Ossi Turunen

Summary

Affiliation: Helsinki University of Technology
Country: Finland

Publications

  1. pmc In silico evidence for functional specialization after genome duplication in yeast
    Ossi Turunen
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Espoo, Finland
    FEMS Yeast Res 9:16-31. 2009
  2. ncbi request reprint Protein engineering: opportunities and challenges
    Matti Leisola
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Espoo, Finland
    Appl Microbiol Biotechnol 75:1225-32. 2007
  3. ncbi request reprint Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pH
    Ossi Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
    Protein Eng 15:141-5. 2002
  4. ncbi request reprint Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridges
    Hairong Xiong
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Helsinki, Finland
    Extremophiles 8:393-400. 2004
  5. ncbi request reprint Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis
    Ossi Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland
    Methods Enzymol 388:156-67. 2004
  6. ncbi request reprint Engineering the substrate specificity of xylose isomerase
    Johanna Karimäki
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT and Department of Chemistry, University of Joensuu, PO Box 111, 80101 Joensuu, Finland
    Protein Eng Des Sel 17:861-9. 2004
  7. pmc Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa
    Sasikala Anbarasan
    Department of Biotechnology and Chemical Technology, Aalto University, P O Box 6100, 02015 TKK, Espoo, Finland
    Appl Environ Microbiol 76:356-60. 2010

Collaborators

  • M Leisola
  • Fred Fenel
  • Johanna Karimäki
  • Sasikala Anbarasan
  • Hairong Xiong
  • Minna Vuolanto
  • Janne Jänis
  • Mikko Laitaoja
  • Pirjo Vainiotalo
  • Marja Paloheimo
  • Tarja Parkkinen
  • Juha Rouvinen
  • Harri Santa
  • Ossi Pastinen

Detail Information

Publications7

  1. pmc In silico evidence for functional specialization after genome duplication in yeast
    Ossi Turunen
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Espoo, Finland
    FEMS Yeast Res 9:16-31. 2009
    ..Our results indicate that subfunctionalization and even neofunctionalization has occurred along with degenerative evolution, in which unneeded functions were destroyed by mutations...
  2. ncbi request reprint Protein engineering: opportunities and challenges
    Matti Leisola
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Espoo, Finland
    Appl Microbiol Biotechnol 75:1225-32. 2007
    ..In the long run, random methods cannot replace insight in constructing life-like proteins. For the near future, however, in enzyme development, we need to rely on a combination of both...
  3. ncbi request reprint Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pH
    Ossi Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
    Protein Eng 15:141-5. 2002
    ..The stabilizing effect of arginines at high temperatures was seen clearly only when five arginines were introduced into the Ser/Thr surface...
  4. ncbi request reprint Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridges
    Hairong Xiong
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Helsinki, Finland
    Extremophiles 8:393-400. 2004
    ..The combination of disulphide bridges enhanced significantly the pH-dependent stability in a wide pH range...
  5. ncbi request reprint Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis
    Ossi Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland
    Methods Enzymol 388:156-67. 2004
  6. ncbi request reprint Engineering the substrate specificity of xylose isomerase
    Johanna Karimäki
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT and Department of Chemistry, University of Joensuu, PO Box 111, 80101 Joensuu, Finland
    Protein Eng Des Sel 17:861-9. 2004
    ..It improved 3-fold the catalytic efficiency of XI with L-arabinose; this increase was seen in both Km and kcat. This study showed that it is possible to engineer the substrate specificity of XI...
  7. pmc Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa
    Sasikala Anbarasan
    Department of Biotechnology and Chemical Technology, Aalto University, P O Box 6100, 02015 TKK, Espoo, Finland
    Appl Environ Microbiol 76:356-60. 2010
    ..In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH...