Harri Harma

Summary

Affiliation: University of Turku
Country: Finland

Publications

  1. doi request reprint Protein quantification using resonance energy transfer between donor nanoparticles and acceptor quantum dots
    Harri Harma
    Laboratory of Biophysics, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chem 85:2921-6. 2013
  2. pmc Time-resolved fluorescence immunoassay for C-reactive protein using colloidal semiconducting nanoparticles
    Harri Harma
    Laboratory of Biophysics, University of Turku, 20520 Turku, Finland
    Sensors (Basel) 11:11335-42. 2011
  3. doi request reprint A new simple cell-based homogeneous time-resolved fluorescence QRET technique for receptor-ligand interaction screening
    Harri Harma
    Laboratory of Biophysics, University of Turku, Turku, Finland
    J Biomol Screen 14:936-43. 2009
  4. doi request reprint Rapid detection of trace amounts of surfactants using nanoparticles in fluorometric assays
    Harri Harma
    Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, Turku, Finland
    Nanoscale 2:69-71. 2010
  5. doi request reprint Sensitive quantitative protein concentration method using luminescent resonance energy transfer on a layer-by-layer europium(III) chelate particle sensor
    Harri Harma
    Laboratory of Biophysics and Medicity, University of Turku, FIN 20520 Turku, Finland
    Anal Chem 80:9781-6. 2008
  6. ncbi request reprint Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments
    H Harma
    Department of Biotechnology, University of Turku, Tykistokatu 6A, FIN 20520 Turku, Finland
    Clin Chem 46:1755-61. 2000
  7. ncbi request reprint Luminescent energy transfer between cadmium telluride nanoparticle and lanthanide(III) chelate in competitive bioaffinity assays of biotin and estradiol
    Harri Harma
    Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chim Acta 604:177-83. 2007
  8. doi request reprint Comparison of homogeneous single-label fluorometric binding assays: fluorescence polarization and dual-parametric quenching resonance energy transfer technique
    Harri Harma
    Laboratory of Biophysics, University of Turku, FI 20520 Turku, Finland
    Anal Chem 82:892-7. 2010
  9. ncbi request reprint Europium nanoparticles and time-resolved fluorescence for ultrasensitive detection of prostate-specific antigen
    H Harma
    Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
    Clin Chem 47:561-8. 2001
  10. ncbi request reprint Synthesis, characterization, and application of Eu(III), Tb(III), Sm(III), and Dy(III) lanthanide chelate nanoparticle labels
    Petri Huhtinen
    Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
    Anal Chem 77:2643-8. 2005

Collaborators

Detail Information

Publications38

  1. doi request reprint Protein quantification using resonance energy transfer between donor nanoparticles and acceptor quantum dots
    Harri Harma
    Laboratory of Biophysics, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chem 85:2921-6. 2013
    ..The assay sensitivity is improved, when compared to the most sensitive commercial methods. The presented mix-and-measure method has potential to be implemented into routine protein quantification in biological laboratories...
  2. pmc Time-resolved fluorescence immunoassay for C-reactive protein using colloidal semiconducting nanoparticles
    Harri Harma
    Laboratory of Biophysics, University of Turku, 20520 Turku, Finland
    Sensors (Basel) 11:11335-42. 2011
    ..The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation...
  3. doi request reprint A new simple cell-based homogeneous time-resolved fluorescence QRET technique for receptor-ligand interaction screening
    Harri Harma
    Laboratory of Biophysics, University of Turku, Turku, Finland
    J Biomol Screen 14:936-43. 2009
    ..This simple and cost-effective technique can be readily adapted to laboratory and industrial-scale screening...
  4. doi request reprint Rapid detection of trace amounts of surfactants using nanoparticles in fluorometric assays
    Harri Harma
    Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, Turku, Finland
    Nanoscale 2:69-71. 2010
    ....
  5. doi request reprint Sensitive quantitative protein concentration method using luminescent resonance energy transfer on a layer-by-layer europium(III) chelate particle sensor
    Harri Harma
    Laboratory of Biophysics and Medicity, University of Turku, FIN 20520 Turku, Finland
    Anal Chem 80:9781-6. 2008
    ..Compared to known protein concentration methods, the developed method required no hazardous substances or elevated temperature to reach the high-sensitivity level...
  6. ncbi request reprint Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments
    H Harma
    Department of Biotechnology, University of Turku, Tykistokatu 6A, FIN 20520 Turku, Finland
    Clin Chem 46:1755-61. 2000
    ..We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum in a submicroliter volume single-particle immunoassay...
  7. ncbi request reprint Luminescent energy transfer between cadmium telluride nanoparticle and lanthanide(III) chelate in competitive bioaffinity assays of biotin and estradiol
    Harri Harma
    Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chim Acta 604:177-83. 2007
    ..The data suggested that relatively short distance was needed to obtain adequate energy transfer. Therefore, biomolecules were coupled onto the semiconductor nanoparticles without any spacers...
  8. doi request reprint Comparison of homogeneous single-label fluorometric binding assays: fluorescence polarization and dual-parametric quenching resonance energy transfer technique
    Harri Harma
    Laboratory of Biophysics, University of Turku, FI 20520 Turku, Finland
    Anal Chem 82:892-7. 2010
    ..Therefore, multiplexing of the QRET assay was investigated and its feasibility was successfully demonstrated in a dual-parametric assay using estradiol labeled with europium(III) and terbium(III) chelates...
  9. ncbi request reprint Europium nanoparticles and time-resolved fluorescence for ultrasensitive detection of prostate-specific antigen
    H Harma
    Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
    Clin Chem 47:561-8. 2001
    ....
  10. ncbi request reprint Synthesis, characterization, and application of Eu(III), Tb(III), Sm(III), and Dy(III) lanthanide chelate nanoparticle labels
    Petri Huhtinen
    Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
    Anal Chem 77:2643-8. 2005
    ..The spectral and functional properties of the lanthanide-embedded polystyrene nanoparticles developed here suggest that the technology is applicable for high-sensitivity multicolor assays...
  11. doi request reprint Sensitive method for determination of protein and cell concentrations based on competitive adsorption to nanoparticles and time-resolved luminescence resonance energy transfer between labeled proteins
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
    Anal Chem 84:4950-6. 2012
    ..The developed method requires no labeled particles, making the concept optimally applicable to varying targets as the material of the particle may be selected according to the application...
  12. doi request reprint Preparation, characterisation and application of europium(III) chelate-dyed polystyrene-acrylic acid nanoparticle labels
    Petri Huhtinen
    Department of Biotechnology, University of Turku, Turku, Finland
    Anal Chim Acta 630:211-6. 2008
    ....
  13. doi request reprint Luminescence stability improvement in liposome-based homogeneous luminescence resonance energy transfer
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, 20520 Turku, Finland
    Anal Biochem 440:6-8. 2013
    ..Potentially, the stable assay compared with the assay without cholesterol offers an improved platform to liposome-based detection systems...
  14. ncbi request reprint Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology
    Petri Huhtinen
    Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
    J Immunol Methods 294:111-22. 2004
    ..The general characteristics of this combination should permit the development of various immunoassay applications featuring high sensitivity, rapidity, and low consumption of reagents...
  15. doi request reprint Multiple sized europium(III) chelate-dyed polystyrene particles as donors in FRET - an application for sensitive protein quantification utilizing competitive adsorption
    Antti Valanne
    University of Turku, Department of Biotechnology, Tykistokatu 6A, FIN 20520 Turku, Finland
    Analyst 134:980-6. 2009
    ....
  16. doi request reprint Study on nonspecificity of an immuoassay using Eu-doped polystyrene nanoparticle labels
    Tuomas Näreoja
    Laboratory of Biophysics, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    J Immunol Methods 345:80-9. 2009
    ..This study indicated that nonspecific binding is not readily affected by the physical size of the nanoparticle label or antibodies used in the assay...
  17. ncbi request reprint Rapid and sensitive HBsAg immunoassay based on fluorescent nanoparticle labels and time-resolved detection
    Antti Valanne
    University of Turku, Department of Biotechnology, Tykistokatu 6, 6th Floor, 20520 Turku, Finland
    J Virol Methods 129:83-90. 2005
    ..8-21.9%). Furthermore, concurrent sample runs with the ELISA reference method showed 100% agreement. The time required for the assay was only 10 min facilitating a rapid and convenient method for hepatitis B screening...
  18. doi request reprint Nonspecific particle-based method with two-photon excitation detection for sensitive protein quantification and cell counting
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chem 85:2689-96. 2013
    ..The developed method tolerates interfering agents such as neutral detergents found in cell lysate samples even at high concentrations. The method is experimentally fairly simple and allows the expansion for the use of the TPX technology...
  19. ncbi request reprint Quantitative, rapid europium(III) nanoparticle-label-based all-in-one dry-reagent immunoassay for thyroid-stimulating hormone
    Petri Huhtinen
    Department of Biotechnology, University of Turku, Tykistokatu 6A, Turku FIN 20520, Finland
    Clin Chem 50:1935-6. 2004
  20. doi request reprint Sensitive luminometric method for protein quantification in bacterial cell lysate based on particle adsorption and dissociation of chelated europium
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
    Anal Chem 84:1386-93. 2012
    ..The developed method quantified protein also in samples containing insoluble cell components reducing the need for additional centrifugal assay steps and making the concept highly attractive for routine laboratory work...
  21. doi request reprint Impact of surface defects and denaturation of capture surface proteins on nonspecific binding in immunoassays using antibody-coated polystyrene nanoparticle labels
    Tuomas Näreoja
    Laboratory of Biophysics, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    J Immunol Methods 347:24-30. 2009
    ..Evidently, the additional protein (SA) layer protected the capture antibody from denaturation whereas the solid surface appeared to act as a "catalyst" making the passively adsorbed antibody more susceptible to denaturation...
  22. doi request reprint Sensitive fluorometric nanoparticle assays for cell counting and viability
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity, University of Turku, Tykistökatu 6A, FI 20520 Turku, Finland
    Anal Chem 82:9282-8. 2010
    ..This sensitive and easy-to-use nanoparticle sensor combined with a viability test for a low concentration of cells could potentially replace existing microscopic methods in biochemical laboratories...
  23. doi request reprint Single-label time-resolved luminescence assay for estrogen receptor--ligand binding
    Roope Huttunen
    Laboratory of Biophysics, Department of Cell Biology and Anatomy and Medicity Research Laboratories, Institute of Biomedicine, University of Turku, 20520 Turku, Finland
    Anal Biochem 415:27-31. 2011
    ..3-1.5). A K(d) value of 30nM was calculated for binding of Eu-E(2) to ER from a saturation binding isotherm. The QRET method provides an attractive new single-label assay format for nuclear receptor ligand screening...
  24. doi request reprint Multiparametric homogeneous method for identification of ligand binding to G protein-coupled receptors: receptor-ligand binding and β-arrestin assay
    Kari Kopra
    Laboratory of Biophysics, Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland
    Anal Chem 85:2276-81. 2013
    ..Combined homogeneous assay technique can provide an informative method for screening purposes and an efficient way to monitor receptor-ligand interaction, thus separating agonist from antagonist...
  25. doi request reprint Liposome-based homogeneous luminescence resonance energy transfer
    Sari Pihlasalo
    Laboratory of Biophysics, University of Turku, FIN 20520 Turku, Finland
    Anal Biochem 384:231-7. 2009
    ..The developed lipid membrane-based system was feasible in separation free LRET assay concept with high sensitivity, indicating that the assay principle can potentially be used for biologically more relevant target molecules...
  26. doi request reprint Cell-based beta2-adrenergic receptor-ligand binding assay using synthesized europium-labeled ligands and time-resolved fluorescence
    Eija Martikkala
    Laboratory of Biophysics, University of Turku, FI 20520 Turku, Finland
    Anal Biochem 392:103-9. 2009
    ..5 and 16.0 for C0 and C12, respectively. This study shows that functional time-resolved fluorescent assays can be constructed using fluorescent lanthanide chelates conjugated to small-molecule ligands...
  27. doi request reprint Ultrasensitive protein concentration measurement based on particle adsorption and fluorescence quenching
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chem 81:4995-5000. 2009
    ..Eleven different proteins were tested with the constructed sensor exhibiting a protein-to-protein variability less than 15% allowing protein concentration measurements without the need for recalibration of different proteins...
  28. doi request reprint Method for estimation of protein isoelectric point
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, FI 20520 Turku, Finland
    Anal Chem 84:8253-8. 2012
    ..This mix-and-measure concept is a highly attractive alternative for routine laboratory work...
  29. doi request reprint Homogeneous GTP binding assay employing QRET technology
    Anita Rozwandowicz-Jansen
    Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland
    J Biomol Screen 15:261-7. 2010
    ....
  30. ncbi request reprint A sensitive adenovirus immunoassay as a model for using nanoparticle label technology in virus diagnostics
    Antti Valanne
    Department of Biotechnology, University of Turku, Tykistokatu 6, 20520 Turku, Finland
    J Clin Virol 33:217-23. 2005
    ..Methods relying on viral antigen detection, e.g. immunofluorometric assays (IFMAs) and enzyme immunoassays (EIAs), are less complicated to carry out, but they provide limited sensitivity...
  31. doi request reprint A homogeneous single-label time-resolved fluorescence cAMP assay
    Eija Martikkala
    Laboratory of Biophysics and Medicity, University of Turku, Turku, Finland
    J Biomol Screen 16:356-62. 2011
    ....
  32. doi request reprint A homogeneous single-label quenching resonance energy transfer assay for a δ-opioid receptor-ligand using intact cells
    Kari Kopra
    Laboratory of Biophysics, Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Tykistökatu 6 A 5th floor, FI 20520 Turku, Finland
    Analyst 138:4907-14. 2013
    ..0. The QRET method showed prominent performance also in high DMSO concentrations. QRET is a homogenous and a non-radioactive detection system for screening and this is the first attempt to utilize peptide ligands in the QRET concept...
  33. ncbi request reprint Homogeneous single-label biochemical ras activation assay using time-resolved luminescence
    Eija Martikkala
    Laboratory of Biophysics and Medicity, University of Turku, FI 20520 Turku, Finland
    Anal Chem 83:9230-3. 2011
    ..The need of only one labeled molecule and the ability to decrease the quantities of purified proteins used in the experiments are valuable qualities in HTS showing the potential of the QRET method...
  34. ncbi request reprint Highly sensitive immunoassay of free prostate-specific antigen in serum using europium(III) nanoparticle label technology
    Tero Soukka
    Department of Biotechnology, University of Turku, Tykistokatu 6, FIN 20520 Turku, Finland
    Clin Chim Acta 328:45-58. 2003
    ..Our objective was to evaluate the performance of the nanoparticle label technology with biological samples in an immunoassay of free prostate-specific antigen (PSA-F) using a standard microtitration well platform...
  35. doi request reprint Multiparametric luminescence method for quantitative cell surface protein expression analysis and imaging
    Roope Huttunen
    Laboratory of Biophysics, Department of Cell Biology and Anatomy and Medicity Research Laboratories, Institute of Biomedicine, University of Turku, Turku, Finland
    J Immunol Methods 367:40-6. 2011
    ..The novel approach proposed in this paper can be potentially applied to cell surface protein expression analysis in screening applications combined with localization studies of the target proteins by fluorescence microscopy imaging...
  36. doi request reprint Simple nanoparticle-based luminometric method for molecular weight determination of polymeric compounds
    Sari Pihlasalo
    Laboratory of Biophysics and Medicity Research Laboratory, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    Anal Chem 86:1038-44. 2014
    ....
  37. doi request reprint Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay
    Tuomas Näreoja
    Laboratory of Biophysics, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Tykistokatu 6A, 20520, Turku, Finland
    Anal Bioanal Chem 406:493-503. 2014
    ..Most importantly, nonspecific binding of a nanoparticle was reduced at contact times below 100 ms with the solid-phase surface. ..
  38. ncbi request reprint Synthesis and characterization of europium(III) nanoparticles for time-resolved fluoroimmunoassay of prostate-specific antigen
    Harri Harma
    Laboratory of Biophysics, University of Turku, Tykistokatu 6A, Turku 20520, Finland
    Nanotechnology 18:075604. 2007
    ..In addition, prepared high-density nanoparticle labels using lanthanide(III) chelates or other specific fluorochromes have potential applications in a number of other fields...