Research Topics
| Harri HarmaSummaryAffiliation: University of Turku Country: Finland Publications
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Detail Information
Publications
Protein quantification using resonance energy transfer between donor nanoparticles and acceptor quantum dotsHarri Harma
Laboratory of Biophysics, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
Anal Chem 85:2921-6. 2013..The assay sensitivity is improved, when compared to the most sensitive commercial methods. The presented mix-and-measure method has potential to be implemented into routine protein quantification in biological laboratories...
Time-resolved fluorescence immunoassay for C-reactive protein using colloidal semiconducting nanoparticlesHarri Harma
Laboratory of Biophysics, University of Turku, 20520 Turku, Finland
Sensors (Basel) 11:11335-42. 2011..The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation...- Rapid detection of trace amounts of surfactants using nanoparticles in fluorometric assaysHarri Harma
Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, Turku, Finland
Nanoscale 2:69-71. 2010.... - Comparison of homogeneous single-label fluorometric binding assays: fluorescence polarization and dual-parametric quenching resonance energy transfer techniqueHarri Harma
Laboratory of Biophysics, University of Turku, FI 20520 Turku, Finland
Anal Chem 82:892-7. 2010..Therefore, multiplexing of the QRET assay was investigated and its feasibility was successfully demonstrated in a dual-parametric assay using estradiol labeled with europium(III) and terbium(III) chelates... - A new simple cell-based homogeneous time-resolved fluorescence QRET technique for receptor-ligand interaction screeningHarri Harma
Laboratory of Biophysics, University of Turku, Turku, Finland
J Biomol Screen 14:936-43. 2009..This simple and cost-effective technique can be readily adapted to laboratory and industrial-scale screening... - Sensitive quantitative protein concentration method using luminescent resonance energy transfer on a layer-by-layer europium(III) chelate particle sensorHarri Harma
Laboratory of Biophysics and Medicity, University of Turku, FIN 20520 Turku, Finland
Anal Chem 80:9781-6. 2008..Compared to known protein concentration methods, the developed method required no hazardous substances or elevated temperature to reach the high-sensitivity level... 
Luminescent energy transfer between cadmium telluride nanoparticle and lanthanide(III) chelate in competitive bioaffinity assays of biotin and estradiolHarri Harma
Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
Anal Chim Acta 604:177-83. 2007..The data suggested that relatively short distance was needed to obtain adequate energy transfer. Therefore, biomolecules were coupled onto the semiconductor nanoparticles without any spacers...- Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragmentsH Harma
Department of Biotechnology, University of Turku, Tykistokatu 6A, FIN 20520 Turku, Finland
Clin Chem 46:1755-61. 2000..We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum in a submicroliter volume single-particle immunoassay... - Europium nanoparticles and time-resolved fluorescence for ultrasensitive detection of prostate-specific antigenH Harma
Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
Clin Chem 47:561-8. 2001.... - Sensitive method for determination of protein and cell concentrations based on competitive adsorption to nanoparticles and time-resolved luminescence resonance energy transfer between labeled proteinsSari Pihlasalo
Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
Anal Chem 84:4950-6. 2012..The developed method requires no labeled particles, making the concept optimally applicable to varying targets as the material of the particle may be selected according to the application... - Preparation, characterisation and application of europium(III) chelate-dyed polystyrene-acrylic acid nanoparticle labelsPetri Huhtinen
Department of Biotechnology, University of Turku, Turku, Finland
Anal Chim Acta 630:211-6. 2008.... - Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technologyPetri Huhtinen
Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
J Immunol Methods 294:111-22. 2004..The general characteristics of this combination should permit the development of various immunoassay applications featuring high sensitivity, rapidity, and low consumption of reagents... - Study on nonspecificity of an immuoassay using Eu-doped polystyrene nanoparticle labelsTuomas Näreoja
Laboratory of Biophysics, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
J Immunol Methods 345:80-9. 2009..This study indicated that nonspecific binding is not readily affected by the physical size of the nanoparticle label or antibodies used in the assay... - Multiple sized europium(III) chelate-dyed polystyrene particles as donors in FRET - an application for sensitive protein quantification utilizing competitive adsorptionAntti Valanne
University of Turku, Department of Biotechnology, Tykistokatu 6A, FIN 20520 Turku, Finland
Analyst 134:980-6. 2009.... - Rapid and sensitive HBsAg immunoassay based on fluorescent nanoparticle labels and time-resolved detectionAntti Valanne
University of Turku, Department of Biotechnology, Tykistokatu 6, 6th Floor, 20520 Turku, Finland
J Virol Methods 129:83-90. 2005..8-21.9%). Furthermore, concurrent sample runs with the ELISA reference method showed 100% agreement. The time required for the assay was only 10 min facilitating a rapid and convenient method for hepatitis B screening... - Sensitive luminometric method for protein quantification in bacterial cell lysate based on particle adsorption and dissociation of chelated europiumSari Pihlasalo
Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
Anal Chem 84:1386-93. 2012..The developed method quantified protein also in samples containing insoluble cell components reducing the need for additional centrifugal assay steps and making the concept highly attractive for routine laboratory work... - Single-label time-resolved luminescence assay for estrogen receptor--ligand bindingRoope Huttunen
Laboratory of Biophysics, Department of Cell Biology and Anatomy and Medicity Research Laboratories, Institute of Biomedicine, University of Turku, 20520 Turku, Finland
Anal Biochem 415:27-31. 2011..3-1.5). A K(d) value of 30nM was calculated for binding of Eu-E(2) to ER from a saturation binding isotherm. The QRET method provides an attractive new single-label assay format for nuclear receptor ligand screening... - Sensitive fluorometric nanoparticle assays for cell counting and viabilitySari Pihlasalo
Laboratory of Biophysics and Medicity, University of Turku, Tykistökatu 6A, FI 20520 Turku, Finland
Anal Chem 82:9282-8. 2010..This sensitive and easy-to-use nanoparticle sensor combined with a viability test for a low concentration of cells could potentially replace existing microscopic methods in biochemical laboratories... - Impact of surface defects and denaturation of capture surface proteins on nonspecific binding in immunoassays using antibody-coated polystyrene nanoparticle labelsTuomas Näreoja
Laboratory of Biophysics, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
J Immunol Methods 347:24-30. 2009..Evidently, the additional protein (SA) layer protected the capture antibody from denaturation whereas the solid surface appeared to act as a "catalyst" making the passively adsorbed antibody more susceptible to denaturation... - Cell-based beta2-adrenergic receptor-ligand binding assay using synthesized europium-labeled ligands and time-resolved fluorescenceEija Martikkala
Laboratory of Biophysics, University of Turku, FI 20520 Turku, Finland
Anal Biochem 392:103-9. 2009..5 and 16.0 for C0 and C12, respectively. This study shows that functional time-resolved fluorescent assays can be constructed using fluorescent lanthanide chelates conjugated to small-molecule ligands... - Method for estimation of protein isoelectric pointSari Pihlasalo
Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
Anal Chem 84:8253-8. 2012..This mix-and-measure concept is a highly attractive alternative for routine laboratory work... - Liposome-based homogeneous luminescence resonance energy transferSari Pihlasalo
Laboratory of Biophysics, University of Turku, FIN 20520 Turku, Finland
Anal Biochem 384:231-7. 2009..The developed lipid membrane-based system was feasible in separation free LRET assay concept with high sensitivity, indicating that the assay principle can potentially be used for biologically more relevant target molecules... - Homogeneous GTP binding assay employing QRET technologyAnita Rozwandowicz-Jansen
Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland
J Biomol Screen 15:261-7. 2010.... - A sensitive adenovirus immunoassay as a model for using nanoparticle label technology in virus diagnosticsAntti Valanne
Department of Biotechnology, University of Turku, Tykistokatu 6, 20520 Turku, Finland
J Clin Virol 33:217-23. 2005..Methods relying on viral antigen detection, e.g. immunofluorometric assays (IFMAs) and enzyme immunoassays (EIAs), are less complicated to carry out, but they provide limited sensitivity... - Ultrasensitive protein concentration measurement based on particle adsorption and fluorescence quenchingSari Pihlasalo
Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
Anal Chem 81:4995-5000. 2009..Eleven different proteins were tested with the constructed sensor exhibiting a protein-to-protein variability less than 15% allowing protein concentration measurements without the need for recalibration of different proteins... - A homogeneous single-label time-resolved fluorescence cAMP assayEija Martikkala
Laboratory of Biophysics and Medicity, University of Turku, Turku, Finland
J Biomol Screen 16:356-62. 2011.... - Homogeneous single-label biochemical ras activation assay using time-resolved luminescenceEija Martikkala
Laboratory of Biophysics and Medicity, University of Turku, FI 20520 Turku, Finland
Anal Chem 83:9230-3. 2011..The need of only one labeled molecule and the ability to decrease the quantities of purified proteins used in the experiments are valuable qualities in HTS showing the potential of the QRET method... - Highly sensitive immunoassay of free prostate-specific antigen in serum using europium(III) nanoparticle label technologyTero Soukka
Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
Clin Chim Acta 328:45-58. 2003..Our objective was to evaluate the performance of the nanoparticle label technology with biological samples in an immunoassay of free prostate-specific antigen (PSA-F) using a standard microtitration well platform... - Multiparametric homogeneous method for identification of ligand binding to G protein-coupled receptors: receptor-ligand binding and β-arrestin assayKari Kopra
Laboratory of Biophysics, Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland
Anal Chem 85:2276-81. 2013..Combined homogeneous assay technique can provide an informative method for screening purposes and an efficient way to monitor receptor-ligand interaction, thus separating agonist from antagonist... - Nonspecific particle-based method with two-photon excitation detection for sensitive protein quantification and cell countingSari Pihlasalo
Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
Anal Chem 85:2689-96. 2013..The developed method tolerates interfering agents such as neutral detergents found in cell lysate samples even at high concentrations. The method is experimentally fairly simple and allows the expansion for the use of the TPX technology... - Multiparametric luminescence method for quantitative cell surface protein expression analysis and imagingRoope Huttunen
Laboratory of Biophysics, Department of Cell Biology and Anatomy and Medicity Research Laboratories, Institute of Biomedicine, University of Turku, Turku, Finland
J Immunol Methods 367:40-6. 2011..The novel approach proposed in this paper can be potentially applied to cell surface protein expression analysis in screening applications combined with localization studies of the target proteins by fluorescence microscopy imaging... - Synthesis and characterization of europium(III) nanoparticles for time-resolved fluoroimmunoassay of prostate-specific antigenHarri Harma
Laboratory of Biophysics, University of Turku, Tykistokatu 6A, Turku 20520, Finland
Nanotechnology 18:075604. 2007..In addition, prepared high-density nanoparticle labels using lanthanide(III) chelates or other specific fluorochromes have potential applications in a number of other fields...