Research Topics
| M LeisolaSummaryAffiliation: Helsinki University of Technology Country: Finland Publications
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Detail Information
Publications
Bioscience, bioinnovations, and bioethicsMatti Leisola
Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 6100, 02015 TKK, Helsinki, Finland
Adv Biochem Eng Biotechnol 107:41-56. 2007..I shall finally discuss these problems from a historical perspective...- Protein engineering: opportunities and challengesMatti Leisola
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Espoo, Finland
Appl Microbiol Biotechnol 75:1225-32. 2007..In the long run, random methods cannot replace insight in constructing life-like proteins. For the near future, however, in enzyme development, we need to rely on a combination of both... - Production of xylitol from D-xylose by recombinant Lactococcus lactisAntti Nyyssölä
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, FIN 02015 Espoo, Finland
J Biotechnol 118:55-66. 2005..72 gl(-1)h(-1) at 20 h. Of the xylose initially present, 34% was consumed. Analysis of the fermentation metabolites revealed a shift from homolactic to mixed acid fermentation at early stages of the experiment... - High-level production of D-mannitol with membrane cell-recycle bioreactorNiklas von Weymarn
Laboratory of Bioprocess Engineering, Department of Chemical Engineering, Helsinki University of Technology, P.O. Box 6100, FIN-02015 HUT, Helsinki, Finland
J Ind Microbiol Biotechnol 29:44-9. 2002..Nitrogen gas flushing of the bioconversion media was unnecessary, since it did not change the essential process parameters... - Optimization of a Bifidobacterium longum production processKristiina Kiviharju
Helsinki University of Technology, Laboratory of Bioprocess Engineering, P O Box 6100, FIN 02015 HUT, Finland
J Biotechnol 117:299-308. 2005..Temperature programming reduced drying times by over 50% and improved the product activity by over 160%... 
Biotechnological production of L-ribose from L-arabinoseM Helanto
Department of Biotechnology and Chemical Technology, Helsinki University of Technology, PO Box 6100, 02015 Espoo, Finland
Appl Microbiol Biotechnol 83:77-83. 2009..Also partially purified protein precipitates having both L-arabinose isomerase and L-ribose isomerase activity were successfully used for converting L-arabinose to L-ribose...- Developing a multipoint titration method with a variable dose implementation for anaerobic digestion monitoringK Salonen
Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Kemistintie 1, Espoo FI 02150, Finland
Water Sci Technol 59:2395-403. 2009..98 and 1.00 and average standard deviations of 0.6% and 0.8%, respectively. Furthermore, insensitivity of the presented method for overlapping buffer capacity curves was shown... 
Metabolic engineering of Lactobacillus plantarum for production of L-ribuloseM Helanto
Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, PO Box 6100, FIN 02015 Espoo, Finland
Appl Environ Microbiol 73:7083-91. 2007..1 g liter(-1) h(-1), a best-achievable process productivity of 14.8 g liter(-1) h(-1), and a conversion of L-arabinose to L-ribulose of 0.70 mol mol(-1)...- On-line biomass measurements in bioreactor cultivations: comparison study of two on-line probesK Kiviharju
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 TKK Helsinki, Finland
J Ind Microbiol Biotechnol 34:561-6. 2007..The possibilities offered by dielectric spectroscopy are impressive, but the on-line probe technology needs to be.. - Improved xylanase production by Trichoderma reesei grown on L-arabinose and lactose or D-glucose mixturesH Xiong
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, 02015 Espoo, Finland
Appl Microbiol Biotechnol 64:353-8. 2004.... - Engineering the substrate specificity of xylose isomeraseJohanna Karimäki
Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT and Department of Chemistry, University of Joensuu, PO Box 111, 80101 Joensuu, Finland
Protein Eng Des Sel 17:861-9. 2004..It improved 3-fold the catalytic efficiency of XI with L-arabinose; this increase was seen in both Km and kcat. This study showed that it is possible to engineer the substrate specificity of XI... - A chemostat study of Streptomyces peucetius var. caesius N47Kristiina Kiviharju
Laboratory of Bioprocess Engineering, Helsinki University of Technology, Helsinki, P O Box 6100, 02015 HUT, Finland
Appl Microbiol Biotechnol 73:1267-74. 2007.... - Production of recombinant HIV-1 Nef (negative factor) protein using Pichia pastoris and a low-temperature fed-batch strategyNoora Sirén
Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 6100, FI 02015 TKK, Finland
Biotechnol Appl Biochem 44:151-8. 2006..The purified product was characterized by SDS/PAGE, Western blotting, matrix-assisted laser-desorption ionization-time-of-flight MS, reversed-phase HPLC and N-terminal-sequence analysis... - Modeling and simulation of Streptomyces peucetius var. caesius N47 cultivation and epsilon-rhodomycinone production with kinetic equations and neural networksKristiina Kiviharju
Helsinki University of Technology, Laboratory of Bioprocess Engineering, P O Box 6100, FIN 02015 TKK, Finland
J Biotechnol 126:365-73. 2006..903. The predictive power of the neural networks was superior to the kinetic models, which could not be used in predictive modeling of arbitrary batch cultivations... - Factors affecting the production of L-xylulose by resting cells of recombinant Escherichia coliAnne Usvalampi
Department of Biotechnology and Chemical Technology, Helsinki University of Technology, 02015 Espoo, Finland
J Ind Microbiol Biotechnol 36:1323-30. 2009..09 +/- 0.10 g g(-1) h(-1) was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy... - A new and efficient phosphate starvation inducible expression system for Lactococcus lactisNoora Sirén
Laboratory of Bioprocess Engineering, Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Espoo, Finland
Appl Microbiol Biotechnol 79:803-10. 2008..Furthermore, the system can be operated in L. lactis without introduction of regulatory genes into the host... - Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridgesHairong Xiong
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, 02015-HUT, Helsinki, Finland
Extremophiles 8:393-400. 2004..The combination of disulphide bridges enhanced significantly the pH-dependent stability in a wide pH range... - Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pHOssi Turunen
Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
Protein Eng 15:141-5. 2002..The stabilizing effect of arginines at high temperatures was seen clearly only when five arginines were introduced into the Ser/Thr surface... - Growth characteristics and oxidative capacity of Acetobacter aceti IFO 3281: implications for L-ribulose productionA K Kylmä
Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
Appl Microbiol Biotechnol 63:584-91. 2004..Based on the measured enzyme activities, metabolic pathways are proposed for ethanol and glycerol metabolism... - Optimization of Streptomyces peucetius var. caesius N47 cultivation and epsilon-rhodomycinone production using experimental designs and response surface methodsK Kiviharju
Laboratory of Bioprocess Engineering, Helsinki University of Technology, PL 6100, 02015 HUT, Finland
J Ind Microbiol Biotechnol 31:475-81. 2004..A pH increase with a temperature decrease seemed most beneficial for productivity. This implies that dynamic control strategies in batch production of epsilon-rhodomycinone could increase the overall process productivity... - Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferaseA Nyyssola
Helsinki University of Technology, Laboratory of Bioprocess Engineering, FIN 02015 HUT Espoo, Finland
Appl Environ Microbiol 67:2044-50. 2001..Both enzymes were strongly inhibited by the reaction product S-adenosylhomocysteine. Betaine inhibited the methylation reactions only at high concentrations... - Metabolic flux analysis of Candida tropicalis growing on xylose in an oxygen-limited chemostatTom Granström
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O B 6100, Kemistintie 1, FIN 02015 HUT, Finland
Metab Eng 4:248-56. 2002..Cofeeding of formate increased the ATP yield. The ATP yields of xylose and xylose-formate cultivation were 6.9 and 8.7 mol ATP/C-mol CDW, respectively, as calculated from the MFA... - Chromatographic separation of nucleosides using a cross-linked xylose isomerase crystal stationary phaseJouni Jokela
Laboratory of Bioprocess Engineering, Helsinki University of Technology, FIN 02015 HUT, Finland
J Sep Sci 27:1491-7. 2004..Other commercially interesting sugar beet molasses components such as the acidic compounds betaine, gamma-amino butyric acid, and D- and L-pyroglutamic acids or neutral sucrose did not interact with the CLXIC material... - Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesisOssi Turunen
Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland
Methods Enzymol 388:156-67. 2004 - Improved mannitol production by a random mutant of Leuconostoc pseudomesenteroidesMiia Helanto
Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 9400, FIN 02015 Espoo, Finland
J Biotechnol 116:283-94. 2005..Expression data with BPT143, revealing absence of fruK transcripts, was in accordance with the reduced fructokinase activity of the mutant... - Growth characteristics and metabolic flux analysis of Candida milleriT B Granström
Helsinki University of Technology, Department of Chemical Technology, Laboratory of Bioprocess Engineering, POB 6100, FIN 02015HUT, Finland
Biotechnol Bioeng 70:197-207. 2000.... - Effect of glycine betaine on osmoadaptation of Propionibacterium acidipropionici cultivated in elevated osmolaritiesA K Kylmä
Laboratory of Bioprocess Engineering, Helsinki University of Technology, HUT, Finland
Appl Microbiol Biotechnol 54:705-10. 2000..acidipropionici cells cultivated in the medium containing glycine betaine. The amount accumulated increased with NaCl concentration, suggesting that glycine betaine plays an important role in the osmoadaptation... - Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesisN von Weymarn
Laboratory of Bioprocess Engineering, Helsinki University of Technology, HUT, Finland
Appl Microbiol Biotechnol 55:214-8. 2001..Glycine betaine also stimulated the growth of E. coli and decreased acetate formation in mineral medium with high sucrose concentrations (up to 200 g.l(-1))... - A combination of weakly stabilizing mutations with a disulfide bridge in the alpha-helix region of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermal stability through synergismO Turunen
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015, HUT, Finland
J Biotechnol 88:37-46. 2001..There was no essential difference between the specific activities of the mutants and the wild-type XYNII... - Light sensitivity of Bifidobacterium longum in bioreactor cultivationsK Kiviharju
Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, FIN 02015 HUT, Finland
Biotechnol Lett 26:539-42. 2004..36 h(-1) and 0.48 h(-1). In an illuminated bioreactor neither strain grew. In comparison, Lactobacillus reuteri was not sensitive to light under the same conditions... - Bioreactor for solid-state cultivation of Phlebiopsis giganteaVeera Virtanen
Laboratory of Bioprocess Engineering, Helsinki University of Technology, Espoo, Finland
Biotechnol Lett 30:253-8. 2008..Furthermore, the packed bed bioreactor was less time and space consuming and easier to operate than the tray bioreactor... - Characterization of genes involved in fructose utilization by Lactobacillus fermentumMiia Helanto
Laboratory of Bioprocess Engineering, Department of Chemical Technology, Helsinki University of Technology, P O Box 9400, 02015 Espoo, Finland
Arch Microbiol 186:51-9. 2006..fermentum NRRL-B-1932 cells, which suggest that fructose is taken up by a permease system... - Biodegradation of VOCs from printing press air by an on-site pilot plant bioscrubber and laboratory scale continuous yeast culturesTom Granström
Helsinki University of Technology, Laboratory of Bioprocess Engineering, Finland
Biodegradation 13:155-62. 2002..In laboratory scale the total and volumetric removal of VOCs by C. guilliermondii was more efficient compared to the pilot plant encouraging to scale up and applying the yeast bioreactor to real field conditions... - Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosaSasikala Anbarasan
Department of Biotechnology and Chemical Technology, Aalto University, P O Box 6100, 02015 TKK, Espoo, Finland
Appl Environ Microbiol 76:356-60. 2010..In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH... - Metabolic engineering of Lactobacillus fermentum for production of mannitol and pure L-lactic acid or pyruvateJohannes Aarnikunnas
Faculty of Veterinary Medicine, Department of Basic Veterinary Sciences, Section of Microbiology, P.O. Box 57, FIN-00014 University of Helsinki, Finland
Biotechnol Bioeng 82:653-63. 2003..The double mutation negatively affected the glucose consumption rate, resulting in reduced cellular growth. In addition to pyruvate, the double mutant produced 2,3-butanediol. More surprisingly, some lactic acid was still produced... - A de novo designed N-terminal disulphide bridge stabilizes the Trichoderma reesei endo-1,4-beta-xylanase IIFred Fenel
Carbozyme Ltd, Keilaranta 16, 02150 Espoo, Finland
J Biotechnol 108:137-43. 2004..Our findings demonstrated that a properly designed disulphide bridge, here within the N-terminal region of XYNII, can be very effective in resisting thermal inactivation... - A rare sugar xylitol. Part II: biotechnological production and future applications of xylitolTom Birger Granström
Rare Sugar Research Center, Kagawa University, Miki cho, Kagawa, 761 0795, Japan
Appl Microbiol Biotechnol 74:273-6. 2007..The second part of this mini-review on xylitol will look more closely at the biotechnological production and future applications of the rare sugar, xylitol... - A rare sugar xylitol. Part I: the biochemistry and biosynthesis of xylitolTom Birger Granström
Rare Sugar Research Center, Kagawa University, Miki cho, Kagawa, 761 0795, Japan
Appl Microbiol Biotechnol 74:277-81. 2007..The first part of this mini-review concentrates on the biochemistry of xylitol biosynthesis and the problems related to intracellular redox balance... - Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa. Comparison of twelve xylanases in relation to their thermal stabilityNina Hakulinen
Department of Chemistry, University of Joensuu, Finland Helsinki University of Technology, Finland
Eur J Biochem 270:1399-412. 2003....