Mordechai Deutsch

Summary

Publications

  1. ncbi request reprint Donut-shaped chambers for analysis of biochemical processes at the cellular and subcellular levels
    N Zurgil
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, 52900, Ramat Gan, Israel
    Lab Chip 14:2226-39. 2014
  2. pmc The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: methodology
    Mordechai Deutsch
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    BMC Cell Biol 11:54. 2010
  3. doi request reprint Comparative analysis of cell parameter groups for breast cancer detection
    David Blokh
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Comput Methods Programs Biomed 94:239-49. 2009
  4. pmc The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: functional activity of cryopreserved cells
    Elena Afrimzon
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan, Israel
    BMC Cell Biol 11:83. 2010
  5. ncbi request reprint A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids
    Yael Markovitz-Bishitz
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    Biomaterials 31:8436-44. 2010
  6. doi request reprint The use of sequential staining for detection of heterogeneous intracellular response of individual Jurkat cells to lysophosphatidylcholine
    Elena Afrimzon
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    J Immunol Methods 387:96-106. 2013
  7. doi request reprint Functional analysis of individual cells and microenvironment of breast cancer-draining lymph nodes
    Naomi Zurgil
    The Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan, 52900, Israel
    Cancer Sci 99:936-45. 2008
  8. ncbi request reprint The information-theory analysis of Michaelis-Menten constants for detection of breast cancer
    David Blokh
    The Biophysical Interdisciplinary Jerome Schottenstein Center for Research and Technology of Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Cancer Detect Prev 31:489-98. 2007
  9. ncbi request reprint Concomitant real-time monitoring of intracellular reactive oxygen species and mitochondrial membrane potential in individual living promonocytic cells
    Naomi Zurgil
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    J Immunol Methods 316:27-41. 2006
  10. doi request reprint Polymer live-cell array for real-time kinetic imaging of immune cells
    Naomi Zurgil
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel
    Biomaterials 31:5022-9. 2010

Collaborators

  • Elena Afrimzon
  • Itzhak Pappo
  • Heiko Zimmermann
  • Alon Korngreen
  • Lior Turgeman
  • Naomi Zurgil
  • Yana Shafran
  • Dror Fixler
  • David Blokh
  • Meir Cohen-Kashi
  • Yaniv Namer
  • Assaf Deutsch
  • Maria Sobolev
  • Yishay Tauber
  • Shoshy Herman
  • Judith Sandbank
  • Eden Korech
  • Ilia Stambler
  • N Zurgil
  • Tzachi Sabati
  • Yael Markovitz-Bishitz
  • Asher Shainberg
  • Merav Sunray
  • Zeev Schiffer
  • Reuven Tirosh
  • Boris Gilburd
  • Yehuda Shoenfeld
  • Menachem Kaufman
  • Michael Ehrenfeld
  • Pnina Langevitz
  • Yitzhak Yishai
  • E Shinar
  • J He
  • M Sobolev
  • Y Shafran
  • O Ravid-Hermesh
  • R Goldman-Levi
  • S Howitz
  • Bat Sheva Galmidi
  • Steffen Howitz
  • Orit Ravid-Hermesh
  • Max Platkov
  • Ora Shovman
  • Haim Brikman
  • Ruben Orda
  • Yitshak Yishay
  • Ihar Hurevich
  • Pnina Lebovich
  • Michal Haran
  • David Bloch
  • Rami Avtalion
  • Inna Solodeev
  • Sergey Moshkov

Detail Information

Publications32

  1. ncbi request reprint Donut-shaped chambers for analysis of biochemical processes at the cellular and subcellular levels
    N Zurgil
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, 52900, Ramat Gan, Israel
    Lab Chip 14:2226-39. 2014
    ..These unique DSC arrays are easy to manufacture and offer an inexpensive and simple operating system for biochemical reaction measurement of numerous single cells used in various practical applications. ..
  2. pmc The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: methodology
    Mordechai Deutsch
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    BMC Cell Biol 11:54. 2010
    ..The methods currently used to retrieve a specific cell or a group of individual cells with specific biological properties after cryopreservation are quite complicated and inefficient...
  3. doi request reprint Comparative analysis of cell parameter groups for breast cancer detection
    David Blokh
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Comput Methods Programs Biomed 94:239-49. 2009
    ..The FP parameters were grouped according to their correlation with breast cancer. It was established that the greatest difference between cells of BC patients and healthy subjects is found in the PHA test (parameter P1)...
  4. pmc The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: functional activity of cryopreserved cells
    Elena Afrimzon
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan, Israel
    BMC Cell Biol 11:83. 2010
    ....
  5. ncbi request reprint A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids
    Yael Markovitz-Bishitz
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    Biomaterials 31:8436-44. 2010
    ..The advantages of this spheroid-based system make it an efficient drug-screening tool that may be valuable to related fields of research and clinical applications...
  6. doi request reprint The use of sequential staining for detection of heterogeneous intracellular response of individual Jurkat cells to lysophosphatidylcholine
    Elena Afrimzon
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    J Immunol Methods 387:96-106. 2013
    ....
  7. doi request reprint Functional analysis of individual cells and microenvironment of breast cancer-draining lymph nodes
    Naomi Zurgil
    The Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan, 52900, Israel
    Cancer Sci 99:936-45. 2008
    ..Thus, the live and postfixation features of LN cells and their microenvironment, correlated with the functional status of the LN, may serve to improve their predictive value in breast cancer examination...
  8. ncbi request reprint The information-theory analysis of Michaelis-Menten constants for detection of breast cancer
    David Blokh
    The Biophysical Interdisciplinary Jerome Schottenstein Center for Research and Technology of Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Cancer Detect Prev 31:489-98. 2007
    ..The Michaelis-Menten constants (K(m) and V(max)) operated by the Information Theory were employed for detection of breast cancer...
  9. ncbi request reprint Concomitant real-time monitoring of intracellular reactive oxygen species and mitochondrial membrane potential in individual living promonocytic cells
    Naomi Zurgil
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    J Immunol Methods 316:27-41. 2006
    ..The data demonstrated that temporal measurement of ROS generation, in identifiable individual cells, reveals kinetic behavior that otherwise would be undetected...
  10. doi request reprint Polymer live-cell array for real-time kinetic imaging of immune cells
    Naomi Zurgil
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel
    Biomaterials 31:5022-9. 2010
    ..These properties facilitate real-time, non-intrusive, repeated measurements of immune cells under multiple experimental treatments...
  11. ncbi request reprint The immunosuppressive effect of methotrexate in active rheumatoid arthritis patients vs. its stimulatory effect in nonactive patients, as indicated by cytometric measurements of CD4+ T cell subpopulations
    Shoshy Herman
    Physics Department, The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Bar Ilan University, Ramat Gan, Israel
    Immunol Invest 33:351-62. 2004
    ..The found discriminative effect of MTX may suggest a higher effectiveness of low-dose MTX therapy in active RA patients...
  12. doi request reprint Intracellular esterase activity in living cells may distinguish between metastatic and tumor-free lymph nodes
    Elena Afrimzon
    Department of Physics, The Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Bar Ilan University, Ramat Gan 52900, Israel
    Clin Exp Metastasis 25:213-24. 2008
    ..The purpose of our study was to investigate functional metabolic characteristics of living cells derived from metastatic and tumor-free lymph nodes of breast cancer (BC) patients...
  13. ncbi request reprint Monitoring the apoptotic process induced by oxidized low-density lipoprotein in Jurkat T-lymphoblast and U937 monocytic human cell lines
    Naomi Zurgil
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan, Israel
    Cell Biochem Biophys 40:97-113. 2004
    ..Such data suggest that reactive oxygen species generation are involved in Ox-LDL-induced apoptosis and that monocytes are more susceptible to cell death triggered by oxidative stress...
  14. ncbi request reprint A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells
    Mordechai Deutsch
    Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel
    Lab Chip 6:995-1000. 2006
    ..Several exemplary experiments, using a microscope-slide-based version of the CR, are presented, which could not be performed by other state of the art methods...
  15. ncbi request reprint Correlative analyses of nitric oxide generation rates and nitric oxide synthase levels in individual cells using a modular cell-retaining device
    Yana Shafran
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar Ilan University, Ramat Gan, 52900 Israel
    Anal Chem 84:7315-22. 2012
    ....
  16. ncbi request reprint Lymphocyte resistance to lysophosphatidylcholine mediated apoptosis in atherosclerosis
    Naomi Zurgil
    The Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan, Israel
    Atherosclerosis 190:73-83. 2007
    ..The aim of the present study was to evaluate the apoptotic effect of lysophosphatidylcholine (LPC) on peripheral blood lymphocytes (PBL) derived from unstable angina (UA) patients, as compared to healthy donors...
  17. ncbi request reprint Monitoring of intracellular enzyme kinetic characteristics of peripheral mononuclear cells in breast cancer patients
    Elena Afrimzon
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan, Israel
    Cancer Epidemiol Biomarkers Prev 13:235-41. 2004
    ..Thus, the present methodology may serve as an additional criterion for prognosis and monitoring, both in BC patients, and in individuals associated with high cancer risk...
  18. ncbi request reprint A cluster pattern algorithm for the analysis of multiparametric cell assays
    Menachem Kaufman
    The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan, Israel
    J Comput Biol 12:1014-28. 2005
    ..It relies on the multidimensional pattern of the sample parameters. The algorithm compensates for environmental drifts in this apparatus and assay; it also may be applied to more than four dimensions...
  19. ncbi request reprint Influence of fluorescence anisotropy on fluorescence intensity and lifetime measurement: theory, simulations and experiments
    Dror Fixler
    School of Engineering, Bar Ilan University, Ramat Gan, Israel
    IEEE Trans Biomed Eng 53:1141-52. 2006
    ....
  20. ncbi request reprint Tracing apoptosis and stimulation in individual cells by fluorescence intensity and anisotropy decay
    Dror Fixler
    Bar Ilan University, School of Engineering, Ramat Gan 52900, Israel
    J Biomed Opt 10:034007. 2005
    ....
  21. ncbi request reprint Energy transfer efficiency on lymphocytes of leukemia patients measured by fluorescence polarization
    Meir Cohen-Kashi
    Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan, Israel
    Leuk Lymphoma 46:113-20. 2005
    ..Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 +/- 3, whereas in the CLL patients it was significantly lower (8 +/- 5) (P < 0.01)...
  22. ncbi request reprint Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population
    Assaf Deutsch
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Biomed Microdevices 8:361-74. 2006
    ..The PW's unique micro-optical features enable rapid, simultaneous signal analysis of each individual cell, bypassing lengthy image analysis...
  23. ncbi request reprint Fluorescence resonance energy transfer imaging via fluorescence polarization measurement
    Meir Cohen-Kashi
    Bar Ilan University, Department of Physics, Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Ramat Gan, Israel
    J Biomed Opt 11:34015. 2006
    ..It shows not only that FRET imaging via FP is possible, but also that it is inexpensive, simple to perform, conveniently adaptable to the commonly used fluorescent microscopy, and readily interpretable...
  24. ncbi request reprint A novel approach for on line monitoring of apoptotic cell shrinkage in individual live lymphocytes
    Naomi Zurgil
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Department of Physics, Bar Ilan University, 52900 Ramat Gan, Israel
    J Immunol Methods 281:37-49. 2003
    ..The ability to remeasure the same cell before and after apoptosis induction enabled the detection of specific individual lymphocytes, which were more susceptible or resistant to pro-apoptotic stimulus...
  25. doi request reprint Whole-object fluorescence lifetime setup for efficient non-imaging quantitative intracellular fluorophore measurements
    Yaniv Namer
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan, Israel
    J Fluoresc 22:875-82. 2012
    ..The proposed wo-FLT approach may be of particular importance when direct imaging is impossible...
  26. ncbi request reprint Real-time monitoring of changes in plasma membrane potential via imaging of fluorescence resonance energy transfer at individual cell resolution in suspension
    Tzachi Sabati
    Bar Ilan University, The Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Ramat Gan, Israel
    J Biomed Opt 18:126010. 2013
    ..The spatial resolution of E% plots seem to be higher, and their contrast greater, when calculated from the polarization, rather than from the intensity of the fluorescence. ..
  27. ncbi request reprint Particle-sizing methods: a stationary-phase-based comparison
    Zeev Schiffer
    Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, University of Bar Ilan, Ramat Gan, Israel
    Appl Opt 46:2209-18. 2007
    ..Simulation and experimental results for both differential light scattering and microscope imaging of polystyrene beads are in good agreement with theory...
  28. ncbi request reprint Breast cancer detection by Michaelis-Menten constants via linear programming
    David Blokh
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Comput Methods Programs Biomed 85:210-3. 2007
    ..Out of 50 subjects tested, 44 were diagnosed correctly, in 5 cases the diagnosis was not certain, and 1 subject was diagnosed incorrectly...
  29. ncbi request reprint Ratiometric fluorescence polarization as a cytometric functional parameter: theory and practice
    Yitzhak Yishai
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan 52900, Israel
    Phys Med Biol 48:2255-68. 2003
    ..In the third bioassay, cellular thiol level of FDA- and CMFDA-labelled Jurkat T cells was monitored via RFP...
  30. ncbi request reprint The induction of apoptosis by methotrexate in activated lymphocytes as indicated by fluorescence hyperpolarization: a possible model for predicting methotrexate therapy for rheumatoid arthritis patients
    Shoshy Herman
    The Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel
    Cell Struct Funct 28:113-22. 2003
    ..Moreover, the FP measurements can be used for the diagnosis, and making timing and dosage decisions...
  31. pmc Fluorescence resonance energy transfers measurements on cell surfaces via fluorescence polarization
    Meir Cohen-Kashi
    The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome Physics Department, Bar Ilan University, Ramat Gan 52900, Israel
    Biophys J 83:1395-402. 2002
    ..The experiments and results clearly indicate that determination of E via FP measurements is possible, efficient, and more convenient than other methods...
  32. ncbi request reprint Determination of individual cell Michaelis-Menten constants
    Merav Sunray
    The Jerome Schottenstein Center for Early Detection of Cancer, Department of Physics, Bar Ilan University, Ramat Gan, Israel
    Cytometry 47:8-16. 2002
    ..The mathematical formulation was approximated linearly in order to analyze intracellular substrate conversion characteristics via Michaelis-Menten theory...