R E Weber
Affiliation: University of Aarhus
- Enthalpic consequences of reduced chloride binding in Andean frog (Telmatobius peruvianus) hemoglobinRoy E Weber
Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark
J Comp Physiol B 184:613-21. 2014..The high temperature sensitivity associated with decreased chloride binding and low phosphate sensitivity of Telmatobius Hb likely promotes cutaneous O2 uptake in cold, high-altitude ponds and streams. ..
- Lack of conventional oxygen-linked proton and anion binding sites does not impair allosteric regulation of oxygen binding in dwarf caiman hemoglobinRoy E Weber
Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark
Am J Physiol Regul Integr Comp Physiol 305:R300-12. 2013..palpebrosus Hb provides a unique opportunity for studies on structure-function coupling and the evolution of compensatory mechanisms for maintaining tissue O2 delivery in Hbs that lack conventional effector-binding residues. ..
- Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial moleKevin L Campbell
Department of Biological Sciences, University of Manitoba, Winnipeg, Canada
BMC Evol Biol 10:214. 2010..g., 2,3-diphosphoglycerate; DPG), or by suppressing the sensitivity of Hb to these physiological cofactors...
- Globin-like proteins in Caenorhabditis elegans: in vivo localization, ligand binding and structural propertiesEva Geuens
Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B 2610 Antwerp, Belgium
BMC Biochem 11:17. 2010..No information is available on the physicochemical properties and the in vivo expression...
- Thermodynamics of oxygenation-linked proton and lactate binding govern the temperature sensitivity of O2 binding in crustacean (Carcinus maenas) hemocyaninRoy E Weber
Zoophysiology, Department of Biological Sciences, University of Aarhus, CF Møllers Alle 1131, DK 8000 Aarhus, Denmark
J Exp Biol 211:1057-62. 2008..The data demonstrate governing roles for lactate and proton ions in determining the temperature sensitivity of hemocyanin-O2 affinity in crustaceans...
- Hemoglobin function in deep-sea and hydrothermal-vent endemic fish: Symenchelis parasitica (Anguillidae) and Thermarces cerberus (Zoarcidae)Roy E Weber
Department of Zoophysiology, C F Møllers Alle, Building 131, University of Aarhus, DK 8000 Aarhus C, Denmark
J Exp Biol 206:2693-702. 2003..In the cathodic S. parasitica Hb, similar reduction appears to be associated with endothermic conformational changes that accompany the oxygenation reaction...
- Isohemoglobin differentiation in the bimodal-breathing amazon catfish Hoplosternum littoraleR E Weber
Department of Zoophysiology, University of Aarhus, DK 8000 Aarhus C, Denmark
J Biol Chem 275:17297-305. 2000....
- Lacking deoxygenation-linked interaction between cytoplasmic domain of band 3 and HbF from fetal red blood cellsR E Weber
Zoophysiology, Institute of Biological Sciences, University of Aarhus, Universitetsparken, Aarhus C, Denmark
Acta Physiol (Oxf) 191:247-52. 2007..g. via the major red cell membrane organising centre, band 3) between the individual oxygenation-linked reactions encountered in red cells...
- Functional adaptation and its molecular basis in vertebrate hemoglobins, neuroglobins and cytoglobinsRoy E Weber
Department of Zoophysiology, Institute of Biological Sciences, University of Aarhus, C F Møllers Alle 131, DK 8000 Aarhus C, Denmark
Respir Physiol Neurobiol 144:141-59. 2004..This review deals with the functional adaptations and the underlying molecular mechanisms in globins and presents case examples of molecular adaptations encountered in vertebrates and agnathans...
- 'Novel' factors that regulate oxygen binding in vertebrate hemoglobinsRoy E Weber
Zoophysiology Department, University of Aarhus, DK 8000 Aarhus C, Denmark
Micron 35:45-6. 2004
- Novel mechanism for high-altitude adaptation in hemoglobin of the Andean frog Telmatobius peruvianusRoy E Weber
Department of Zoophysiology, University of Aarhus, 131 C F Møllers Alle, DK 8000 Aarhus C, Denmark
Am J Physiol Regul Integr Comp Physiol 283:R1052-60. 2002..The findings are discussed in relation to other strategies for high-altitude adaptations in amphibians...
- ATP-induced temperature independence of hemoglobin-O2 affinity in heterothermic billfishRoy E Weber
Zoophysiology, Department of Biological Sciences, Aarhus University, Bygning 1131, C F Møllers Allé 3, DK 8000 Aarhus, Denmark
J Exp Biol 213:1579-85. 2010..The mechanism underlying this reduction in oxygenation enthalpy differs fundamentally from that in tuna, supporting independent evolution of this trait in these scombroid lineages...
- High-altitude adaptations in vertebrate hemoglobinsRoy E Weber
Zoophysiology, Institute of Biological Sciences, Building 1131, University of Aarhus, DK 8000 Aarhus, Denmark
Respir Physiol Neurobiol 158:132-42. 2007..This treatise reviews the molecular and cellular mechanisms that adapt haemoglobin-oxygen affinities in mammals, birds and ectothermic vertebrates at high altitude...
- Modulation of red cell glycolysis: interactions between vertebrate hemoglobins and cytoplasmic domains of band 3 red cell membrane proteinsRoy E Weber
Zoophysiology Department, Institute of Biological Sciences, University of Aarhus, DK 8000 Aarhus, Denmark
Am J Physiol Regul Integr Comp Physiol 287:R454-64. 2004....
- Nonvertebrate hemoglobins: functions and molecular adaptationsR E Weber
Danish Centre for Respiratory Adaptation, Department of Zoophysiology, Institute of Biology, University of Aarhus, Aarhus, Denmark
Physiol Rev 81:569-628. 2001..The divergent and convergent evolutionary trends evident in the structures and functions of nonvertebrate Hbs appear to be adaptive in extending the inhabitable environment available to Hb-containing organisms...
- Temperature dependence of haemoglobin-oxygen affinity in heterothermic vertebrates: mechanisms and biological significanceR E Weber
Zoophysiology, Institute of Biological Sciences, University of Aarhus, Aarhus, Denmark
Acta Physiol (Oxf) 202:549-62. 2011..and binding sites for the same effectors, indicating multiple evolutionary origins, but convergent physiological functionality (reductions in temperature dependence of O(2) -binding affinity that safeguard tissue O(2) supply)...
- Oxygen equilibria of cathodic eel hemoglobin analysed in terms of the MWC model and Adair's successive oxygenation theoryR J Feuerlein
Department of Zoophysiology, University of Aarhus, Denmark
J Comp Physiol B 165:597-606. 1996..A molecular mechanism for the reverse Bohr effect is suggested...
- Interaction between haemoglobin and synthetic peptides of the N-terminal cytoplasmic fragment of trout band 3 (AE1) proteinF B Jensen
Institute of Biology, Odense University, DK 5230 Odense M, Denmark
J Exp Biol 201:2685-90. 1998..In trout Hb IV, the presence of negatively charged Asp at position NA2 of the beta-chains (in contrast to positive or neutral residues in mammalian Hb) may weaken any interaction with the highly negatively charged peptides...
- Hydrogen ion titrations of the anodic and cathodic haemoglobin components of the European eel Anguilla anguillaC J Brauner
Danish Center for Respiratory Adaptation, Department of Zoophysiology, Aarhus University, Building 131, Denmark
J Exp Biol 201:2507-14. 1998..We conclude that the anodic component, which constitutes two-thirds of the haemoglobin in the eel, plays the predominant role in CO2 transport and pH homeostasis in vivo...
- ATP-induced reverse temperature effect in isohemoglobins from the endothermic porbeagle shark (Lamna nasus)Christina Larsen
Department of Zoophysiology, Institute of Biological Sciences, University of Aarhus, Building 131, 8000 Aarhus, Denmark
J Biol Chem 278:30741-7. 2003..The mechanism behind the reverse temperature effect resembles that found in the bluefin tuna (Thunnus thynnus), an endothermic teleost, thus evidencing further convergent evolution...
- Mutant hemoglobins (alpha 119-Ala and beta 55-Ser): functions related to high-altitude respiration in geeseR E Weber
Department of Zoophysiology, Aarhus University, Denmark
J Appl Physiol (1985) 75:2646-55. 1993..Adaptations securing Hb-O2 binding at extreme altitude are discussed...
- The anodic hemoglobin of Anguilla anguilla. Molecular basis for allosteric effects in a root-effect hemoglobinA Fago
Department of Zoophysiology, Institute of Biological Sciences, Building 131, University of Aarhus, 8000 Aarhus C, Denmark
J Biol Chem 272:15628-35. 1997....
- Oxygen binding by single red blood cells from the red-eared turtle Trachemys scriptaS Frische
Danish Centre for Respiratory Adaptation, Department of Zoophysiology, University of Aarhus, 8000 Aarhus C, Denmark
J Appl Physiol (1985) 90:1679-84. 2001..The considerable variation in oxygen affinity between individual red blood cells calls for its incorporation in models of tissue oxygenation...
- The archaeogastropod mollusc Haliotis iris: tissue and blood metabolites and allosteric regulation of haemocyanin functionJane W Behrens
Department of Zoophysiology, Institute Biological Sciences, University of Aarhus, DK 8000 Aarhus, Denmark
J Exp Biol 205:253-63. 2002....
- Allosteric effect of water in fish and human hemoglobinsChristian Hundahl
Department of Zoophysiology, Institute of Biological Sciences, C F Møllers Alle 131, University of Aarhus, DK 8000 Aarhus C, Denmark
J Biol Chem 278:42769-73. 2003..Addition of cofactors strongly increases the number of water molecules bound to eel hemoglobin A (as in human hemoglobin) but only weakly affects water binding to eel hemoglobin C...
- Effects of water activity on oxygen-binding in high-molecular weight, extracellular invertebrate hemoglobin and hemocyaninChristian Hundahl
Department of Zoophysiology, Institute of Biology, Building 131, University of Aarhus, Aarhus C DK 8000, Denmark
Comp Biochem Physiol B Biochem Mol Biol 136:83-90. 2003....
- Oxygen binding properties of non-mammalian nerve globinsChristian Hundahl
Zoophysiology, Institute of Biological Sciences, University of Aarhus, Denmark
FEBS J 273:1323-9. 2006..These results demonstrate a remarkable diversity in oxygenation characteristics of vertebrate and invertebrate nerve haemoglobins that clearly reflect distinct physiological roles...
- Functional properties of neuroglobin and cytoglobin. Insights into the ancestral physiological roles of globinsAngela Fago
Department of Zoophysiology, Institute of Biological Sciences, Aarhus University, Aarhus C, Denmark
IUBMB Life 56:689-96. 2004..The O2 binding properties of cytoglobin, which is upregulated upon hypoxia, are consistent with a role for this protein in O2-requiring reactions, such as those catalysed by hydroxylases...
- Allosteric regulation and temperature dependence of oxygen binding in human neuroglobin and cytoglobin. Molecular mechanisms and physiological significanceAngela Fago
Department of Zoophysiology, Institute of Biological Sciences, C F Møllers Alle 131, Aarhus University, DK 8000 Aarhus C, Denmark
J Biol Chem 279:44417-26. 2004..In contrast, the lower O2 affinity in NGB does not appear compatible with a physiological role involving mitochondrial O2 supply at the low O2 tensions found within neurons...
- Antagonistic interaction between oxygenation-linked lactate and CO2 binding to human hemoglobinMette Søby Nielsen
Zoophysiology, Department of Biological Sciences, University of Aarhus, DK8000 Aarhus, Denmark
Comp Biochem Physiol A Mol Integr Physiol 146:429-34. 2007....
- Effects of short-term hypoxia on neuroglobin levels and localization in mouse brain tissuesC Hundahl
Department of Zoophysiology, Institute of Biology, University of Aarhus, Denmark
Neuropathol Appl Neurobiol 31:610-7. 2005..The lack of Ngb up-regulation in the brain is consistent with results from previous long-term hypoxic experiments, suggesting that Ngb is not regulated by pure hypoxia in vivo...
- Water regulates oxygen binding in hagfish (Myxine glutinosa) hemoglobinGabriele Müller
Department of Zoophysiology, Institute of Biology, Building 131, University of Aarhus, DK 8000 Aarhus C, Denmark
J Exp Biol 206:1389-95. 2003..Our data show that water may act as an allosteric effector for hemoglobin within intact red cells and even in animals that do not experience large variations in blood osmolality...
- Reactivity studies of the Fe(III) and Fe(II)NO forms of human neuroglobin reveal a potential role against oxidative stressSusanna Herold
Laboratorium für Anorganische Chemie, Eidgenossische Technische Hochschule Honggerberg, CH 8093 Zurich, Switzerland
J Biol Chem 279:22841-7. 2004..Taken together, our data indicate that human neuroglobin may be an efficient scavenger of reactive oxidizing species and thus may play a role in the cellular defense against oxidative stress...
- Neuroglobin and cytoglobin in search of their role in the vertebrate globin familyThomas Hankeln
Institute of Molecular Genetics, Johannes Gutenberg University of Mainz, D 55099 Mainz, Germany
J Inorg Biochem 99:110-9. 2005..Cytoglobin is predominantly expressed in fibroblasts and related cell types, but also in distinct nerve cell populations. Much less is known about its function, although in fibroblasts it might be involved in collagen synthesis...
- Respiratory adaptations in a deep-sea orbiniid polychaete from Gulf of Mexico brine pool NR-1: metabolic rates and hemoglobin structure/function relationshipsStephane Hourdez
Department of Biology, 208 Mueller Lab, Pennsylvania State University, University Park, PA 16802, USA
J Exp Biol 205:1669-81. 2002..However, these polychaetes can withstand extended periods of anoxia both in the absence and presence of 1 mmol l(-1) sulfide (TL50=approx. 5.5 and 4 days, respectively)...
- Effects of magnesium and calcium on the oxygenation reaction of erythrocruorin from the marine polychaete Arenicola marina and the terrestrial oligochaete Lumbricus terrestrisTakehiko Ochiai
Department of Biology, Fukushima Medical University School of Medicine, Japan
Zoolog Sci 19:995-1000. 2002..These results reveal that Arenicola and Lumbricus belong to classes 1) and 3), respectively, and that the oxygenation function of both Ers may be controlled by effective utilization of the more dominant of the divalent cations Mg and Ca...
- Molecular and functional adaptations in deep-sea hemoglobinsStephane Hourdez
Equipe Ecophysiologie, CNRS UPMC UMR 7127, Station Biologique, 29680 Roscoff, France
J Inorg Biochem 99:130-41. 2005..These high affinities permit uptake of oxygen from hypoxic waters and the strong Bohr effects favor its release in the metabolizing acidic organs...
- Does altitudinal difference modulate the respiratory properties in subterranean rodents' (Cryptomys hottentotus mahali) blood?Marna S Broekman
Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa
Physiol Behav 88:77-81. 2006..The data indicate that the oxygen-transporting properties of mole-rat blood do not change markedly with increased elevation and that burrowing mammals are a priori hypoxia-adapted...
- Linked analysis of large cooperative, allosteric systems: the case of the giant HBL hemoglobinsNadja Hellmann
Institute for Molecular Biophysics, Johannes Gutenberg University, Mainz, Germany
Methods Enzymol 436:463-85. 2008..Possible advantages of a hierarchical cooperative model compared to a linear extension of the MWC model are discussed...
- Characterization of a globin-coupled oxygen sensor with a gene-regulating functionLiesbet Thijs
Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B 2610, Antwerp, Belgium
J Biol Chem 282:37325-40. 2007..Besides sensing ligands, in vitro data suggest that AvGReg(178) may have a role in O(2)-mediated NO-detoxification, yielding metAvGReg(178) and nitrate...
- Temperature alters the respiratory surface area of crucian carp Carassius carassius and goldfish Carassius auratusJørund Sollid
Department of Molecular Biosciences, University of Oslo, PO Box 1041, N 0316 Oslo, Norway
J Exp Biol 208:1109-16. 2005..As ambient oxygen and temperature can both induce the remodelling of the gills, the response appears primarily to be an adaptation to the oxygen demand of the fish...
- Nested allosteric interactions in extracellular hemoglobin of the leech Macrobdella decoraNadja Hellmann
Institute for Molecular Biophysics, University of Mainz, Jakob Welder Weg 26, 55128 Mainz, Germany
J Biol Chem 278:44355-60. 2003..Thus, besides hemocyanins and GroEL, the hexagonal bilayer hemoglobins represent another class of proteins in which the hierarchical quaternary structure provides the basis for nested interaction in their functional properties...
- Oxygen binding and its allosteric control in hemoglobin of the primitive branchiopod crustacean Triops cancriformisRalph Pirow
Institute of Zoophysiology, University of Munster, Germany
FEBS J 274:3374-91. 2007....
- Erythrocyte signal transduction pathways, their oxygenation dependence and functional significanceNadezhda N Barvitenko
I M Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg
Cell Physiol Biochem 15:1-18. 2005....