Pavel Dolezal

Summary

Publications

  1. pmc Live imaging of mitosomes and hydrogenosomes by HaloTag technology
    Eva Martincová
    Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
    PLoS ONE 7:e36314. 2012
  2. pmc The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica
    Pavel Dolezal
    Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia
    PLoS Pathog 6:e1000812. 2010
  3. pmc The protein import channel in the outer mitosomal membrane of Giardia intestinalis
    Michael J Dagley
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia
    Mol Biol Evol 26:1941-7. 2009
  4. pmc Frataxin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis
    Pavel Dolezal
    Department of Parasitology, Charles University, Faculty of Science, Vinicna 7, 128 44 Prague, Czech Republic
    Eukaryot Cell 6:1431-8. 2007
  5. pmc The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis
    Petr Rada
    Department of Parasitology, Charles University in Prague, Faculty of Science, Prague, Czech Republic
    PLoS ONE 6:e24428. 2011
  6. pmc The monothiol single-domain glutaredoxin is conserved in the highly reduced mitochondria of Giardia intestinalis
    Petr Rada
    Charles University in Prague, Faculty of Science, Department of Parasitology, Vinicna 7, 128 44 Prague, Czech Republic
    Eukaryot Cell 8:1584-91. 2009
  7. ncbi request reprint Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex I
    Ivan Hrdy
    Department of Parasitology, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
    Nature 432:618-22. 2004
  8. pmc The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis
    Petr L Jedelský
    Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic
    PLoS ONE 6:e17285. 2011
  9. doi request reprint Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis
    Robert Sutak
    Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague, Czech Republic
    FEBS J 279:2768-80. 2012
  10. doi request reprint A small Tim homohexamer in the relict mitochondrion of Cryptosporidium
    Felicity Alcock
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
    Mol Biol Evol 29:113-22. 2012

Collaborators

Detail Information

Publications22

  1. pmc Live imaging of mitosomes and hydrogenosomes by HaloTag technology
    Eva Martincová
    Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
    PLoS ONE 7:e36314. 2012
    ..With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes...
  2. pmc The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica
    Pavel Dolezal
    Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia
    PLoS Pathog 6:e1000812. 2010
    ..histolytica...
  3. pmc The protein import channel in the outer mitosomal membrane of Giardia intestinalis
    Michael J Dagley
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia
    Mol Biol Evol 26:1941-7. 2009
    ..As Tom40 was derived in the development of mitochondria to serve as the protein import channel in the outer membrane, its presence in Giardia evidences the mitochondrial ancestry of mitosomes...
  4. pmc Frataxin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis
    Pavel Dolezal
    Department of Parasitology, Charles University, Faculty of Science, Vinicna 7, 128 44 Prague, Czech Republic
    Eukaryot Cell 6:1431-8. 2007
    ..The discovery of conserved iron-metabolizing pathways in mitochondria and hydrogenosomes provides additional evidence not only of their common evolutionary history, but also of the fundamental importance of this pathway for eukaryotes...
  5. pmc The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis
    Petr Rada
    Department of Parasitology, Charles University in Prague, Faculty of Science, Prague, Czech Republic
    PLoS ONE 6:e24428. 2011
    ....
  6. pmc The monothiol single-domain glutaredoxin is conserved in the highly reduced mitochondria of Giardia intestinalis
    Petr Rada
    Charles University in Prague, Faculty of Science, Department of Parasitology, Vinicna 7, 128 44 Prague, Czech Republic
    Eukaryot Cell 8:1584-91. 2009
    ..intestinalis thus completes the mitosomal FeS cluster biosynthetic pathway and provides further evidence for the mitochondrial origin of these organelles...
  7. ncbi request reprint Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex I
    Ivan Hrdy
    Department of Parasitology, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
    Nature 432:618-22. 2004
    ..Recruitment of complex I subunits into a H2-producing pathway provides evidence that mitochondria and hydrogenosomes are aerobic and anaerobic homologues of the same endosymbiotically derived organelle...
  8. pmc The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis
    Petr L Jedelský
    Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic
    PLoS ONE 6:e17285. 2011
    ..The small proteome of the Giardia mitosome reflects the reduction in mitochondrial metabolism, which is limited to the FeS cluster assembly pathway, and a simplicity in the protein import pathway required for organelle biogenesis...
  9. doi request reprint Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis
    Robert Sutak
    Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague, Czech Republic
    FEBS J 279:2768-80. 2012
    ..vaginalis to modulate the metabolic fluxes indicate efficacious metabolic responsiveness that could be advantageous for rapid adaptation of the parasite to changes in the host environment...
  10. doi request reprint A small Tim homohexamer in the relict mitochondrion of Cryptosporidium
    Felicity Alcock
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
    Mol Biol Evol 29:113-22. 2012
    ..We show that the atypical CpTimS exists as a relatively unstable homohexamer, shedding light both on the early evolution of the small Tim protein family and on small Tim hexamer formation in contemporary eukaryotes...
  11. pmc Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana
    Janette Tong
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
    Mol Biol Evol 28:1581-91. 2011
    ..Reclinomonas americana provides direct evidence that bacterial protein transport pathways were retained, alongside the evolving mitochondrial protein import machinery, shedding new light on the process of mitochondrial evolution...
  12. pmc Protein secretion and outer membrane assembly in Alphaproteobacteria
    Xenia Gatsos
    Department of Biochemistry and Molecular Biology, University of Melbourne, Melbourne, Australia
    FEMS Microbiol Rev 32:995-1009. 2008
    ....
  13. doi request reprint Using hidden markov models to discover new protein transport machines
    Vladimir A Likic
    Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria, Australia
    Methods Mol Biol 619:271-84. 2010
    ..The Tom40 sequence could not be found with BLAST-based searches, but a hidden Markov model search identified a likely candidate to form the protein import pore in the outer mitosomal membrane in E. histolytica...
  14. pmc The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system
    Zuzana Zubacova
    Charles University in Prague, Faculty of Science, Department of Parasitology, Prague, Czech Republic
    PLoS ONE 8:e55417. 2013
    ..Glycine cleavage system is at the moment the only experimentally localized pathway in the mitochondrial derivate of Trimastix pyriformis...
  15. ncbi request reprint Evolution of the molecular machines for protein import into mitochondria
    Pavel Dolezal
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville 3010, Australia
    Science 313:314-8. 2006
    ..The presence of similar translocase subunits in all eukaryotic genomes sequenced to date suggests that all eukaryotes can be considered descendants of a single ancestor species that carried an ancestral "protomitochondria."..
  16. pmc Giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting
    Pavel Dolezal
    Department of Parasitology, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
    Proc Natl Acad Sci U S A 102:10924-9. 2005
    ..A shared mode of protein targeting supports the hypothesis that mitosomes, hydrogenosomes, and mitochondria represent different forms of the same fundamental organelle having evolved under distinct selection pressures...
  17. pmc Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis
    Robert Sutak
    Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
    Proc Natl Acad Sci U S A 101:10368-73. 2004
    ..Our results indicate that the biosynthesis of FeS proteins is performed by a homologous system in mitochondriate and amitochondriate eukaryotes and that this process is inherited from the proteobacterial ancestor of mitochondria...
  18. ncbi request reprint Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins
    Pavel Dolezal
    Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 12844, Prague 2, Czech Republic
    Gene 329:81-92. 2004
    ..vaginalis. The presence of both prokaryotic and eukaryotic type of malic enzyme in different compartments of a single eukaryotic cell appears to be unique in nature...
  19. ncbi request reprint Conserved motifs reveal details of ancestry and structure in the small TIM chaperones of the mitochondrial intermembrane space
    Ian E Gentle
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia
    Mol Biol Evol 24:1149-60. 2007
    ..The conserved features in the Tim10 and Tim13 subunits provide distinct binding surfaces to accommodate the broad range of substrate proteins delivered to the mitochondrial inner and outer membranes...
  20. ncbi request reprint Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomes
    Pavel Suchan
    Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 44, Prague 2, Czech Republic
    Microbiology 149:1911-21. 2003
    ..Analysis of Tt. foetus hydrogenosomes showed at least nine iron-binding compounds, which were absent in metronidazole-resistant mutants. The major iron-binding compound was identified as [2Fe-2S] ferredoxin of the adrenodoxin type...
  21. pmc Legionella pneumophila secretes a mitochondrial carrier protein during infection
    Pavel Dolezal
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
    PLoS Pathog 8:e1002459. 2012
    ..A hidden Markov model revealed further MCF proteins in the intracellular pathogens, Legionella longbeachae and Neorickettsia sennetsu, thereby challenging the notion that MCF proteins exist exclusively in eukaryotic organisms...
  22. ncbi request reprint Tom40 is likely common to all mitochondria
    Vojtech Zársky
    Charles University in Prague, Faculty of Science, Department of Parasitology, Vinicna 7, 128 44, Prague 2, Czech Republic
    Curr Biol 22:R479-81. 2012
    ..Surprisingly, our results clearly refute the link between ATOM and bacterial Omp85-like proteins. Moreover, we propose that ATOM is, in fact, a divergent form of the 'classical' Tom40...