Søren J Sørensen

Summary

Affiliation: University of Copenhagen
Country: Denmark

Publications

  1. ncbi request reprint Studying plasmid horizontal transfer in situ: a critical review
    Søren J Sørensen
    Department of Microbiology, Institute of Biology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Nat Rev Microbiol 3:700-10. 2005
  2. ncbi request reprint Making bio-sense of toxicity: new developments in whole-cell biosensors
    Søren J Sørensen
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Curr Opin Biotechnol 17:11-6. 2006
  3. pmc In vivo detection and quantification of tetracycline by use of a whole-cell biosensor in the rat intestine
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Antimicrob Agents Chemother 48:1112-7. 2004
  4. pmc Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella
    Lars Behrendt
    Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark
    ISME J 6:1222-37. 2012
  5. doi request reprint Construction and use of flow cytometry optimized plasmid-sensor strains
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 532:257-68. 2009
  6. doi request reprint Persistence mechanisms of conjugative plasmids
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 532:73-102. 2009
  7. doi request reprint Type 3 fimbriae, encoded by the conjugative plasmid pOLA52, enhance biofilm formation and transfer frequencies in Enterobacteriaceae strains
    Mette Burmølle
    Department of Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Microbiology 154:187-95. 2008
  8. doi request reprint Investigating the diversity of pseudomonas spp. in soil using culture dependent and independent techniques
    Lili Li
    Section for Microbiology, Department of Biology, University of Copenhagen, Denmark
    Curr Microbiol 67:423-30. 2013
  9. pmc Effect of tetracycline on transfer and establishment of the tetracycline-inducible conjugative transposon Tn916 in the guts of gnotobiotic rats
    Martin Iain Bahl
    Department of General Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Appl Environ Microbiol 70:758-64. 2004
  10. ncbi request reprint Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    FEMS Microbiol Lett 253:201-5. 2005

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Studying plasmid horizontal transfer in situ: a critical review
    Søren J Sørensen
    Department of Microbiology, Institute of Biology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Nat Rev Microbiol 3:700-10. 2005
    ..Single-cell detection of donor, recipient and transconjugant bacteria in various natural environments, combined with individual-based mathematical models, has provided a new platform for HGT studies...
  2. ncbi request reprint Making bio-sense of toxicity: new developments in whole-cell biosensors
    Søren J Sørensen
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Curr Opin Biotechnol 17:11-6. 2006
    ....
  3. pmc In vivo detection and quantification of tetracycline by use of a whole-cell biosensor in the rat intestine
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Antimicrob Agents Chemother 48:1112-7. 2004
    ..4% of the intake concentration. This is a significant finding which will help to clarify antimicrobial therapy in the intestinal environment...
  4. pmc Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella
    Lars Behrendt
    Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark
    ISME J 6:1222-37. 2012
    ..In particular, we show that microenvironments and microbial diversity can vary significantly over scales of a few millimeters in such habitats; which is information easily lost by bulk sampling...
  5. doi request reprint Construction and use of flow cytometry optimized plasmid-sensor strains
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 532:257-68. 2009
    ....
  6. doi request reprint Persistence mechanisms of conjugative plasmids
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 532:73-102. 2009
    ..Finally, various molecular adaptations of plasmids to better match the genetic background of their bacterial host cell will be described...
  7. doi request reprint Type 3 fimbriae, encoded by the conjugative plasmid pOLA52, enhance biofilm formation and transfer frequencies in Enterobacteriaceae strains
    Mette Burmølle
    Department of Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Microbiology 154:187-95. 2008
    ..pOLA52 is believed to represent the first example of a conjugative plasmid encoding type 3 fimbriae, resulting in enhanced conjugation frequencies and biofilm formation of the plasmid-harbouring strain...
  8. doi request reprint Investigating the diversity of pseudomonas spp. in soil using culture dependent and independent techniques
    Lili Li
    Section for Microbiology, Department of Biology, University of Copenhagen, Denmark
    Curr Microbiol 67:423-30. 2013
    ..This indicates that NAA 1:100 medium is highly selective for Pseudomonas species, and reveals the ability of NAA 1:100 medium to culture mostly the dominant Pseudomonas species in soil...
  9. pmc Effect of tetracycline on transfer and establishment of the tetracycline-inducible conjugative transposon Tn916 in the guts of gnotobiotic rats
    Martin Iain Bahl
    Department of General Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Appl Environ Microbiol 70:758-64. 2004
    ..This suggests the existence of tetracycline-depleted microhabitats in the intestinal environment...
  10. ncbi request reprint Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    FEMS Microbiol Lett 253:201-5. 2005
    ..The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ngml(-1) to 16 microgml(-1), which represents a significant improvement of the original version...
  11. ncbi request reprint Acclimation of subsurface microbial communities to mercury
    Julia R de Lipthay
    Department of Biology, University of Copenhagen, Copenhagen, Denmark
    FEMS Microbiol Ecol 65:145-55. 2008
    ..The data in this study suggest a role for IncP-1 plasmids in the acclimation to mercury of surface as well as subsurface soil microbial communities...
  12. ncbi request reprint The prevalence of the OqxAB multidrug efflux pump amongst olaquindox-resistant Escherichia coli in pigs
    Lars Hestbjerg Hansen
    Department of Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Microb Drug Resist 11:378-82. 2005
    ..Furthermore, horizontal transfer of olaquindox resistance from three olaquindox-resistant isolates was achieved using an olaquindox-sensitive E. coli as recipient...
  13. ncbi request reprint A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments
    Anders Norman
    Department of Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Mutat Res 603:164-72. 2006
    ..5 ng/g of soil...
  14. pmc Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium Acaryochloris marina
    Lars Behrendt
    Department of Biology, University of Copenhagen, Helsingør, Denmark
    Appl Environ Microbiol 78:3896-904. 2012
    ..A. marina is well adapted to a biofilm growth mode under both visible and NIR irradiance and under O(2) conditions ranging from anoxia to hyperoxia, explaining its presence in natural niches with similar environmental conditions...
  15. ncbi request reprint All IncP-1 plasmid subgroups, including the novel epsilon subgroup, are prevalent in the influent of a Danish wastewater treatment plant
    Martin Iain Bahl
    Department of Biology, University of Copenhagen, Denmark
    Plasmid 62:134-9. 2009
    ..Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes...
  16. doi request reprint The ability of soil bacteria to receive the conjugative IncP1 plasmid, pKJK10, is different in a mixed community compared to single strains
    Claudia I de la Cruz-Perera
    Department of Biology, University of Copenhagen, Copenhagen, Denmark
    FEMS Microbiol Lett 338:95-100. 2013
    ..Our results indicate that the presence of a bacterial community impacts the plasmid permissiveness by affecting the ability of strains to receive the conjugative plasmid...
  17. pmc Sequencing of IncX-plasmids suggests ubiquity of mobile forms of a biofilm-promoting gene cassette recruited from Klebsiella pneumoniae
    Mette Burmølle
    Department of Biology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 7:e41259. 2012
    ....
  18. pmc Conjugative plasmid conferring resistance to olaquindox
    Anders Hay Sørensen
    Department of General Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Antimicrob Agents Chemother 47:798-9. 2003
    ..It also confers resistance to ampicillin and chloramphenicol and has a high frequency of transfer between strains of E. coli. Plasmid-borne olaquindox resistance has not been demonstrated before...
  19. ncbi request reprint Impact of conjugal transfer on the stability of IncP-1 plasmid pKJK5 in bacterial populations
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Sølvgade, Copenhagen K, Denmark
    FEMS Microbiol Lett 266:250-6. 2007
    ..Furthermore, conjugation-proficient plasmids were able to spread infectiously in the bacterial mats initiated with various ratios of plasmid-harboring cells, resulting in a nearly exclusively plasmid-harboring population...
  20. pmc Conjugative transfer facilitates stable maintenance of IncP-1 plasmid pKJK5 in Escherichia coli cells colonizing the gastrointestinal tract of the germfree rat
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, DK 1307 Copenhagen K, Denmark
    Appl Environ Microbiol 73:341-3. 2007
    ..Results show that the plasmid's ability to conjugate counteracts plasmid loss and is thus an important mechanism for the stable maintenance of IncP-1 plasmids within the gastrointestinal environment...
  21. pmc Enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms
    Mette Burmølle
    Department of Microbiology, University of Copenhagen, Copenhagen, Denmark
    Appl Environ Microbiol 72:3916-23. 2006
    ..In summary, our data strongly indicate that synergistic effects promote biofilm biomass and resistance of the biofilm to antimicrobial agents and bacterial invasion in multispecies biofilms...
  22. doi request reprint Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07
    Nadja Larsen
    Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    FEMS Microbiol Ecol 75:482-96. 2011
    ..The main conclusion of the study is that administration of L. acidophilus NCFM and B. lactis Bi-07 does not affect the composition and diversity of the main bacterial populations in feces...
  23. doi request reprint Sequence and comparative analysis of Leuconostoc dairy bacteriophages
    Witold Kot
    Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK 1958 Frederiksberg, Denmark
    Int J Food Microbiol 176:29-37. 2014
    ..No lysogeny modules were detected. To our knowledge this report provides the first comparative genomic work done on Leuconostoc dairy phages...
  24. ncbi request reprint Simultaneous pyrosequencing of the 16S rRNA, IncP-1 trfA, and merA genes
    Peter N Holmsgaard
    Section for Microbiology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark Section for Microbiology, Universitetsparken 15, Building 1, DK 2100 Copenhagen OE, Denmark
    J Microbiol Methods 95:280-4. 2013
    ....
  25. pmc Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere
    Sanin Musovic
    Dept of Microbiology, University of Copenhagen, Institute of Biology, Sølvgade 83H, 1307K Copenhagen K, Denmark
    Appl Environ Microbiol 72:6687-92. 2006
    ..03 x 10(-2) +/- 3.84 x 10(-2). This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ...
  26. pmc Selection for Cu-tolerant bacterial communities with altered composition, but unaltered richness, via long-term Cu exposure
    Jeanette Berg
    Department of Agriculture and Ecology, University of Copenhagen, Frederiksberg, Denmark
    Appl Environ Microbiol 78:7438-46. 2012
    ..Our report highlights several bacterial taxa responding to Cu and thereby provides new guidelines for future studies aiming to explore the bacterial domain for members of metal-responding taxa...
  27. doi request reprint The fate of indigenous microbiota, starter cultures, Escherichia coli, Listeria innocua and Staphylococcus aureus in Danish raw milk and cheeses determined by pyrosequencing and quantitative real time (qRT)-PCR
    Wafa Masoud
    Department of Food Science, Food Microbiology, University of Copenhagen, Rolighedsvej 30, DK 1958 Frederiksberg C, Denmark
    Int J Food Microbiol 153:192-202. 2012
    ..The use of 16S rRNA gene pyrosequencing and qRT-PCR allows a deeper understanding of the behavior of indigenous microbiota, starter cultures and pathogenic bacteria in raw milk and cheeses...
  28. pmc Conjugative plasmids: vessels of the communal gene pool
    Anders Norman
    Department of Biology, Section for Evolution and Microbiology, University of Copenhagen, Copenhagen K, Denmark
    Philos Trans R Soc Lond B Biol Sci 364:2275-89. 2009
    ..This communal pool provides the prokaryotes with an important mechanistic framework for obtaining adaptability and functional diversity that alleviates the need for large genomes of specialized 'private genes'...
  29. pmc Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters
    Anders Norman
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Appl Environ Microbiol 71:2338-46. 2005
    ....
  30. ncbi request reprint High-Throughput Screening of Multispecies Biofilm Formation and Quantitative PCR-Based Assessment of Individual Species Proportions, Useful for Exploring Interspecific Bacterial Interactions
    Dawei Ren
    Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
    Microb Ecol 68:146-54. 2014
    ....
  31. doi request reprint Design of growth-dependent biosensors based on destabilized GFP for the detection of physiological behavior of Escherichia coli in heterogeneous bioreactors
    Shanshan Han
    Dept of Biology, Section of Microbiology, University of Copenhagen, Universitetsparken 15, DK, 2100, Denmark
    Biotechnol Prog 29:553-63. 2013
    ..This investigation is useful for a better understanding of the fast dynamic phenomena occurring in heterogeneous large-scale bioreactors...
  32. pmc Plasmid-encoded multidrug efflux pump conferring resistance to olaquindox in Escherichia coli
    Lars Hestbjerg Hansen
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Antimicrob Agents Chemother 48:3332-7. 2004
    ..coli strain N43. In addition, we show that the efflux system is dependent on the host TolC outer membrane protein when expressed in E. coli...
  33. ncbi request reprint Establishment and early succession of a multispecies biofilm composed of soil bacteria
    Mette Burmølle
    Department of Microbiology, University of Copenhagen, Copenhagen K, Denmark
    Microb Ecol 54:352-62. 2007
    ..Thus, the biofilm flow model proved useful for investigations of how intrinsic phenotypic traits of individual species affect the succession in an early soil biofilm consortium...
  34. pmc Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults
    Nadja Larsen
    Department of Food Science, University of Copenhagen, Frederiksberg, Denmark
    PLoS ONE 5:e9085. 2010
    ..The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control...
  35. ncbi request reprint The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established alpha, beta and delta sub-groups
    Martin Iain Bahl
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Plasmid 58:31-43. 2007
    ....
  36. pmc Design and synthesis of a quintessential self-transmissible IncX1 plasmid, pX1.0
    Lars H Hansen
    Department of Biology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 6:e19912. 2011
    ..Our results demonstrate the immediate applicability of recent advances made within the field of synthetic biology for designing and constructing DNA systems, previously existing only in silica...
  37. pmc Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes
    Tim Holm Jakobsen
    Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 8:e68484. 2013
    ..Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of A. xylosoxidans and thereby improves our understanding and ability to treat this emerging pathogen. ..
  38. pmc Rapid TaqMan-based quantification of chlorophyll d-containing cyanobacteria in the genus Acaryochloris
    Lars Behrendt
    Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark
    Appl Environ Microbiol 80:3244-9. 2014
    ..The new TaqMan assay allows quick and easy screening of environmental samples for the presence of Acaryochloris species and is an important tool to further resolve the global distribution and significance of this unique oxyphototroph...
  39. pmc Gut microbiota composition is correlated to grid floor induced stress and behavior in the BALB/c mouse
    Katja Maria Bangsgaard Bendtsen
    Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 7:e46231. 2012
    ..It is concluded that grid floor housing changes the GM composition, which seems to influence certain anxiety-related parameters...
  40. pmc Bioinformatic approaches reveal metagenomic characterization of soil microbial community
    Zhuofei Xu
    Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 9:e93445. 2014
    ..Our results together with the established bioinformatic pipelines should provide a foundation for future research into the relation between soil biodiversity and ecosystem function. ..
  41. ncbi request reprint Direct detection and quantification of horizontal gene transfer by using flow cytometry and gfp as a reporter gene
    Søren J Sørensen
    Department of General Microbiology, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83 H, DK 1307, Copenhagen, Denmark
    Curr Microbiol 47:129-33. 2003
    ..The method presented here provided simple and precise quantification of horizontal gene transfer between both Escherichia coli and Pseudomonas putida strains...
  42. pmc Endolithic chlorophyll d-containing phototrophs
    Lars Behrendt
    Marine Biological Laboratory, Department of Biology, University of Copenhagen, Helsingør, Denmark
    ISME J 5:1072-6. 2011
    ..This finding suggests an important role of Chl d-containing cyanobacteria in a range of hitherto unexplored endolithic habitats, where NIR light-driven oxygenic photosynthesis may be significant...
  43. ncbi request reprint Biofilms in chronic infections - a matter of opportunity - monospecies biofilms in multispecies infections
    Mette Burmølle
    Department of Biology, University of Copenhagen, Denmark
    FEMS Immunol Med Microbiol 59:324-36. 2010
    ..We discuss these differences from both the diagnostic and the scientific point of view...
  44. ncbi request reprint The cda GenoTox assay: a new and sensitive method for detection of environmental genotoxins, including nitroarenes and aromatic amines
    Trine G Østergaard
    Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark
    Mutat Res 631:77-84. 2007
    ..This resulted in an assay that was able to detect the nitroarene 1-nitropyrene and the aromatic amine 2-aminoanthracene with high sensitivity...