Affiliation: University of Helsinki
Pietilä M, van Hemert M, Ahola T. Purification of highly active alphavirus replication complexes demonstrates altered fractionation of multiple cellular membranes. J Virol. 2018;: pubmed publisher
..We have here developed a gentle purification method to obtain large quantities of highly active replication complexes, and similar methods can be applied to other positive-strand RNA viruses. ..
Quirin T, Chen Y, Pietilä M, Guo D, Ahola T. The RNA Capping Enzyme Domain in Protein A is Essential for Flock House Virus Replication. Viruses. 2018;10: pubmed publisher
..Using fluorescent marker proteins, we demonstrated that both systems could replicate in the same cell. This work may facilitate the comparative analysis of FHV and SFV replication. ..
Ahola T, Karlin D. Sequence analysis reveals a conserved extension in the capping enzyme of the alphavirus supergroup, and a homologous domain in nodaviruses. Biol Direct. 2015;10:16 pubmed publisher
HellstrÃ¶m K, Vihinen H, Kallio K, Jokitalo E, Ahola T. Correlative light and electron microscopy enables viral replication studies at the ultrastructural level. Methods. 2015;90:49-56 pubmed publisher
..These results show that CLEM is a valuable technique for studying virus replication and membrane modifications at the ultrastructural level. ..
Kallio K, HellstrÃ¶m K, Jokitalo E, Ahola T. RNA Replication and Membrane Modification Require the Same Functions of Alphavirus Nonstructural Proteins. J Virol. 2016;90:1687-92 pubmed publisher
..Mutants capable of negative-strand synthesis, including those defective in RNA capping, gave rise to spherules. Recruitment of RNA to membranes in the absence of spherule formation was not detected. ..
Varghese F, Kaukinen P, GlÃ¤sker S, Bespalov M, Hanski L, Wennerberg K, et al
. Discovery of berberine, abamectin and ivermectin as antivirals against chikungunya and other alphaviruses. Antiviral Res. 2016;126:117-24 pubmed publisher
..Time of addition experiments also suggested that they act on the replication phase of the viral infectious cycle. ..
PietilÃ¤ M, HellstrÃ¶m K, Ahola T. Alphavirus polymerase and RNA replication. Virus Res. 2017;234:44-57 pubmed publisher
..Host factors offer an additional target for antivirals, as only few alphavirus polymerase inhibitors have been described. ..
Ahola T, Laakkonen P, Vihinen H, Kaariainen L. Critical residues of Semliki Forest virus RNA capping enzyme involved in methyltransferase and guanylyltransferase-like activities. J Virol. 1997;71:392-7 pubmed
..Secondary structure predictions of nsP1 and other proteins of the superfamily place these residues in positions corresponding to AdoMet-binding sites of cellular methyltransferases, suggesting that they all may be structurally related...
HellstrÃ¶m K, Kallio K, Utt A, Quirin T, Jokitalo E, Merits A, et al
. Partially Uncleaved Alphavirus Replicase Forms Spherule Structures in the Presence and Absence of RNA Template. J Virol. 2017;91: pubmed publisher
..Thus, partially cleaved replicase proteins play key roles that connect replication complex assembly, membrane deformation, and the different stages of RNA synthesis. ..