Michelle A Digman

Summary

Publications

  1. pmc Mapping the number of molecules and brightness in the laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2320-32. 2008
  2. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
  3. pmc Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 102:691-7. 2012
  4. pmc The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 100:1829-36. 2011
  5. pmc Stoichiometry of molecular complexes at adhesions in living cells
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering and Development Biology Center Optical Biology Core Facility, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 106:2170-5. 2009
  6. doi request reprint Analysis of diffusion and binding in cells using the RICS approach
    Michelle A Digman
    Optical Biology Core, University of California, Irvine, California 92697, USA
    Microsc Res Tech 72:323-32. 2009
  7. pmc In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 107:16560-5. 2010
  8. pmc Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method
    Michelle A Digman
    Laboratory for Fluorescence Dynamics and Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 96:707-16. 2009
  9. pmc Lessons in fluctuation correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92697, USA
    Annu Rev Phys Chem 62:645-68. 2011
  10. pmc Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2819-31. 2008

Collaborators

  • Enrico Gratton
  • Francesco Cardarelli
  • C M Brown
  • Klaus M Hahn
  • Rahul Singh
  • Qing Nie
  • Hector Giral
  • Olivier Cinquin
  • W Cho
  • N Sidenius
  • Makoto Inoue
  • Elizabeth Hinde
  • Laura M Andrews
  • Chiara Stringari
  • Mark R Jones
  • Ekaterina Kniazeva
  • Shaohua Zhou
  • Belinda K Wright
  • Arthur D Lander
  • Ryan L Stafford
  • Luca Lanzano
  • Robert V Stahelin
  • Molly J Rossow
  • Giulia Ossato
  • Wan Jong Kuo
  • Bharath Ananthanarayanan
  • Rooshin B Dalal
  • Valeria R Caiolfa
  • Nikhil A Gokhale
  • Maria Luisa Barcellona
  • Andrew J Putnam
  • Jeffrey L Suhalim
  • Christopher Welch
  • Wing Cheong Lo
  • Elliot L Botvinick
  • John W Weidling
  • Amanda Cinquin
  • Peter J Donovan
  • Judith Kimble
  • Mario A Pennella
  • Aaron Kershner
  • Peter Fwu
  • Jason Ear
  • James U Bowie
  • Mary Jane Knight
  • Moshe Levi
  • J Lawrence Marsh
  • Jennifer M Sasaki
  • Charity Aiken
  • Tamas Lukacsovich
  • Alan F Horwitz
  • Valeria Vetri
  • Chris D Madsen
  • Francesco Blasi
  • Gabriele Malengo
  • Jason Sutin
  • Moreno Zamai
  • Annapaola Andolfo
  • Alexandra Abraham
  • John D Rafter
  • Martina Medkova
  • Theodore Hazlett
  • Heather R Melowic
  • Seth Gammon

Detail Information

Publications35

  1. pmc Mapping the number of molecules and brightness in the laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2320-32. 2008
    ....
  2. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
    ..This new type of data analysis has a broad application in biology and it provides a powerful tool for measuring fast as well as slower dynamic processes in cellular systems using any standard laser confocal microscope...
  3. pmc Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 102:691-7. 2012
    ..For the first time, to our knowledge, we were able to probe chromatin compaction on the micrometer scale, where the regulation of molecular diffusion may become relevant for many cellular processes...
  4. pmc The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 100:1829-36. 2011
    ..These two distinct routes of molecular flow were concomitantly measured in the Caenorhabditis elegans germ line, which indicates a conservation of mechanism on a scale more widespread than cell type or organism...
  5. pmc Stoichiometry of molecular complexes at adhesions in living cells
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering and Development Biology Center Optical Biology Core Facility, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 106:2170-5. 2009
    ..These aggregates disassemble rapidly in the cytoplasm because large complexes are found only in very close proximity to the adhesions or at their borders...
  6. doi request reprint Analysis of diffusion and binding in cells using the RICS approach
    Michelle A Digman
    Optical Biology Core, University of California, Irvine, California 92697, USA
    Microsc Res Tech 72:323-32. 2009
    ..We apply the method to determine the diffusion in the cytoplasm and binding of Focal Adhesion Kinase-EGFP to adhesions in MEF cells...
  7. pmc In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 107:16560-5. 2010
    ..This is a unique in vivo demonstration of the intricate chromatin network showing channel directed diffusion of an inert molecule with high spatial and temporal resolution...
  8. pmc Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method
    Michelle A Digman
    Laboratory for Fluorescence Dynamics and Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 96:707-16. 2009
    ..This fluctuation imaging method can be applied to commercial laser scanning microscopes thereby making it accessible to a large community of scientists...
  9. pmc Lessons in fluctuation correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92697, USA
    Annu Rev Phys Chem 62:645-68. 2011
    ..Image fluctuation analysis has become a rich and powerful technique that can be used to extract information about the spatial distribution of molecular concentration and transport in cells and tissues...
  10. pmc Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2819-31. 2008
    ....
  11. pmc Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92617, USA
    Proc Natl Acad Sci U S A 110:135-40. 2013
    ..This multiplexed approach to biosensor FRET detection serves as a unique tool for dissection of the mechanism(s) by which key signaling proteins are spatially and temporally coordinated...
  12. pmc Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Microsc Res Tech 75:271-81. 2012
    ....
  13. pmc Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 88:L33-6. 2005
    ..We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale...
  14. pmc Raster image correlation spectroscopy in live cells
    Molly J Rossow
    Department of Biomedical Engineering, University of California Irvine, Irvine, California, USA
    Nat Protoc 5:1761-74. 2010
    ..This procedure includes focal volume calibration using a solution of fluorophores with a known diffusion coefficient and measurement of the diffusion coefficients of cytosolic enhanced green fluorescent protein (EGFP) and EGFP-paxillin...
  15. pmc Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D
    Ekaterina Kniazeva
    Biomedical Engineering Department, Natural Sciences II, Room 3201, University of California, Irvine, Irvine, CA 92697 2715, USA
    Integr Biol (Camb) 4:431-9. 2012
    ....
  16. pmc Free extracellular diffusion creates the Dpp morphogen gradient of the Drosophila wing disc
    Shaohua Zhou
    Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA
    Curr Biol 22:668-75. 2012
    ..The strongest support for the view that morphogens do not simply spread by free diffusion has come from a variety of studies of the Decapentaplegic (Dpp) gradient of the Drosophila larval wing disc...
  17. pmc Scanning image correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of California, Irvine, CA, USA
    Bioessays 34:377-85. 2012
    ..These methods can be implemented with a standard laser scanning microscope and produce a cellular level spatio-temporal map of molecular interactions...
  18. pmc Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK
    Ryan L Stafford
    Department of Chemistry and Biochemistry, UCLA DOE Institute of Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095 1570, USA
    Structure 19:1826-36. 2011
    ..The Caskin1 polymers can be decorated with CASK proteins, illustrating how these proteins may work together to organize the cytomatrix in active zones...
  19. pmc Nanometer-scale imaging by the modulation tracking method
    Luca Lanzano
    Laboratory for Fluorescence Dynamics, University of California Biomedical Engineering, 3210 Natural Sciences 2, Irvine, California 92672, USA
    J Biophotonics 4:415-24. 2011
    ..Our approach to super-resolution and to 3D nanoimaging is different than other proposed methods that break the diffraction limit using non-linear effects or are based on single molecule localization...
  20. pmc Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
    Wiley Interdiscip Rev Syst Biol Med 1:273-82. 2009
    ..Although one example of the application of the fluctuation method is given, this article also contains simulations that are better suited to illustrate and support the basic assumptions of the method...
  21. pmc Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue
    Chiara Stringari
    Laboratory of Fluorescence Dynamics, Biomedical Engineering Department, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 108:13582-7. 2011
    ..Our method is a promising noninvasive optical tool for monitoring metabolic pathways during differentiation or disease progression, and for cell sorting in unlabeled tissues...
  22. pmc Imaging barriers to diffusion by pair correlation functions
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 97:665-73. 2009
    ..It does not require isolated molecules, and thus many molecules can be labeled at the same time and within the point spread function...
  23. doi request reprint Raster image correlation spectroscopy and number and brightness analysis
    Michelle A Digman
    Department of Biomedical Engineering, University of California, Irvine, California, USA
    Methods Enzymol 518:121-44. 2013
    ..We also discuss common control experiments needed to rule out instrumental artifacts and how to calibrate the microscope in terms of relative molecular brightness...
  24. pmc A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis
    Giulia Ossato
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 98:3078-85. 2010
    ..The behavior of Httex1p in COS-7 and ST14A cells is compared...
  25. pmc Phasor-FLIM analysis of NADH distribution and localization in the nucleus of live progenitor myoblast cells
    Belinda K Wright
    School of Science and Health, University of Western Sydney, Hawkesbury, New South Wales, Australia
    Microsc Res Tech 75:1717-22. 2012
    ..Overall our results suggest an order of events in which a cell metabolic status alters significantly during the early stages of serum induced differentiation...
  26. pmc Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells
    Laura M Andrews
    University of Western Sydney, School of Science and Health, Richmond, New South Wales, Australia Laboratory for Fluorescence Dynamics LFD, University of California, Biomedical Engineering Department, Irvine, California 92697 2715, USA
    Biomed Opt Express 4:171-7. 2013
    ..In addition, transcripts in the cytoplasm undertook a filamentous morphology composed of multiple puncti structures which individually localized along and close to mitochondria but were distinct from mitochondria...
  27. pmc Heparan sulfate acts as a bone morphogenetic protein coreceptor by facilitating ligand-induced receptor hetero-oligomerization
    Wan Jong Kuo
    Department of Developmental and Cell Biology, Center for Complex Biological Systems, University of California Irvine, Irvine, CA 92697, USA
    Mol Biol Cell 21:4028-41. 2010
    ..This suggests a view of HS as a catalyst of the formation of signaling complexes, rather than as a stabilizer of growth factor binding...
  28. pmc The phasor approach to fluorescence lifetime imaging analysis
    Michelle A Digman
    Biophys J 94:L14-6. 2008
    ....
  29. ncbi request reprint Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode
    Rooshin B Dalal
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    Microsc Res Tech 71:69-81. 2008
    ..Furthermore, we distinguish between mobile and immobile components, and introduce a method to correct for slow variations in intensity...
  30. ncbi request reprint Mechanism of diacylglycerol-induced membrane targeting and activation of protein kinase Cdelta
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 279:29501-12. 2004
    ..Collectively, these results provide new insight into the differential roles of C1 domains in the DAG-induced membrane activation of PKCdelta and the origin of its specific subcellular localization in response to DAG...
  31. ncbi request reprint Partitioning of NaPi cotransporter in cholesterol-, sphingomyelin-, and glycosphingolipid-enriched membrane domains modulates NaPi protein diffusion, clustering, and activity
    Makoto Inoue
    Department of Medicine, University of Colorado Health Sciences Center and Denver Veterans Affairs Medical Center, Denver, Colorado 80262, USA
    J Biol Chem 279:49160-71. 2004
    ....
  32. ncbi request reprint Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes
    Maria Luisa Barcellona
    Department of Biological Chemistry and Molecular Biology, University of Catania, Catania, Italy
    Microsc Res Tech 65:205-17. 2004
    ....
  33. ncbi request reprint Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer
    Nikhil A Gokhale
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607 7061, USA
    J Biol Chem 280:42831-40. 2005
    ..Collectively, these studies elucidate the structural determinant of the PtdIns(4,5)P2 selectivity of A2t and suggest that A2t may be involved in the regulation of PtdIns(4,5)P2 clustering in the cell...
  34. pmc Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies
    Valeria R Caiolfa
    Department of Molecular Biology and Functional Genomics, Unit of Molecular Neuroscience, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Cell Biol 179:1067-82. 2007
    ..These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents...
  35. ncbi request reprint Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains
    Bharath Ananthanarayanan
    Department of Chemistry, University of Illinois at Chicago, 845 W Taylor Street, Chicago, IL 60607, USA
    J Biol Chem 278:46886-94. 2003
    ..These data suggest that differential activation mechanisms of PKC isoforms are determined by the DAG affinity and conformational flexibility of their C1 domains...