Steffen Thiel

Summary

Affiliation: University of Aarhus
Country: Denmark

Publications

  1. pmc Mannan-binding lectin (MBL)-associated serine protease-1 (MASP-1), a serine protease associated with humoral pattern-recognition molecules: normal and acute-phase levels in serum and stoichiometry of lectin pathway components
    S Thiel
    Department of Biomedicine, Aarhus University, Denmark
    Clin Exp Immunol 169:38-48. 2012
  2. pmc Mannan-binding lectin and procalcitonin measurement for prediction of postoperative infection
    Michael Siassi
    Department of Surgery, University Hospital Erlangen, Erlangen, Germany
    Crit Care 9:R483-9. 2005
  3. pmc Lack of the pattern recognition molecule mannose-binding lectin increases susceptibility to influenza A virus infection
    Wei Chuan Chang
    Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    BMC Immunol 11:64. 2010
  4. ncbi request reprint Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins and associated proteins
    Steffen Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Wilhelms Meyers Allé, 8000 Aarhus C, Denmark
    Mol Immunol 44:3875-88. 2007
  5. ncbi request reprint Clinical manifestations of mannan-binding lectin deficiency
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, DK8000 Denmark
    Mol Immunol 43:86-96. 2006
  6. ncbi request reprint Interaction of C1q and mannan-binding lectin (MBL) with C1r, C1s, MBL-associated serine proteases 1 and 2, and the MBL-associated protein MAp19
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Immunol 165:878-87. 2000
  7. ncbi request reprint Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Genes Immun 8:154-63. 2007
  8. doi request reprint Polymorphisms in mannan-binding lectin (MBL)-associated serine protease 2 affect stability, binding to MBL, and enzymatic activity
    Steffen Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Immunol 182:2939-47. 2009
  9. pmc M-ficolin binds selectively to the capsular polysaccharides of Streptococcus pneumoniae serotypes 19B and 19C and of a Streptococcus mitis strain
    Troels R Kjaer
    Department of Biomedicine, Aarhus University, Aarhus, Denmark
    Infect Immun 81:452-9. 2013
  10. ncbi request reprint Distinct pathways of mannan-binding lectin (MBL)- and C1-complex autoactivation revealed by reconstitution of MBL with recombinant MBL-associated serine protease-2
    T Vorup-Jensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Immunol 165:2093-100. 2000

Collaborators

Detail Information

Publications101 found, 100 shown here

  1. pmc Mannan-binding lectin (MBL)-associated serine protease-1 (MASP-1), a serine protease associated with humoral pattern-recognition molecules: normal and acute-phase levels in serum and stoichiometry of lectin pathway components
    S Thiel
    Department of Biomedicine, Aarhus University, Denmark
    Clin Exp Immunol 169:38-48. 2012
    ..The present data prepare the ground for studies on the associations of MASP-1 levels with disease...
  2. pmc Mannan-binding lectin and procalcitonin measurement for prediction of postoperative infection
    Michael Siassi
    Department of Surgery, University Hospital Erlangen, Erlangen, Germany
    Crit Care 9:R483-9. 2005
    ..Mannan-binding lectin (MBL) is a central molecule of the innate immune system and MBL deficiency is known to predispose to infection. Procalcitonin (PCT) is a sensitive marker for bacterial infection...
  3. pmc Lack of the pattern recognition molecule mannose-binding lectin increases susceptibility to influenza A virus infection
    Wei Chuan Chang
    Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    BMC Immunol 11:64. 2010
    ..These clinical observations were confirmed by animal model studies, in which mice genetically lacking MBL were susceptible to certain pathogens, including herpes simplex virus 2...
  4. ncbi request reprint Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins and associated proteins
    Steffen Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Wilhelms Meyers Allé, 8000 Aarhus C, Denmark
    Mol Immunol 44:3875-88. 2007
    ..A number of non-synonymous polymorphisms of the four recognition molecules and of the MASPs are known, and the implications of these alterations are being studied. The clinical impact of deficiencies will be discussed...
  5. ncbi request reprint Clinical manifestations of mannan-binding lectin deficiency
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, DK8000 Denmark
    Mol Immunol 43:86-96. 2006
    ..Ongoing research attempt to illuminate at which conditions MBL deficiency may lead to disease. With examples, this review illustrates the diversity of results obtained so far...
  6. ncbi request reprint Interaction of C1q and mannan-binding lectin (MBL) with C1r, C1s, MBL-associated serine proteases 1 and 2, and the MBL-associated protein MAp19
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Immunol 165:878-87. 2000
    ..Over 95% of the total MASPs and MAp19 found in serum are not complexed with MBL...
  7. ncbi request reprint Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Genes Immun 8:154-63. 2007
    ..The MASP-2 levels were reduced in individuals with p.V377A present. The MASP-2 present in individuals homozygous for p.377A or p.99Q had a normal enzyme activity whereas MASP-2 in individuals homozygous for p.126L was non-functional...
  8. doi request reprint Polymorphisms in mannan-binding lectin (MBL)-associated serine protease 2 affect stability, binding to MBL, and enzymatic activity
    Steffen Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Immunol 182:2939-47. 2009
    ..We find the R439H variant is common in Sub-Saharan Africans with a gene frequency of 10%. Our results indicate that individuals with different types of MASP-2 defects may be identified through genotyping...
  9. pmc M-ficolin binds selectively to the capsular polysaccharides of Streptococcus pneumoniae serotypes 19B and 19C and of a Streptococcus mitis strain
    Troels R Kjaer
    Department of Biomedicine, Aarhus University, Aarhus, Denmark
    Infect Immun 81:452-9. 2013
    ..In conclusion, we were able to demonstrate specific binding of M-ficolin to some capsular polysaccharides of the opportunistic pathogen S. pneumoniae and of the commensal bacterium S. mitis...
  10. ncbi request reprint Distinct pathways of mannan-binding lectin (MBL)- and C1-complex autoactivation revealed by reconstitution of MBL with recombinant MBL-associated serine protease-2
    T Vorup-Jensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Immunol 165:2093-100. 2000
    ..The results suggest that the catalytic activities of MASP-2 split between the two proteases of the C1 complex during the course of vertebrate complement evolution...
  11. ncbi request reprint Cooperation between MASP-1 and MASP-2 in the generation of C3 convertase through the MBL pathway
    Mette Møller-Kristensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Int Immunol 19:141-9. 2007
    ....
  12. doi request reprint MAp19, the alternative splice product of the MASP2 gene
    Søren E Degn
    Department of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, DK 8000 Aarhus C, Denmark
    J Immunol Methods 373:89-101. 2011
    ..Immunohistochemical analyses combined with qRT-PCR revealed that both MAp19 and MASP-2 were mainly expressed in hepatocytes. High levels of MAp19 were found in urine, where MASP-2 was absent...
  13. pmc Structural basis for activation of the complement system by component C4 cleavage
    Rune T Kidmose
    Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
    Proc Natl Acad Sci U S A 109:15425-30. 2012
    ..Our results provide a unified structural framework for understanding the early and essential step of C4 cleavage in the elimination of pathogens and altered self through two major pathways of complement activation...
  14. doi request reprint Investigations on the pattern recognition molecule M-ficolin: quantitative aspects of bacterial binding and leukocyte association
    Troels R Kjaer
    Aarhus University, Wilhelm Meyers Allé 4, DK 8000 Aarhus C, Denmark
    J Leukoc Biol 90:425-37. 2011
    ..Reported M-ficolin expression by type II pneumocytes could not be verified. We demonstrate the specific binding of M-ficolin to sialic acids in the capsule of GBS and give quantitative aspects of the cell-associated M-ficolin...
  15. pmc Mannan-binding lectin deficiency modulates the humoral immune response dependent on the genetic environment
    Marieta Ruseva
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Immunology 127:279-88. 2009
    ..These findings indicate that the modifying effect of MBL on the humoral immune response is influenced by the genetic environment...
  16. doi request reprint MAp44, a human protein associated with pattern recognition molecules of the complement system and regulating the lectin pathway of complement activation
    Søren E Degn
    Departments of Medical Microbiology and Immunology, University of Aarhus, Arhus, Denmark
    J Immunol 183:7371-8. 2009
    ..MAp44 competes with MASP-2 for binding to MBL and ficolins, resulting in inhibition of complement activation. Our results add a novel mechanism to those known to control the innate immune system...
  17. doi request reprint Toward a structure-based comprehension of the lectin pathway of complement
    Troels R Kjaer
    Department of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, DK 8000 Aarhus, Denmark
    Mol Immunol 56:413-22. 2013
    ..These models are used to discuss cis- versus trans-activation of MASP proteases and the geometry of C4 deposition occurring on glycans in the lectin pathway. ..
  18. doi request reprint Co-complexes of MASP-1 and MASP-2 associated with the soluble pattern-recognition molecules drive lectin pathway activation in a manner inhibitable by MAp44
    Søren E Degn
    Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark
    J Immunol 191:1334-45. 2013
    ..We present support for this contention...
  19. pmc Mitochondria and the lectin pathway of complement
    Christel R Brinkmann
    Department of Biomedicine, Faculty of Health Sciences, Aarhus University, DK 8000 Aarhus C, Denmark
    J Biol Chem 288:8016-27. 2013
    ..Thus, we report here the first indication of involvement of the lectin pathway in mitochondrial immune handling...
  20. pmc Studies of the pattern recognition molecule H-ficolin: specificity and purification
    Rikke M Zacho
    Department of Medical Microbiology and Immunology, Aarhus University, 8000 Aarhus, Denmark
    J Biol Chem 287:8071-81. 2012
    ..This study extends our knowledge of the specificity of this pattern recognition molecule, and the purified product will enable further studies...
  21. doi request reprint Biological variations of MASP-3 and MAp44, two splice products of the MASP1 gene involved in regulation of the complement system
    Søren E Degn
    Department of Medical Microbiology and Immunology, Aarhus University, Wilhelm Meyers Allé 4, 8000 Arhus C, Denmark
    J Immunol Methods 361:37-50. 2010
    ..Thus, neither of the two proteins behaves as a classical acute phase protein...
  22. ncbi request reprint Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses
    H Liu
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Scand J Immunol 53:489-97. 2001
    ..In comparison, serum amyloid A component (SAA) peaked at 15 h with an approximate 100-fold increase...
  23. pmc Non-synonymous polymorphisms in the FCN1 gene determine ligand-binding ability and serum levels of M-ficolin
    Christian Gytz Ammitzbøll
    Department of Rheumatology, Aarhus University Hospital, Aarhus, Denmark
    PLoS ONE 7:e50585. 2012
    ..Our aim was to explore associations between SNPs in FCN1, encoding M-ficolin and corresponding protein concentrations, and the impact of non-synonymous SNPs on protein function...
  24. doi request reprint Mannan-binding lectin-associated serine protease (MASP)-1 is crucial for lectin pathway activation in human serum, whereas neither MASP-1 nor MASP-3 is required for alternative pathway function
    Søren E Degn
    Department of Biomedicine, Aarhus University, DK 8000 Aarhus C, Denmark
    J Immunol 189:3957-69. 2012
    ..Hence, in functional terms, it appears that MASP-1 and MASP-2 act in a manner analogous to that of C1r and C1s of the classical pathway...
  25. ncbi request reprint Mannan-binding lectin--a soluble pattern recognition molecule
    Mihaela Gadjeva
    Department of Medical Microbiology and Immunology, University of Aarhus, The Bartholin Building, 8000 Aarhus, DK, Denmark
    Mol Immunol 41:113-21. 2004
    ..MBL has a potential to react against altered-self structures, as found on apoptotic cells, cancers or ischemic-reperfused tissue. The focus of the current review is to summarize the recent progress in our understanding of MBL functions...
  26. ncbi request reprint Mannan-binding-lectin-associated serine proteases, characteristics and disease associations
    Rikke Sørensen
    Department of Medical Microbiology and Immunology, Wilhelm Meyers Alle, University of Aarhus, 8000 Aarhus, Denmark
    Springer Semin Immunopathol 27:299-319. 2005
    ..The gene frequency of the mutation among Caucasians is 3.6%. It is not found in Chinese, who present a different mutation also associated with MASP-2 deficiency...
  27. ncbi request reprint Purification and characterization of two mannan-binding lectins from mouse serum
    S Hansen
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    J Immunol 164:2610-8. 2000
    ..Serum concentrations of mMBL-A in laboratory strains and wild mice were found to vary from 5 to 80 microg/ml, with wild mice tending to show higher levels than laboratory strains...
  28. ncbi request reprint A second serine protease associated with mannan-binding lectin that activates complement
    S Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Nature 386:506-10. 1997
    ..Thus complement activation through MBL, like the classical pathway, involves two serine proteases and may antedate the development of the specific immune system of vertebrates...
  29. ncbi request reprint Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals
    Mette Møller-Kristensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus C 8000, Denmark
    J Immunol Methods 282:159-67. 2003
    ..Gel permeation chromatography (GPC) indicated that all MASP-2 in serum is present in complexes with MBL and ficolins...
  30. pmc Investigations on collectin liver 1
    Esben Axelgaard
    Department of Biomedicine, Wilhelm Meyers Allé 4, Faculty of Health Sciences, Aarhus University, 8000 Aarhus C, Denmark
    J Biol Chem 288:23407-20. 2013
    ..The presented data establish a basis for future studies on the biological role of CL-L1. ..
  31. ncbi request reprint Elevated levels of mannan-binding lectin in patients with type 1 diabetes
    Troels Krarup Hansen
    Medical Department M, Aarhus University Hospital, Aarhus University, DK 8000 Aarhus, Denmark
    J Clin Endocrinol Metab 88:4857-61. 2003
    ..036). In conclusion, our data demonstrate that circulating MBL concentrations are significantly elevated in patients with type 1 diabetes and suggest a possible role of MBL in the pathogenesis of renovascular complications in diabetes...
  32. doi request reprint The role of nanometer-scaled ligand patterns in polyvalent binding by large mannan-binding lectin oligomers
    Louise C Gjelstrup
    Biophysical Immunology Laboratory, Aarhus University, DK 8000 Aarhus C, Denmark
    J Immunol 188:1292-306. 2012
    ....
  33. ncbi request reprint Chemotherapy-related infections in patients with multiple myeloma: associations with mannan-binding lectin genotypes
    Ingolf Mølle
    Department of Haematology, University Hospital of Aarhus, THG, Aarhus, Denmark
    Eur J Haematol 77:19-26. 2006
    ..To study a possible association between mannan-binding lectin genotypes and severe infections in patients with multiple myeloma receiving moderate strength induction chemotherapy...
  34. doi request reprint Assays for the mannan-binding lectin pathway
    Mihaela Gadjeva
    University of Aarhus, Aarhus, Denmark
    Curr Protoc Immunol . 2004
    ..The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway...
  35. pmc Disease-causing mutations in genes of the complement system
    Søren E Degn
    Department of Medical Microbiology and Immunology, Aarhus University, Wilhelm Meyers Allé 4, 8000 Aarhus C, DK Denmark
    Am J Hum Genet 88:689-705. 2011
    ..Here, we compare the functional immunologic consequences of "conventional" complement deficiencies with these newly described developmental roles...
  36. doi request reprint Assay interference caused by antibodies reacting with rat kappa light-chain in human sera
    Søren E Degn
    Department of Medical Microbiology and Immunology, Aarhus University, Wilhelm Meyers Allé 4, DK 8000 Aarhus C, Denmark
    J Immunol Methods 372:204-8. 2011
    ..Our results highlight a major pitfall of potential relevance to many sandwich-type assays, as well as an approach to rectify such problems...
  37. ncbi request reprint Quantification of mannan-binding lectin
    Pernille D Frederiksen
    Department of Medical Microbiology and Immunology, University of Aarhus, Wilhelm Meyers Alle, 8000 Aarhus, DK, Denmark
    J Immunol Methods 315:49-60. 2006
    ..Titrations of recombinant MBL served as standard. In sera from homozygous mutants (O/O) the MBL concentrations estimated on Western blot were in the range of 100 to 500 ng/ml and correlated with that measured in the 93C-based TRIFMA...
  38. pmc Effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, L-ficolin, and H-ficolin
    Anders Krarup
    Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK 8000 Aarhus C, Denmark
    Infect Immun 73:1052-60. 2005
    ..The concentrations of the three proteins in 97 plasma samples were estimated. The median concentrations were 0.8 mug per ml for MBL, 3.3 mug per ml for L-ficolin, and 18.4 mug per ml for H-ficolin...
  39. ncbi request reprint Humoral pattern recognition molecules: mannan-binding lectin and ficolins
    Steffen Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Adv Exp Med Biol 653:58-73. 2009
    ..It appears that MBL deficiency may modulate the prognosis of inflammatory and autoimmune diseases. What is known about the mechanisms behind this broad scope of activities of MBL and ficolins is discussed in this chapter...
  40. ncbi request reprint L-ficolin is a pattern recognition molecule specific for acetyl groups
    Anders Krarup
    Institute of Medical Microbiology and Immunology, University of Aarhus, 8000 Aarhus, Denmark
    J Biol Chem 279:47513-9. 2004
    ..The column was loaded in the presence of EDTA and high salt, and L-ficolin was eluted by decreasing the salt concentration. Further purification was achieved by ion exchange chromatography...
  41. ncbi request reprint MASP-3 and its association with distinct complexes of the mannan-binding lectin complement activation pathway
    M R Dahl
    Department of Medical Microbiology and Immunology, University of Aarhus, DK 8000, Aarhus, Denmark
    Immunity 15:127-35. 2001
    ..MASP-1, MAp19, and direct C3-cleaving activity are associated with smaller oligomers whereas MASP-3 is found together with MASP-2 on larger oligomers. MASP-3 downregulate the C4 and C2 cleaving activity of MASP-2...
  42. ncbi request reprint MASP-2, the C3 convertase generating protease of the MBLectin complement activating pathway
    T Vorup-Jensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Immunobiology 199:348-57. 1998
    ..Phylogenetic analysis indicates that MASP-2 is closer to C1r and C1s than is MASP-1, but no particular association between MASP-2 and the C4 cleaving enzyme, C1s, can be deduced from sequence comparison...
  43. pmc Polymorphisms in the MASP1 gene are associated with serum levels of MASP-1, MASP-3, and MAp44
    Christian Gytz Ammitzbøll
    Department of Rheumatology, Aarhus University Hospital, Aarhus, Denmark
    PLoS ONE 8:e73317. 2013
    ..Our aim was to explore associations between polymorphisms in MASP1 and corresponding concentrations of MASP-1, MASP-3, and MAp44 in plasma as well as the genetic contribution to the equilibrium between the three proteins...
  44. doi request reprint Recombinant expression of the autocatalytic complement protease MASP-1 is crucially dependent on co-expression with its inhibitor, C1 inhibitor
    Søren E Degn
    Department of Biomedicine, Aarhus University, DK 8000 Aarhus C, Denmark
    Protein Expr Purif 88:173-82. 2013
    ..We have generalized the concept to the expression also of MASP-2 entirely in its zymogen form and with improved yields. We suggest a general advantage of expressing aggressive, autocatalytic proteases with their cognate inhibitors...
  45. pmc Mannan-binding lectin in diabetic kidney disease: the impact of mouse genetics in a type 1 diabetes model
    Jakob Appel Østergaard
    Department of Endocrinology and Internal Medicine, Aarhus University Hospital and The Medical Research Laboratories, Institute of Clinical Medicine, Faculty of Health Sciences, Aarhus University, 8000 Aarhus C, Denmark
    Exp Diabetes Res 2012:678381. 2012
    ..However, this involvement may differ among the animal strains. We thus examined the impact of the genetic background on the role of MBL in diabetic nephropathy...
  46. doi request reprint Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma
    Thomas Wittenborn
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    J Innate Immun 2:167-80. 2010
    ..We suggest that M-ficolin should also be considered as a humoral pattern recognition molecule...
  47. doi request reprint Shedding of large functionally active CD11/CD18 Integrin complexes from leukocyte membranes during synovial inflammation distinguishes three types of arthritis through differential epitope exposure
    Louise Carstensen Gjelstrup
    Biophysical Immunology Laboratory, Aarhus University, Aarhus, Denmark
    J Immunol 185:4154-68. 2010
    ..As reported here for synovial inflammation changes in the concentration or structure of these complexes should be considered as likely contributors to disease activity...
  48. ncbi request reprint New perspectives on mannan-binding lectin-mediated complement activation
    Søren E Degn
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Immunobiology 212:301-11. 2007
    ..The emerging picture of the complement system is more that of a small "scale-free" network where C3 acts as the main hub, than that of three linear pathways converging in a common terminal pathway...
  49. ncbi request reprint Conformational changes in mannan-binding lectin bound to ligand surfaces
    Mingdong Dong
    Interdisciplinary Nanoscience Center iNANO, Department of Physiucs and Astronomy, University of Aarhus, Aarhus C, Denmark
    J Immunol 178:3016-22. 2007
    ....
  50. ncbi request reprint Assays for the functional activity of the mannan-binding lectin pathway of complement activation
    Steffen Thiel
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Immunobiology 205:446-54. 2002
    ..In parallel we determine the MBL concentration in the sample. As predicted a very high correlation is observed between the results of the two assays...
  51. ncbi request reprint The mannan-binding lectin pathway of complement activation: biology and disease association
    S V Petersen
    Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK 8000 Aarhus, Denmark
    Mol Immunol 38:133-49. 2001
    ..In this review, we focus on the structure and function of the proteins within the MBL pathway and address the properties of the pathway as an initiator of the host response against potential pathogenic micro-organisms...
  52. ncbi request reprint Complement activation upon binding of mannan-binding protein to HIV envelope glycoproteins
    J S Haurum
    Department of Immunology, University of Aarhus, Denmark
    AIDS 7:1307-13. 1993
    ..Retroviruses can activate the complement system in the absence of antibodies, and the purpose of this study was to examine whether the serum collection, mannan-binding protein (MBP), could mediate such complement activation...
  53. ncbi request reprint M-ficolin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement
    P D Frederiksen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, DK, Denmark
    Scand J Immunol 62:462-73. 2005
    ..We developed a monoclonal rat anti-human-M/L-ficolin antibody and verified by flow cytometric analysis the presence of ficolin on the surface of peripheral blood monocytes...
  54. ncbi request reprint On the site of C4 deposition upon complement activation via the mannan-binding lectin pathway or the classical pathway
    M Møller-Kristensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Scand J Immunol 57:556-61. 2003
    ..More than 99% of deposited C4b was bound directly to the solid phase rather than to the initiator complex. Our approach cannot answer the question of the whereabouts of the C2 when it is cleaved...
  55. ncbi request reprint Control of the classical and the MBL pathway of complement activation
    S V Petersen
    Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK 8000, Aarhus, Denmark
    Mol Immunol 37:803-11. 2000
    ..The results in the present report suggests the possibility of specifically inhibiting of the MBL pathway of complement activation...
  56. ncbi request reprint Recombinant expression of human mannan-binding lectin
    T Vorup-Jensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Int Immunopharmacol 1:677-87. 2001
    ..We report that rMBL structurally and functionally similar to natural MBL can be obtained through synthesis in the human embryonic kidney cells followed by selective carbohydrate affinity chromatography...
  57. pmc Mannan-binding lectin modulates the response to HSV-2 infection
    M Gadjeva
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Clin Exp Immunol 138:304-11. 2004
    ..0369). This suggests that lack of MBL-mediated complement activation increases susceptibility to viral infection...
  58. pmc Cloning and sequencing of a cDNA encoding chicken mannan-binding lectin (MBL) and comparison with mammalian analogues
    S B Laursen
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Immunology 93:421-30. 1998
    ..The phylogenetic analysis indicates that the gene duplication leading to two different MBL forms in mammals occurred after the split from birds and reptiles. This concurs with the finding of only one form of MBL in chickens...
  59. ncbi request reprint Mannan-binding lectin recognizes structures on ischaemic reperfused mouse kidneys and is implicated in tissue injury
    M Møller-Kristensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Scand J Immunol 61:426-34. 2005
    ..Our data implicate a role of MBL in I/R-induced kidney injury...
  60. doi request reprint M-ficolin levels reflect disease activity and predict remission in early rheumatoid arthritis
    Christian G Ammitzbøll
    Aarhus University Hospital, Aarhus, Denmark
    Arthritis Rheum 65:3045-50. 2013
    ....
  61. doi request reprint Toward a structure-based comprehension of the lectin pathway of complement
    Troels R Kjaer
    Department of Biomedicine, Aarhus University, Aarhus, Denmark
    Mol Immunol 56:222-31. 2013
    ..These models are used to discuss cis- versus trans-activation of MASP proteases and the geometry of C4 deposition occurring on glycans in the lectin pathway. ..
  62. ncbi request reprint Mannose-binding lectin and mortality in type 2 diabetes
    Troels Krarup Hansen
    Immunoendocrine Research Unit, Medical Department M and Medical Research Laboratories, Aarhus University Hospital, Aarhus, Denmark
    Arch Intern Med 166:2007-13. 2006
    ..We evaluated the relationship between serum MBL and mortality and development of albuminuria in type 2 diabetes...
  63. ncbi request reprint Gene-environment interactions in multiple sclerosis: innate and adaptive immune responses to human endogenous retrovirus and herpesvirus antigens and the lectin complement activation pathway
    Tove Christensen
    Institute of Medical Microbiology and Immunology, Bartholin Building, University of Aarhus, and Department of Neurology, Aarhus University Hospital, Denmark
    J Neuroimmunol 183:175-88. 2007
    ..Our results support that HERV-H and the antiviral immune response may play a role in MS development, and also underline the tenuous nature of specific genetic contributions to this complex disease...
  64. ncbi request reprint Recombinant mannan-binding lectin (MBL) for therapy
    J C Jensenius
    Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
    Biochem Soc Trans 31:763-7. 2003
    ..A transfected human cell line produces MBL showing the same biological activity as plasma-derived MBL, and an essentially identical profile on MS. The production has been scaled up and clinical trials will start this year...
  65. ncbi request reprint An assay for the mannan-binding lectin pathway of complement activation
    S V Petersen
    Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000, Aarhus, Denmark
    J Immunol Methods 257:107-16. 2001
    ..The assay permits for the determination of MBL complex activity in serum and plasma samples and may thus be used to evaluate the clinical implications of complement activation via this pathway...
  66. ncbi request reprint MBL2 polymorphism and risk of severe infections in multiple myeloma patients receiving high-dose melphalan and autologous stem cell transplantation
    I Mølle
    Department of Haematology, University Hospital of Aarhus, THG, Aarhus, Denmark
    Bone Marrow Transplant 38:555-60. 2006
    ..20 (95% CI: 0.52-2.78), (P=0.67). The findings indicate that MBL to some extent protects against the most severe infections during ASCT...
  67. doi request reprint Protein-fatty acid complexes: biochemistry, biophysics and function
    Christel R Brinkmann
    Department of Biomedicine, Faculty of Health Sciences, Aarhus University, Denmark
    FEBS J 280:1733-49. 2013
    ..Further studies will aim to determine how the molecular properties of the different protein-lipid complexes correlate with their biological efficacy...
  68. ncbi request reprint Association between mannose-binding lectin and vascular complications in type 1 diabetes
    Troels K Hansen
    Immunoendocrine Research Unit, Medical Department M Endocrinology and Diabetes, Aarhus University Hospital, Norrebrogade 42 44, DK 8000 Aarhus C, Denmark
    Diabetes 53:1570-6. 2004
    ....
  69. doi request reprint Effects of interferon-beta therapy on innate and adaptive immune responses to the human endogenous retroviruses HERV-H and HERV-W, cytokine production, and the lectin complement activation pathway in multiple sclerosis
    Thor Petersen
    Department of Neurology, Aarhus University Hospital, Nørrebrogade 44, DK 8000 Aarhus C, Denmark
    J Neuroimmunol 215:108-16. 2009
    ..We found no overall changes in Th1/Th2 ratios. Our results support that HERV-H/HERV-W and the antiviral immune response may play a role in MS development, and that these HERVs have potential as biomarkers for disease activity...
  70. ncbi request reprint Tail-vein injection of mannan-binding lectin DNA leads to high expression levels of multimeric protein in liver
    T Vorup-Jensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus C, 8000, Denmark
    Mol Ther 3:867-74. 2001
    ..Thus, liver expression following injection of naked DNA is an alternative to reconstitution therapy with a protein having a complex quaternary structure...
  71. pmc Hormonal regulation of mannan-binding lectin synthesis in hepatocytes
    C M Sørensen
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Clin Exp Immunol 145:173-82. 2006
    ..The results suggest that MBL synthesis in humans may be increased by thyroid hormone and GH, whereas it does not exhibit a classical IL-6-dependent response...
  72. doi request reprint Assay for estimation of the functional activity of the mannan-binding lectin pathway of the complement system
    Troels R Kjaer
    Department of Biomedicine, Aarhus University, Aarhus, Denmark
    Methods Mol Biol 1100:131-9. 2014
    ..Here we describe a functional assay for estimation of MBL pathway activity by detection of complement factor deposition onto microtiter plate wells coated with a physiological relevant ligand for MBL. ..
  73. ncbi request reprint Intensive insulin therapy exerts antiinflammatory effects in critically ill patients and counteracts the adverse effect of low mannose-binding lectin levels
    Troels Krarup Hansen
    Medical Department M Endocrinology and Diabetes, Aarhus University Hospital, Aarhus, Denmark
    J Clin Endocrinol Metab 88:1082-8. 2003
    ..Possible adverse effects of low baseline MBL are overcome by intensive insulin therapy...
  74. ncbi request reprint Umbilical cord mannan-binding lectin and infections in early childhood
    S Kielgast
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
    Scand J Immunol 57:167-72. 2003
    ..The HR of hospitalization for viral infections was 2.8 (CI, 1.3-5.9). Low levels of MBL in UCB were associated with hospitalization in general and particularly with hospitalization for viral infections...
  75. ncbi request reprint Mannan-binding lectin and RSV lower respiratory tract infection leading to hospitalization in children: a case-control study from Soweto, South Africa
    I A Kristensen
    The Danish Epidemiology Science Centre, University of Aarhus, Aarhus, Denmark
    Scand J Immunol 60:184-8. 2004
    ..00 (CI 0.99-1.03) and OR 1.24 (0.73-2.12). We did not find support for the hypothesis that MBL deficiency leads to the hospitalization for LRTI caused by RSV...
  76. ncbi request reprint Collections and ficolins: humoral lectins of the innate immune defense
    Uffe Holmskov
    Department of Medical Biology, University of Southern Denmark, DK5000, Odeuse, Denmark
    Annu Rev Immunol 21:547-78. 2003
    ..Deficiencies of the proteins may predispose to infections or other complications, e.g., reperfusion injuries or autoimmune diseases. Structure, function, clinical implications, and phylogeny are reviewed...
  77. ncbi request reprint Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G
    Mikio Kuraya
    Department of Biochemistry, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960 1295, Japan
    Int Immunol 15:109-17. 2003
    ..Since the glioma cells used are derived from astrocytes, this suggests that human astrocytes may be a source of some components of the LCP in the brain...
  78. pmc C-reactive protein collaborates with plasma lectins to boost immune response against bacteria
    Patricia M L Ng
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    EMBO J 26:3431-40. 2007
    ..We propose that CRP does not act alone but collaborates with other plasma PRRs to form stable pathogen recognition complexes when targeting a wide range of bacteria for destruction...
  79. ncbi request reprint The mannan-binding lectin-associated serine proteases (MASPs) and MAp19: four components of the lectin pathway activation complex encoded by two genes
    Wilhelm Schwaeble
    Department of Microbiology and Immunology, University of Leicester, England, UK
    Immunobiology 205:455-66. 2002
    ..MASP-2 is the protease responsible for activating C4 and C2 to generate the C3 convertase, C4bC2b. The biological function of the remaining three proteins has not yet been resolved...
  80. ncbi request reprint Genetic influences on mannan-binding lectin (MBL) and mannan-binding lectin associated serine protease-2 (MASP-2) activity
    Grith L Sorensen
    Medical Biotechnology Center, University of Southern Denmark, Odense, Denmark
    Genet Epidemiol 31:31-41. 2007
    ..34 [0.25;0.42]. The data indicate a strong genetic influence for the serum levels of MBL and for MASP-2 activity with a significant genetic correlation between the two traits...
  81. pmc Mannan-binding lectin activates C3 and the alternative complement pathway without involvement of C2
    Barbro Selander
    Institute of Laboratory Medicine, Section of Microbiology, Immunology, and Glycobiology, Lund University, Lund, Sweden
    J Clin Invest 116:1425-34. 2006
    ..MBL-dependent C2 bypass activation could be particularly important in various inherited and acquired complement deficiency states...
  82. ncbi request reprint Activation of the lectin pathway by natural IgM in a model of ischemia/reperfusion injury
    Ming Zhang
    CBR Institute of Biomedical Research Inc, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
    J Immunol 177:4727-34. 2006
    ..The results reveal that IgM binds initially to ischemic Ag providing a binding site for mannan-binding lectin which subsequently leads to activation of complement and injury...
  83. ncbi request reprint Identification of the site of human mannan-binding lectin involved in the interaction with its partner serine proteases: the essential role of Lys55
    Florence Teillet
    Laboratoire d Enzymologie Moleculaire, Institut de Biologie Structurale Jean Pierre Ebel, 41 rue Jules Horowitz, Grenoble, France
    J Immunol 178:5710-6. 2007
    ..The binding sites for MASP-2/MAp19 and MASP-1/3 have common features but are not strictly identical...
  84. ncbi request reprint Serum mannan-binding lectin-associated serine protease 2 levels in colorectal cancer: relation to recurrence and mortality
    Henriette Ytting
    Department of Surgical Gastroenterology, Hvidovre University Hospital, DK 2650 Hvidovre, Denmark
    Clin Cancer Res 11:1441-6. 2005
    ..The level of MASP-2 is genetically determined, and the aim of the present study was to evaluate the effect of MASP-2 levels on postoperative infection, recurrence and survival...
  85. ncbi request reprint Studies on the mechanisms of allergen-induced activation of the classical and lectin pathways of complement
    Lilian Varga
    3rd Department of Medicine, Faculty of Medicine, Semmelweis University, H 1125 Budapest, Kútvölgyi Str 4, Hungary
    Mol Immunol 39:839-46. 2003
    ..Depending on the allergens, this activation may be initiated through C1, MBL, or both...
  86. ncbi request reprint Mannose-binding lectin as a predictor of microalbuminuria in type 1 diabetes: an inception cohort study
    Peter Hovind
    Steno Diabetes Center, Niels Steensens Vej 2, DK 2820 Gentofte, Denmark
    Diabetes 54:1523-7. 2005
    ....
  87. ncbi request reprint Inherited deficiency of mannan-binding lectin-associated serine protease 2
    Kristian Stengaard-Pedersen
    Department of Rheumatology, Aarhus University Hospital, Denmark
    N Engl J Med 349:554-60. 2003
  88. ncbi request reprint L-MBP is expressed in epithelial cells of mouse small intestine
    Kazuhide Uemura
    Department of Biological Chemistry, Core Research for Science and Technology Project, Japan Science and Technology Corp, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
    J Immunol 169:6945-50. 2002
    ..These findings suggest that L-MBP plays a role in mucosal innate immunity...
  89. doi request reprint Binding and activation of human and mouse complement by Cryptosporidium parvum (Apicomplexa) and susceptibility of C1q- and MBL-deficient mice to infection
    Franz Petry
    Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University Mainz, Augustusplatz Hochhaus, D 55101 Mainz, Germany
    Mol Immunol 45:3392-400. 2008
    ..The contribution of the complement system and the lectin pathway in particular to the host defence against cryptosporidiosis may become apparent in situations of immunodeficiency such as HIV infections or in early childhood...
  90. ncbi request reprint Preoperative mannan-binding lectin pathway and prognosis in colorectal cancer
    Henriette Ytting
    Department of Surgical Gastroenterology 435, Hvidovre University Hospital, Kettegard Alle 30, 2650 Hvidovre, Denmark
    Cancer Immunol Immunother 54:265-72. 2005
    ....
  91. ncbi request reprint Phase I safety, tolerability, and pharmacokinetic study of recombinant human mannan-binding lectin
    Kenneth Ahrend Petersen
    NatImmune A S, Fruebjergvej 3, Box 3 DK 2100, Copenhagen, Denmark
    J Clin Immunol 26:465-75. 2006
    ..In conclusion, no safety or tolerability concern was raised following rhMBL administration no signs of immunogenicity detected, and an rhMBL plasma level judged sufficient to achieve therapeutic benefit (>1000 ng/mL) can be achieved...
  92. ncbi request reprint Complement activation and diabetic vascular complications
    Jakob Østergaard
    The Medical Research Laboratories, Clinical Institute, Medical Department M Diabetes and Endocrinology, Aarhus University Hospital, Nørrebrogade 44, DK 8000 Aarhus C, Denmark
    Clin Chim Acta 361:10-9. 2005
    ..Several ways of specifically manipulating the complement system already exist. However, whether or not these drugs provide new targets for intervention on diabetic vascular complications is still unknown...
  93. pmc Deficiency of mannose-binding lectin greatly increases susceptibility to postburn infection with Pseudomonas aeruginosa
    Mette Møller-Kristensen
    Laboratory of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Immunol 176:1769-75. 2006
    ..aeruginosa infection following burn injury and suggests that MBL deficiency in humans maybe a premorbid variable in the predisposition to infection in burn victims...
  94. pmc Mannose-binding lectin-deficient mice are susceptible to infection with Staphylococcus aureus
    Lei Shi
    Laboratory of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, JRG 1402, Boston, MA 02114, USA
    J Exp Med 199:1379-90. 2004
    ..aureus infection in mice and raises the idea that the MBL gene may act as a disease susceptibility gene against staphylococci infections in humans...
  95. ncbi request reprint Plasma mannan-binding lectin levels and activity are increased in allergic patients
    Savneet Kaur
    J Allergy Clin Immunol 116:1381-3. 2005
  96. ncbi request reprint Thyroid hormone increases mannan-binding lectin levels
    Anne Lene Dalkjaer Riis
    Medical Department M, Aarhus University Hospital, Denmark
    Eur J Endocrinol 153:643-9. 2005
    ..Mannan-binding lectin (MBL), a protein of the innate immune system, may constitute a connection between these fields...
  97. ncbi request reprint Mannan-binding lectin in asthma and allergy
    Savneet Kaur
    Department of Molecular Biochemistry and Diagnostics, Institute of Genomics and Integrative Biology, Mall Road, Delhi 100 007, India
    Curr Allergy Asthma Rep 6:377-83. 2006
    ..In this review, we summarize and explore this potential and a possible novel role of MBL in asthma and allergy...
  98. ncbi request reprint Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis
    Kazue Takahashi
    Department of Pediatrics, Laboratory of Developmental Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Microbes Infect 4:773-84. 2002
    ..Our results indicate that MBL-A is a modulator of inflammation in vivo and in vitro in the mouse and that the role of MBL may extend beyond its role as an opsonin...
  99. ncbi request reprint The effects of GH and hormone replacement therapy on serum concentrations of mannan-binding lectin, surfactant protein D and vitamin D binding protein in Turner syndrome
    Claus Højbjerg Gravholt
    Medical Department M Endocrinology and Diabetes and Medical Research Laboratories, Arhus Kommunehospital, Arhus University Hospital, 8000 Arhus C, Denmark
    Eur J Endocrinol 150:355-62. 2004
    ....
  100. ncbi request reprint An enzyme-linked immunosorbent assay (ELISA) for quantification of mouse surfactant protein D (SP-D)
    Soren Hansen
    Department of Immunology and Microbiology, University of Southern Denmark, Winsloewparken 21 1, Odense, Denmark
    J Immunol Methods 330:75-85. 2008
    ..8 ng/ml). With this ELISA, it will be possible to study the regulation of SP-D in various mouse models and upon various stimuli...
  101. pmc Burn injury reveals altered phenotype in mannan-binding lectin-deficient mice
    Mette Møller-Kristensen
    Laboratory of Developmental Immunology, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    J Invest Dermatol 127:1524-31. 2007
    ..Mice lacking C1q, C4, or C3 did not show the lack of eschar separation seen in MBL null-burn phenotype. These findings implicate MBL as an important molecule in the maintenance of the homeostatic balance...