Cassian Sitaru

Summary

Affiliation: Disaster Mental Health Institute

Publications

  1. ncbi request reprint Enzyme-linked immunosorbent assay using multimers of the 16th non-collagenous domain of the BP180 antigen for sensitive and specific detection of pemphigoid autoantibodies
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, Lubeck, Germany
    Exp Dermatol 16:770-7. 2007
  2. ncbi request reprint Experimental models of epidermolysis bullosa acquisita
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    Exp Dermatol 16:520-31. 2007
  3. ncbi request reprint Mechanisms of blister induction by autoantibodies
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    Exp Dermatol 14:861-75. 2005
  4. pmc The relevance of the IgG subclass of autoantibodies for blister induction in autoimmune bullous skin diseases
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Arch Dermatol Res 299:1-8. 2007
  5. pmc Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Clin Invest 115:870-8. 2005
  6. ncbi request reprint Induction of complement-fixing autoantibodies against type VII collagen results in subepidermal blistering in mice
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Immunol 177:3461-8. 2006
  7. doi request reprint Bullous pemphigoid: a prototypical antibody-mediated organ-specific autoimmune disease
    Cassian Sitaru
    Department of Dermatology, University Medical Center Freiburg, Freiburg, Germany
    J Invest Dermatol 129:822-4. 2009
  8. ncbi request reprint Granulocyte-derived elastase and gelatinase B are required for dermal-epidermal separation induced by autoantibodies from patients with epidermolysis bullosa acquisita and bullous pemphigoid
    Iakov Shimanovich
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    J Pathol 204:519-27. 2004
  9. ncbi request reprint IgG4 autoantibodies induce dermal-epidermal separation
    Sidonia Mihai
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Cell Mol Med 11:1117-28. 2007
  10. ncbi request reprint Localized linear IgA disease induced by ampicillin/sulbactam
    Iakov Shimanovich
    Department of Dermatology, University of Wurzburg, Germany
    J Am Acad Dermatol 51:95-8. 2004

Collaborators

Detail Information

Publications54

  1. ncbi request reprint Enzyme-linked immunosorbent assay using multimers of the 16th non-collagenous domain of the BP180 antigen for sensitive and specific detection of pemphigoid autoantibodies
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, Lubeck, Germany
    Exp Dermatol 16:770-7. 2007
    ..In conclusion, the use of tetrameric NC16A in ELISA results in a sensitive and specific tool for diagnosis and monitoring of BP and PG...
  2. ncbi request reprint Experimental models of epidermolysis bullosa acquisita
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    Exp Dermatol 16:520-31. 2007
    ..The new mechanistic insights gained from investigating EBA pathogenesis will facilitate the design of immunomodulatory interventions for this and other pathogenetically related organ-specific, antibody-dependent autoimmune diseases...
  3. ncbi request reprint Mechanisms of blister induction by autoantibodies
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    Exp Dermatol 14:861-75. 2005
    ..Understanding the mechanisms by which autoantibodies induce blisters should facilitate the development of more specific therapeutic strategies of autoimmune blistering diseases...
  4. pmc The relevance of the IgG subclass of autoantibodies for blister induction in autoimmune bullous skin diseases
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Arch Dermatol Res 299:1-8. 2007
    ..Characterization of the pathogenically relevant subclass(es) of autoantibodies not only provides mechanistic insights, but should greatly facilitate the development of improved therapeutic modalities of autoimmune blistering diseases...
  5. pmc Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Clin Invest 115:870-8. 2005
    ..This animal model for EBA should facilitate further dissection of the pathogenesis of this disease and development of new therapeutic strategies...
  6. ncbi request reprint Induction of complement-fixing autoantibodies against type VII collagen results in subepidermal blistering in mice
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Immunol 177:3461-8. 2006
    ..This robust experimental system should greatly facilitate further studies on the pathogenesis of EBA and the development of novel immunomodulatory therapies for this and other autoimmune diseases...
  7. doi request reprint Bullous pemphigoid: a prototypical antibody-mediated organ-specific autoimmune disease
    Cassian Sitaru
    Department of Dermatology, University Medical Center Freiburg, Freiburg, Germany
    J Invest Dermatol 129:822-4. 2009
    ..These novel findings shed new light on additional mechanisms of blister formation in pemphigoid diseases and open the way for further informative studies...
  8. ncbi request reprint Granulocyte-derived elastase and gelatinase B are required for dermal-epidermal separation induced by autoantibodies from patients with epidermolysis bullosa acquisita and bullous pemphigoid
    Iakov Shimanovich
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    J Pathol 204:519-27. 2004
    ..These findings strongly suggest that elastase and gelatinase B are essential for granulocyte-mediated proteolysis resulting in dermal-epidermal separation in EBA and BP patients' skin...
  9. ncbi request reprint IgG4 autoantibodies induce dermal-epidermal separation
    Sidonia Mihai
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Cell Mol Med 11:1117-28. 2007
    ..Moreover, for the first time, we clearly demonstrate that BP IgG4 autoantibodies have the capacity to induce leucocyte-dependent tissue damage...
  10. ncbi request reprint Localized linear IgA disease induced by ampicillin/sulbactam
    Iakov Shimanovich
    Department of Dermatology, University of Wurzburg, Germany
    J Am Acad Dermatol 51:95-8. 2004
    ..Discontinuation of the antibiotics resulted in a rapid resolution of the skin lesions. This is a most unusual case of localized drug-induced linear IgA disease...
  11. pmc Autoantibodies to type VII collagen mediate Fcgamma-dependent neutrophil activation and induce dermal-epidermal separation in cryosections of human skin
    Cassian Sitaru
    Department of Dermatology, University of Wurzburg, Wurzburg, Germany
    Am J Pathol 161:301-11. 2002
    ..We demonstrate the capacity of autoantibodies to type VII collagen to trigger an Fcgamma-dependent inflammation leading to split formation in cryosections of human skin...
  12. doi request reprint T cells are required for the production of blister-inducing autoantibodies in experimental epidermolysis bullosa acquisita
    Ana Gabriela Sitaru
    Institute for Medical Microbiology and Hygiene, University of Lubeck, Lubeck, Germany
    J Immunol 184:1596-603. 2010
    ....
  13. ncbi request reprint Localisation of bullous pemphigoid antigen 180 (BP180) in cultured human keratinocytes: functionally relevant modification by calcium
    Enno Schmidt
    Department of Dermatology, University of Wurzburg, Josef Schneider Str 2, 97080 Wurzburg, Germany
    Arch Dermatol Res 298:283-90. 2006
    ..Our data indicate that the Ca(2+)-modulated expression of BP180 is functionally relevant. This finding sheds further light on the complex pathomechanism in blister formation of BP180-related autoimmune blistering skin diseases...
  14. pmc The flavonoid luteolin inhibits Fcγ-dependent respiratory burst in granulocytes, but not skin blistering in a new model of pemphigoid in adult mice
    Eva Oswald
    Allergy Research Group, Department of Dermatology, University Freiburg Medical Center, Freiburg, Germany
    PLoS ONE 7:e31066. 2012
    ..These studies establish a robust animal model that will be a useful tool for dissecting the mechanisms of blister formation and will facilitate the development of more effective therapeutic strategies for managing pemphigoid diseases...
  15. doi request reprint Cross-reactivity of autoantibodies from patients with epidermolysis bullosa acquisita with murine collagen VII
    Kinga Csorba
    Department of Dermatology, University of Freiburg, Freiburg, Germany
    Cell Mol Life Sci 67:1343-51. 2010
    ..These findings should greatly facilitate the development of improved disease models and novel therapeutic strategies...
  16. ncbi request reprint Scarring autoimmune bullous disease in a Ugandan patient with autoantibodies to BP180, BP230, and laminin 5
    Grace K Mulyowa
    Department of Dermatology, University of Wurzburg, Wurzburg, Germany
    J Am Acad Dermatol 54:S43-6. 2006
    ..Here, we describe a scarring mucocutaneous variant of an autoimmune blistering skin disorder that extends the current clinical and immunopathologic spectrum of this group of diseases...
  17. ncbi request reprint Immunoblotting and enzyme-linked immunosorbent assay for the diagnosis of pemphigoid gestationis
    Cassian Sitaru
    Department of Dermatology, University of Wurzburg, Wurzburg, Germany
    Obstet Gynecol 103:757-63. 2004
    ....
  18. ncbi request reprint Generation and characterization of monoclonal antibodies against the intracellular domain of hemidesmosomal type XVII collagen
    Florina Olaru
    Department of Dermatology, University of Lubeck, Lubeck, Romania
    Hybridoma (Larchmt) 25:158-62. 2006
    ..Possible applications of this new MAb include antigen mapping in patients with hereditary epidermolysis bullosa and immunoaffinity purification of cell-derived type XVII collagen...
  19. doi request reprint Induction of experimental epidermolysis bullosa acquisita by immunization with murine collagen VII
    Alina Sesarman
    Department of Dermatology, University of Freiburg, Freiburg, Germany
    Methods Mol Biol 961:371-87. 2013
    ....
  20. doi request reprint Pathogenicity of IgG subclass autoantibodies to type VII collagen: induction of dermal-epidermal separation
    Andreas Recke
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    J Autoimmun 34:435-44. 2010
    ..e. the generation of subclass-specific adsorbers, used for extracorporal immunoapheresis, or the shifting of the autoimmune response to production of non-pathogenic autoantibodies...
  21. doi request reprint Transition from pemphigus foliaceus to bullous pemphigoid: intermolecular B-cell epitope spreading without IgG subclass shifting
    Andreas Recke
    Department of Dermatology at University of Lübeck, Ratzeburger Allee 160, Lubeck 23538, Germany
    J Am Acad Dermatol 61:333-6. 2009
    ..Of interest, although the autoimmune response clearly shifted with regard to the target antigens, the patient's autoantibodies against desmosomal and hemidesmosomal components showed the same IgG subclass distribution...
  22. doi request reprint Why human pemphigoid autoantibodies do not trigger disease by the passive transfer into mice?
    Alina Sesarman
    Department of Dermatology, University of Freiburg, Freiburg, Germany
    Immunol Lett 143:92-100. 2012
    ..These results indicate that for disease models using the passive transfer of patient autoantibodies, their interaction with the innate factors of the host should be optimized to match the human situation...
  23. ncbi request reprint The autoantigen of anti-p200 pemphigoid is an acidic noncollagenous N-linked glycoprotein of the cutaneous basement membrane
    Iakov Shimanovich
    Department of Dermatology, University of Wurzburg, Wurzburg, Germany
    J Invest Dermatol 121:1402-8. 2003
    ..4 to 5.6. Six different p200-specific sera recognized an identical protein spot of two-dimensionally separated dermal extracts, confirming that patients with this novel autoimmune disease indeed form a single pathobiochemical entity...
  24. ncbi request reprint The alternative pathway of complement activation is critical for blister induction in experimental epidermolysis bullosa acquisita
    Sidonia Mihai
    Department of Dermatology, University of Lubeck, Lubeck, Germany, and Department of Pediatrics, Laboratory of Developmental Immunology, Massachusetts General Hospital, Boston 02115, USA
    J Immunol 178:6514-21. 2007
    ....
  25. pmc Prevalence of collagen VII-specific autoantibodies in patients with autoimmune and inflammatory diseases
    Emilia Licarete
    Department of Dermatology, University of Freiburg, Hauptstr, 7, Freiburg 79104, Germany
    BMC Immunol 13:16. 2012
    ..The aim of our present study was to develop an accurate immunoassay for assessing the presence of autoantibodies against collagen VII in large cohorts of patients and healthy donors...
  26. doi request reprint Neonatal Fc receptor deficiency protects from tissue injury in experimental epidermolysis bullosa acquisita
    Alina Sesarman
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Mol Med (Berl) 86:951-9. 2008
    ..Therefore, modulation of FcRn function using inhibitors may reduce pathogenic IgG levels, offering therapeutic benefit in patients with antibody-mediated diseases...
  27. ncbi request reprint Immunoadsorption against two distinct epitopes on human type XVII collagen abolishes dermal-epidermal separation induced in vitro by autoantibodies from pemphigoid gestationis patients
    Josep E Herrero-Gonzalez
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    Eur J Immunol 36:1039-48. 2006
    ..This study identifies the epitopes relevant for blister induction in PG and should facilitate the development of an antigen-specific immunoadsorption therapy for this disease...
  28. doi request reprint The neonatal Fc receptor as therapeutic target in IgG-mediated autoimmune diseases
    Alina Sesarman
    Department of Dermatology, University of Freiburg, Hauptstrasse 7, Freiburg, Germany
    Cell Mol Life Sci 67:2533-50. 2010
    ....
  29. doi request reprint Ectodomain shedding generates Neoepitopes on collagen XVII, the major autoantigen for bullous pemphigoid
    Wataru Nishie
    Department of Dermatology, University Medical Center Freiburg, Freiburg, Germany
    J Immunol 185:4938-47. 2010
    ..Taken together, these data demonstrate that physiological shedding of collagen XVII generates neoepitopes, which may serve as a target of blister-inducing autoantibodies...
  30. ncbi request reprint Subepidermal blistering disease with autoantibodies to both the p200 autoantigen and the alpha3 chain of laminin 5
    Iakov Shimanovich
    Department of Dermatology, University of Lubeck, Germany
    J Am Acad Dermatol 52:S90-2. 2005
    ..The unusual clinical and immunologic profile of this case suggests that molecular specificity of circulating autoantibodies affects the clinical presentation of autoimmune subepidermal blistering skin diseases...
  31. pmc Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases
    Kinga Csorba
    Department of Dermatology, University of Freiburg, Hauptstrasse 7, 79104, Freiburg, Germany
    Orphanet J Rare Dis 6:31. 2011
    ..Therefore, the aim of the present study was to develop an ELISA to detect IgA autoantibodies against collagen XVII in the sera of patients with pemphigoids...
  32. ncbi request reprint Autoantibodies to bullous pemphigoid antigen 180 induce dermal-epidermal separation in cryosections of human skin
    Cassian Sitaru
    Department of Dermatology, University of Wurzburg, Wurzburg, Germany
    J Invest Dermatol 118:664-71. 2002
    ..Our in vitro results support the idea that autoantibodies to bullous pemphigoid 180 from patients with bullous pemphigoid are of pathogenic relevance...
  33. ncbi request reprint Immunopathology and molecular diagnosis of autoimmune bullous diseases
    Sidonia Mihai
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Cell Mol Med 11:462-81. 2007
    ..This review article describes the immunopathological features of autoimmune bullous diseases and the immunological and molecular tests used for their diagnosis and monitoring...
  34. ncbi request reprint Autoreactive T cell responses in pemphigus and pemphigoid
    Gertie J Oostingh
    Department of Dermatology, University of Wurzburg, Josef Schneider Str 2, 97080 Wurzburg, Germany
    Autoimmun Rev 1:267-72. 2002
    ..In this review, the current knowledge on T cell involvement in pemphigus and pemphigoid is summarised...
  35. ncbi request reprint Subacute prurigo variant of bullous pemphigoid: autoantibodies show the same specificity compared with classic bullous pemphigoid
    Enno Schmidt
    Department of Dermatology, University of Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    J Am Acad Dermatol 47:133-6. 2002
    ..Our findings suggest that the subacute prurigo form of BP is a true variant of BP...
  36. doi request reprint IgG antibodies against immunodominant C-terminal epitopes of BP230 do not induce skin blistering in mice
    Vasile Feldrihan
    Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Department of Dermatology, University of Freiburg, Freiburg, Germany
    Hum Immunol 75:354-63. 2014
    ....
  37. doi request reprint Ex vivo pathogenicity of anti-laminin γ1 autoantibodies
    Florina Florea
    Department of Dermatology, University Medical Center Freiburg, Freiburg, Germany
    Am J Pathol 184:494-506. 2014
    ..Our results provide mechanistic insights into the tissue damage associated with laminin autoimmunity and could facilitate development of appropriate diagnostic tools and therapeutic strategies. ..
  38. doi request reprint Molecular dermatology comes of age
    Cristina Has
    Department of Molecular Dermatology, University of Freiburg, Freiburg, Germany
    Methods Mol Biol 961:1-16. 2013
    ..This article highlights several major developments in molecular experimental and clinical dermatology...
  39. ncbi request reprint A highly sensitive and simple assay for the detection of circulating autoantibodies against full-length bullous pemphigoid antigen 180
    Enno Schmidt
    Department of Biotechnology, Biocenter, University of Wurzburg, Wurzburg, Germany
    J Autoimmun 18:299-309. 2002
    ..Thus, this assay meets the demands of a simple and effective diagnostic tool for detecting circulating antibodies against FL-BP180 and may also be used in laboratories without access to molecular biological technology...
  40. ncbi request reprint Memory B cells specific for the NC16A domain of the 180 kDa bullous pemphigoid autoantigen can be detected in peripheral blood of bullous pemphigoid patients and induced in vitro to synthesize autoantibodies
    Heike Leyendeckers
    Miltenyi Biotec GmbH, Bergisch Gladbach, Germany
    J Invest Dermatol 120:372-8. 2003
    ....
  41. ncbi request reprint The 97-kDa (LABD97) and 120-kDa (LAD-1) fragments of bullous pemphigoid antigen 180/type XVII collagen have different N-termini
    Yoshiaki Hirako
    J Invest Dermatol 121:1554-6. 2003
  42. ncbi request reprint Development of an ELISA for the detection of autoantibodies to BP230
    Arno Kromminga
    Institute for Immunology, Clinical Pathology, and Molecular Medicine IPM, Hamburg, Germany
    Clin Immunol 111:146-52. 2004
    ..This ELISA provides a useful tool for the detection of autoantibodies to BP230 in BP and other diseases associated with an autoimmune response to this protein...
  43. ncbi request reprint [Bullous autoimmune dermatoses (I): Pathogenesis and diagnosis]
    Cassian Sitaru
    Universitäts Hautklinik, Wurzburg
    J Dtsch Dermatol Ges 2:123-8; quiz 139-40. 2004
  44. ncbi request reprint IgG autoantibodies to type VII collagen and an exclusive IgG3 reactivity to the laminin alpha3 chain in a patient with an autoimmune subepidermal blistering disease
    Adrian Baican
    Department of Dermatology, University of Medicine and Pharmacy Cluj Napoca, Cluj Napoca, Romania
    J Am Acad Dermatol 53:517-22. 2005
    ..We report a most unusual case of an autoimmune subepidermal blistering with an exclusive IgG3 reactivity to laminin 5...
  45. ncbi request reprint Autoantibodies from patients with BSLE inducing recruitment of leukocytes to the dermoepidermal junction and subepidermal splits in cryosections of human skin
    Josep E Herrero-Gonzalez
    Arch Dermatol 142:1513-6. 2006
  46. ncbi request reprint IgA pemphigus--occurrence of anti-desmocollin 1 and anti-desmoglein 1 antibody reactivity in an individual patient
    Tamara Kopp
    Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna General Hospital, Austria
    J Dtsch Dermatol Ges 4:1045-50. 2006
    ....
  47. ncbi request reprint Spatiotemporal distribution of Fras1/Frem proteins during mouse embryonic development
    Rena Chiotaki
    Department of Biology, University of Crete, 71409 Heraklion, Crete, Greece
    Gene Expr Patterns 7:381-8. 2007
    ..We show that basement membrane levels of collagen VII rise at late embryonic life, concomitant with descending Fras1/Frem immunolabeling...
  48. ncbi request reprint Subclass distribution of type VII collagen-specific autoantibodies in patients with inflammatory bowel disease
    Gertie Janneke Oostingh
    J Dermatol Sci 37:182-4. 2005
  49. ncbi request reprint Cicatricial pemphigoid differs from bullous pemphigoid and pemphigoid gestationis regarding the fine specificity of autoantibodies to the BP180 NC16A domain
    Arno Kromminga
    Institute for Immunology, Clinical Pathology and Molecular Medicine IPM, Hamburg, Germany
    J Dermatol Sci 28:68-75. 2002
    ..Our data demonstrate a heterogeneous binding pattern of autoantibodies to BP180 NC16A in patients with CP...
  50. doi request reprint Bullous pemphigoid autoantibodies preferentially recognize phosphoepitopes in collagen XVII
    Elena P Zimina
    J Invest Dermatol 128:2736-9. 2008
  51. ncbi request reprint Comparative analysis of methods for detection of anti-laminin 5 autoantibodies in patients with anti-epiligrin cicatricial pemphigoid
    Zelmira Lazarova
    Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 4801, USA
    J Am Acad Dermatol 51:886-92. 2004
    ..Anti-epiligrin cicatricial pemphigoid (AECP) is a subepidermal blistering disease characterized by circulating anti-basement membrane autoantibodies to laminin 5...
  52. ncbi request reprint [Blistering autoimmune dermatoses (II): therapy]
    Matthias Goebeler
    Klinik und Poliklinik für Haut und Geschlechtskrankheiten, Bayerische Julius Maximilians Universität Würzburg
    J Dtsch Dermatol Ges 2:774-91; quiz 792-3. 2004
  53. ncbi request reprint [Anti-laminin 5 mucous membrane pemphigoid]
    Sandrine Benoit
    Universitäts Hautklinik Würzburg, Josef Schneider Str 2, D 97080 Wurzburg
    J Dtsch Dermatol Ges 4:41-4. 2006
    ..Based on these results, the diagnosis of an anti-laminin 5 mucous membrane pemphigoid was established. Oral treatment with dapsone, combined with intravenous dexamethasone pulse therapy, led to rapid improvement...
  54. ncbi request reprint [Mucous membrane pemphigoid: IgG and IgA antibodies against the BP180 antigen]
    Agustín España
    Departamento de Dermatologia, Clinica Universitaria de Navarra, Pamplona, Spain
    Actas Dermosifiliogr 96:365-70. 2005
    ..Mucous membrane pemphigoid is a group of autoimmune bullous diseases, mediated by autoantibodies directed against different proteins in the dermoepidermal junction, including the BP180 antigen...