Kevin M Downard

Summary

Publications

  1. pmc Proteotyping to establish gene origin within reassortant influenza viruses
    Ji Won Ha
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 6:e15771. 2011
  2. ncbi Proteotyping for the rapid identification of influenza virus and other biopathogens
    Kevin M Downard
    School of Molecular Bioscience G 08, University of Sydney, Sydney, NSW 2006, Australia
    Chem Soc Rev 42:8584-95. 2013
  3. ncbi 1912: a Titanic year for mass spectrometry
    Kevin M Downard
    School of Molecular Bioscience, The University of Sydney, Australia
    J Mass Spectrom 47:1034-9. 2012
  4. doi Homology-modelled structure of the βB2B3-crystallin heterodimer studied by ion mobility and radical probe MS
    Kevin M Downard
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    FEBS J 278:4044-54. 2011
  5. doi Impact of limited oxidation on protein ion mobility and structure of importance to footprinting by radical probe mass spectrometry
    Kevin M Downard
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Rapid Commun Mass Spectrom 26:226-30. 2012
  6. ncbi Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia
    J Virol Methods 165:178-85. 2010
  7. doi Ability of N-acetylcarnosine to protect lens crystallins from oxidation and oxidative damage by radical probe mass spectrometry (RP-MS)
    Ji Won Ha
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Rapid Commun Mass Spectrom 24:2900-8. 2010
  8. pmc FluShuffle and FluResort: new algorithms to identify reassorted strains of the influenza virus by mass spectrometry
    Aaron Tl Lun
    School of Molecular Bioscience G 08, The University of Sydney, Sydney, NSW, 2006, Australia
    BMC Bioinformatics 13:208. 2012
  9. ncbi Signature peptides of influenza nucleoprotein for the typing and subtyping of the virus by high resolution mass spectrometry
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences G 08, University of Sydney, Sydney, NSW 2006, Australia
    Analyst 134:2253-61. 2009
  10. pmc ETISEQ--an algorithm for automated elution time ion sequencing of concurrently fragmented peptides for mass spectrometry-based proteomics
    Jason W H Wong
    UNSW Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia
    BMC Bioinformatics 10:244. 2009

Collaborators

Detail Information

Publications43

  1. pmc Proteotyping to establish gene origin within reassortant influenza viruses
    Ji Won Ha
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 6:e15771. 2011
    ....
  2. ncbi Proteotyping for the rapid identification of influenza virus and other biopathogens
    Kevin M Downard
    School of Molecular Bioscience G 08, University of Sydney, Sydney, NSW 2006, Australia
    Chem Soc Rev 42:8584-95. 2013
    ..The broader application of the approach is further demonstrated here for the parainfluenza virus, a virus which can be life threatening to children and presents similar clinical symptoms to influenza. ..
  3. ncbi 1912: a Titanic year for mass spectrometry
    Kevin M Downard
    School of Molecular Bioscience, The University of Sydney, Australia
    J Mass Spectrom 47:1034-9. 2012
    ..This article, 100 years on, describes Taylor's work and its impact on safe ocean passage across the Atlantic...
  4. doi Homology-modelled structure of the βB2B3-crystallin heterodimer studied by ion mobility and radical probe MS
    Kevin M Downard
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    FEBS J 278:4044-54. 2011
    ..The βB2B3 heterodimer AD model provides the best representation of the heterodimer's structure overall following a consideration of both the ion mobility and radical probe MS data...
  5. doi Impact of limited oxidation on protein ion mobility and structure of importance to footprinting by radical probe mass spectrometry
    Kevin M Downard
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Rapid Commun Mass Spectrom 26:226-30. 2012
    ..Travelling wave ion mobility mass spectrometry has been used for the first time to confirm that limited oxidation does not have an impact on the global structure of proteins...
  6. ncbi Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia
    J Virol Methods 165:178-85. 2010
    ..The subsequent detection of the signature peptides of matrix M1 protein within proteolytic digests of type A and type B human and avian strains has been demonstrated...
  7. doi Ability of N-acetylcarnosine to protect lens crystallins from oxidation and oxidative damage by radical probe mass spectrometry (RP-MS)
    Ji Won Ha
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Rapid Commun Mass Spectrom 24:2900-8. 2010
    ....
  8. pmc FluShuffle and FluResort: new algorithms to identify reassorted strains of the influenza virus by mass spectrometry
    Aaron Tl Lun
    School of Molecular Bioscience G 08, The University of Sydney, Sydney, NSW, 2006, Australia
    BMC Bioinformatics 13:208. 2012
    ..These algorithms enable reassorted influenza, and other, viruses to be rapidly identified to allow prevention strategies and treatments to be more efficiently implemented...
  9. ncbi Signature peptides of influenza nucleoprotein for the typing and subtyping of the virus by high resolution mass spectrometry
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences G 08, University of Sydney, Sydney, NSW 2006, Australia
    Analyst 134:2253-61. 2009
    ..The application of the approach is illustrated for both type A H1N1 and H3N2, and type B strains of human influenza virus...
  10. pmc ETISEQ--an algorithm for automated elution time ion sequencing of concurrently fragmented peptides for mass spectrometry-based proteomics
    Jason W H Wong
    UNSW Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia
    BMC Bioinformatics 10:244. 2009
    ..Nevertheless, concurrent peptide fragmentation data acquisition remains to be widely adopted due to the lack of published algorithms designed specifically to process or interpret such data acquired on any mass spectrometer...
  11. doi Mass spectrometry analysis of the influenza virus
    Kevin M Downard
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, NSW, Australia
    Mass Spectrom Rev 28:35-49. 2009
    ..This article was published online 10 September 2008. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 7 November 2008.]..
  12. doi Kinetics of antigen-antibody interactions employing a MALDI mass spectrometry immunoassay
    Bethny Morrissey
    School of Molecular and Microbial Biosciences, The University of Sydney, Australia
    Anal Chem 80:7720-6. 2008
    ..Studies of modified peptide variants and synthetic variants of HA peptide 119-125 has enabled important contact residues to be identified whose accessibilities in the native protein are in accord with the mass spectrometry results...
  13. pmc FluTyper-an algorithm for automated typing and subtyping of the influenza virus from high resolution mass spectral data
    Jason W H Wong
    Prince of Wales Clinical School and Lowy Cancer Research Centre, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia
    BMC Bioinformatics 11:266. 2010
    ..This analysis has, to date, required the manual inspection of mass spectra of whole virus and antigen digests...
  14. ncbi Subtyping of influenza neuraminidase using mass spectrometry
    An P Nguyen
    School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
    Analyst 138:1787-93. 2013
    ..This should help facilitate a more rapid response in the event of a local epidemic or global pandemic...
  15. doi Mass trees: a new phylogenetic approach and algorithm to chart evolutionary history with mass spectrometry
    Aaron T L Lun
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    Anal Chem 85:5475-82. 2013
    ..Given that the mass map data can be generated more rapidly than gene sequences, even when next generation parallel sequencing is employed, mass trees offer new opportunities and advantages for phylogenetic analysis...
  16. ncbi Rapid typing and subtyping of vaccine strains of the influenza virus with high resolution mass spectrometry
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences G 08, University of Sydney, NSW, Australia
    Eur J Mass Spectrom (Chichester, Eng) 16:321-9. 2010
    ..The approach is demonstrated for two type A strains and one type B strain of human influenza viruses present in recommended seasonal vaccines in the northern and southern hemispheres from 2007 through 2010...
  17. doi Subtyping of the influenza virus by high resolution mass spectrometry
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Anal Chem 81:3500-6. 2009
    ..The approach represents a more rapid and direct approach with which to type and subtype the virus that is of critical need to prepare strategies and treatments in the event of a local epidemic or global pandemic...
  18. ncbi Inhibition of influenza hemagglutinin with the antiviral inhibitor arbidol using a proteomics based approach and mass spectrometry
    Zainab H Nasser
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Antiviral Res 100:399-406. 2013
    ..The combined studies support the recognized potential of arbidol as an effective and targeted antiviral agent against the influenza virus. ..
  19. ncbi Binding of a natural anthocyanin inhibitor to influenza neuraminidase by mass spectrometry
    Kavya Swaminathan
    School of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia
    Anal Bioanal Chem 405:6563-72. 2013
    ....
  20. ncbi Onset of oxidative damage in alpha-crystallin by radical probe mass spectrometry
    Wai Kei Shum
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Anal Biochem 344:247-56. 2005
    ..The results are discussed in the context of available experimental and homology-modeled theoretical structures for the subunits of alpha-crystallin...
  21. ncbi Hydroxyl radical probe of the calmodulin-melittin complex interface by electrospray ionization mass spectrometry
    Jason W H Wong
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia
    J Am Soc Mass Spectrom 16:225-33. 2005
    ....
  22. ncbi Evolution of influenza neuraminidase and the detection of antiviral resistant strains using mass trees
    Kavya Swaminathan
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales NSW 2006, Australia
    Anal Chem 86:629-37. 2014
    ..This new computational approach is expected to find wider application for evolutionary studies of organisms more generally. ..
  23. ncbi Fingerprinting a killer: surveillance of the influenza virus by mass spectrometry
    Kevin M Downard
    School of Molecular and Microbial Biosciences G 08, The University of Sydney, Sydney, NSW 2006, Australia
    Analyst 132:611-4. 2007
    ....
  24. ncbi Rapid differentiation of seasonal and pandemic H1N1 influenza through proteotyping of viral neuraminidase with mass spectrometry
    Alexander B Schwahn
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    Anal Chem 82:4584-90. 2010
    ..The detection of these peptides can enable the origins of the neuraminidase gene to be monitored in the case of reassorted strains...
  25. ncbi Anti-viral inhibitor binding to influenza neuraminidase by MALDI mass spectrometry
    Kavya Swaminathan
    School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
    Anal Chem 84:3725-30. 2012
    ..The ability to identify peptides within the active sites of proteins and likely binding residues provides both a rapid and relatively high throughput approach with which to screen protein-drug interactions by MALDI mass spectrometry...
  26. ncbi Interaction between alpha and upsilon-crystallin, common to the eye of the Australian platypus, by radical probe mass spectrometry
    Samah Issa
    School of Molecular and Microbial Biosciences, University of Sydney, Australia
    J Mass Spectrom 41:1298-303. 2006
    ..Across each domain, a delay in the onset of oxidative damage is observed as the time of exposure to radicals is increased. The results are discussed in the context of the structure of the porcine homologue of upsilon-crystallin...
  27. doi Evolution of H5N1 influenza virus through proteotyping of hemagglutinin with high resolution mass spectrometry
    Ji Won Ha
    School of Molecular Bioscience G 08, University of Sydney, Sydney, NSW 2006, Australia
    Analyst 136:3259-67. 2011
    ..Nevertheless, several period-specific signature peptides were identified that enable strains associated with the 1997 H5N1 pandemic to be rapidly differentiated from those in circulation across the subsequent decade...
  28. doi Proteotyping to establish the lineage of type A H1N1 and type B human influenza virus
    Alexander B Schwahn
    School of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia
    J Virol Methods 171:117-22. 2011
    ..This proteotyping approach complements conventional PCR approaches and should aid in the monitoring of the evolution of the influenza virus in both humans and animals...
  29. doi Antigenicity of a type A influenza virus through comparison of hemagglutination inhibition and mass spectrometry immunoassays
    Alexander B Schwahn
    School of Molecular and Microbial Biosciences, University of Sydney, Australia
    J Immunoassay Immunochem 30:245-61. 2009
    ..Both immunoassays showed no binding of a monoclonal antibody that recognizes the hemagglutinin antigen of type B strains...
  30. ncbi COMPLX: a computer algorithm for the detection of protein-ligand and other macromolecular complexes in mass spectra
    Jason W H Wong
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Mass Spectrom 38:573-81. 2003
    ..Thus the program will be of value in a future goal of proteomics, where mass spectrometry already plays a central role, for the direct analysis of protein and other associations within biological extracts...
  31. ncbi On-plate deposition of oxidized proteins to facilitate protein footprinting studies by radical probe mass spectrometry
    Simin D Maleknia
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Rapid Commun Mass Spectrom 26:2311-8. 2012
    ..The on-plate deposition of lysozyme oxidized at electrospray needle voltages of 6 and 9 kV were carried out to demonstrate conditions suitable for footprinting experiments as well as those that induce the onset of protein damage...
  32. ncbi Strategy for identifying protein-protein interactions of gel-separated proteins and complexes by mass spectrometry
    Kenneth Mackun
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, New South Wales 2006, Australia
    Anal Biochem 318:60-70. 2003
    ..The strategy can be easily adapted to achieve high sample throughput and automation using gel-excision robotics and provides a means to study protein interactions in complex biological mixtures and extracts...
  33. ncbi Ions of the interactome: the role of MS in the study of protein interactions in proteomics and structural biology
    Kevin M Downard
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Proteomics 6:5374-84. 2006
    ..These new and emerging applications, which further demonstrate the power of MS, continue to ensure that the mass spectrometer will remain at the heart of discoveries in proteomics in the foreseeable future...
  34. ncbi A computer algorithm for the identification of protein interactions from the spectra of masses (PRISM)
    Joshua W K Ho
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, NSW 2006, Australia
    J Am Soc Mass Spectrom 18:563-6. 2007
    ..The algorithm has general utility for the comparative analysis of differences within any two mass spectra of any type and is easily implemented using a simple, intuitive graphical user interface (GUI)...
  35. ncbi PROXIMO--a new docking algorithm to model protein complexes using data from radical probe mass spectrometry (RP-MS)
    Sebastien K Gerega
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, NSW 2006, Australia
    Bioinformatics 22:1702-9. 2006
    ..The former is in close agreement with the high-resolution experimental structure available...
  36. ncbi Substituent effects on the binding of natural product anthocyanidin inhibitors to influenza neuraminidase with mass spectrometry
    Kavya Swaminathan
    Marie Bashir Institute for Infectious Diseases and Biosecurity, Molecular Bioscience Building G08, University of Sydney, Sydney, New South Wales 2006, Australia
    Anal Chim Acta 828:61-9. 2014
    ..It has broader application for the screening of other protein interactions more generally with reasonable high-throughput. ..
  37. doi An immunoproteomics approach to screen the antigenicity of the influenza virus
    Kevin M Downard
    School of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia
    Methods Mol Biol 1061:141-53. 2013
    ..The approach is anticipated to have a greater role in the future surveillance of the virus and should also aid in the development of immunogenic peptide constructs as alternatives to whole virus for vaccination. ..
  38. ncbi A proteomics approach to survey the antigenicity of the influenza virus by mass spectrometry
    Bethny Morrissey
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia
    Proteomics 6:2034-41. 2006
    ..The approach has broad applicability for the analysis of a wide array of protein complexes with identification of the interaction interface in a single step with high-sample throughput and at low sample levels...
  39. doi Proteotyping of the parainfluenza virus with high-resolution mass spectrometry
    An P Nguyen
    School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
    Anal Chem 85:1097-105. 2013
    ....
  40. ncbi Antigenic characterisation of H3N2 subtypes of the influenza virus by mass spectrometry
    Bethny Morrissey
    School of Molecular and Microbial Biosciences, The University of Sydney, Australia
    J Virol Methods 145:106-14. 2007
    ..Comparative rates of antibody binding between two of the antigenic peptides have also been reported...
  41. ncbi Performance of the computer algorithm COMPLX for the detection of protein complexes in the mass spectra of simulated biological mixtures
    Jason W H Wong
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia
    J Mass Spectrom 40:1187-96. 2005
    ....
  42. ncbi Advances in radical probe mass spectrometry for protein footprinting in chemical biology applications
    Simin D Maleknia
    School of Civil and Environmental Engineering, University of New South Wales, Sydney, Australia
    Chem Soc Rev 43:3244-58. 2014
    ..The accessibility of the RP-MS technology, by means of a modified electrospray ionization source, enables the approach to be implemented in many laboratories to address a wide range of applications in chemical biology. ..
  43. ncbi Study of the ribonuclease-S-protein-peptide complex using a radical probe and electrospray ionization mass spectrometry
    Jason W H Wong
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia
    Anal Chem 75:1557-63. 2003
    ..The results, for the first time, demonstrate the use of radicals generated by an electrical discharge to study protein complexes...