M Ibba

Summary

Affiliation: University of Copenhagen
Country: Denmark

Publications

  1. ncbi Aminoacyl-tRNA synthesis
    M Ibba
    Center for Biomolecular Recognition, IMBG Laboratory B, The Panum Institute, DK 2200, Copenhagen N, Denmark
    Annu Rev Biochem 69:617-50. 2000
  2. pmc Transfer RNA identity contributes to transition state stabilization during aminoacyl-tRNA synthesis
    M Ibba
    Department of Molecular Biophysics and Biochemistry Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 27:3631-7. 1999
  3. pmc A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity
    A J Lloyd
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 23:2886-92. 1995
  4. pmc Interactions between tRNA identity nucleotides and their recognition sites in glutaminyl-tRNA synthetase determine the cognate amino acid affinity of the enzyme
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 93:6953-8. 1996
  5. ncbi One polypeptide with two aminoacyl-tRNA synthetase activities
    C Stathopoulos
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Science 287:479-82. 2000
  6. pmc Archaeal aminoacyl-tRNA synthesis: diversity replaces dogma
    D Tumbula
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8114, USA
    Genetics 152:1269-76. 1999
  7. pmc Transfer RNA-dependent cognate amino acid recognition by an aminoacyl-tRNA synthetase
    K W Hong
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    EMBO J 15:1983-91. 1996
  8. pmc Substrate recognition by class I lysyl-tRNA synthetases: a molecular basis for gene displacement
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 96:418-23. 1999
  9. pmc The renaissance of aminoacyl-tRNA synthesis
    M Ibba
    Center for Biomolecular Recognition, Department of Medical Biochemistry and Genetics, Laboratory B, The Panum Institute, Blegdamsvej 3C, DK 2200, Copenhagen N
    EMBO Rep 2:382-7. 2001
  10. pmc Context-dependent anticodon recognition by class I lysyl-tRNA synthetases
    D Soll
    Center for Biomolecular Recognition, IMBG Laboratory B, The Panum Institute, Blegdamsvej 3C, DK 2200N, Copenhagen, Denmark
    Proc Natl Acad Sci U S A 97:14224-8. 2000

Collaborators

Detail Information

Publications19

  1. ncbi Aminoacyl-tRNA synthesis
    M Ibba
    Center for Biomolecular Recognition, IMBG Laboratory B, The Panum Institute, DK 2200, Copenhagen N, Denmark
    Annu Rev Biochem 69:617-50. 2000
    ..This article reviews current knowledge of the biochemical, structural, and evolutionary facets of aminoacyl-tRNA synthesis...
  2. pmc Transfer RNA identity contributes to transition state stabilization during aminoacyl-tRNA synthesis
    M Ibba
    Department of Molecular Biophysics and Biochemistry Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 27:3631-7. 1999
    ..These results directly show that tRNA identity contributes to the degree of complementarity to the transition state for tRNA charging in the active site of an aminoacyl-tRNA synthetase:aminoacyl-adenylate:tRNA complex...
  3. pmc A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity
    A J Lloyd
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 23:2886-92. 1995
    ..Finally, this novel method was used to provide direct evidence for the cooperativity of tRNA and ATP binding to GlnRS...
  4. pmc Interactions between tRNA identity nucleotides and their recognition sites in glutaminyl-tRNA synthetase determine the cognate amino acid affinity of the enzyme
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 93:6953-8. 1996
    ..The ability of tRNA to optimize amino acid recognition reveals a novel mechanism for maintaining translational fidelity and also provides a strong basis for the coevolution of tRNAs and their cognate synthetases...
  5. ncbi One polypeptide with two aminoacyl-tRNA synthetase activities
    C Stathopoulos
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Science 287:479-82. 2000
    ..The ability of one enzyme to provide two aminoacyl-tRNAs for protein synthesis raises questions about concepts of substrate specificity in protein synthesis and may provide insights into the evolutionary origins of this process...
  6. pmc Archaeal aminoacyl-tRNA synthesis: diversity replaces dogma
    D Tumbula
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8114, USA
    Genetics 152:1269-76. 1999
    ....
  7. pmc Transfer RNA-dependent cognate amino acid recognition by an aminoacyl-tRNA synthetase
    K W Hong
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    EMBO J 15:1983-91. 1996
    ..The observed role of RNA as a cofactor in optimizing amino acid activation suggests that the tRNAGln-GlnRS complex may be partly analogous to ribonucleoprotein enzymes where protein-RNA interactions facilitate catalysis...
  8. pmc Substrate recognition by class I lysyl-tRNA synthetases: a molecular basis for gene displacement
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 96:418-23. 1999
    ....
  9. pmc The renaissance of aminoacyl-tRNA synthesis
    M Ibba
    Center for Biomolecular Recognition, Department of Medical Biochemistry and Genetics, Laboratory B, The Panum Institute, Blegdamsvej 3C, DK 2200, Copenhagen N
    EMBO Rep 2:382-7. 2001
    ..As a more complete picture of aminoacyl-tRNA synthesis now begins to emerge, the high degree of evolutionary diversity in this universal and essential process is becoming clearer...
  10. pmc Context-dependent anticodon recognition by class I lysyl-tRNA synthetases
    D Soll
    Center for Biomolecular Recognition, IMBG Laboratory B, The Panum Institute, Blegdamsvej 3C, DK 2200N, Copenhagen, Denmark
    Proc Natl Acad Sci U S A 97:14224-8. 2000
    ..Such patterns of competition also provide a basis for the two separate horizontal gene transfer events hypothesized in the evolution of the class I lysyl-tRNA synthetases...
  11. ncbi Glutaminyl-tRNA synthetase: from genetics to molecular recognition
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Genes Cells 1:421-7. 1996
    ..This mechanism now provides a ready explanation as to why the majority of tRNA mischarging events, including those originally described over 25 years ago for GlnRS, impair cellular viability only to a limited degree...
  12. ncbi The terminal adenosine of tRNA(Gln) mediates tRNA-dependent amino acid recognition by glutaminyl-tRNA synthetase
    J Liu
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Biochemistry 37:9836-42. 1998
    ..These data now show that Asp66, Tyr211 and Phe233 mediate tRNA-dependent cognate amino acid recognition via the invariant 3'-terminal adenosine of tRNA(Gln)...
  13. ncbi Homologous expression and purification of mutants of an essential protein by reverse epitope-tagging
    H U Thomann
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA
    Biotechnology (N Y) 14:50-5. 1996
    ....
  14. ncbi Genomics-based identification of targets in pathogenic bacteria for potential therapeutic and diagnostic use
    G Raczniak
    Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 208114, 266 Whitney Avenue, New Haven, CT 06520-8114, USA
    Toxicology 160:181-9. 2001
    ..In particular, the integration of genomic and biochemical approaches to identify novel targets for the treatment of Chlamydial infections and the diagnosis and treatment of Lyme disease will be presented...
  15. ncbi Genomics and the evolution of aminoacyl-tRNA synthesis
    B Ruan
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA
    Acta Biochim Pol 48:313-21. 2001
    ..Characterization of non-canonical aminoacyl-tRNA synthesis has revealed an unexpected level of evolutionary divergence and has also provided new insights into the possible precursors of contemporary aminoacyl-tRNA synthetases...
  16. pmc Archaeal-type lysyl-tRNA synthetase in the Lyme disease spirochete Borrelia burgdorferi
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 94:14383-8. 1997
    ..This difference between the lysyl-tRNA synthetases of spirochetes and their hosts may be readily exploitable for the development of anti-spirochete therapeutics...
  17. ncbi Protein synthesis: twenty three amino acids and counting
    M Ibba
    Center for Biomolecular Recognition, Department of Medical Biochemistry and Genetics, Laboratory B, The Panum Institute, Blegdamsvej 3C, DK-2200, Copenhagen N, Denmark
    Curr Biol 11:R563-5. 2001
    ..Recent advances at the interface of chemistry and molecular biology are extending the genetic code to allow assignment of new amino acids to existing codons, providing new functional groups for protein synthesis...
  18. ncbi A euryarchaeal lysyl-tRNA synthetase: resemblance to class I synthetases
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Yale University, Post Office Box 208114, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    Science 278:1119-22. 1997
    ..These data question the concept that the classification of aminoacyl-tRNA synthetases does not vary throughout living systems...
  19. ncbi Substrate specificity is determined by amino acid binding pocket size in Escherichia coli phenylalanyl-tRNA synthetase
    M Ibba
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    Biochemistry 33:7107-12. 1994
    ..Tyrosine activation was also improved with this mutant, but the resulting enzyme-Tyr-adenylate complex was rapidly hydrolyzed, indicating the presence of a proofreading mechanism in E. coli phenylalanyl-tRNA synthetase...