novel mycobacterial 3-hydroxyacyl-thioester dehydratase, HtdZ (Rv0130), by functional complementation in yeast
    Aner Gurvitz
    Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    J Bacteriol 190:4088-90. 2008
    ..Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis...
  65. pmc Function of heterologous Mycobacterium tuberculosis InhA, a type 2 fatty acid synthase enzyme involved in extending C20 fatty acids to C60-to-C90 mycolic acids, during de novo lipoic acid synthesis in Saccharomyces cerevisiae
    Aner Gurvitz
    Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Appl Environ Microbiol 74:5078-85. 2008
    ..tuberculosis and the potential use of yeast FASII mutants for investigating the physiological function of drug-targeted pathogen enzymes involved in fatty acid biosynthesis...
  66. pmc Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis
    Yong Mei Qin
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Plant Cell 19:3692-704. 2007
    ..Thus, VLCFAs may be involved in maximizing the extensibility of cotton fibers and multiple Arabidopsis cell types, possibly by activating ethylene biosynthesis...
  67. ncbi request reprint Crystallization and preliminary X-ray diffraction studies of an alpha-methylacyl-CoA racemase from Mycobacterium tuberculosis
    Prasenjit Bhaumik
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, Box 3000, FIN 90014 Oulu, Finland
    Acta Crystallogr D Biol Crystallogr 59:353-5. 2003
    ..The crystals of the unliganded racemase belong to space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 122.0, c = 256.4 A. Data sets were collected at 100 K. The crystals diffract to 2.8 A using synchrotron radiation...