M Leisola

Summary

Affiliation: Helsinki University of Technology
Country: Finland

Publications

  1. ncbi request reprint Bioscience, bioinnovations, and bioethics
    Matti Leisola
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 6100, 02015 TKK, Helsinki, Finland
    Adv Biochem Eng Biotechnol 107:41-56. 2007
  2. ncbi request reprint Protein engineering: opportunities and challenges
    Matti Leisola
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Espoo, Finland
    Appl Microbiol Biotechnol 75:1225-32. 2007
  3. ncbi request reprint Production of xylitol from D-xylose by recombinant Lactococcus lactis
    Antti Nyyssölä
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, FIN 02015 Espoo, Finland
    J Biotechnol 118:55-66. 2005
  4. ncbi request reprint High-level production of D-mannitol with membrane cell-recycle bioreactor
    Niklas von Weymarn
    Laboratory of Bioprocess Engineering, Department of Chemical Engineering, Helsinki University of Technology, P O Box 6100, FIN 02015 HUT, Helsinki, Finland
    J Ind Microbiol Biotechnol 29:44-9. 2002
  5. ncbi request reprint Optimization of a Bifidobacterium longum production process
    Kristiina Kiviharju
    Helsinki University of Technology, Laboratory of Bioprocess Engineering, P O Box 6100, FIN 02015 HUT, Finland
    J Biotechnol 117:299-308. 2005
  6. doi request reprint Biotechnological production of L-ribose from L-arabinose
    M Helanto
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, PO Box 6100, 02015 Espoo, Finland
    Appl Microbiol Biotechnol 83:77-83. 2009
  7. doi request reprint Developing a multipoint titration method with a variable dose implementation for anaerobic digestion monitoring
    K Salonen
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Kemistintie 1, Espoo FI 02150, Finland
    Water Sci Technol 59:2395-403. 2009
  8. pmc Metabolic engineering of Lactobacillus plantarum for production of L-ribulose
    M Helanto
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, PO Box 6100, FIN 02015 Espoo, Finland
    Appl Environ Microbiol 73:7083-91. 2007
  9. ncbi request reprint On-line biomass measurements in bioreactor cultivations: comparison study of two on-line probes
    K Kiviharju
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 TKK Helsinki, Finland
    J Ind Microbiol Biotechnol 34:561-6. 2007
  10. ncbi request reprint Improved xylanase production by Trichoderma reesei grown on L-arabinose and lactose or D-glucose mixtures
    H Xiong
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 Espoo, Finland
    Appl Microbiol Biotechnol 64:353-8. 2004

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Bioscience, bioinnovations, and bioethics
    Matti Leisola
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 6100, 02015 TKK, Helsinki, Finland
    Adv Biochem Eng Biotechnol 107:41-56. 2007
    ..I shall finally discuss these problems from a historical perspective...
  2. ncbi request reprint Protein engineering: opportunities and challenges
    Matti Leisola
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Espoo, Finland
    Appl Microbiol Biotechnol 75:1225-32. 2007
    ..In the long run, random methods cannot replace insight in constructing life-like proteins. For the near future, however, in enzyme development, we need to rely on a combination of both...
  3. ncbi request reprint Production of xylitol from D-xylose by recombinant Lactococcus lactis
    Antti Nyyssölä
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, FIN 02015 Espoo, Finland
    J Biotechnol 118:55-66. 2005
    ..72 gl(-1)h(-1) at 20 h. Of the xylose initially present, 34% was consumed. Analysis of the fermentation metabolites revealed a shift from homolactic to mixed acid fermentation at early stages of the experiment...
  4. ncbi request reprint High-level production of D-mannitol with membrane cell-recycle bioreactor
    Niklas von Weymarn
    Laboratory of Bioprocess Engineering, Department of Chemical Engineering, Helsinki University of Technology, P O Box 6100, FIN 02015 HUT, Helsinki, Finland
    J Ind Microbiol Biotechnol 29:44-9. 2002
    ..Nitrogen gas flushing of the bioconversion media was unnecessary, since it did not change the essential process parameters...
  5. ncbi request reprint Optimization of a Bifidobacterium longum production process
    Kristiina Kiviharju
    Helsinki University of Technology, Laboratory of Bioprocess Engineering, P O Box 6100, FIN 02015 HUT, Finland
    J Biotechnol 117:299-308. 2005
    ..Temperature programming reduced drying times by over 50% and improved the product activity by over 160%...
  6. doi request reprint Biotechnological production of L-ribose from L-arabinose
    M Helanto
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, PO Box 6100, 02015 Espoo, Finland
    Appl Microbiol Biotechnol 83:77-83. 2009
    ..Also partially purified protein precipitates having both L-arabinose isomerase and L-ribose isomerase activity were successfully used for converting L-arabinose to L-ribose...
  7. doi request reprint Developing a multipoint titration method with a variable dose implementation for anaerobic digestion monitoring
    K Salonen
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Kemistintie 1, Espoo FI 02150, Finland
    Water Sci Technol 59:2395-403. 2009
    ..98 and 1.00 and average standard deviations of 0.6% and 0.8%, respectively. Furthermore, insensitivity of the presented method for overlapping buffer capacity curves was shown...
  8. pmc Metabolic engineering of Lactobacillus plantarum for production of L-ribulose
    M Helanto
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, PO Box 6100, FIN 02015 Espoo, Finland
    Appl Environ Microbiol 73:7083-91. 2007
    ..1 g liter(-1) h(-1), a best-achievable process productivity of 14.8 g liter(-1) h(-1), and a conversion of L-arabinose to L-ribulose of 0.70 mol mol(-1)...
  9. ncbi request reprint On-line biomass measurements in bioreactor cultivations: comparison study of two on-line probes
    K Kiviharju
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 TKK Helsinki, Finland
    J Ind Microbiol Biotechnol 34:561-6. 2007
    ..The possibilities offered by dielectric spectroscopy are impressive, but the on-line probe technology needs to be..
  10. ncbi request reprint Improved xylanase production by Trichoderma reesei grown on L-arabinose and lactose or D-glucose mixtures
    H Xiong
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 Espoo, Finland
    Appl Microbiol Biotechnol 64:353-8. 2004
    ....
  11. ncbi request reprint Engineering the substrate specificity of xylose isomerase
    Johanna Karimäki
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT and Department of Chemistry, University of Joensuu, PO Box 111, 80101 Joensuu, Finland
    Protein Eng Des Sel 17:861-9. 2004
    ..It improved 3-fold the catalytic efficiency of XI with L-arabinose; this increase was seen in both Km and kcat. This study showed that it is possible to engineer the substrate specificity of XI...
  12. ncbi request reprint A chemostat study of Streptomyces peucetius var. caesius N47
    Kristiina Kiviharju
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, Helsinki, P O Box 6100, 02015 HUT, Finland
    Appl Microbiol Biotechnol 73:1267-74. 2007
    ....
  13. ncbi request reprint Production of recombinant HIV-1 Nef (negative factor) protein using Pichia pastoris and a low-temperature fed-batch strategy
    Noora Sirén
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 6100, FI 02015 TKK, Finland
    Biotechnol Appl Biochem 44:151-8. 2006
    ..The purified product was characterized by SDS/PAGE, Western blotting, matrix-assisted laser-desorption ionization-time-of-flight MS, reversed-phase HPLC and N-terminal-sequence analysis...
  14. ncbi request reprint Modeling and simulation of Streptomyces peucetius var. caesius N47 cultivation and epsilon-rhodomycinone production with kinetic equations and neural networks
    Kristiina Kiviharju
    Helsinki University of Technology, Laboratory of Bioprocess Engineering, P O Box 6100, FIN 02015 TKK, Finland
    J Biotechnol 126:365-73. 2006
    ..903. The predictive power of the neural networks was superior to the kinetic models, which could not be used in predictive modeling of arbitrary batch cultivations...
  15. doi request reprint Factors affecting the production of L-xylulose by resting cells of recombinant Escherichia coli
    Anne Usvalampi
    Department of Biotechnology and Chemical Technology, Helsinki University of Technology, 02015 Espoo, Finland
    J Ind Microbiol Biotechnol 36:1323-30. 2009
    ..09 +/- 0.10 g g(-1) h(-1) was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy...
  16. doi request reprint A new and efficient phosphate starvation inducible expression system for Lactococcus lactis
    Noora Sirén
    Laboratory of Bioprocess Engineering, Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Espoo, Finland
    Appl Microbiol Biotechnol 79:803-10. 2008
    ..Furthermore, the system can be operated in L. lactis without introduction of regulatory genes into the host...
  17. ncbi request reprint Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridges
    Hairong Xiong
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Helsinki, Finland
    Extremophiles 8:393-400. 2004
    ..The combination of disulphide bridges enhanced significantly the pH-dependent stability in a wide pH range...
  18. ncbi request reprint Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pH
    Ossi Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
    Protein Eng 15:141-5. 2002
    ..The stabilizing effect of arginines at high temperatures was seen clearly only when five arginines were introduced into the Ser/Thr surface...
  19. ncbi request reprint Growth characteristics and oxidative capacity of Acetobacter aceti IFO 3281: implications for L-ribulose production
    A K Kylmä
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
    Appl Microbiol Biotechnol 63:584-91. 2004
    ..Based on the measured enzyme activities, metabolic pathways are proposed for ethanol and glycerol metabolism...
  20. ncbi request reprint Optimization of Streptomyces peucetius var. caesius N47 cultivation and epsilon-rhodomycinone production using experimental designs and response surface methods
    K Kiviharju
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PL 6100, 02015 HUT, Finland
    J Ind Microbiol Biotechnol 31:475-81. 2004
    ..A pH increase with a temperature decrease seemed most beneficial for productivity. This implies that dynamic control strategies in batch production of epsilon-rhodomycinone could increase the overall process productivity...
  21. pmc Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase
    A Nyyssola
    Helsinki University of Technology, Laboratory of Bioprocess Engineering, FIN 02015 HUT Espoo, Finland
    Appl Environ Microbiol 67:2044-50. 2001
    ..Both enzymes were strongly inhibited by the reaction product S-adenosylhomocysteine. Betaine inhibited the methylation reactions only at high concentrations...
  22. ncbi request reprint Metabolic flux analysis of Candida tropicalis growing on xylose in an oxygen-limited chemostat
    Tom Granström
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O B 6100, Kemistintie 1, FIN 02015 HUT, Finland
    Metab Eng 4:248-56. 2002
    ..Cofeeding of formate increased the ATP yield. The ATP yields of xylose and xylose-formate cultivation were 6.9 and 8.7 mol ATP/C-mol CDW, respectively, as calculated from the MFA...
  23. ncbi request reprint Chromatographic separation of nucleosides using a cross-linked xylose isomerase crystal stationary phase
    Jouni Jokela
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, FIN 02015 HUT, Finland
    J Sep Sci 27:1491-7. 2004
    ..Other commercially interesting sugar beet molasses components such as the acidic compounds betaine, gamma-amino butyric acid, and D- and L-pyroglutamic acids or neutral sucrose did not interact with the CLXIC material...
  24. ncbi request reprint Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis
    Ossi Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland
    Methods Enzymol 388:156-67. 2004
  25. ncbi request reprint Improved mannitol production by a random mutant of Leuconostoc pseudomesenteroides
    Miia Helanto
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 9400, FIN 02015 Espoo, Finland
    J Biotechnol 116:283-94. 2005
    ..Expression data with BPT143, revealing absence of fruK transcripts, was in accordance with the reduced fructokinase activity of the mutant...
  26. ncbi request reprint Growth characteristics and metabolic flux analysis of Candida milleri
    T B Granström
    Helsinki University of Technology, Department of Chemical Technology, Laboratory of Bioprocess Engineering, POB 6100, FIN 02015HUT, Finland
    Biotechnol Bioeng 70:197-207. 2000
    ....
  27. ncbi request reprint Effect of glycine betaine on osmoadaptation of Propionibacterium acidipropionici cultivated in elevated osmolarities
    A K Kylmä
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, HUT, Finland
    Appl Microbiol Biotechnol 54:705-10. 2000
    ..acidipropionici cells cultivated in the medium containing glycine betaine. The amount accumulated increased with NaCl concentration, suggesting that glycine betaine plays an important role in the osmoadaptation...
  28. ncbi request reprint Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis
    N von Weymarn
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, HUT, Finland
    Appl Microbiol Biotechnol 55:214-8. 2001
    ..Glycine betaine also stimulated the growth of E. coli and decreased acetate formation in mineral medium with high sucrose concentrations (up to 200 g.l(-1))...
  29. ncbi request reprint A combination of weakly stabilizing mutations with a disulfide bridge in the alpha-helix region of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermal stability through synergism
    O Turunen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015, HUT, Finland
    J Biotechnol 88:37-46. 2001
    ..There was no essential difference between the specific activities of the mutants and the wild-type XYNII...
  30. ncbi request reprint Light sensitivity of Bifidobacterium longum in bioreactor cultivations
    K Kiviharju
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, FIN 02015 HUT, Finland
    Biotechnol Lett 26:539-42. 2004
    ..36 h(-1) and 0.48 h(-1). In an illuminated bioreactor neither strain grew. In comparison, Lactobacillus reuteri was not sensitive to light under the same conditions...
  31. ncbi request reprint Bioreactor for solid-state cultivation of Phlebiopsis gigantea
    Veera Virtanen
    Laboratory of Bioprocess Engineering, Helsinki University of Technology, Espoo, Finland
    Biotechnol Lett 30:253-8. 2008
    ..Furthermore, the packed bed bioreactor was less time and space consuming and easier to operate than the tray bioreactor...
  32. ncbi request reprint Characterization of genes involved in fructose utilization by Lactobacillus fermentum
    Miia Helanto
    Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 9400, 02015 Espoo, Finland
    Arch Microbiol 186:51-9. 2006
    ..fermentum NRRL-B-1932 cells, which suggest that fructose is taken up by a permease system...
  33. ncbi request reprint Biodegradation of VOCs from printing press air by an on-site pilot plant bioscrubber and laboratory scale continuous yeast cultures
    Tom Granström
    Helsinki University of Technology, Laboratory of Bioprocess Engineering, Finland
    Biodegradation 13:155-62. 2002
    ..In laboratory scale the total and volumetric removal of VOCs by C. guilliermondii was more efficient compared to the pilot plant encouraging to scale up and applying the yeast bioreactor to real field conditions...
  34. pmc Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa
    Sasikala Anbarasan
    Department of Biotechnology and Chemical Technology, Aalto University, P O Box 6100, 02015 TKK, Espoo, Finland
    Appl Environ Microbiol 76:356-60. 2010
    ..In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH...
  35. ncbi request reprint Metabolic engineering of Lactobacillus fermentum for production of mannitol and pure L-lactic acid or pyruvate
    Johannes Aarnikunnas
    Faculty of Veterinary Medicine, Department of Basic Veterinary Sciences, Section of Microbiology, P O Box 57, FIN 00014 University of Helsinki, Finland
    Biotechnol Bioeng 82:653-63. 2003
    ..The double mutation negatively affected the glucose consumption rate, resulting in reduced cellular growth. In addition to pyruvate, the double mutant produced 2,3-butanediol. More surprisingly, some lactic acid was still produced...
  36. ncbi request reprint A de novo designed N-terminal disulphide bridge stabilizes the Trichoderma reesei endo-1,4-beta-xylanase II
    Fred Fenel
    Carbozyme Ltd, Keilaranta 16, 02150 Espoo, Finland
    J Biotechnol 108:137-43. 2004
    ..Our findings demonstrated that a properly designed disulphide bridge, here within the N-terminal region of XYNII, can be very effective in resisting thermal inactivation...
  37. ncbi request reprint A rare sugar xylitol. Part II: biotechnological production and future applications of xylitol
    Tom Birger Granström
    Rare Sugar Research Center, Kagawa University, Miki cho, Kagawa, 761 0795, Japan
    Appl Microbiol Biotechnol 74:273-6. 2007
    ..The second part of this mini-review on xylitol will look more closely at the biotechnological production and future applications of the rare sugar, xylitol...
  38. ncbi request reprint A rare sugar xylitol. Part I: the biochemistry and biosynthesis of xylitol
    Tom Birger Granström
    Rare Sugar Research Center, Kagawa University, Miki cho, Kagawa, 761 0795, Japan
    Appl Microbiol Biotechnol 74:277-81. 2007
    ..The first part of this mini-review concentrates on the biochemistry of xylitol biosynthesis and the problems related to intracellular redox balance...
  39. ncbi request reprint Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa. Comparison of twelve xylanases in relation to their thermal stability
    Nina Hakulinen
    Department of Chemistry, University of Joensuu, Finland Helsinki University of Technology, Finland
    Eur J Biochem 270:1399-412. 2003
    ....