Eeva Liisa Eskelinen

Summary

Affiliation: University of Helsinki
Country: Finland

Publications

  1. ncbi request reprint Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, Viikinkaari 5D, 00014 Helsinki, Finland
    Mol Aspects Med 27:495-502. 2006
  2. ncbi request reprint Seeing is believing: the impact of electron microscopy on autophagy research
    Eeva Liisa Eskelinen
    Department of Biosciences, University of Helsinki, Helsinki, Finland
    Autophagy 7:935-56. 2011
  3. doi request reprint The dual role of autophagy in cancer
    Eeva Liisa Eskelinen
    University of Helsinki, Department of Biosciences, Helsinki, Finland
    Curr Opin Pharmacol 11:294-300. 2011
  4. doi request reprint Autophagy: a lysosomal degradation pathway with a central role in health and disease
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, PO Box 56, FIN 00014 Helsinki, Finland
    Biochim Biophys Acta 1793:664-73. 2009
  5. ncbi request reprint To be or not to be? Examples of incorrect identification of autophagic compartments in conventional transmission electron microscopy of mammalian cells
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
    Autophagy 4:257-60. 2008
  6. ncbi request reprint 4th International Symposium on Autophagy: exploiting the frontiers of autophagy research
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, Helsinki, Finland
    Autophagy 3:166-73. 2007
  7. ncbi request reprint Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2
    Eeva Liisa Eskelinen
    University of Kiel, Institute of Biochemistry, Kiel, Germany
    Traffic 6:1058-61. 2005
  8. pmc Disturbed cholesterol traffic but normal proteolytic function in LAMP-1/LAMP-2 double-deficient fibroblasts
    Eeva Liisa Eskelinen
    Institute of Biochemistry, University of Kiel, D 24098 Kiel, Germany
    Mol Biol Cell 15:3132-45. 2004
  9. doi request reprint Role for LAMP-2 in endosomal cholesterol transport
    Alexander Schneede
    Institute of Biochemistry, University of Kiel, Kiel, Germany
    J Cell Mol Med 15:280-95. 2011
  10. ncbi request reprint Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, Viikinkaari 5D, 00014 Helsinki, Finland
    Mol Aspects Med 27:495-502. 2006
    ..These results clearly show that the LAMP proteins fulfil functions far beyond the initially suggested roles in maintaining the structural integrity of the lysosomal compartment...
  2. ncbi request reprint Seeing is believing: the impact of electron microscopy on autophagy research
    Eeva Liisa Eskelinen
    Department of Biosciences, University of Helsinki, Helsinki, Finland
    Autophagy 7:935-56. 2011
    ..Astonishingly, some of the "novel" concepts and principles of autophagy, presented in the recent studies, were already proposed several decades ago by the pioneering, accurate and passionate work of virtuoso electron microscopists...
  3. doi request reprint The dual role of autophagy in cancer
    Eeva Liisa Eskelinen
    University of Helsinki, Department of Biosciences, Helsinki, Finland
    Curr Opin Pharmacol 11:294-300. 2011
    ..Interestingly, the FK506-binding protein 51 plays a role in the autophagy-linked radiation resistance of malignant melanoma...
  4. doi request reprint Autophagy: a lysosomal degradation pathway with a central role in health and disease
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, PO Box 56, FIN 00014 Helsinki, Finland
    Biochim Biophys Acta 1793:664-73. 2009
    ..In addition, impaired autophagic degradation contributes to the pathogenesis of several human diseases including lysosomal storage disorders and muscle diseases...
  5. ncbi request reprint To be or not to be? Examples of incorrect identification of autophagic compartments in conventional transmission electron microscopy of mammalian cells
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
    Autophagy 4:257-60. 2008
    ..In this commentary I present some common mistakes in identification of autophagic compartments in conventional transmission electron microscopy of mammalian cells...
  6. ncbi request reprint 4th International Symposium on Autophagy: exploiting the frontiers of autophagy research
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, Helsinki, Finland
    Autophagy 3:166-73. 2007
    ..Two themes were discussed in many sessions during the symposium: the role of autophagy in the degradation of aggregate-prone proteins and protein aggregates, and the possible role of p62 in autophagy...
  7. ncbi request reprint Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2
    Eeva Liisa Eskelinen
    University of Kiel, Institute of Biochemistry, Kiel, Germany
    Traffic 6:1058-61. 2005
    ..To avoid confusion in the literature, we further propose to adopt the use of capital letters for the updated nomenclature of all the isoforms in all three species: LAMP-2A, LAMP-2B, and LAMP-2C...
  8. pmc Disturbed cholesterol traffic but normal proteolytic function in LAMP-1/LAMP-2 double-deficient fibroblasts
    Eeva Liisa Eskelinen
    Institute of Biochemistry, University of Kiel, D 24098 Kiel, Germany
    Mol Biol Cell 15:3132-45. 2004
    ..Taken together these findings indicate partially overlapping functions for LAMP-1 and -2 in lysosome biogenesis, autophagy, and cholesterol homeostasis...
  9. doi request reprint Role for LAMP-2 in endosomal cholesterol transport
    Alexander Schneede
    Institute of Biochemistry, University of Kiel, Kiel, Germany
    J Cell Mol Med 15:280-95. 2011
    ..Taken together, our results suggest that LAMP-2, its luminal domain in particular, plays a critical role in endosomal cholesterol transport and that this is distinct from the chaperone-mediated autophagy function of LAMP-2...
  10. ncbi request reprint Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
    ..In conclusion, our results showed a role for Rab7 in the final maturation of late autophagic vacuoles...
  11. ncbi request reprint At the acidic edge: emerging functions for lysosomal membrane proteins
    Eeva Liisa Eskelinen
    Department of Biochemistry, University of Kiel, Eduard Buchner Haus, Olshausenstrasse 40, D 24098 Kiel, Germany
    Trends Cell Biol 13:137-45. 2003
    ..The pivotal function of lysosomal membrane proteins is also highlighted by the recent identification of disease-causing mutations in cystine and sialic acid transporter proteins, leading to nephropathic cystinosis and Salla disease...
  12. ncbi request reprint Intravacuolar membrane lysis in Saccharomyces cerevisiae. Does vacuolar targeting of Cvt17/Aut5p affect its function?
    Ulrike D Epple
    University of Stuttgart, Institute of Biochemistry, Pfaffenwaldring 55, Germany
    J Biol Chem 278:7810-21. 2003
    ..Importantly, biochemical and morphological data further suggest involvement of Cvt17/Aut5p in disintegration of intravacuolar MVB vesicles. This points to a general function of Cvt17/Aut5p in intravacuolar membrane breakdown...
  13. ncbi request reprint Mannose 6-phosphate receptors, Niemann-Pick C2 protein, and lysosomal cholesterol accumulation
    Marion Willenborg
    Institute of Biochemistry, University of Kiel, D 24098 Kiel, Germany
    J Lipid Res 46:2559-69. 2005
    ..In addition, we observed an upregulation of NPC1 protein and mRNA in the MPR-double-deficient cells. Taken together, our results suggest that the lysosomal targeting of NPC2 is strictly dependent on MPRs in fibroblasts...
  14. ncbi request reprint LAMP-2: a control step for phagosome and autophagosome maturation
    Paul Saftig
    Biochemical Institute, Christian Albrechts University Kiel, Kiel, Germany
    Autophagy 4:510-2. 2008
    ..Recent studies in LAMP double-knockout fibroblasts suggests that LAMP-deficiency impairs the dynein-mediated transport of lysosomes to perinuclear regions where fusion with (auto)phagosomes occurs...
  15. ncbi request reprint 3D tomography reveals connections between the phagophore and endoplasmic reticulum
    Päivi Ylä-Anttila
    University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, Helsinki, Finland
    Autophagy 5:1180-5. 2009
    ..This finding has potential implications on the origin of autophagosome membranes, and on the mechanism of phagophore membrane extension. In addition, we observed lipid droplets in very close contact with the phagophores/autophagosomes...
  16. ncbi request reprint Maturation of autophagic vacuoles in Mammalian cells
    Eeva Liisa Eskelinen
    Institute of Biochemistry, University of Kiel, Germany
    Autophagy 1:1-10. 2005
    ..This suggests that autophagy has important housekeeping or protective functions because a block in autophagic maturation causes a disease...
  17. doi request reprint Fine structure of the autophagosome
    Eeva Liisa Eskelinen
    University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, Helsinki, Finland
    Methods Mol Biol 445:11-28. 2008
    ..Therefore, quantitation of autophagic vacuoles by electron microscopy and point counting is also described. Finally, a short description is given for preparation of ultra thin cryosections for immunogold labeling of autophagic vacuoles...
  18. doi request reprint New insights into the mechanisms of macroautophagy in mammalian cells
    Eeva Liisa Eskelinen
    Division of Biochemistry, Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
    Int Rev Cell Mol Biol 266:207-47. 2008
    ....
  19. ncbi request reprint Arrested maturation of Neisseria-containing phagosomes in the absence of the lysosome-associated membrane proteins, LAMP-1 and LAMP-2
    Marcelo G Binker
    Cell Biology Program, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    Cell Microbiol 9:2153-66. 2007
    ....
  20. pmc LAMP proteins are required for fusion of lysosomes with phagosomes
    Kassidy K Huynh
    Division of Cell Biology, The Hospital for Sick Children, Toronto, Canada
    EMBO J 26:313-24. 2007
    ..Time-lapse cinematography revealed that late endosomes/lysosomes as well as phagosomes lacking LAMP-1 and LAMP-2 had reduced ability to move toward the microtubule-organizing center, likely precluding their interaction with each other...
  21. ncbi request reprint A role for the lysosomal membrane protein LGP85 in the biogenesis and maintenance of endosomal and lysosomal morphology
    Toshio Kuronita
    Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi 3 1 1, Fukuoka 812 8582, Japan
    J Cell Sci 115:4117-31. 2002
    ..Thus, these findings provide important new insights into the role of LGP85 in the biogenesis and the maintenance of endosomes/lysosomes. We conclude that LGP85 may participate in reorganizing the endosomal/lysosomal compartments...
  22. ncbi request reprint The apoptosis/autophagy paradox: autophagic vacuolization before apoptotic death
    Rosa Ana Gonzalez-Polo
    CNRS UMR8125, Institut Gustave Roussy, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    J Cell Sci 118:3091-102. 2005
    ..Altogether, these data indicate that accumulation of autophagic vacuoles can precede apoptotic cell death. These findings argue against the clear-cut distinction between type 1 (apoptotic) and type 2 (autophagic) cell death...
  23. ncbi request reprint Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway
    Khuyen Meiling-Wesse
    Center of Biochemistry and Molecular Cell Biology, Georg August University, Heinrich Dueker Weg 12, D 37073 Goettingen, Germany
    J Biol Chem 280:33669-78. 2005
    ..But the phenotypes of trs85delta cells show striking differences to those seen in mutants with defects in the early secretory pathway. This suggests that Trs85 might play a direct role in the Cvt pathway and autophagy...
  24. doi request reprint Live longer with LAMP-2
    Paul Saftig
    Nat Med 14:909-10. 2008
  25. ncbi request reprint Inhibition of autophagy in mitotic animal cells
    Eeva Liisa Eskelinen
    Centre for High Resolution Imaging and Processing, MSI WTB complex, University of Dundee, School of Life Sciences, Dundee DD1 5EH, Scotland
    Traffic 3:878-93. 2002
    ..Our results show that autophagy is under strict mitotic control and indicate a novel role for phosphoinositide 3-kinases or other wortmannin/LY294002-sensitive kinases in mitotic membrane traffic regulation...
  26. ncbi request reprint Autophagy genes are essential for dauer development and life-span extension in C. elegans
    Alicia Melendez
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA
    Science 301:1387-91. 2003
    ..elegans orthologs of the yeast autophagy genes APG1, APG7, APG8, and AUT10. Thus, autophagy is a cellular pathway essential for dauer development and life-span extension in C. elegans...
  27. pmc Deafness in LIMP2-deficient mice due to early loss of the potassium channel KCNQ1/KCNE1 in marginal cells of the stria vascularis
    Marlies Knipper
    Biochemical Institute, Christian Albrechts University Kiel, Olshausenstr 40, D 24098 Kiel, Germany
    J Physiol 576:73-86. 2006
    ..Our findings suggest an important role for LIMP2 in the control of the localization and the level of apically expressed membrane proteins such as KCNQ1, KCNE1 and megalin in the stria vascularis...
  28. pmc Deletion of the SNARE vti1b in mice results in the loss of a single SNARE partner, syntaxin 8
    Vadim Atlashkin
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Mol Cell Biol 23:5198-207. 2003
    ..This suggests that other SNAREs can compensate for the reduction in syntaxin 8 and for the loss of vti1b in most mice even though vti1b shows only 30% amino acid identity with its closest relative...
  29. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
    ..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response...
  30. ncbi request reprint Impaired phagosomal maturation in neutrophils leads to periodontitis in lysosomal-associated membrane protein-2 knockout mice
    Wouter Beertsen
    Biochemical Institute, Christian Albrechts University Kiel, and Institute of Infection Medicine, University Hospital Schleswig Holstein, Germany
    J Immunol 180:475-82. 2008
    ..It also underscores the requirement of lysosomal fusion events to provide sufficient antimicrobial activity in PMNs, which is needed to prevent periodontal disease...
  31. pmc Calpain is required for macroautophagy in mammalian cells
    Francesca Demarchi
    Laboratorio Nazionale Consorzio Interuniversitario Biotecnologie, 34012 Trieste, Italy
    J Cell Biol 175:595-605. 2006
    ..In CAPNS1-depleted cells, ectopic LC3 accumulates in early endosome-like vesicles that may represent a salvage pathway for protein degradation when autophagy is defective...
  32. ncbi request reprint The mystery of the membranes
    Eeva Liisa Eskelinen
    Autophagy 4:3-4. 2008
  33. ncbi request reprint Calpain as a novel regulator of autophagosome formation
    Francesca Demarchi
    Laboratorio Nazionale Consorzio Interuniversitario Biotecnologie AREA Science Park, Padriciano, Italy
    Autophagy 3:235-7. 2007
    ..In Capns1-depleted cells ectopic LC3 accumulates in early endosome-like vesicles that might represent a salvage pathway for protein degradation when autophagy is defective...
  34. pmc Role of LAMP-2 in lysosome biogenesis and autophagy
    Eeva Liisa Eskelinen
    Centre for High Resolution Imaging and Processing, School of Life Sciences, University of Dundee, Scotland, UK
    Mol Biol Cell 13:3355-68. 2002
    ..Autophagic vacuoles may accumulate due to impaired capacity for lysosomal degradation...