Vincent Gh Eijsink
Kojima Y, VÃ¡rnai A, Ishida T, Sunagawa N, Petrović D, Igarashi K, et al
. A Lytic Polysaccharide Monooxygenase with Broad Xyloglucan Specificity from the Brown-Rot Fungus Gloeophyllum trabeum and Its Action on Cellulose-Xyloglucan Complexes. Appl Environ Microbiol. 2016;82:6557-6572 pubmed
..The xyloglucan-degrading potential of GtLPMO9A-2 suggests a role in decreasing wood strength at the initial stage of brown rot through degradation of the primary cell wall. ..
Várnai A, Umezawa K, Yoshida M, Eijsink V. The Pyrroloquinoline-Quinone-Dependent Pyranose Dehydrogenase from Coprinopsis cinerea Drives Lytic Polysaccharide Monooxygenase Action. Appl Environ Microbiol. 2018;84: pubmed publisher
Forsberg Z, Sørlie M, Petrovic D, Courtade G, Aachmann F, Vaaje Kolstad G, et al
. Polysaccharide degradation by lytic polysaccharide monooxygenases. Curr Opin Struct Biol. 2019;59:54-64 pubmed publisher
..Furthermore, it has been shown that LPMOs may carry out peroxygenase reactions, at much higher rates than monooxygenase reactions, which affects our understanding and exploitation of these powerful enzymes. ..
Fredriksen L, Stokke R, Jensen M, Westereng B, Jameson J, Steen I, et al
. Discovery of a Thermostable GH10 Xylanase with Broad Substrate Specificity from the Arctic Mid-Ocean Ridge Vent System. Appl Environ Microbiol. 2019;85: pubmed publisher
..Thus, this N-terminal domain lays the foundation for the new CBM85 family. ..
Hegnar O, Petrović D, Bissaro B, Alfredsen G, Várnai A, Eijsink V. pH-Dependent Relationship between Catalytic Activity and Hydrogen Peroxide Production Shown via Characterization of a Lytic Polysaccharide Monooxygenase from Gloeophyllum trabeum. Appl Environ Microbiol. 2019;85: pubmed publisher
..Finally, we identified a reductant that leads to enzyme activation without any endogenous H2O2 generation, allowing for improved control of LPMO reactivity and providing a valuable tool for future LPMO research. ..
Michon C, Christophe M, Kuczkowska K, Langella P, Eijsink V, Mathiesen G, et al
. Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo. Microb Cell Fact. 2015;14:95 pubmed publisher
..It is likely that the more embedded localization of aDec in the latter case is favorable when cells are exposed to the harsh conditions of the gastro-intestinal tract. ..
Westereng B, Loose J, Vaaje Kolstad G, Aachmann F, Sørlie M, Eijsink V. Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs). Methods Mol Biol. 2018;1796:219-246 pubmed publisher
..In some cases, substrate affinity can be assessed using isothermal titration calorimetry. These methods are described in this chapter. ..
Pechsrichuang P, Lorentzen S, Aam B, Tuveng T, Hamre A, Eijsink V, et al
. Bioconversion of chitosan into chito-oligosaccharides (CHOS) using family 46 chitosanase from Bacillus subtilis (BsCsn46A). Carbohydr Polym. 2018;186:420-428 pubmed publisher
..The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies. ..
Forsberg Z, MacKenzie A, Sørlie M, Røhr A, Helland R, Arvai A, et al
. Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases. Proc Natl Acad Sci U S A. 2014;111:8446-51 pubmed publisher
..During cellulose degradation, the members of this LPMO pair act in synergy, indicating different functional roles and providing a rationale for the abundance of these enzymes in biomass-degrading organisms. ..