Lise Lotte Hansen

Summary

Affiliation: University of Aarhus
Country: Denmark

Publications

  1. ncbi request reprint Assignment of the human peptide chain release factor 3 (GSPT2) to Xp11.23-->p11.21 and of the distal marker DXS1039 by radiation hybrid mapping
    L L Hansen
    Institute of Human Genetics, Aarhus University, Aarhus, Denmark
    Cytogenet Cell Genet 86:250-1. 1999
  2. ncbi request reprint Assignment of the human translation termination factor 1 (ETF1) to 5q31.1 and of the proximal marker D5S1995 by radiation hybrid mapping
    L L Hansen
    Institute of Human Genetics, Aarhus University, Aarhus, Denmark
    Cytogenet Cell Genet 87:256-7. 1999
  3. ncbi request reprint Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping
    L L Hansen
    Institute of Human Genetics, Aarhus University, Denmark
    Cytogenet Cell Genet 88:91-2. 2000
  4. ncbi request reprint Assignment of human NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (NDUFB3) and of its four pseudogenes to human chromosomes 2q31.3, 1p13.3-->p13.1, 9q32-->q34.1, 14q22.3-->q23.1 and 14q32.2 by radiation hybrid mapping
    L L Jensen
    Institute of Human Genetics, Bartholin Building, Aarhus University, DK 8000 Aarhus C, Denmark
    Cytogenet Cell Genet 93:147-50. 2001
  5. ncbi request reprint Allelic imbalance in selected chromosomal regions in ovarian cancer
    Lise Lotte Hansen
    Department of Human Genetics, The Bartholin Building, University of Aarhus, DK 8000 C, Aarhus, Denmark
    Cancer Genet Cytogenet 139:1-8. 2002
  6. ncbi request reprint Conflicting results in SNP genotype assessment
    Lise Lotte Hansen
    Danish Centre for Molecular Gerontology, Institute of Human Genetics, University of Aarhus, Denmark
    Biotechniques 43:756, 758, 760 passim. 2007
  7. pmc A new approach to primer design for the control of PCR bias in methylation studies
    Tomasz K Wojdacz
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, St, Andrews Place, East Melbourne, VIC 3002, Australia
    BMC Res Notes 1:54. 2008
  8. pmc A simple way to evaluate self-designed probes for tumor specific Multiplex Ligation-dependent Probe Amplification (MLPA)
    Kristina Pedersen
    Institute of Human Genetics, The Bartholin Building, Wilhelm Meyers Allé 4, University of Aarhus, DK 8000 Aarhus C, Denmark
    BMC Res Notes 3:179. 2010
  9. doi request reprint High-resolution melting analysis for mutation screening of RGSL1, RGS16, and RGS8 in breast cancer
    Emilia Wiechec
    Institute of Human Genetics, University of Aarhus, Aarhus C, Denmark
    Cancer Epidemiol Biomarkers Prev 20:397-407. 2011
  10. doi request reprint A fragile site within the HPC1 region at 1q25.3 affecting RGS16, RGSL1, and RGSL2 in human breast carcinomas
    Emilia Wiechec
    Danish Centre for Molecular Gerontology, Institute of Human Genetics, University of Aarhus, DK 8000 Aarhus C, Denmark
    Genes Chromosomes Cancer 47:766-80. 2008

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Assignment of the human peptide chain release factor 3 (GSPT2) to Xp11.23-->p11.21 and of the distal marker DXS1039 by radiation hybrid mapping
    L L Hansen
    Institute of Human Genetics, Aarhus University, Aarhus, Denmark
    Cytogenet Cell Genet 86:250-1. 1999
  2. ncbi request reprint Assignment of the human translation termination factor 1 (ETF1) to 5q31.1 and of the proximal marker D5S1995 by radiation hybrid mapping
    L L Hansen
    Institute of Human Genetics, Aarhus University, Aarhus, Denmark
    Cytogenet Cell Genet 87:256-7. 1999
  3. ncbi request reprint Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping
    L L Hansen
    Institute of Human Genetics, Aarhus University, Denmark
    Cytogenet Cell Genet 88:91-2. 2000
  4. ncbi request reprint Assignment of human NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (NDUFB3) and of its four pseudogenes to human chromosomes 2q31.3, 1p13.3-->p13.1, 9q32-->q34.1, 14q22.3-->q23.1 and 14q32.2 by radiation hybrid mapping
    L L Jensen
    Institute of Human Genetics, Bartholin Building, Aarhus University, DK 8000 Aarhus C, Denmark
    Cytogenet Cell Genet 93:147-50. 2001
  5. ncbi request reprint Allelic imbalance in selected chromosomal regions in ovarian cancer
    Lise Lotte Hansen
    Department of Human Genetics, The Bartholin Building, University of Aarhus, DK 8000 C, Aarhus, Denmark
    Cancer Genet Cytogenet 139:1-8. 2002
    ..2 approximately q24.3. In this article, we report three new polymorphic microsatellite markers and strong evidence of AL of narrow well-defined regions in hot spots on 6q, 13q, and 16q in ovarian tumors...
  6. ncbi request reprint Conflicting results in SNP genotype assessment
    Lise Lotte Hansen
    Danish Centre for Molecular Gerontology, Institute of Human Genetics, University of Aarhus, Denmark
    Biotechniques 43:756, 758, 760 passim. 2007
    ..The problem we have encountered may originate from specific structures in the genomic DNA sequence, rather than being a methodological problem...
  7. pmc A new approach to primer design for the control of PCR bias in methylation studies
    Tomasz K Wojdacz
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, St, Andrews Place, East Melbourne, VIC 3002, Australia
    BMC Res Notes 1:54. 2008
    ..The focus of this brief communication is to point out the important criteria needed for the successful choice of primers that will enable the control of PCR bias in bisulfite based methylation-screening protocols...
  8. pmc A simple way to evaluate self-designed probes for tumor specific Multiplex Ligation-dependent Probe Amplification (MLPA)
    Kristina Pedersen
    Institute of Human Genetics, The Bartholin Building, Wilhelm Meyers Allé 4, University of Aarhus, DK 8000 Aarhus C, Denmark
    BMC Res Notes 3:179. 2010
    ..During evaluation of new self-designed reference probes, we encountered a number of problems, especially when applying the MLPA methodology to tumor samples...
  9. doi request reprint High-resolution melting analysis for mutation screening of RGSL1, RGS16, and RGS8 in breast cancer
    Emilia Wiechec
    Institute of Human Genetics, University of Aarhus, Aarhus C, Denmark
    Cancer Epidemiol Biomarkers Prev 20:397-407. 2011
    ..We evaluated the use of high-resolution melting (HRM) to screen for mutations in the genes of interest and assess their clinical significance...
  10. doi request reprint A fragile site within the HPC1 region at 1q25.3 affecting RGS16, RGSL1, and RGSL2 in human breast carcinomas
    Emilia Wiechec
    Danish Centre for Molecular Gerontology, Institute of Human Genetics, University of Aarhus, DK 8000 Aarhus C, Denmark
    Genes Chromosomes Cancer 47:766-80. 2008
    ....
  11. doi request reprint Chromosome 1q25.3 copy number alterations in primary breast cancers detected by multiplex ligation-dependent probe amplification and allelic imbalance assays and its comparison with fluorescent in situ hybridization assays
    Emilia Wiechec
    Department of Biomedicine, University of Aarhus, Wilhelm Meyers Allé 4, 8000, Aarhus C, Denmark
    Cell Oncol (Dordr) 36:113-20. 2013
    ..Here, we evaluated the accuracy of 1q25.3 copy number detection in conjunction with allelic imbalance (AI) detection in a series of primary breast tumours...
  12. pmc Methylation of cancer related genes in tumor and peripheral blood DNA from the same breast cancer patient as two independent events
    Tomasz K Wojdacz
    The Lundbeck Foundation Centre for International Research in Radiation Oncology, Nørrebrogade 44, Building NO, 5, Aarhus University Hospital, DK 8000 Aarhus C, Denmark
    Diagn Pathol 6:116. 2011
    ..In this study, we aimed to investigate a relationship between methylation of three breast cancer related genes in PB DNA, and tumor specific (somatic) methylation of these genes in the same individual...
  13. doi request reprint Competitive amplification of differentially melting amplicons (CADMA) enables sensitive and direct detection of all mutation types by high-resolution melting analysis
    Lasse S Kristensen
    Department of Biomedicine, Aarhus University, Denmark
    Hum Mutat 33:264-71. 2012
    ..025% and 0.25%, mutated alleles in a wild-type background. In conclusion, CADMA enables highly sensitive and specific mutation detection by HRM analysis...
  14. doi request reprint Limitations and advantages of MS-HRM and bisulfite sequencing for single locus methylation studies
    Tomasz K Wojdacz
    Institute of Human Genetics, University of Aarhus, Wilhelm Meyers Alle 240, Aarhus C, Denmark
    Expert Rev Mol Diagn 10:575-80. 2010
    ..Furthermore, we provide the insights into interpretation of the results obtained when a combination of the protocols is used for single-locus methylation studies...
  15. doi request reprint Methylation of MGMT in malignant pleural mesothelioma occurs in a subset of patients and is associated with the T allele of the rs16906252 MGMT promoter SNP
    Lasse Sommer Kristensen
    Institute of Human Genetics, Aarhus University, The Bartholin Building, Wilhelm Meyers Allé 4, DK 8000 Aarhus C, Denmark
    Lung Cancer 71:130-6. 2011
    ..In conclusion, methylation of the MGMT promoter occurs in a subset of MPM patients and is associated with the T allele of the MGMT rs16906252 SNP. However, complete silencing of MGMT in MPM is a rare event...
  16. doi request reprint Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products
    Lasse S Kristensen
    Institute of Human Genetics, University of Aarhus, The Bartholin Building, Aarhus C, Denmark
    Hum Mutat 31:1366-73. 2010
    ..In conclusion, COLD-PCR combined with HRM, is a simple way of increasing the sensitivity of KRAS mutation detection without adding to the complexity and cost of the experiments...
  17. pmc Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM
    Lasse S Kristensen
    Institute of Human Genetics, University of Aarhus, The Bartholin Building, Aarhus C, Denmark
    BMC Cancer 9:453. 2009
    ..Sensitive Melting Analysis after Real Time - Methylation Specific PCR (SMART-MSP) and Methylation Sensitive - High Resolution Melting (MS-HRM) are two methods for single locus DNA methylation detection based on HRM...
  18. doi request reprint Evaluation of BRAF mutation testing methodologies in formalin-fixed, paraffin-embedded cutaneous melanomas
    Johanne Lade-Keller
    Institute of Pathology, Aarhus University Hospital, Aarhus, Denmark
    J Mol Diagn 15:70-80. 2013
    ..CADMA and TaqMan may not require macrodissections for a reliable test. Therefore, the use of more sensitive methods may have a future in testing for BRAF mutations in clinical settings...
  19. pmc Competitive amplification of differentially melting amplicons (CADMA) improves KRAS hotspot mutation testing in colorectal cancer
    Lasse Sommer Kristensen
    Department of Biomedicine, University of Aarhus, Bartholin Building, Wilhelm Meyers Allé 4, DK 8000 Aarhus C, Denmark
    BMC Cancer 12:548. 2012
    ..Testing for KRAS mutations is now required prior to anti-EGFR treatment, however, less sensitive methods based on conventional PCR regularly fail to detect KRAS mutations in clinical samples...
  20. doi request reprint Epigenetics and cancer treatment
    Lasse Sommer Kristensen
    Institute of Human Genetics, The Bartholin Building, University of Aarhus, 8000 Aarhus C, Denmark
    Eur J Pharmacol 625:131-42. 2009
    ..Finally, we will discuss the potential of DNA methylation changes as biomarkers to be used in diverse areas of cancer treatment, especially for predicting response to treatment with DNMT and HDAC inhibitors...
  21. ncbi request reprint Primer design versus PCR bias in methylation independent PCR amplifications
    Tomasz K Wojdacz
    Institute of Human Genetics, University of Aarhus, Aarhus C DK 8000, Denmark
    Epigenetics 4:231-4. 2009
    ..In conclusion, the primers with limited number of CpG sites are able to effectively reverse PCR bias and therefore detect methylated templates with significantly higher sensitivity than CpG free primers...
  22. pmc Germline mutation in RNASEL predicts increased risk of head and neck, uterine cervix and breast cancer
    Bo Eskerod Madsen
    Bioinformatics Research Center, University of Aarhus, Aarhus, Denmark
    PLoS ONE 3:e2492. 2008
    ..A genetic predisposition could explain why some viral infections persist and induce cancer, while others disappear spontaneously. This points at RNASEL as a strong susceptibility gene...
  23. doi request reprint Methylation-sensitive high-resolution melting
    Tomasz K Wojdacz
    Institute of Human Genetics, University of Aarhus, The Bartholin Building, Wilhelm Meyers Alle, Bygn 1242, DK 8000 Aarhus C, Denmark
    Nat Protoc 3:1903-8. 2008
    ..Here, we provide a protocol for MS-HRM, which enables highly sensitive, labor- and cost-efficient single-locus methylation studies on the basis of DNA high-resolution melting technology...
  24. ncbi request reprint Reversal of PCR bias for improved sensitivity of the DNA methylation melting curve assay
    Tomasz K Wojdacz
    Institute of Human Genetics, University of Aarhus, Denmark
    Biotechniques 41:274, 276, 278. 2006
  25. doi request reprint The effect of genetic variability on drug response in conventional breast cancer treatment
    Emilia Wiechec
    Institute of Human Genetics, The Bartholin Building, University of Aarhus, DK 8000 Aarhus C, Denmark
    Eur J Pharmacol 625:122-30. 2009
    ..In this review the focus is on clinically relevant SNPs in genes implicated in drug metabolism and disposition as well as their influence on breast cancer therapy toxicity and/or efficacy...
  26. doi request reprint Identification of accurate reference genes for RT-qPCR analysis of formalin-fixed paraffin-embedded tissue from primary non-small cell lung cancers and brain and lymph node metastases
    Signe Søes
    Department of Biomedicine, Aarhus University, The Bartholin Building, Wilhelm Meyers Allé 4, DK 8000 Aarhus C, Denmark
    Lung Cancer 81:180-6. 2013
    ..The presented workflow and the identified reference genes may facilitate more reliable gene expression studies in lung cancer using RNA from FFPE tissues...
  27. ncbi request reprint Deconstructing PTI-1: PTI-1 is a truncated, but not mutated, form of translation elongatin factor 1A1, eEF1A1
    Francisco Mansilla
    Department of Molecular Biology, University of Aarhus, Arhus C, Denmark
    Biochim Biophys Acta 1727:116-24. 2005
    ..However, we were unable to detect the PTI-1 protein in LNCaP cell extracts. The potential roles of the PTI-1 protein in carcinogenesis and the origin of the PTI-1 gene in the human genome are discussed...
  28. doi request reprint PCR-based methods for detecting single-locus DNA methylation biomarkers in cancer diagnostics, prognostics, and response to treatment
    Lasse Sommer Kristensen
    Institute of Human Genetics, University of Aarhus, Aarhus, Denmark
    Clin Chem 55:1471-83. 2009
    ..Some of these genes are considered promising DNA methylation biomarkers for early cancer diagnostics, and some have been shown to be valuable for predicting prognosis or the response to therapy...
  29. ncbi request reprint Techniques used in studies of age-related DNA methylation changes
    Tomasz K Wojdacz
    The Danish Centre for Molecular Gerontology, Institute of Human Genetics, University of Aarhus, Denmark
    Ann N Y Acad Sci 1067:479-87. 2006
    ..The new technique will serve as an efficient tool in understanding the nature of epigenetic changes and their significance to the aging process and cancer development...
  30. ncbi request reprint A nine-nucleotide deletion and splice variation in the coding region of the interferon induced ISG12 gene
    Kamille C J Smidt
    Department of Molecular Biology, University of Aarhus, C F Mollers Allé Building 130, 8000 C, Aarhus, Denmark
    Biochim Biophys Acta 1638:227-34. 2003
    ..In heterozygous cells from cytobrush material with neoplastic lesions, we found a preference for expression of the ISG12(0) allele...