Alessandro Esposito

Summary

Publications

  1. ncbi request reprint alpha-Synuclein and its disease-related mutants interact differentially with the microtubule protein tau and associate with the actin cytoskeleton
    Alessandro Esposito
    Cell Biophysics Group at the European Neuroscience Institute Göttingen, Waldweg, 33, 37073 Gottingen, Germany
    Neurobiol Dis 26:521-31. 2007
  2. pmc The homeostasis of Plasmodium falciparum-infected red blood cells
    Jakob M A Mauritz
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    PLoS Comput Biol 5:e1000339. 2009
  3. doi request reprint pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging
    Alessandro Esposito
    Laboratory for Molecular and Cellular Systems, Department of Neuro and Sensory Physiology, University Medicine Gottingen, Humboldtalee 23, 37073 Gottingen, Germany
    Biochemistry 47:13115-26. 2008
  4. pmc Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis
    Alessandro Esposito
    Cell Biophysics Group, European Neuroscience Institute, 37073 Gottingen, Germany
    Biophys J 89:4286-99. 2005
  5. ncbi request reprint Design and application of a confocal microscope for spectrally resolved anisotropy imaging
    Alessandro Esposito
    The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK
    Opt Express 19:2546-55. 2011
  6. pmc FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells
    Alessandro Esposito
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 3:e3780. 2008
  7. ncbi request reprint Unsupervised fluorescence lifetime imaging microscopy for high content and high throughput screening
    Alessandro Esposito
    Cell Biophysics Group, European Neuroscience Institute Göttingen, Waldweg 33, 37073 Gottingen, Germany
    Mol Cell Proteomics 6:1446-54. 2007
  8. pmc Quantitative imaging of human red blood cells infected with Plasmodium falciparum
    Alessandro Esposito
    Department of Chemical Engineering, and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Biophys J 99:953-60. 2010
  9. pmc X-ray microanalysis investigation of the changes in Na, K, and hemoglobin concentration in plasmodium falciparum-infected red blood cells
    Jakob M A Mauritz
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Biophys J 100:1438-45. 2011
  10. doi request reprint Quantitative fluorescence microscopy techniques
    Alessandro Esposito
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Methods Mol Biol 586:117-42. 2009

Collaborators

Detail Information

Publications22

  1. ncbi request reprint alpha-Synuclein and its disease-related mutants interact differentially with the microtubule protein tau and associate with the actin cytoskeleton
    Alessandro Esposito
    Cell Biophysics Group at the European Neuroscience Institute Göttingen, Waldweg, 33, 37073 Gottingen, Germany
    Neurobiol Dis 26:521-31. 2007
    ..Given the concurrent loss of membrane binding by this mutation, we propose a membrane-bound functional complex with tau that might involve the actin cytoskeleton...
  2. pmc The homeostasis of Plasmodium falciparum-infected red blood cells
    Jakob M A Mauritz
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    PLoS Comput Biol 5:e1000339. 2009
    ..The conclusions suggest that infected red cells do reach proximity to lytic rupture regardless of their actual volume, thus requiring a progressive reduction in their hemoglobin concentration to prevent premature lysis...
  3. doi request reprint pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging
    Alessandro Esposito
    Laboratory for Molecular and Cellular Systems, Department of Neuro and Sensory Physiology, University Medicine Gottingen, Humboldtalee 23, 37073 Gottingen, Germany
    Biochemistry 47:13115-26. 2008
    ..The principle is demonstrated in the existing high-efficiency FRET fusion Cy11.5, and tunability of the platform design is demonstrated by genetic alteration of the pH sensitivity of the acceptor moiety...
  4. pmc Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis
    Alessandro Esposito
    Cell Biophysics Group, European Neuroscience Institute, 37073 Gottingen, Germany
    Biophys J 89:4286-99. 2005
    ..Finally, a new method to resolve a FRET system is proposed and experimentally applied to the investigation of protein-protein interactions...
  5. ncbi request reprint Design and application of a confocal microscope for spectrally resolved anisotropy imaging
    Alessandro Esposito
    The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK
    Opt Express 19:2546-55. 2011
    ..We demonstrate the novel optical design with example images of plant cells and of mammalian cells expressing fluorescent proteins undergoing energy transfer...
  6. pmc FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells
    Alessandro Esposito
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 3:e3780. 2008
    ..Critical for this hypothesis was that the hemoglobin concentration within the host cell be progressively reduced from the trophozoite stage onwards...
  7. ncbi request reprint Unsupervised fluorescence lifetime imaging microscopy for high content and high throughput screening
    Alessandro Esposito
    Cell Biophysics Group, European Neuroscience Institute Göttingen, Waldweg 33, 37073 Gottingen, Germany
    Mol Cell Proteomics 6:1446-54. 2007
    ..The finding of statistically lower ubiquitination of the mutant alpha-synuclein forms supports a role for this modification in the mechanism of pathological protein aggregation...
  8. pmc Quantitative imaging of human red blood cells infected with Plasmodium falciparum
    Alessandro Esposito
    Department of Chemical Engineering, and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Biophys J 99:953-60. 2010
    ..These results support the general validity of the colloidosmotic hypothesis, settle the IRBC volume debate, and help to constrain the range of parameter values in the numerical model...
  9. pmc X-ray microanalysis investigation of the changes in Na, K, and hemoglobin concentration in plasmodium falciparum-infected red blood cells
    Jakob M A Mauritz
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Biophys J 100:1438-45. 2011
    ..The results also provide, for the first time to our knowledge, comprehensive maps of the elemental distributions of Na, K, and Fe in falciparum-infected red blood cells...
  10. doi request reprint Quantitative fluorescence microscopy techniques
    Alessandro Esposito
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Methods Mol Biol 586:117-42. 2009
    ..Quantitative fluorescence microscopy techniques offer powerful tools for understanding the physiological and pathological roles of molecular machineries in the living cell...
  11. doi request reprint Biophotonic techniques for the study of malaria-infected red blood cells
    Jakob M A Mauritz
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Med Biol Eng Comput 48:1055-63. 2010
    ..The combined application of these techniques sheds new light on the detailed mechanisms of malaria infection providing potential for new diagnostic or therapeutic approaches...
  12. ncbi request reprint Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics
    Alessandro Esposito
    European Neuroscience Institute, Gottingen, Cell Biophysics Group, Germany
    J Biomed Opt 11:34016. 2006
    ..The all-solid-state design bridges the technological gap that limits the use of FLIM in areas such as drug discovery and medical diagnostics...
  13. ncbi request reprint A FRET sensor for non-invasive imaging of amyloid formation in vivo
    Gabriele S Kaminski Schierle
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge CB2 3RA, UK
    Chemphyschem 12:673-80. 2011
    ....
  14. pmc Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication
    Winsome Cheung
    Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge CB2 0QQ, United Kingdom
    J Virol 84:6782-98. 2010
    ..Rotaviruses represent a genus of another virus family utilizing LD components for replication, pointing at novel therapeutic targets for these pathogens...
  15. pmc DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells
    Anand D Jeyasekharan
    The Medical Research Council Cancer Cell Unit, Hutchison Medical Research Council Research Centre, Cambridge CB2 0XZ, United Kingdom
    Proc Natl Acad Sci U S A 107:21937-42. 2010
    ..Our findings identify signal-regulated changes in nucleoplasmic protein diffusion as a means to control biochemical reactions in the cell nucleus...
  16. pmc Synaptic scaffolding protein SYD-2 clusters and activates kinesin-3 UNC-104 in C. elegans
    Oliver I Wagner
    DFG Research Center for Molecular Physiology of the Brain CMPB, Georg August University, 37073 Gottingen, Germany
    Proc Natl Acad Sci U S A 106:19605-10. 2009
    ..These data present a kinesin scaffolding protein that controls both motor clustering along axons and motor motility, resulting in reduced cargo transport efficiency upon loss of interaction...
  17. ncbi request reprint Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing
    Hongqing Liang
    1 Medical Research Council Cancer Unit, University of Cambridge, Hills Road, Cambridge CB2 0XZ, UK 2 Institute of Molecular and Cell Biology, Agency for Science Technology and Research, Biopolis Drive, Biopolis Way, Singapore 138673 3 Bioprocessing Technology Institute, Agency for Science Technology and Research, Biopolis Way, Singapore 138668
    Nat Commun 5:4048. 2014
    ....
  18. pmc Maximizing the biochemical resolving power of fluorescence microscopy
    Alessandro Esposito
    Medical Research Council Cancer Unit, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 8:e77392. 2013
    ....
  19. doi request reprint Fluorescence lifetime imaging microscopy
    Alessandro Esposito
    European Neuroscience Institute Goetingen, Goetingen, Germany
    Curr Protoc Cell Biol . 2004
    ..The availability of different spectral variants of the visible fluorescent proteins (VFPs) allows the investigation of molecular activities and protein-protein interactions in living cells by FRET as measured by FLIM...
  20. ncbi request reprint Bax inhibitor-1 protects neurons from oxygen-glucose deprivation
    Christoph P Dohm
    Department of Neurology, University of Gottingen, 37075 Gottingen, Germany
    J Mol Neurosci 29:1-8. 2006
    ..Taken together, BI-1 overexpression in the ER is protective in neurons, making BI-1 an interesting target for future studies aiming at the inhibition of neuronal cell death during neurodegenerative diseases and stroke...
  21. ncbi request reprint Two-photon analysis of lead accumulation in rat cerebellar granule neurons
    Alessandro Esposito
    INFM, Dipartimento di Fisica, Universita di Genova, Via Dodecaneso, 33, 16146, Genova, Italy
    Neurochem Res 30:949-54. 2005
    ..Intracellular Pb2+ concentrations were evaluated from the fluorescence intensity and this estimate indicated that the concentration of free Pb2+ sufficient to inactivate the transport system is close to 50 pM...
  22. ncbi request reprint Two-photon imaging of calcium accumulation in rat cerebellar granule cells
    Francesca Pellistri
    INFM, Dipartimento di Fisica, Universita di Genova, Via Dodecaneso 33, 16146 Genova, Italy
    Neuroreport 15:83-7. 2004
    ..In the latter, the response to NMDA treatment was smaller and heterogeneous, and [Ca2+]i increased in certain segments of the neurite, but not in others...