The promise, pitfalls and progress of RNA-interference-based antiviral therapy for respiratory virusesJohn P DeVincenzo
Department of Pediatrics, University of Tennessee Health Sciences Center, University of Tennessee, Memphis, TN, USA
Antivir Ther 17:213-25. 2012
..This review discusses the science behind RNAi and the potential practical issues in applying this technology to various respiratory viral diseases...
Viral load drives disease in humans experimentally infected with respiratory syncytial virusJohn P DeVincenzo
Department of Pediatrics, University of Tennessee College of Medicine, Memphis, USA
Am J Respir Crit Care Med 182:1305-14. 2010
..Two major challenges have stalled antiviral development: ethical difficulties in performing pediatric proof-of-concept studies and the prevailing concept that the disease is immune-mediated rather than being driven by viral load...
- Respiratory syncytial virus load, viral dynamics, and disease severity in previously healthy naturally infected children
Chadi M El Saleeby
Division of Pediatric Infectious Diseases, Department of Pediatrics, Harvard Medical School, Massachusetts General Hospital for Children, Boston, USA
J Infect Dis 204:996-1002. 2011
..The relationships between RSV load, viral clearance dynamics, and disease severity have not been carefully evaluated...
- Surfactant protein A2 polymorphisms and disease severity in a respiratory syncytial virus-infected population
Chadi M El Saleeby
Department of Pediatrics, Harvard Medical School, Massachusetts General Hospital for Children, Boston, MA 02114, USA
J Pediatr 156:409-14. 2010
..To examine whether genetic variations within the surfactant protein A2 (SP-A2) gene are associated with respiratory syncytial virus (RSV) disease severity in infected children...
Natural infection of infants with respiratory syncytial virus subgroups A and B: a study of frequency, disease severity, and viral loadJohn P DeVincenzo
Department of Pediatrics, University of Tennessee, LeBonheur Children s Medical Center, Memphis, TN 38103, USA
Pediatr Res 56:914-7. 2004
..Disease severity risk factors were similar between subgroups. RSV loads were similar between A and B subgroups (4.77 versus 4.68 log PFU/mL). Measures of disease severity were also similar between subgroups...
- Respiratory syncytial virus load predicts disease severity in previously healthy infants
John P DeVincenzo
Department of Pediatrics, University of Tennessee School of Medicine, Memphis, Tennessee, USA
J Infect Dis 191:1861-8. 2005
..Elucidating the relationship between viral load and respiratory syncytial virus (RSV) disease severity is critical to understanding pathogenesis and predicting the utility of antivirals...
- Harnessing RNA interference to develop neonatal therapies: from Nobel Prize winning discovery to proof of concept clinical trials
John P DeVincenzo
University of Tennessee School of Medicine, Department of Pediatrics, LeBonheur Children s Medical Center, Room 433, 50N Dunlap Street, Memphis, TN 38103, USA
Early Hum Dev 85:S31-5. 2009
..RNAi may offer new strategies for the treatment of a variety of orphan diseases including neonatal diseases, RSV infections, and other respiratory viruses...
- RNA interference strategies as therapy for respiratory viral infections
John P DeVincenzo
Department of Pediatrics and Molecular Sciences, University of Tennessee, The Children s Foundation Research Center, and LeBonheur Children s Medical Center, Memphis, TN 38103, USA
Pediatr Infect Dis J 27:S118-22. 2008
..RNAi may offer new strategies for the treatment of respiratory syncytial virus and other respiratory viruses...
- Surveillance of clinical isolates of respiratory syncytial virus for palivizumab (Synagis)-resistant mutants
John P DeVincenzo
University of Tennessee, LeBonheur Children s Medical Center, Memphis 38103, USA
J Infect Dis 190:975-8. 2004
..Palivizumab bound to all 371 RSV isolates able to be evaluated, including 25 from active-palivizumab recipients. The palivizumab epitope appears to be highly conserved, even in infants receiving prophylaxis with palivizumab...
- Respiratory syncytial virus (RSV) loads in premature infants with and without prophylactic RSV fusion protein monoclonal antibody
John P DeVincenzo
Department of Pediatrics, University of Tennessee, LeBonheur Children s Medical Center, and the Children s Foundation Research Center, Memphis, Tennessee 38103, USA
J Pediatr 143:123-6. 2003
..Mean (SEM) RSV loads in the MAb and non-MAb groups were 3.36 (0.59) versus 4.89 (0.27) logPFU/ml (P=.01). Prophylactic palivizumab reduces nasal RSV in premature hospitalized infants...
- Factors predicting childhood respiratory syncytial virus severity: what they indicate about pathogenesis
John P DeVincenzo
Department of Pediatrics, University of Tennessee School of Medicine, Memphis, TN, USA
Pediatr Infect Dis J 24:S177-83, discussion S182. 2005
..Reviewing and interpreting the clinical risk factors to gain an insight into RSV pathogenesis is important, especially considering the relative lack of parallel between many animal models of RSV infection and observed human disease...
- Risk factors for severe respiratory syncytial virus disease in children with cancer: the importance of lymphopenia and young age
Chadi M El Saleeby
Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale St, Memphis, TN 38105, USA
Pediatrics 121:235-43. 2008
..We sought to determine the epidemiologic features of respiratory syncytial virus infection in immunocompromised pediatric patients and to identify the risk factors for severe disease...
- Comparison of a real-time reverse transcriptase PCR assay and a culture technique for quantitative assessment of viral load in children naturally infected with respiratory syncytial virus
Stephanie M Perkins
Unversity of Tennessee School of Medicine, Memphis, TN, USA
J Clin Microbiol 43:2356-62. 2005
..The utility of the RTrtPCR quantification technique for clinical studies would be solidified after its correlation with RSV disease severity is established...
- Human genetic factors and respiratory syncytial virus disease severity
Isao Miyairi
Department of Pediatrics, University of Tennessee, Memphis, Tennessee 38103, USA
Clin Microbiol Rev 21:686-703. 2008
..Such problems highlight the limitation of the candidate gene approach and the need for an unbiased large-scale genome-wide association study to evaluate this important disease...
- 2007 International Congress on Respiratory Viruses. Introduction
John P DeVincenzo
Department of Pediatrics and Molecular Sciences, University of Tennessee, The Children's Foundation Research Center, and LeBonheur Children's Medical Center, Memphis, TN 38103, USA
Pediatr Infect Dis J 27:S53. 2008
- A randomized, double-blind, placebo-controlled trial of dexamethasone in severe respiratory syncytial virus (RSV) infection: effects on RSV quantity and clinical outcome
Steven C Buckingham
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN, USA
J Infect Dis 185:1222-8. 2002
....
- Opportunities for early therapy of respiratory syncytial virus (RSV) infection: what happens before hospitalization
John P DeVincenzo
Department of Pediatrics, University of Tennessee and the Children s Foundation Research Center, 4th floor CFRC, LBCMC, 50 North Dunlap St, Memphis, TN 38103, USA
Antiviral Res 62:47-51. 2004
..6 +/- 1.8 days. RSV-hospitalized infants routinely have contact with health care professionals at significantly early time points in their illness. These contacts are potential opportunities for initiation of early anti-RSV therapy...
- Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner
Vasanthi Avadhanula
Department of Infectious Diseases, St. Jude Children's Research Hospital, Mailstop 320, 332 N. Lauderdale St, Memphis, TN 38105, USA
J Virol 80:1629-36. 2006
..These studies highlight the distinct responses of different cell types to viral infection and the need to consider this variation when interpreting studies of the interactions between respiratory cells and viral pathogens...
- Antibodies reactive to Rickettsia rickettsii among children living in the southeast and south central regions of the United States
Gary S Marshall
Department of Pediatrics, University of Louisville School of Medicine, Louisville, KY40202, USA
Arch Pediatr Adolesc Med 157:443-8. 2003
..This study estimated the prevalence of antibodies reactive to R rickettsii among children living in the southeastern and south central United States...
- Group B streptococcal ventriculitis: a report of three cases and literature review
Isao Miyairi
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA
Pediatr Neurol 34:395-9. 2006
..One child died, and six of nine survivors were left with significant neurologic deficits. Physicians should be aware of this indolent but serious manifestation of group B streptococcal infection...
