Ellen G Duysen

Summary

Publications

  1. pmc Differential sensitivity of plasma carboxylesterase-null mice to parathion, chlorpyrifos and chlorpyrifos oxon, but not to diazinon, dichlorvos, diisopropylfluorophosphate, cresyl saligenin phosphate, cyclosarin thiocholine, tabun thiocholine, and carbofu
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Chem Biol Interact 195:189-98. 2012
  2. ncbi request reprint Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Biochem Pharmacol 70:1673-84. 2005
  3. ncbi request reprint The butyrylcholinesterase knockout mouse a research tool in the study of drug sensitivity, bio-distribution, obesity and Alzheimer's disease
    Ellen G Duysen
    Researcher Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Expert Opin Drug Metab Toxicol 5:523-8. 2009
  4. pmc Mice treated with chlorpyrifos or chlorpyrifos oxon have organophosphorylated tubulin in the brain and disrupted microtubule structures, suggesting a role for tubulin in neurotoxicity associated with exposure to organophosphorus agents
    Wei Jiang
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 5950, USA
    Toxicol Sci 115:183-93. 2010
  5. pmc Mass spectrometry identifies multiple organophosphorylated sites on tubulin
    Hasmik Grigoryan
    University of Nebraska Medical Center, Eppley Institute for Cancer Research, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 240:149-58. 2009
  6. ncbi request reprint Screening assays for cholinesterases resistant to inhibition by organophosphorus toxicants
    Yuxia Wang
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Anal Biochem 329:131-8. 2004
  7. ncbi request reprint Increased hepatotoxicity and cardiac fibrosis in cocaine-treated butyrylcholinesterase knockout mice
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Basic Clin Pharmacol Toxicol 103:514-21. 2008
  8. ncbi request reprint Gene transfer of acetylcholinesterase protects the knockout mouse from the toxicity of DFP
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    J Mol Neurosci 30:79-80. 2006
  9. ncbi request reprint Protection from the toxicity of diisopropylfluorophosphate by adeno-associated virus expressing acetylcholinesterase
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 214:152-65. 2006
  10. ncbi request reprint Sensitivity of butyrylcholinesterase knockout mice to (--)-huperzine A and donepezil suggests humans with butyrylcholinesterase deficiency may not tolerate these Alzheimer's disease drugs and indicates butyrylcholinesterase function in neurotransmission
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicology 233:60-9. 2007

Collaborators

Detail Information

Publications27

  1. pmc Differential sensitivity of plasma carboxylesterase-null mice to parathion, chlorpyrifos and chlorpyrifos oxon, but not to diazinon, dichlorvos, diisopropylfluorophosphate, cresyl saligenin phosphate, cyclosarin thiocholine, tabun thiocholine, and carbofu
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Chem Biol Interact 195:189-98. 2012
    ....
  2. ncbi request reprint Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Biochem Pharmacol 70:1673-84. 2005
    ..Since human plasma contains no carboxylesterase, only BChE, PON1, and albumin esterases need to be considered when evaluating hydrolysis of an ester drug in human plasma...
  3. ncbi request reprint The butyrylcholinesterase knockout mouse a research tool in the study of drug sensitivity, bio-distribution, obesity and Alzheimer's disease
    Ellen G Duysen
    Researcher Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Expert Opin Drug Metab Toxicol 5:523-8. 2009
    ..Knowledge of drug sensitivities in the mouse model of human BChE deficiency will aid in understanding adverse drug effects in humans...
  4. pmc Mice treated with chlorpyrifos or chlorpyrifos oxon have organophosphorylated tubulin in the brain and disrupted microtubule structures, suggesting a role for tubulin in neurotoxicity associated with exposure to organophosphorus agents
    Wei Jiang
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 5950, USA
    Toxicol Sci 115:183-93. 2010
    ..Covalent binding of CPO to tubulin and to tubulin-associated proteins is a potential mechanism of neurotoxicity...
  5. pmc Mass spectrometry identifies multiple organophosphorylated sites on tubulin
    Hasmik Grigoryan
    University of Nebraska Medical Center, Eppley Institute for Cancer Research, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 240:149-58. 2009
    ..In conclusion seventeen tyrosines in tubulin have the potential to covalently bind chlorpyrifos oxon. These results will be useful when searching for OP-labeled tubulin in live animals...
  6. ncbi request reprint Screening assays for cholinesterases resistant to inhibition by organophosphorus toxicants
    Yuxia Wang
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Anal Biochem 329:131-8. 2004
    ..Both wild-type and G117H BChE were in the epithelial cells of the villi. These assays can be used to identify OP-resistant cholinesterases in culture medium and in animal tissues...
  7. ncbi request reprint Increased hepatotoxicity and cardiac fibrosis in cocaine-treated butyrylcholinesterase knockout mice
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Basic Clin Pharmacol Toxicol 103:514-21. 2008
    ..The observed functional changes following acute high-dose and chronic low-dose cocaine in BChE-/- and +/- mice warrants further investigation into the possibility of increased cocaine toxicity in human beings with BChE deficiency...
  8. ncbi request reprint Gene transfer of acetylcholinesterase protects the knockout mouse from the toxicity of DFP
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    J Mol Neurosci 30:79-80. 2006
    ..We used the AChE-/- mouse for these studies because this mouse has no endogenous AChE activity (Xie et al., 2000). Any AChE activity found in tissues could only come from the viral vector...
  9. ncbi request reprint Protection from the toxicity of diisopropylfluorophosphate by adeno-associated virus expressing acetylcholinesterase
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 214:152-65. 2006
    ..We conclude that AChE scavenged OP and in this way protected the activity of butyrylcholinesterase (BChE, EC 3.1.1.8) in motor endplates...
  10. ncbi request reprint Sensitivity of butyrylcholinesterase knockout mice to (--)-huperzine A and donepezil suggests humans with butyrylcholinesterase deficiency may not tolerate these Alzheimer's disease drugs and indicates butyrylcholinesterase function in neurotransmission
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Toxicology 233:60-9. 2007
    ..Thus, BChE has a function in neurotransmission. People with BChE deficiency are expected to be intolerant of standard doses of the anti-Alzheimer's drugs, (--)-huperzine A and donepezil...
  11. ncbi request reprint Phenotype comparison of three acetylcholinesterase knockout strains
    Ellen G Duysen
    University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Mol Neurosci 30:91-2. 2006
    ..AChE-/- mice in strains C57 and CD1died during seizures before postnatal day (P) 21, whereas mice in strain 129/Sv lived to adulthood...
  12. pmc Production of ES1 plasma carboxylesterase knockout mice for toxicity studies
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 5950, USA
    Chem Res Toxicol 24:1891-8. 2011
    ..The ES1-/- mouse should be an appropriate model for testing highly toxic nerve agents and for evaluating protection strategies against the toxicity of nerve agents...
  13. ncbi request reprint Albumin, a new biomarker of organophosphorus toxicant exposure, identified by mass spectrometry
    Eric S Peeples
    University of Nebraska Medical Center, Eppley Institute, Omaha, Nebraska 68198 6805, USA
    Toxicol Sci 83:303-12. 2005
    ..Carboxylesterase is not a biomarker in man because humans have no carboxylesterase in blood. It is concluded that OP bound to albumin could serve as a new biomarker of OP exposure in man...
  14. doi request reprint The butyrylcholinesterase knockout mouse is obese on a high-fat diet
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Chem Biol Interact 175:88-91. 2008
    ..Their obesity was not explained by increased levels of octanoyl-ghrelin, or by increased caloric intake, or by decreased exercise. Instead, a role for BChE in fat utilization was suggested...
  15. doi request reprint Mice treated with a nontoxic dose of chlorpyrifos oxon have diethoxyphosphotyrosine labeled proteins in blood up to 4 days post exposure, detected by mass spectrometry
    Wei Jiang
    Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center, Omaha, NE 68198 5950, United States
    Toxicology 295:15-22. 2012
    ..In contrast plasma AChE activity returned to normal after 24-72 h. In conclusion MALDI-TOF mass spectrometry can be used to diagnose exposure to chlorpyrifos oxon days after AChE inhibition assays are uninformative...
  16. ncbi request reprint Induction of plasma acetylcholinesterase activity in mice challenged with organophosphorus poisons
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Toxicol Appl Pharmacol 255:214-20. 2011
    ..We hypothesize that acetylcholinesterase activity is induced when cells undergo apoptosis and that consequently there is a rise in the level of plasma acetylcholinesterase...
  17. ncbi request reprint Production of the butyrylcholinesterase knockout mouse
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    J Mol Neurosci 30:193-5. 2006
    ..The BChE knockout mouse will allow us to test the hypothesis that the function of BChE is to detoxify poisons and will allow us to test the role of BChE in other physiological functions...
  18. pmc Polyclonal antibody to soman-tyrosine
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 5950, United States
    Chem Res Toxicol 26:584-92. 2013
    ..In conclusion, a high-affinity, polyclonal antibody that specifically recognizes soman adducts on tyrosine in a variety of proteins has been produced. Such an antibody could be useful for identifying secondary targets of soman toxicity...
  19. ncbi request reprint Delivery of human acetylcholinesterase by adeno-associated virus to the acetylcholinesterase knockout mouse
    Anna Hrabovska
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Chem Biol Interact 157:71-8. 2005
    ..This supports the hypothesis that their seizures are induced by excess acetylcholine...
  20. ncbi request reprint The butyrylcholinesterase knockout mouse as a model for human butyrylcholinesterase deficiency
    Bin Li
    Eppley Institute, 986805, Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Pharmacol Exp Ther 324:1146-54. 2008
    ..p., induced tonicclonic convulsions and death in BChE(-/-) mice. This suggests that butyrylcholine, like pilocarpine, binds to muscarinic receptors. In conclusion, the BChE(-/-) mouse is a suitable model for human BChE deficiency...
  21. ncbi request reprint Regulation of muscarinic acetylcholine receptor function in acetylcholinesterase knockout mice
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Pharmacol Biochem Behav 74:977-86. 2003
    ..However, mRNA levels for muscarinic receptors were unchanged. These results indicate that one adaptation to the absence of AChE is downregulation of muscarinic receptors, thus reducing response to cholinergic stimulation...
  22. pmc Intrathecal delivery of fluorescent labeled butyrylcholinesterase to the brains of butyrylcholinesterase knock-out mice: visualization and quantification of enzyme distribution in the brain
    Noel D Johnson
    Division of Comparative Medicine, University of Nebraska Medical Center, Omaha, NE 68198, United States
    Neurotoxicology 30:386-92. 2009
    ..This procedure proved to be an efficient, safe and rapid method to deliver BChE to the CNS of mice, providing a research tool for determining neural protection by BChE following OP exposure...
  23. doi request reprint Whole body and tissue imaging of the butyrylcholinesterase knockout mouse injected with near infrared dye labeled butyrylcholinesterase
    Ellen G Duysen
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Chem Biol Interact 175:119-24. 2008
    ..It is concluded that the tetrameric BChE glycoprotein of 340 kDa diffuses from the muscle injection site to blood and peripheral organs and has a longer residence time in the organs than in blood...
  24. doi request reprint Prolonged toxic effects after cocaine challenge in butyrylcholinesterase/plasma carboxylesterase double knockout mice: a model for butyrylcholinesterase-deficient humans
    Ellen G Duysen
    University of Nebraska Medical Center, Eppley Institute, 985950 Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Drug Metab Dispos 39:1321-3. 2011
    ....
  25. ncbi request reprint Resistance to organophosphorus agent toxicity in transgenic mice expressing the G117H mutant of human butyrylcholinesterase
    Yuxia Wang
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Toxicol Appl Pharmacol 196:356-66. 2004
    ..In conclusion, the transgenic G117H BChE mouse demonstrates the factors required to achieve protection from OP toxicity in a vertebrate animal...
  26. ncbi request reprint Wild-type and A328W mutant human butyrylcholinesterase tetramers expressed in Chinese hamster ovary cells have a 16-hour half-life in the circulation and protect mice from cocaine toxicity
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, 986806 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Pharmacol Exp Ther 302:751-8. 2002
    ..8 units/g i.p. reduced cocaine-induced locomotor activity by 50 and 80%. These results indicate that recombinant human BChE could be useful for treating cocaine toxicity in humans...
  27. ncbi request reprint Rescue of the acetylcholinesterase knockout mouse by feeding a liquid diet; phenotype of the adult acetylcholinesterase deficient mouse
    Ellen G Duysen
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Brain Res Dev Brain Res 137:43-54. 2002
    ..These findings support the classical role for AChE in nerve impulse conduction and further suggest that AChE is essential for timely physical development and higher brain function...